RESUMEN
This study investigated the influence of the surface microporosity of beta-tri-calcium phosphate (ß-TCP) ceramics on the resorption capacity of osteoclasts. This was achieved by first compacting commercially available ß-TCP powder into disks that were sintered at various temperatures, thereby yielding different surface microporosities. Scanning electron microscopy (SEM) and subsequent image processing verified different degrees of surface microporosity on the disks. Rabbit osteoclasts in a bone marrow derived cell suspension were then seeded onto these disks and incubated for 48 h. Tartrate resistant acid phosphatase (TRAP) staining confirmed the presence of osteoclasts on all disks. Actin ring staining that detected actively resorbing OCs showed an inverse linear correlation between the number of actively resorbing osteoclasts (percentage of total OCs on the surfaces) with surface microporosity. These findings should be taken into consideration for the design and/or production of new ß-TCP bone graft substitutes.
Asunto(s)
Materiales Biocompatibles/farmacología , Resorción Ósea/patología , Cerámica/farmacología , Osteoclastos/efectos de los fármacos , Osteoclastos/patología , Fosfatasa Ácida/metabolismo , Actinas/metabolismo , Animales , Materiales Biocompatibles/química , Fosfatos de Calcio/farmacología , Recuento de Células , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Cerámica/química , Isoenzimas/metabolismo , Osteoclastos/enzimología , Osteoclastos/ultraestructura , Porosidad , Conejos , Coloración y Etiquetado , Fosfatasa Ácida Tartratorresistente , Difracción de Rayos XRESUMEN
UNLABELLED: Osteoporosis affects the craniofacial and oral structures and has been associated with periodontal bone loss, tooth loss and reduced jaw bone mass. OBJECTIVE: This study aimed to test the therapeutic efficacy of synthetic bone mineral (SBM) in minimizing alveolar bone loss induced by mineral deficiency in a rat model. SBM consists of a calcium carbonate apatite (similar to bone apatite) matrix incorporating magnesium, zinc, and fluoride ions. DESIGN: Thirty female Sprague Dawley rats (2 months old) were randomly distributed into 3 groups (10 rats per group): GA (control), on basic diet; GB, on mineral deficient (MD) diet; and GC, on MD+SBM. The rats were sacrificed after 3 months, the jawbones were isolated and the soft tissues removed. Bone density was determined using X-ray radiography (Faxitron); mandibular cortical width, panoramic mandibular index, and alveolar resorption degree (M/M ratio) using BioquantOsteo; and bone micro-architecture micro-computed tomography and scanning electron microscopy. RESULTS: Compared to control (GA), the rats on MD diet (GB) experienced significant mandibular bone loss while the rats on MD+SBM diet (GC) experienced significantly less bone loss compared to the GB group. CONCLUSION: SBM, administered orally, may have the potential as an osteoporosis therapeutic agent in minimizing or preventing alveolar bone loss induced by mineral deficiency.
