Evaluation of Rambouillet, Polypay, and Romanov-White Dorper × Rambouillet ewes mated to terminal sires in an extensive rangeland production system: Lamb production.

Ewe productivity (i.e., total number or weight of lambs weaned per breeding ewe) is a key indicator of lamb production efficiency. This study compared various measures of ewe productivity and ewe and lamb performance among ewes of 3 breed types mated to rams of 4 terminal-sire breed types in an extensive rangeland production system. Purebred Rambouillet ( = 212), purebred Polypay ( = 236), and crossbred Romanov-White Dorper × Rambouillet (RW-RA; = 231) ewes were produced from locally adapted Polypay and Rambouillet ewes and then annually mated to Columbia, Suffolk, Columbia × Suffolk, or Suffolk × Columbia sires for up to 4 yr, beginning at 1 yr of age. The cumulative number and weight of lambs weaned through 4 yr were greater for RW-RA (5.9 lambs and 153 kg, respectively) and Polypay ewes (4.9 lambs and 123 kg, respectively) than for Rambouillet ewes (2.9 lambs and 99 kg, respectively) and also were greater for RW-RA ewes than for Polypay ewes (all < 0.001). Greater productivity of RW-RA and Polypay ewes, compared with Rambouillet ewes, was driven by greater lambing rates (ewes lambing per ewe exposed) as ewe lambs (87 and 77 vs. 31%, respectively; < 0.001) and greater ( < 0.001) litter sizes as ewe lambs (1.3, 1.3, and 1.0, respectively) and adult ewes (2.1, 2.0, and 1.6, respectively). The RW-RA ewes also had greater longevity ( < 0.01) and cumulative lambing rates ( < 0.001) than Polypay and Rambouillet ewes. Lamb BW at birth and weaning in adult ewes favored less-prolific Rambouillet ewes ( < 0.001), but after adjustment for type of birth and rearing and weaning age, differences in weaning BW among ewe breed types were small and not significant ( = 0.08). Effects of sire breed type on measures of cumulative ewe productivity were not significant ( > 0.74), but Suffolk-sired lambs had the heaviest adjusted birth weights ( = 0.01) and Columbia-sired lambs tended to have the lightest adjusted weaning weights ( = 0.12). Combined effects of heterosis and additive breed effects were associated with greater lambing rates in ewe lambs, larger litters at all ages, and substantially greater number and weight of lambs weaned for Polypay and RW-RA ewes than for Rambouillet ewes.

Responses of pregnant ewes and young lambs to ovalbumin immunization, antiovalbumin antibody transfer to lambs, and temporal changes in antiovalbumin antibody.

Factors affecting the decay of maternally derived IgG and ability of neonatal lambs to produce protective amounts of their own IgG are not well understood. Thus, we conducted 3 experiments to quantify the 1) response of pregnant ewes to ovalbumin immunization, 2) antiovalbumin antibody (OV-IgG) transfer to lambs, 3) changes over time in OV-IgG in lambs, and 4) response of young lambs to ovalbumin immunization. In Exp. 1, ewes (n = 10/group) either received control (adjuvant + saline) or ovalbumin (ovalbumin + adjuvant + saline) injections at ≈ 42 and 14 d prepartum. Ovalbumin increased (P < 0.001) ewe serum and colostrum OV-IgG. Serum OV-IgG was greater (P < 0.0001) in lambs from ovalbumin-treated than in lambs from control ewes. In Exp. 2, lambs (n = 20/group), which were from ewes that had received ovalbumin prepartum, were given either control or ovalbumin injections on d 1 and 15 of age. From d 1 to 15, maternally derived OV-IgG was less (P < 0.04) in ovalbumin-treated than in control lambs. After d 15, OV-IgG was greater (P < 0.001) in ovalbumin-treated than in control lambs. In Exp. 3, lambs (n = 20/group), which were from ewes naïve to ovalbumin, received 1 of 4 treatments: 1) d-1 + d-15 control injections; 2) d-1 + d-15 ovalbumin; 3) d-28 + d-42 control; and 4) d-28 + d-42 ovalbumin. In d-1 + d-15 ovalbumin lambs, OV-IgG increased (P < 0.001) from d 7 to 21 after treatment and then decreased (P < 0.004) after d 28. In d-28 + d-42 ovalbumin lambs, OV-IgG increased (P < 0.001) steadily until d 21 after treatment and then stabilized after d 21. At ≈ 159 d of age, lambs in each group received injections consistent with their original type. After the d-159 treatment, ovalbumin injection increased (P < 0.0001) OV-IgG, and the injection type × time interaction was significant (P < 0.0001). In d-28 + d-42 ovalbumin lambs, OV-IgG just before the d-159 injections was greater (P < 0.006) than that in the other groups. In this study, late pregnant ewes produced OV-IgG after ovalbumin injections and then transferred OV-IgG to lambs via colostrum. Ovalbumin treatment of young lambs reduced circulating maternally derived OV-IgG, but it also induced an immune response in the lambs. Overall, our results support recommendations to vaccinate ewes against common pathogens during late pregnancy and to ensure that lambs receive adequate colostrum soon after birth.

Mutations in Ovis aries TMEM154 are associated with lower small ruminant lentivirus proviral concentration in one sheep flock.

Small ruminant lentivirus (SRLV), also called ovine progressive pneumonia virus or maedi-visna, is present in 24% of US sheep. Like human immunodeficiency virus, SRLV is a macrophage-tropic lentivirus that causes lifelong infection. The production impacts from SRLV are due to a range of disease symptoms, including pneumonia, arthritis, mastitis, body condition wasting and encephalitis. There is no cure and no effective vaccine for preventing SRLV infection. However, breed differences in prevalence and proviral concentration indicate a genetic basis for susceptibility to SRLV. Animals with high blood proviral concentration show increased tissue lesion severity, so proviral concentration represents a live animal test for control post-infection in terms of proviral replication and disease severity. Recently, it was found that sheep with two copies of TMEM154 haplotype 1 (encoding lysine at position 35) had lower odds of SRLV infection. In this study, we examined the relationship between SRLV control post-infection and variants in two genes, TMEM154 and CCR5, in four flocks containing 1403 SRLV-positive sheep. We found two copies of TMEM154 haplotype 1 were associated with lower SRLV proviral concentration in one flock (P < 0.02). This identified the same favorable diplotype for SRLV control post-infection as for odds of infection. However, frequencies of haplotypes 2 and 3 were too low in the other three flocks to test. The CCR5 promoter deletion did not have consistent association with SRLV proviral concentration. Future work in flocks with more balanced allele frequencies is needed to confirm or refute TMEM154 association with control of SRLV post-infection.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: VIII. Quality measures of lamb longissimus dorsi.

Quality measures of lamb longissimus dorsi were evaluated in 514 crossbred wether lambs to assess sire breed differences. Wethers were produced over 3 yr from single-sire matings of 22 Columbia, 22 U.S. Meat Animal Research Center (USMARC)-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were reared to weaning in an extensive western rangeland production system and finished in a feedlot on a high-energy finishing diet. One of three harvest groups were randomly assigned to each lamb, and lambs were transported to The Ohio State University abattoir when the mean BW of wethers remaining in the feedlot reached 54.4, 61.2, or 68.0 kg. After harvest, subjective lean quality scores were assigned and LM pH (immediately after and 24 h after harvest), color (quantified as Minolta L*, a*, and b*), intramuscular fat (IMF), cooking loss percentage, and Warner-Bratzler shear force (WBSF) were determined. Statistical models included fixed effects of sire breed, year of birth, and harvest group and random effects of sire (nested within sire breed and year) and maternal grandsire. Year and harvest group were significant (P < 0.04) for all traits, except harvest group was not for WBSF and lean quality (P > 0.28). At comparable numbers of days on feed, Texel-sired wethers had the greatest (more desirable; P < 0.01) subjective lean quality scores while Composite- and Suffolk-sired lambs were intermediate and Columbia-sired lambs had the lowest (less desirable). Minolta L* values were greater (P < 0.02) for Texel- than Columbia-sired lambs, although this difference is not visually discernible by humans. No significant (P > 0.05) sire breed effects were detected for LM pH at or 24 h after harvest, Minolta a* and b*, IMF, percentage of cooking loss, and WBSF at comparable numbers of days on feed. At comparable chilled carcass weight, significant (P < 0.01) sire breed effects were detected for subjective lean quality score. Texel-sired lambs had greater scores than Columbia- and Suffolk-sired lambs, but Composite-sired lambs did not differ from lambs sired by the other sire breeds. Sire breed effects were not detected (P > 0.15) for LM pH at or 24 h after harvest, Minolta L*, a*, and b*, cooking loss percentage, IMF, and WBSF at comparable chilled carcass weight. Therefore, results of the present study indicated that the 4 sire breeds produced crossbred lamb carcasses that had similar and desirable LM quality measurements, meeting consumer expectations.

Evaluation of Columbia, USMARC Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: VII. Accuracy of ultrasound predictors and their association with carcass weight, yield, and value.

Use of lamb BW or chilled carcass weights (CCW), live-animal ultrasound or direct carcass measurements of backfat thickness (BF; mm) and LM area (LMA; cm(2)), and carcass body wall thickness (BWall; mm) to predict carcass yield and value was evaluated using 512 crossbred lambs produced over 3 yr by mating Columbia, U.S. Meat Animal Research Center Composite, Suffolk, and Texel rams to adult Rambouillet ewes. Lambs were harvested at 3 BW endpoints within each year. The predictive value of 3 to 5 additional linear measurements of live-animal or carcass size and shape was also evaluated. Residual correlations (adjusted for effects of year, breed, and harvest group) between ultrasound and direct measurements were 0.69 for BF and 0.65 for LMA. Increasing ultrasound or carcass LMA had positive effects (P < 0.001) on yield of chilled carcass (i.e., on dressing percentage) and, at comparable CCW, on weight of high-value cuts (rack, loin, leg, and sirloin) before trimming (HVW), weight of trimmed high-value cuts (trimmed rack and loin and trimmed boneless leg and sirloin; TrHVW), and carcass value before (CVal) and after (TrCVal) trimming of high-value cuts. By contrast, ultrasound and direct measures of BF had positive effects on yields of CCW and on HVW and CVal but large negative effects on TrHVW and TrCVal. After adjusting for BW at scanning, increases of 1 mm in ultrasound BF or 1 cm(2) in ultrasound LMA were associated with changes of US$-0.32 (P < 0.10) and $1.62 (P < 0.001), respectively, in TrCVal. Carcass BWall was generally superior to carcass BF as a predictor of TrHVW and TrCVal. Carcass LMA was superior to ultrasound LMA but carcass BF was inferior to ultrasound BF for prediction of carcass yield and value. Increasing LMA thus would be expected to improve carcass yield and value. Addition of linear measurements of live-animal or carcass size and shape to the prediction model reduced residual SD (RSD) for TrHVW and TrCVal by 0.4 to 2.2%, but subsequent removal of ultrasound or direct measures of BF and LMA from the prediction model increased RSD by 7.4 to 12.2%. Measurements of CCW, LMA, BF, and BWall would thus be appropriate to support programs for value-based marketing of lamb carcasses and are superior to systems based only on measurements of size and shape in unribbed carcasses.

Evaluation of Columbia, U.S. Meat Animal Research Center Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: VI. Measurements of live-lamb and carcass shape and their relationship to carcass yield and value.

Linear measurements on live lambs and carcasses can be used to characterize sheep breeds and may have value for prediction of carcass yield and value. This study used 512 crossbred lambs produced over 3 yr by mating Columbia, U.S. Meat Animal Research Center (USMARC) Composite, Suffolk, and Texel rams to adult Rambouillet ewes to assess sire-breed differences in live-animal and carcass shape and to evaluate the value of shape measurements as predictors of chilled carcass weight (CCW), weight of high-value cuts (rack, loin, leg, and sirloin; HVW), weight of trimmed high-value cuts (trimmed rack and loin and trimmed, boneless leg and sirloin; TrHVW), and estimated carcass value before (CVal) and after trimming of high-value cuts (TrCVal). Lambs were produced under extensive rangeland conditions, weaned at an average age of 132 d, fed a concentrate diet in a drylot, and harvested in each year in 3 groups at target mean BW of 54, 61, and 68 kg. Canonical discriminant analysis indicated that over 93% of variation among sire breeds was accounted for by the contrast between tall, long, less-thickly muscled breeds with greater BW and CCW (i.e., the Columbia and Suffolk) compared with shorter, more thickly muscled breeds with smaller BW and CCW. After correcting for effects of year, harvest group, sire breed, and shipping BW, linear measurements on live lambs contributed little to prediction of CCW. Similarly, after accounting for effects of CCW, linear measurements on live animals further reduced residual SD (RSD) of dependent variables by 0.2 to 5.7%, with generally positive effects of increasing live leg width and generally negative effects of increasing heart girth. Carcass measurements were somewhat more valuable as predictors of carcass merit. After fitting effects of CCW, additional consideration of carcass shape reduced RSD by 2.1, 3.6, 9.5, and 2.2% for HVW, TrHVW, CVal, and TrCVal, respectively. Effects of increasing carcass leg width were positive for HVW, TrHVW, and TrCVal. We also observed positive effects of increasing carcass length on TrCVal and negative effects of increasing cannon bone length on HVW and CVal. Increasing shoulder width had positive effects on CVal but negative effects on TrHVW. Differences in lamb and carcass shape were significantly associated with carcass yield and value, but the additional accuracy associated with use of these measurements was modest relative to that achieved from use of only shipping BW or CCW.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: V. Postweaning growth, feed intake, and feed efficiency.

This study was conducted to quantify differences in feed efficiency among 4 sire breeds using records from 1,109 crossbred lambs over 3 yr. Lambs were produced from mating Columbia, USMARC-Composite (Composite), Suffolk, and Texel rams to adult Rambouillet ewes. After weaning, lambs were finished on a high-energy diet in group-fed pens. Efficiency was assessed at 45 and 90 d on study, 50 and 60 kg of BW, and 6.6 and 9.1 mm of fat depth between the 12th and 13th ribs. Sire breed affected most of the traits that were measured. At 90 d, Suffolk-sired lambs had gained 13 to 19% more BW (P < 0.001), were 7 to 13% heavier (P < 0.001), and had consumed 4 to 11% more (P < 0.001) ME than the other breed crosses. However, at 90 d, G:F (59.9 vs. mean of 56.2 g BW gain/Mcal of ME; P < 0.01) and residual BW gain (RG; 0.94 vs. -0.33 kg BW; P < 0.05) were greater for Suffolk-sired lambs than for the other 3 sire breeds. Also, RG for Suffolk-sired lambs was greater (P < 0.01) at 6.6 and 9.1 mm of fat depth (0.62 vs. -0.24 kg and 0.87 vs. -0.33 kg of BW, respectively) and at 60 kg of BW (0.73 vs. 0.31 kg of BW) than for the other sire breeds. At 90 d, Columbia-sired lambs had the greatest (P < 0.01) residual feed intake (RFI; 7.01 vs. mean of -2.33 Mcal ME for the 3 other sire breeds). At 60 kg of BW, Texel- and Composite- (-2.34 Mcal ME, combined) had more favorable (P < 0.01) RFI than Columbia-sired lambs (6.42 Mcal ME), and Suffolk-sired lambs (0.49 Mcal ME) were intermediate and not different from the other 3 sire breeds. At 6.6 mm of fat depth, Columbia- and Suffolk- (3.30 Mcal ME, combined) had greater (P < 0.001) RFI than Texel-sired lambs (-4.23 Mcal ME); at 9.1 mm of fat depth, Columbia-sired lambs had the greatest (P < 0.05) RFI (7.35 vs. -2.11 Mcal ME for the other breeds combined). In summary, except for ME intake at 90 d, Suffolk-sired lambs were equal or superior to Columbia-, Composite-, and Texel-sired lambs for BW, BW gain, ME intake, G:F, RFI, and RG to 90 d, 60 kg of BW, and 9.1 mm of fat depth. Breed rankings were consistent across the study period for G:F and RG, but rankings changed over time for RFI.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: IV. Postfabrication carcass component weights.

Postfabrication carcass component weights of 517 crossbred wether lambs were analyzed to evaluate 4 terminal-sire breeds. Wethers were produced over 3 yr from single-sire matings of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were reared to weaning in an extensive western rangeland production system and finished in a feedlot on a high-energy finishing diet. When wethers reached a mean BW of 54.4, 61.2, or 68.0 kg, they were transported to The Ohio State University abattoir for harvest. After refrigeration for approximately 24 h, chilled carcass weight (CCW) was measured, carcasses were fabricated according to Style A of Institutional Meat Purchase Specifications, and postfabrication weights were recorded. At comparable numbers of days on feed, Suffolk-sired lambs had heavier (P < 0.04) neck, breast, shoulder, foreshank, rack, loin, leg, sirloin, roast-ready rack, trimmed loin, and boneless leg cuts than progeny of the other sire breeds. Boneless sirloins were heavier (P < 0.01) for Suffolk-sired than Composite-sired lambs but did not differ from those for Columbia- or Texel-sired lambs. Columbia- and Suffolk-sired lambs had heavier (P < 0.01) hindshanks than Texel-sired lambs. Suffolk-sired lambs had heavier (P < 0.01) high-value cuts (rack, loin, leg, and sirloin) and trimmed high-value cuts than progeny of the other sire breeds. Cutting loss (CCW - wholesale cut weights) and high-value trimming loss were greatest (P < 0.02) for Suffolk-sired lambs and least for Texel- and Composite-sired lambs. Sire breed did not affect (P > 0.06) flank weight. Data adjusted to comparable CCW reduced the number of significant sire-breed effects and changed sire-breed rankings of carcass component weights, for which sire breeds differed. After adjusting, Suffolk-sired lambs had lighter (P < 0.05) loins than Columbia- and Composite-sired lambs, Composite-sired lambs had heavier (P < 0.05) high-value cuts than Suffolk-sired lambs, and Suffolk- and Columbia-sired lambs had heavier (P < 0.05) necks than Texel-sired lambs. At predicted backfat thickness of 6.6 mm, Composite-sired lambs had a greater (P < 0.05) percentage of high-value cuts than Suffolk-sired lambs before but not after trimming. Producers can use these results to select terminal-sire breeds that will complement their production system and improve lamb value.

In vivo evaluation of the persistant and residual antimicrobial properties of three hand-scrub and hand-rub regimes in a simulated surgical environment.

BACKGROUND: The use of antimicrobial formulations for disinfecting hands prior to surgery has been shown to reduce the incidence of surgical site infections. Such formulations must demonstrate an immediate reduction of microbial flora on the hands that persists while the hands are gloved. AIM: This study compared persistent and residual antimicrobial effects of three simulated in-use surgical hand-cleansing procedures, one using a scrub followed by a hand rub with products containing chlorhexidine gluconate (CHG), and two using a scrub with a cleansing soap followed by a hand rub with one of two alcohol-based products. METHODS: The study was executed in two phases. In phase 1, persistent antimicrobial effects versus the resident microbial flora of volunteer subjects' hands were evaluated. In phase 2, the residual antimicrobial effects were challenged with the application of Staphylococcus aureus (ATCC 6538) on to the hands of volunteer subjects. FINDINGS: Phase 1 testing showed that significantly greater reductions of normal flora (P ≤ 0.00) persisted with a scrub with the CHG product followed by alcohol/CHG hand rub, than were achieved by scrubs with soap followed by application of either of the other hand rubs. Through all protocols of phase 2, the CHG scrub and alcohol/CHG hand rub produced significantly greater reductions of the S. aureus population (P ≤ 0.00) than did a soap scrub in combination with the other two hand rubs. CONCLUSION: The combination of a scrub and rub with products containing CHG and alcohol was shown to reduce significantly and persistently both the resident flora and contaminating transient bacteria on skin beneath surgical gloves.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: I. Ewe productivity and crossbred lamb survival and preweaning growth.

A 3-yr study was conducted to comprehensively evaluate Columbia, Suffolk, USMARC-Composite (Composite), and Texel breeds as terminal sires in an extensive rangeland production system. The objective was to estimate breed-of-ram effects on ewe fertility, prolificacy, and dystocia, and sire breed effects on lamb survival and growth until weaning at approximately 132 d of age. Data were from 22 Columbia, 22 Composite, 21 Suffolk, and 17 Texel rams with 957 exposures to 574 adult Rambouillet ewes (3- to 7-yr-old at lambing), 908 lambings, and 1,834 lambs. Ram breed did not affect ewe fertility (mean = 94.9%; p = 0.73), total number born per ewe lambing (mean = 2.02 lambs; p = 0.20), number born alive per ewe lambing (mean = 1.90 lambs; p = 0.24), or number weaned per ewe lambing (mean = 1.45 lambs, p = 0.94). Dystocia rates were different (p = 0.01) for ewes mated to Columbia (12.2%), Composite (13.5%), Suffolk (25.7%), and Texel rams (31.9%) during 1 yr of the study, but differences among ram breeds were not repeatable (p ≥ 0.38) during the other 2 yr. Suffolk-sired lambs were heavier (p ≥ 0.02) at birth (5.5 kg) and weaning (40.3 kg) than lambs sired by the other breeds, which did not differ (p ≥ 0.34) for birth weight (mean = 5.3 kg). Texel-sired lambs (37.4 kg) were lighter (p ≥ 0.02) at weaning than Columbia- (38.8 kg) and Composite-sired (38.4 kg) lambs, which did not differ (p = 0.40) for weaning weight. Sire breed effect approached significance (p = 0.06) for lamb survival to weaning; estimated survival probabilities were 0.87 (Columbia), 0.89 (Composite), 0.93 (Suffolk), and 0.86 (Texel) for lambs reared by their birth dam. Interaction between sire breeds and birth weight affected (p < 0.001) lamb survival and revealed that lightweight Columbia- and Suffolk-sired lambs had a greater risk of death than lightweight lambs sired by Composite and Texel rams, but risk of death did not increase substantially for heavyweight lambs from any of the breeds. When mated to adult Rambouillet ewes in an extensive rangeland production system, the use of Suffolk rams is warranted to improve preweaning growth of market lambs and is not predicted to affect ewe fertility, ewe prolificacy, dystocia, or lamb survival compared with the other sire breeds we tested.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: III. Prefabrication carcass traits and organ weights.

To evaluate terminal-sire breeds, harvest BW, prefabrication carcass measurements, and organ weights were evaluated over 3 yr for 518 crossbred wether lambs (ovis aries). Lambs were produced by single-sire matings of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, and 17 Texel rams to adult Rambouillet ewes. Lambs were raised to weaning under an extensive western rangeland production system and finished in a feedlot on a high-energy finishing diet. Wethers were randomly assigned to be harvested at an average BW of 54.4, 61.2, or 68.0 kg and then transported to The Ohio State University abattoir for harvest. Wether BW was recorded before transport (off-test BW) and before harvest. Prefabrication carcass measurements and organ weights were recorded either after harvest or after an approximate 24-h chill. At comparable numbers of days on feed, Suffolk-sired lambs had heavier (P < 0.01) off-test BW, harvest BW, HCW, chilled carcass weight (CCW), and kidney weights than lambs sired by the other breeds. Suffolk-sired lambs had more (P < 0.01) kidney-pelvic fat than did Columbia-sired lambs; Composite- and Texel-sired lambs were intermediate and did not differ (P > 0.06) from the other crossbred lambs. Texel- and suffolk-sired lambs had larger lm area and greater conformation scores than Columbia-sired lambs (P < 0.03). Texel-sired lambs had greater (P < 0.01) body wall thickness, quality grades, and leg scores than Columbia-sired lambs. Composite- and Suffolk-sired lambs did not differ from each other or from lambs sired by any other breed for body wall thickness (P > 0.18) and were intermediate for quality grades and leg scores. Sire breed did not affect (P > 0.05) shipping shrink, dressing percentage, pelt weight, liver weight, and fat depth. Adjusting data to a comparable off-test BW or CCW modified sire breed rankings for some measurements. Texel-sired lambs were equal or superior to lambs sired by other breeds for HCW, CCW, dressing percentage, pelt weight, LM area, quality grade, and leg and conformation scores. However, Texel-sired lambs also had values for kidney-pelvic fat weight, fat depth, and body wall thickness that were equal to or greater than those of lambs sired by the other breeds, indicating increased fatness at comparable BW. Producers can use these results to select terminal-sire sheep breeds that will complement their production system and improve market lamb value.

Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: II. Postweaning growth and ultrasonic measures of composition for lambs fed a high-energy feedlot diet.

Over 3 yr, postweaning growth patterns and changes in ultrasonic measurements of fat depth (FD) and LM area were assessed for 1,049 crossbred ewe and wether lambs (Ovis aries) produced by mating adult Rambouillet ewes to 1 of 22 Columbia, 22 USMARC-Composite (Composite), 21 Suffolk, or 17 Texel rams and raised to weaning under extensive Western rangeland conditions. After weaning, lambs were transitioned to a high-energy finishing diet in a feedlot, weighed weekly for 13 to 16 wk, and scanned using ultrasound at 2-wk intervals to estimate FD and LM area between the 12th and 13th ribs. Lambs sired by Suffolk rams were 5 to 12% heavier (P < 0.05) at start of test, grew 14 to 22% faster (P < 0.001), and were correspondingly 7 to 14% heavier (P < 0.001) after 90 d on test than lambs sired by rams of the other 3 breeds. At this time, lambs sired by Texel rams were 5% lighter (P < 0.01) than Columbia- or Composite-sired lambs, which did not differ (P > 0.20). Columbia-sired lambs had less FD (8.9 mm) but smaller LM areas (15.6 cm(2)) at 90 d on test (both P < 0.01) than lambs sired by rams of the other breeds (average of 9.8 mm for FD and 16.6 cm(2) for LM area), which did not differ for either measurement at this time. At 60 kg, predicted FD differed among sire breeds (P < 0.001) and were similar for Suffolk- (7.6 mm) and Columbia-sired (7.9 mm) lambs, intermediate for Composite-sired lambs (9.1 mm), and largest for Texel-sired (10.1 mm) lambs. At a BW of 60 kg, LM area differed between all pairs of sire breeds (P < 0.05), and was largest for Texel-sired lambs (16.7 cm(2)), followed by Composite-, Suffolk-, and Columbia-sired lambs (15.7, 14.8, and 14.5 cm(2), respectively). At a predicted FD of 9.1 mm (the boundary between U.S. Yield Grades 3 and 4), the predicted LM area for Suffolk-sired lambs (16.2 cm(2)) was similar (P > 0.10) to that of Texel-sired lambs (16.0 cm(2)) and superior (P < 0.01) to that of Composite- (15.7 cm(2)) and Columbia-sired lambs (15.4 cm(2)). Lambs sired by Suffolk rams were thus equal or superior to lambs sired by the other 3 breeds in growth, FD, and LM area at comparable ages, fatness at 60 kg, and LM area at a FD of 9.1 mm, but inferior in LM area to Texel- and Composite-sired lambs at comparable BW.

Sodium chlorate reduces the presence of Escherichia coli in feces of lambs and ewes managed in shed-lambing systems.

Our objective was to establish doses of orally administered NaClO(3) that reduced the presence of generic Escherichia coli in intestines of ewes and neonatal lambs managed in a shed-lambing system. Neonatal lambs (n = 32; age = 7.1 ± 1.2 d; BW = 6.8 ± 1.0 kg) and yearling ewes (n = 44; BW = 74.8 ± 5.6 kg) were used in 2 experiments. In both experiments, lambs and ewes were randomly assigned to 1 of 4 groups, and groups were randomly assigned to 1 of 4 treatments. In Exp. 1, neonatal lambs were given single, aqueous, oral doses of saline (control; NaCl, 30 mg·kg of BW(-1)) or 30, 60, or 90 mg of NaClO(3)·kg(-1) of BW. At 25.9 ± 1.3 h after treatment, lambs were euthanized, and intestinal contents were collected aseptically. In Exp. 2, ewes were given single, aqueous, oral doses of saline (NaCl, 150 mg·kg of BW(-1)) or 150, 300, or 450 mg of NaClO(3)·kg(-1) of BW. At 24.0 ± 0.8 h after treatment, fecal samples were collected aseptically from the rectum of each ewe. For both experiments, generic E. coli were enumerated from intestinal contents and feces within 4 to 12 h after collection. In Exp. 1, the effect (P = 0.08) of NaClO(3) on the presence of generic E. coli in colon contents was dose-dependent. This effect was linear (P < 0.01) and negative, which indicated that as NaClO(3) dose increased, generic E. coli that could be isolated from colon contents decreased. Specifically, lambs dosed with 60 and 90 mg of NaClO(3)·kg(-1) of BW had fewer E. coli cfu·g(-1) of content than control lambs (P < 0.06). Lambs dosed with 90 mg of NaClO(3)·kg(-1) of BW had fewer E. coli cfu·g(-1) of content than lambs dosed with 30 mg of NaClO(3)·kg(-1) of BW (P = 0.09). Sodium chlorate dose did not influence (P = 0.58) the presence of generic E. coli in contents collected from the cecum. In Exp. 2, the effect (P < 0.0001) of NaClO(3) on the presence of E. coli in fecal contents from ewes was dose-dependent. This effect was quadratic (P < 0.0001) and negative; ewes dosed with 150, 300, and 450 mg of NaClO(3)·kg(-1) of BW had fewer E. coli cfu·g(-1) of feces than control ewes. No differences in E. coli cfu·g(-1) of feces were detected between NaClO(3) treatments (P = 0.88 to 0.97). Based on these results, a single oral dose of at least 60 and 150 mg of NaClO(3)·kg(-1) of BW in neonatal lambs and yearling ewes, respectively, significantly decreased the presence of generic E. coli in contents from the lower intestine.

Common promoter deletion is associated with 3.9-fold differential transcription of ovine CCR5 and reduced proviral level of ovine progressive pneumonia virus.

Chemokine (C-C motif) Receptor 5 (CCR5) is a chemokine receptor that regulates immune cell recruitment in inflammation and serves as a coreceptor for human immunodeficiency virus (HIV). A human CCR5 coding deletion (termed delta-32) results in strong resistance to HIV infection, and sequence variants in CCR5 regulatory regions have been implicated in delayed progression to acquired immune deficiency syndrome. Both ovine progressive pneumonia virus (OPPV), also known as maedi-visna, and HIV are macrophage-tropic lentiviruses, have similar genomic structures, and cause lifelong persistent host infection, suggesting CCR5 may have a role in regulating OPPV provirus levels. Therefore, the ovine CCR5 genomic sequence was determined, and sequence variants were obtained from the open reading frame and surrounding regulatory sites. One CCR5 variant contained a 4-base deletion within a binding site for octamer transcription factors in the promoter region. A test for differential transcription from each allele in heterozygous animals showed a 3.9-fold transcription difference (P < 0.0001). OPPV proviral levels were also measured in 351 naturally exposed Rambouillet, Polypay and Columbia sheep. Deletion homozygotes showed reduced OPPV proviral levels among these animals (P < 0.01). The association of this CCR5 promoter deletion with OPPV levels will need to be validated in additional populations before the deletion can be recommended for widespread use in marker-assisted selection. However, because of the large impact on transcription and because CCR5 has roles in inflammation, recruitment of effector cells, and cell-mediated immunity, this deletion may play a role in the control of infections of many diverse pathogens of sheep.

B-mode, real-time ultrasound for estimating carcass measures in live sheep: accuracy of ultrasound measures and their relationships with carcass yield and value.

Accuracy and repeatability of live-animal ultrasound measures, and the relationships of these measures with subprimal yields and carcass value, were investigated using data from 172 wethers. Wethers were F(1) progeny from the mating of 4 terminal sire breeds to Rambouillet ewes and were finished in a feedlot to a mean BW of 62.9 kg (SD = 9.5 kg). Before transport to slaughter, LM area, LM depth, and backfat thickness were measured from transverse ultrasound images taken between the 12th and 13th ribs. After slaughter, these measures were taken on each carcass. Carcasses were fabricated into subprimal cuts, and weights were recorded. Ultrasound accuracy and repeatability were assessed using bias, SE of prediction, SE of repeatability, and simple correlations. Relationships among ultrasound and carcass measures, and between these measures and carcass yield and value, were evaluated using residual correlations and linear prediction models. Ultrasound bias approached 0 for LM area, and backfat thickness was overestimated by only 0.69 mm. The SE of prediction and r were 1.55 cm(2) and 0.75 for LM area, and 1.4 mm and 0.81 for backfat thickness, respectively. The SE of repeatability was 1.31 cm(2) and 0.75 mm for LM area and backfat thickness, respectively. At a standardized BW and backfat thickness, wethers with larger LM area and LM depth yielded larger and more valuable carcasses, and these relationships were detectable with ultrasound. For each SD increase in carcass LM area, dressing percentage increased 1.57 percentage points, gross carcass value increased US$5.12, and boxed carcass value increased US$6.84 (P < 0.001). For each SD increase in ultrasound LM area, dressing percentage increased 0.95 percentage points, gross carcass value increased US$3.15, and boxed carcass value increased US$3.86 (P < 0.001). When LM area effects were adjusted for carcass weight, the response in boxed carcass value attributed to disproportionate increases in high-value subprimal cut weights was small. Associations of dressing percentage and carcass value with ultrasound and carcass LM depth were significant (P < 0.01) but smaller than corresponding associations with LM area. These data indicate biological and economical incentives for increasing LM area in wethers, and live-animal ultrasound can provide reliable estimates of carcass measures. These results are applicable to terminal sire breeders and producers who market sheep using carcass-merit pricing systems.

Evaluation of three-ram cohort serving capacity tests as a substitute for individual serving capacity tests.

Alternatives to time-consuming, laborious individual serving capacity tests (ISCT) are needed to classify ram sexual behavior. The objective of study 1 was to evaluate the relationship between the first 3-ram cohort test (COSCT) scores and the mean of 5 ISCT scores. The objective of study 2 was to determine whether 1 or 2 additional COSCT improved the ability to predict ISCT scores. For study 1, rams (n = 69) were assigned to either a COSCT given before or after 9 ISCT. For study 2, rams (n = 127) were given 3 COSCT before or after 6 ISCT. For repeated COSCT, rams were initially grouped at random and subsequently rerandomized so that each ram was grouped with at least 1 different ram for each test. For both studies, the number of ejaculations from COSCT was compared with the mean number of ejaculations across the second through sixth ISCT. A threshold between high- and low-performing rams was defined in each analysis as the mean ISCT scores of sexually active rams. Rams with a mean number of ejaculations in ISCT greater than the threshold were classified as having high sexual activity, whereas rams below the threshold were classified as having low sexual activity. Rams with no ejaculations in ISCT were classified as sexually inactive. Data from studies 1 and 2 were used to evaluate the relationship between the first COSCT and the mean of 5 ISCT scores. Data from multiple COSCT were fit to various models to determine whether the ability to predict ISCT scores was improved with 1 or 2 additional COSCT. The best model for ISCT and COSCT was a piecewise linear regression model. The first COSCT correctly identified all sexually inactive rams in both studies. The first COSCT, however, also classified 56% of low sexually active rams and 18% of high sexually active rams as inactive. Rams had a 71% probability of high sexual activity in ISCT if they were classified as sexually active in the first COSCT. We conclude that a single COSCT is a reliable, albeit more conservative, and efficient alternative to a series of ISCT for characterizing sexual activity of rams. Multiple COSCT can provide some protection against culling rams with high sexual activity (i.e., approximately 50% less with 2 additional COSCT) and still retain most of the efficiency compared with ISCT. It is important to use high-performance rams for breeding because they will approximately double the number of ewes bred and lambs sired compared with low-performance rams if a large number of ewes need to be serviced daily.

The influence of breeding intensity on above- and below-average sexual performance rams in single- and multiple-sire breeding environments.

Two studies were conducted to evaluate the relationship between serving capacity scores and breeding performance of rams. The first study was conducted to determine whether rams with above or below mean serving capacity scores could perform equally in greater and lesser breeding intensity, single-sire mating schemes. The second study was conducted to determine whether rams with above and below mean serving capacity scores could perform equally well when only one or two ewes were in estrus daily in a multiple-sire breeding scheme (two rams/pen). Rams (n=68) were ranked according to average number of ejaculations recorded in serving capacity tests. Sixteen rams with the greatest scores (above-average) and 16 rams with least scores (below-average) were identified for breeding. Half of above-average and half of below-average rams were used in the two studies. For study 1, each ram was individually introduced to 23 estrus-synchronized ewes for 9 d to simulate high breeding intensity. Rams were given a 5-d rest before they were individually introduced to 23-24 naturally cyclic ewes for 17 d (low breeding intensity). For study 2, 16 rams were paired across ram types, and each pair competed for 20 ewes for 18 d (8 pens). For study 1, ewe fertility (ewes lambing/ewes present at lambing) and number of lambs born were greater (P<0.001) for above-average (0.67+/-0.03 and 27.6+/-1.2, respectively) than for below-average rams (0.39+/-0.07 and 15.3+/-2.7) with greater breeding intensity. Ewe fertility and lambs born did not differ for above-average (0.91+/-0.03 and 37.8+/-1.9, respectively) and below-average rams (0.86+/-0.03 and 39.0+/-1.9) with less breeding intensity. For study 2, number of ewes lambing (99+/-8.0 compared with 72+/-13.6; P=0.12) and number of lambs sired (149+/-18.5 compared with 101+/-22.8; P=0.14) did not differ between above- and below-average rams, respectively, in direct competition. Sexual classifications based on serving capacity tests are related to breeding performance of rams in certain breeding environments. When breeding intensity is greater, above-average rams impregnate more ewes and sire more lambs than below-average rams. When only a small number of ewes are in estrus daily, below-average rams for serving capacity scores perform as well as above-average rams in multiple-sire and single-sire breeding environments. We suggest that above-average rams should be used to reduce number of rams required when breeding intensity is greater.

Intrauterine bacterial inoculation and level of dietary methionine alter amino acid metabolism in nulliparous yearling ewes.

Using an intrauterine bacterial inoculation method, our objective was to determine the effects of acute sepsis and level of dietary metabolizable Met (MM) on splanchnic metabolism of AA in ewes. Twenty-four nulliparous yearling Rambouillet-cross ewes (initial BW = 65.1 +/- 0.6 kg), surgically fitted with chronic-indwelling catheters in hepatic and portal veins, a mesenteric vein and artery, and the uterine lumen, were assigned to a 2 x 2 factorial arrangement of treatments. Factors were intrauterine bacterial inoculation (noninoculated vs. inoculated) and level of MM [low (2.28 g/d) vs. high (3.91 g/d)]. Beginning 12 h before sampling, inoculated and noninoculated ewes received 10-mL intrauterine infusions of Escherichia coli (9.69 x 10(11) cfu) + Arcanobacterium pyogenes (2.76 x 10(12) cfu) and of sterile saline, respectively. Uterine infection was induced in ewes that received intrauterine bacterial inoculations, but not in ewes infused with sterile saline. Bacterial inoculation resulted in increased hepatic release and plasma concentrations of aromatic AA used for acute-phase protein synthesis, increased hepatic removal and decreased plasma concentrations of AA used for glutathione synthesis, and decreased plasma concentrations of some gluconeogenic and acetogenic AA used for glucose recycling and anaerobic energy production, respectively (P < 0.05). In ewes fed high-MM diets, compared with low-MM diets, a consistent net hepatic uptake of Phe occurred throughout the sampling period, more Asp was released from the portal-drained viscera, and hepatic vein glucose concentrations were greater (P < 0.05). We conclude that Met seemed to be limiting in low-MM ewes, and as such, would continue to be limiting during sepsis. However, additional MM, in excess of the dietary requirement, would not necessarily result in a benefit to ewes experiencing acute sepsis.

Effect of early and late exposure to estrual ewes on ram sexual performance classifications.

This study was conducted to determine whether exposure of ram lambs to estrual ewes during their first autumn and again as adults just before serving capacity tests (SCT) affected the outcome of the sexual performance tests. Treatments were either early exposure of Polypay ram lambs (i.e., 7-8-mo-old rams with ewes for 17 d [n=30] or no early exposure [n=30]), and late exposure (i.e., 16-19-mo-old rams with estrual ewes for 3 d) or no exposure to estrual ewes in a 2x2 factorial arrangement. Three serving capacity tests were conducted immediately after the early exposure period for individual ram lambs that were exposed to ewes early. Three sham sexual performance tests (i.e., four ram lambs placed in test pens for 30-min without ewes) were conducted with ram lambs that were not exposed to ewes early. All rams were evaluated during nine 30-min serving capacity tests over a 2-mo period at 16-19 mo of age to determine sexual performance. Prior to serving capacity tests, one half of the rams from each early exposure treatment were exposed to estrual-induced ewes for 3 d. Specific sexual behaviors (e.g., sniffs, flehmens, foreleg kicks, vocalizations, mount attempts, mounts, and ejaculations) were recorded during serving capacity tests. Number of sniffs, flehmens, foreleg kicks, vocalizations, and mount attempts were summed without estimating the value of importance and analyzed as courtship behaviors. Sexual performance data were analyzed with Mixed model procedures for repeated measures. During serving capacity tests, the early exposed rams exhibited more courtships (40.3+/-8.0 versus 23.4+/-4.6; P<0.05; LSM+/-estimated SE), mounts (11.3+/-1.0 versus 7.7+/-0.9; P<0.01), and ejaculations (3.3+/-0.2 or 2.4+/-0.2; P<0.01) than rams not exposed to ewes as ram lambs, respectively. We conclude that early exposure of 7-8-mo-old ram lambs to estrual ewes improves sexual performance in serving capacity tests at 16-19 mo of age in most rams whereas, late exposure to estrual ewes for 3 d prior to serving capacity tests did not improve sexual performance scores.