On the question of the internal frame.

This paper attempts to expand José Bleger's classic, metapsychological descriptions of the psychoanalytic frame to formulate and emphasize the role of the analyst's internal frame in establishing a psychoanalytic observational perspective in the analytic situation. The rationale for doing so follows from clinical necessity, especially when working with patients and psychic organizations that are 'beyond neurosis' and in non-traditional settings such as distance and telemetric analyses. Clinically speaking, in its most effective state, the analyst's internal frame can inform the possibility of an observational vertex aimed at the intuitive grasp of psychic reality rather than a sense-based, empirical observation of parameters denoted by the elements of a consensually validatable social reality.

To Feel in My Flesh: Receptivity, Resonance, Representation, and The Beta Screen.

When we are confronted with the challenge of trying to fully convey or describe something about human life and emotional experience, we find ourselves up against the very limitations of language. This problem becomes especially relevant as we attempt to expand psychoanalytic theory so as to enable us to "approach a mental life unmapped by the theories elaborated for the understanding of neurosis" (Bion1962, p. 37). This paper seeks to aid in that expansion by revisiting Bion's early writings about the beta screen, extending his conclusions about communication from the psychotic part of the mind to the broad area of the unrepresented (the unstructured unconscious), suggesting that there is often a potentially communicative meaning, a mute plea for intersubjective regulatory assistance (alpha function), embedded in the unconscious evocation of emotions in the object and that this cry for help may be encrypted in even the most seemingly destructive, resistant and oppositional patients.

On looking into The Ego and the Id 100 years after its publication.

Freud's publication of The Ego and the Id sparked a diverging set of psychoanalytic models - ego psychology, structural conflict theory, Kleinianism, object relations theories, Lacanianism, etc. - each of which attempted to deal with the clinical limitations of his first topography in regard to unconscious guilt, negative therapeutic reactions and primitive character organizations. This paper attempts to look back on these developments from the perspective of contemporary, post-Freudian psychoanalytic theories.

A Metapsychology of the Unrepresented.

The terms unrepresented and unrepresented states are increasingly being referred to in psychoanalytic discourse, without our having established a generally agreed upon consensus about their definition, use or meaning. While these particular designations were never used by Freud, a careful reading of his work reveals them to be qualities that characterize the initial state of both the drive and perception. This paper attempts to place these terms in a clinically useful, metapsychological perspective by reviewing their conceptual origin in Freud and examining their elaboration and clinical relevance in the work of Bion, Winnicott, and Green. These concepts should prove especially useful for understanding and addressing problems presented by non-neurotic patients and psychic organizations and will help expand the reach and efficacy of psychoanalytic understanding and technique to increasing numbers of contemporary patients.

Brachypodium distachyon Seedlings Display Accession-Specific Morphological and Transcriptomic Responses to the Microgravity Environment of the International Space Station.

Plants have been recognized as key components of bioregenerative life support systems for space exploration, and many experiments have been carried out to evaluate their adaptability to spaceflight. Unfortunately, few of these experiments have involved monocot plants, which constitute most of the crops used on Earth as sources of food, feed, and fiber. To better understand the ability of monocot plants to adapt to spaceflight, we germinated and grew Brachypodium distachyon seedlings of the Bd21, Bd21-3, and Gaz8 accessions in a customized growth unit on the International Space Station, along with 1-g ground controls. At the end of a 4-day growth period, seedling organ's growth and morphologies were quantified, and root and shoot transcriptomic profiles were investigated using RNA-seq. The roots of all three accessions grew more slowly and displayed longer root hairs under microgravity conditions relative to ground control. On the other hand, the shoots of Bd21-3 and Gaz-8 grew at similar rates between conditions, whereas those of Bd21 grew more slowly under microgravity. The three Brachypodium accessions displayed dramatically different transcriptomic responses to microgravity relative to ground controls, with the largest numbers of differentially expressed genes (DEGs) found in Gaz8 (4527), followed by Bd21 (1353) and Bd21-3 (570). Only 47 and six DEGs were shared between accessions for shoots and roots, respectively, including DEGs encoding wall-associated proteins and photosynthesis-related DEGs. Furthermore, DEGs associated with the "Oxidative Stress Response" GO group were up-regulated in the shoots and down-regulated in the roots of Bd21 and Gaz8, indicating that Brachypodium roots and shoots deploy distinct biological strategies to adapt to the microgravity environment. A comparative analysis of the Brachypodium oxidative-stress response DEGs with the Arabidopsis ROS wheel suggests a connection between retrograde signaling, light response, and decreased expression of photosynthesis-related genes in microgravity-exposed shoots. In Gaz8, DEGs were also found to preferentially associate with the "Plant Hormonal Signaling" and "MAP Kinase Signaling" KEGG pathways. Overall, these data indicate that Brachypodium distachyon seedlings exposed to the microgravity environment of ISS display accession- and organ-specific responses that involve oxidative stress response, wall remodeling, photosynthesis inhibition, expression regulation, ribosome biogenesis, and post-translational modifications. The general characteristics of these responses are similar to those displayed by microgravity-exposed Arabidopsis thaliana seedlings. However, organ- and accession-specific components of the response dramatically differ both within and between species. These results suggest a need to directly evaluate candidate-crop responses to microgravity to better understand their specific adaptability to this novel environment and develop cultivation strategies allowing them to strive during spaceflight.

Safety of a New Sinus Irrigation Device in Rhinosinusitis: A Pilot Study.

BACKGROUND: Puncture and lavage of the paranasal sinuses, previously the primary treatment for unresponsive acute bacterial rhinosinusitis before surgery, has been abandoned due to procedural discomfort and advancements in antibiotic efficacy and endoscopic surgery. The rise in antibiotic-resistant bacteria has renewed the interest in minimally invasive sinus lavage to both avoid aggressive surgical interventions and identify appropriate antibiotic therapy. In this article, we describe the safety and feasibility of a new device in human patients and evaluate its efficacy as a treatment before the traditional sinus surgery in acute rhinosinusitis. METHODS: The device with its seeker-shaped guiding tube and rotating wire can enter the sinus cavity through the natural ostium, pulverize the inspissated mucus, and enable lavage and culture sampling without the need for sinus puncturing. It was tested in 6 patients with chronic sinusitis under general anesthesia during endoscopic sinus surgery and in additional 10 patients with maxillary acute bacterial rhinosinusitis in outpatient settings under local anesthesia. RESULTS: The device enabled rapid, efficient, and atraumatic insertion of the wire into the occluded sinuses. The rotating wire permitted pulverization of the thick mucus, which enabled irrigation without mucosal damage or adverse events. Overall, 9 of 10 patients with acute bacterial rhinosinusitis demonstrated remarkable improvements and were discharged the following day with no acute symptoms. The visual analog scale score for pain dropped from 8.9 to 0.4. The remaining one patient underwent endoscopic sinus surgery subsequently. None of the patients treated during endoscopic sinus surgery developed any adverse events.

Simulated galactic cosmic ray exposure activates dose-dependent DNA repair response and down regulates glucosinolate pathways in arabidopsis seedlings.

Outside the protection of Earth's magnetic field, organisms are constantly exposed to space radiation consisting of energetic protons and other heavier charged particles. With the goal of crewed Mars exploration, the production of fresh food during long duration space missions is critical for meeting astronauts' nutritional and psychological needs. However, the biological effects of space radiation on plants have not been sufficiently investigated and characterized. To that end, 10-day-old Arabidopsis seedlings were exposed to simulated Galactic Cosmic Rays (GCR) and assessed for transcriptomic changes. The simulated GCR irradiation was carried out in the NASA Space Radiation Laboratory (NSRL) at Brookhaven National Lab (BNL). The exposures were conducted acutely for two dose points at 40 cGy or 80 cGy, with sequential delivery of proton, helium, oxygen, silicon, and iron ions. Control and irradiated seedlings were then harvested and preserved in RNAlater at 3 hrs post irradiation. Total RNA was isolated for transcriptomic analyses using RNAseq. The data revealed that the transcriptomic responses were dose-dependent, with significant upregulation of DNA repair pathways and downregulation of glucosinolate biosynthetic pathways. Glucosinolates are important for plant pathogen defense and for the taste of a plant, which are both relevant to growing plants for spaceflight. These findings fill in knowledge gaps of how plants respond to radiation in beyond-Earth environments.

Response of Arabidopsis thaliana and Mizuna Mustard Seeds to Simulated Space Radiation Exposures.

One of the major concerns for long-term exploration missions beyond the Earth's magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the production of fresh food in space will provide both nutritional supplements and psychological benefits to the astronauts. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of two different compositions of charged particles-simulated GCR, and simulated SPE protons on dry and hydrated seeds of the model plant Arabidopsis thaliana and the crop plant Mizuna mustard [Brassica rapa var. japonica]. Exposures to charged particles, simulated GCRs (up to 80 cGy) or SPEs (up to 200 cGy), were performed either acutely or at a low dose rate using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Control and irradiated seeds were planted in a solid phytogel and grown in a controlled environment. Five to seven days after planting, morphological parameters were measured to evaluate radiation-induced damage in the seedlings. After exposure to single types of charged particles, as well as to simulated GCR, the hydrated Arabidopsis seeds showed dose- and quality-dependent responses, with heavier ions causing more severe defects. Seeds exposed to simulated GCR (dry seeds) and SPE (hydrated seeds) had significant, although much less damage than seeds exposed to heavier and higher linear energy transfer (LET) particles. In general, the extent of damage depends on the seed type.

NASA's Ground-Based Microgravity Simulation Facility.

Since opportunities to conduct experiments in space are scarce, various microgravity simulators and analogs have been widely used in space biology ground studies. Even though microgravity simulators do not produce all of the biological effects observed in the true microgravity environment, they provide alternative test platforms that are effective, affordable, and readily available to facilitate microgravity research. The Microgravity Simulation Support Facility (MSSF) at the National Aeronautics and Space Administration (NASA) John F. Kennedy Space Center (KSC) has been established for conducting short duration experiments, typically less than 1 month, utilizing a variety of microgravity simulation devices for research at different gravity levels. The simulators include, but are not limited to, 2D Clinostats, 3D Clinostats, Random Positioning Machines, and Rotating Wall Vessels. In this chapter, we will discuss current MSSF capabilities, development concepts, and the physical characteristics of these microgravity simulators.

Trauma, process and representation.

The concept of Trauma in psychoanalysis has suffered from overuse and inconsistent use. A review of Freud's writings beginning with the Project indicates that from the perspective of the impact upon psychic processes, Freud held a more consistent view of the concept that, if recognized, can help avoid the often fruitless etiological debates of internal vs. external cause, intrinsic (drive) vs. extrinsic (reality) factors, etc. What is more helpful from a clinical perspective, is to view the various challenges that a given set of potentially trauma-inducing circumstances might pose for an individual, consider each individual's highly subjective mode of experiencing and responding to those challenges and take into account the supports offered in any instance by the specific familial, social or cultural surround. Each set of experiences that will be qualified as 'trauma' that any of us undergoes will to some extent be understood and integrated into our particular subjectivities according to our unique, subjective organizations of self, understandings of and position in the world.

FURTHER THOUGHTS ON TRAUMA, PROCESS AND REPRESENTATION.

Beginning with Freud, throughout his work and in most if not all psychoanalytic formulations, the concept of trauma has been associated with the disruptive effects of excess excitation on psychic regulatory processes and psychic development. Foremost among these are the capacities for emotional containment and representation. The restoration, strengthening or acquisition for the first time of these capacities can take place intersubjectively in a successful analytic therapy and lies at the heart of the therapeutic action.

Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent.

Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.

Hardware Validation of the Advanced Plant Habitat on ISS: Canopy Photosynthesis in Reduced Gravity.

The Advanced Plant Habitat (APH) is the largest research plant growth facility deployed on the International Space Station (ISS). APH is a fully enclosed, closed-loop plant life support system with an environmentally controlled growth chamber designed for conducting both fundamental and applied plant research during experiments extending as long as 135 days. APH was delivered to the ISS in parts aboard two commercial resupply missions: OA-7 in April 2017 and SpaceX-11 in June 2017, and was assembled and installed in the Japanese Experiment Module Kibo in November 2018. We report here on a 7-week-long hardware validation test that utilized a root module planted with both Arabidopsis (cv. Col 0) and wheat (cv. Apogee) plants. The validation test examined the APH's ability to control light intensity, spectral quality, humidity, CO2 concentration, photoperiod, temperature, and root zone moisture using commanding from ground facilities at the Kennedy Space Center (KSC). The test also demonstrated the execution of programmed experiment profiles that scheduled: (1) changes in environmental combinations (e.g., a daily photoperiod at constant relative humidity), (2) predetermined photographic events using the three APH cameras [overhead, sideview, and sideview near-infrared (NIR)], and (3) execution of experimental sequences during the life cycle of a crop (e.g., measure photosynthetic CO2 drawdown experiments). Arabidopsis and wheat were grown in microgravity to demonstrate crew procedures, planting protocols and watering schemes within APH. The ability of APH to contain plant debris was assessed during the harvest of mature Arabidopsis plants. Wheat provided a large evaporative load that tested root zone moisture control and the recovery of transpired water by condensation. The wheat canopy was also used to validate the ability of APH to measure gas exchange of plants from non-invasive gas exchange measurements (i.e., canopy photosynthesis and respiration). These features were evaluated by executing experiment profiles that utilized the CO2 drawdown technique to measure daily rates of canopy photosynthesis and dark-period CO2 increase for respiration. This hardware validation test confirmed that APH can measure fundamental plant responses to spaceflight conditions.

Reflections on Therapeutic Action and the Origins of Psychic Life.

Psychoanalysis has seen a shift in emphasis regarding therapeutic action and technique. A predominant focus on the uncovering or reintegrating of repressed, disguised, or split-off contents has moved to include the intersubjective creation, development, and strengthening of psychic processes and capabilities. The analyst's role in this process has been analogized to that of the primary maternal object in the origins of psychic life. This metaphor illuminates the movement from unrepresented to represented psychic states in treatment, as seen in a clinical example from the analysis of a particularly withdrawn young adult.

The compulsion to repeat: An introduction.

The Repetition Compulsion has been the source of much controversy and perplexity. From it's clinical introduction in 1914 in Remembering, Repeating and Working Through to it's metapsychological elaboration in 1920 in Beyond the Pleasure Principle, it has occupied a central position in Freud's thinking. Especially in regard to his later work, it can be seen to be intrinsic to his final dual instinct theory, his theories of the Death Instinct, trauma, memory, binding and action and to the clinical challenges and theoretical changes that led to the formulation of his second topography. This paper will trace the evolution of the concept in Freud and in certain post-Freudian authors, especially Edward Bibring, Winnicott and Scarfone.

Rapid Morphological and Cytoskeletal Response to Microgravity in Human Primary Macrophages.

The FLUMIAS (Fluorescence-Microscopic Analyses System for Life-Cell-Imaging in Space) confocal laser spinning disk fluorescence microscope represents a new imaging capability for live cell imaging experiments on suborbital ballistic rocket missions. During the second pioneer mission of this microscope system on the TEXUS-54 suborbital rocket flight, we developed and performed a live imaging experiment with primary human macrophages. We simultaneously imaged four different cellular structures (nucleus, cytoplasm, lysosomes, actin cytoskeleton) by using four different live cell dyes (Nuclear Violet, Calcein, LysoBrite, SiR-actin) and laser wavelengths (405, 488, 561, and 642 nm), and investigated the cellular morphology in microgravity (10-4 to 10-5 g) over a period of about six minutes compared to 1 g controls. For live imaging of the cytoskeleton during spaceflight, we combined confocal laser microscopy with the SiR-actin probe, a fluorogenic silicon-rhodamine (SiR) conjugated jasplakinolide probe that binds to F-actin and displays minimal toxicity. We determined changes in 3D cell volume and surface, nuclear volume and in the actin cytoskeleton, which responded rapidly to the microgravity environment with a significant reduction of SiR-actin fluorescence after 4-19 s microgravity, and adapted subsequently until 126-151 s microgravity. We conclude that microgravity induces geometric cellular changes and rapid response and adaptation of the potential gravity-transducing cytoskeleton in primary human macrophages.

Trauma, proceso y representación / Traumatisme, processus et représentation / Trauma, process and representation

El autor reflexiona desde una perspectiva contemporánea sobre el concepto de trauma y su relación con los procesos de representación. Partiendo de una dicotomía en la que todo se centraría en el conflicto intrapsíquico o bien al contrario solamente en las vivencias externas que interfieren en el desarrollo, se busca una posición sintética en que los aspectos traumáticos puedan irse integrando. Teniendo en cuenta lo traumático como algo que no solo depende de la intensidad del trauma, sino también de la capacidad de los objetos circundantes para ayudar a tramitarlo, se reflexiona sobre la actitud técnica más propicia en el analista: se da una especial importancia a su implicación en las construcciones, así como la trascendencia de poderse involucrar en la relación con los pacientes. Se trae el caso de un paciente con la historia de unos padres divorciados poco implicados en su crianza, y se relata cómo el involucrarse el analista en sus planes de veraneo supone un poderlo despertar de un cierto letargo. (AU)

The author articulates a contemporary perspective on the concept of trauma and its relationship to the processes of representation. Shifting from a dichotomy where the entire focus is on intrapsychic conflict, versus a focus only on the external experiences that interfere with development, a synthetic position, more integrative of traumatic aspects, is sought. Considering the traumatic as something that not only depends upon the intensity of the trauma, but also upon the capacity of the surrounding objects in helping to process it, the author reflects upon the most conducive technical attitude, according particular importance to the analyst’s involvement in constructions, as well as the vital importance of the ability to engage in their relationship with patients. Case material is presented, detailing a patient with divorced parents who had little involvement in his upbringing, and how the analyst becoming involved in his summer plans brought about a certain awakening from lethargy within him. (AU)

Partant d’une perspective contemporaine, l’auteur réfléchit sur le concept de traumatisme et sa relation avec les processus de représentation. A partir d’une dichotomie où tout se centrerait sur le conflit intrapsychique ou bien au contraire seulement sur les vécus externes qui interfèrent dans le développement, il cherche une position synthétique dans laquelle les aspects traumatiques peuvent peu à peu s’intégrer. Considérant ce qui est traumatique comme quelque chose qui ne dépend pas seulement de l’intensité du traumatisme mais également de la capacité des objets environnants pour aider à le traiter, il réfléchit sur l’attitude technique plus propice chez l’analyste: il attribue une importance spéciale à son implication dans les constructions, ainsi qu’à la transcendance de pouvoir s’engager dans la relation avec les patients. Il apporte le cas d’un patient avec une histoire de parents divorcés peu impliqués dans son éducation, et il raconte comment l’implication de l’analyste dans ses projets d’été suppose pouvoir le réveiller d’une certaine léthargie. (AU)