1.
Presse Med
; 26(10): 464-5, 1997 Mar 29.
Artículo
en Francés
| MEDLINE
| ID: mdl-9137369
2.
J Capillary Electrophor
; 2(4): 197-202, 1995.
Artículo
en Inglés
| MEDLINE
| ID: mdl-9384774
RESUMEN
CE has been employed for peptide mapping characterization of the light chain of a human anti-Rhesus (D) (Rh[D]) antibody presently undergoing clinical evaluation. In the presence of an ion-pairing agent used to increase resolution, reproducible maps were obtained within 55 min after injection of 12 fmol of protein. CE has also been employed as a direct and quick screening tool for purity evaluation of the tryptic peptides obtained from reversed-phase high-performance liquid chromatography (RP-HPLC) prior to sequence analysis. Post-translational modifications of the protein were determined by identification of the cysteine residues implicated in disulfide linkages.