The significance and prognostic value of multifocal papillary thyroid carcinoma in children and adolescents.

INTRODUCTION: The prognostic value of multifocality in paediatric papillary thyroid carcinoma (PTC) patients remains a subject of debate. This study aimed to explore the clinical significance and prognostic value of multifocality in children and adolescents with PTC. METHODS: This study retrospectively analysed the clinicopathological characteristics and postoperative follow-up data of 338 PTC patients aged ≤ 20 years from May 2012 to July 2022. The clinical and pathological characteristics of 205 patients with unifocal lesions and 133 patients with multifocal lesions were compared. A logistic regression model evaluated the relationship between multifocal lesions and disease recurrence/persistence in children and adolescents with PTC. Based on the median follow-up time of children with multifocal PTC, 114 patients with multifocal PTC older than 20 years were added, and the clinicopathological characteristics were compared between the 133. paediatric/adolescent patients and 114 adult patients with multifocal PTC. RESULTS: Among the paediatric and adolescent patients, over a median follow-up time of 49 months, 133 had multifocal disease and 205 had unifocal disease. Multifocal PTC patients exhibited stronger invasiveness in the form of extrathyroidal extension, tumour diameter, lymph node metastasis, and distant metastasis. Multifocality (OR 2.68; p = 0.017), lateral lymph node metastasis (OR 2.85; p = 0.036), and distant metastasis (OR 4.28; p = 0.010) were identified as independent predictive factors for the recurrence/persistence of disease. Comparing the paediatric/adolescent vs. adult multifocal patients, the former demonstrated greater tumour invasiveness. Lateral lymph node metastasis (OR 6.36; P = 0.012) and distant metastasis (OR 3.70; P = 0.027) were independent predictive factors for recurrence/persistence of disease in multifocal patients, while age was not (OR 0.95; P = 0.455). CONCLUSION: Tumour multifocality independently predicts persistent/recurrent disease in paediatric and adolescent PTC patients.

Polymer-based cascaded waveguide Bragg grating for blood glucose sensing.

Waveguide Bragg grating (WBG) blood glucose sensing, as a biological sensing technology with broad application prospects, plays an important role in the fields of health management and medical treatment. In this work, a polymer-based cascaded WBG is applied to glucose detection. We investigated photonic devices with two different grating structures cascaded-a crossed grating and a bilateral grating-and analyzed the effects of the crossed grating period, bilateral grating period, and number of grating periods on the sensing performance of the glucose sensor. Finally, the spectral reflectance characteristics, response time, and sensing specificity of the cascaded WBG were evaluated. The experimental results showed that the glucose sensor has a sensitivity of 175 nm/RIU in a glucose concentration range of 0-2 mg/ml and has the advantages of high integration, a narrow bandwidth, and low cost.

GC-IMS and multivariate analyses of volatile organic components in different Chinese breeds of chickens.

This study examined the difference in volatile flavor characteristics among four different local breeds of chicken by headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with multivariate analysis. In total, 65 volatile organic compounds (VOCs) were identified (17 aldehydes, 12 alcohols, 7 ketones, 5 esters, 2 acids, and 22 unidentified, i.e., 26.15% aldehydes, 18.46% alcohols, 10.77% ketones, 7.69% esters, 3.08% acids, and 33.84% unidentified), of which 43 were annotated. The chicken meats from the four breeds exhibited good separation in topographic plots, VOC fingerprinting, and multivariate analysis. Meanwhile, 20 different volatile components, with variable importance in projection value > 1, were selected as potential markers to distinguish different breeds of chicken by partial least squares discriminant analysis (PLS-DA). These findings provide insights into the flavor traits of chicken meat. Also, HS-GC-IMS combined with multivariate analysis can be a convenient and powerful method for characterizing different meats.

Sciellin promotes the development and progression of thyroid cancer through the JAK2/STAT3 signaling pathway.

Thyroid cancer (TC) is one of the most common endocrine tumors worldwide. Sciellin (SCEL) is involved in various disease processes, including burn wound healing and neutrophil extracellular traps (NETs); it is highly expressed in TC. However, its biological impact on TC and related mechanisms remain unclear. This study aimed to investigate the effect of SCEL on the function of human TC cell lines B-CPAP and OCUT-2C (cancer cell lines with BRAF V600E mutations). Analyses of data sets and clinical samples revealed enhanced expression of SCEL in TC than in adjacent normal tissue. SCEL knockout suppresses proliferation and cell cycle progression in TC cells, and these results were reversed by the upregulated SCEL expression in TC. SCEL knockout inhibited tumor development in xenograft mouse models. Western blot (WB) demonstrated that the expression of p-JAK2 and p-STAT3 was reduced in SCEL-knockdown TC. These results suggest that SCEL plays a key role in TC progression through the JAK2-STAT3 pathway. Therefore, SCEL can be considered a potential diagnostic biomarker and therapeutic target for TC.

Multi-omics analysis-based macrophage differentiation-associated papillary thyroid cancer patient classifier.

BACKGROUND: The reclassification of Papillary Thyroid Carcinoma (PTC) is an area of research that warrants attention. The connection between thyroid cancer, inflammation, and immune responses necessitates considering the mechanisms of differential prognosis of thyroid tumors from an immunological perspective. Given the high adaptability of macrophages to environmental stimuli, focusing on the differentiation characteristics of macrophages might offer a novel approach to address the issues related to PTC subtyping. METHODS: Single-cell RNA sequencing data of medullary cells infiltrated by papillary thyroid carcinoma obtained from public databases was subjected to dimensionality reduction clustering analysis. The RunUMAP and FindAllMarkers functions were utilized to identify the gene expression matrix of different clusters. Cell differentiation trajectory analysis was conducted using the Monocle R package. A complex regulatory network for the classification of Immune status and Macrophage differentiation-associated Papillary Thyroid Cancer Classification (IMPTCC) was constructed through quantitative multi-omics analysis. Immunohistochemistry (IHC) staining was utilized for pathological histology validation. RESULTS: Through the integration of single-cell RNA and bulk sequencing data combined with multi-omics analysis, we identified crucial transcription factors, immune cells/immune functions, and signaling pathways. Based on this, regulatory networks for three IMPTCC clusters were established. CONCLUSION: Based on the co-expression network analysis results, we identified three subtypes of IMPTCC: Immune-Suppressive Macrophage differentiation-associated Papillary Thyroid Carcinoma Classification (ISMPTCC), Immune-Neutral Macrophage differentiation-associated Papillary Thyroid Carcinoma Classification (INMPTCC), and Immune-Activated Macrophage differentiation-associated Papillary Thyroid Carcinoma Classification (IAMPTCC). Each subtype exhibits distinct metabolic, immune, and regulatory characteristics corresponding to different states of macrophage differentiation.

Ibuprofen inhibits anaplastic thyroid cells in vivo and in vitro by triggering NLRP3-ASC-GSDMD-dependent pyroptosis.

Pyroptosis is a novel type of proinflammatory programmed cell death that is associated with inflammation, immunity, and cancer. Anaplastic thyroid carcinoma (ATC) has a high fatality rate, and there is no effective or standard treatment. The disease progresses rapidly and these tumors can invade the trachea and esophagus, leading to breathing and swallowing difficulties. Hence, new treatment methods are greatly needed. Ibuprofen is a common drug that can exert antitumor effects in some cancers. In this study, we demonstrated in vitro and in vivo that ibuprofen can induce ATC pyroptosis. Hence, we treated C643 and OCUT-2C ATC cells with ibuprofen and found that several dying cells presented the characteristic morphological features of pyroptosis, such as bubble-like swelling and membrane rupture, accompanied by activation of ASC and NLRP3 and cleavage of GSDMD. Along with the increased release of LDH, ibuprofen treatment promoted apoptosis and inhibited viability, invasion, and migration. However, overexpression of GSDMD significantly inhibited ibuprofen-induced pyroptosis. In vivo, research has demonstrated that thyroid tumor growth in nude mice can be suppressed by ibuprofen-induced pyroptosis in a dose-dependent manner. In this research, we explored a new mechanism by which ibuprofen inhibits ATC growth and progression and highlighted its promise as a therapeutic agent for ATC.

Fabrication and characterization of polymer optical waveguide Bragg grating for pulse signal sensing.

Polymer materials have the advantages of a low Young's modulus and low-cost preparation process. In this paper, a polymer-based optical waveguide pressure sensor based on a Bragg structure is proposed. The change in the Bragg wavelength in the output spectrum of the waveguide Bragg grating (WBG) is used to linearly characterize the change in pressure acting on the device. The polymer-based WBG was developed through a polymer film preparation process, and the experimental results show that the output signal of the device has a sensitivity of 1.275 nm/kPa with a measurement range of 0-12 kPa and an accuracy of 1 kPa. The experimental results indicate that the device already perfectly responds to a pulse signal. It has significant potential application value in medical diagnostics and health testing, such as blood pressure monitoring, sleep quality monitoring, and tactile sensing.

Microring structure for flexible polymer waveguide-based optical pressure sensing.

Flexible pressure sensors provide a promising platform for artificial smart skins, and photonic devices provide a new technique to fabricate pressure sensors. Here, we present a flexible waveguide-based optical pressure sensor based on a microring structure. The waveguide-based optical pressure sensor is based on a five-cascade microring array structure with a size of 1500 µm × 500 µm and uses the change in output power to linearly characterize the change in pressure acting on the device. The results show that the device has a sensing range of 0-60 kPa with a sensitivity of 23.14 µW/kPa, as well as the ability to detect pulse signals, swallowing, hand gestures, etc. The waveguide-based pressure sensors offer the advantages of good output linearity, high integration density and easy-to-build arrays.

Full Complex-Amplitude Modulation of Surface Waves Based on Spin-Decoupled Metasurface.

This work proposes a method for surface wave (SW) coupling along with flexible complex amplitude modulation of its wavefront. The linearly polarized incident plane wave is coupled into the surface mode with complex wavefront by exploiting the spin-decouple nature of a reflective chiral meta-atom. As verification, two kinds of metasurface couplers are designed. The first kind contains two examples for SW airy beam generation with and without deflection under linearly polarized illumination, respectively. The second kind is a bi-functional device capable of SW focusing under left-handed circularly polarized illumination, and propagating wave deflection under right-handed circularly polarized illumination, respectively, to verify the fundamental spin-decoupled character. Simulated and experimental results are in good agreement. We believe that this method provides a flexible approach for complex SW applications in integrated optics, optical sensing, and other related fields.

Dysregulation of Ruminococcaceae and Megamonas could be predictive markers for rapid progression of mild cognitive impairment.

BACKGROUND: Intestinal flora dysregulation may affect the development of Alzheimer's disease (AD). However, the correlation between intestinal flora and rapid progression of mild cognitive impairment (MCI) is rarely reported. Our aim was to investigate the features of the intestinal flora in patients with rapidly progressive MCI. METHOD: A total of 1013 participants were screened, in which 87 patients with MCI were followed up for two years. At the baseline time point, fecal samples of the patients were sequenced via the microbial diversity high-throughput 16 s-rDNA. RESULTS: After a two-year follow-up, 30 patients with MCI presented rapidly progressive cognitive impairment, whereas the 57 patients remained unprogressive. Analyses of their fecal samples showed that the abundance of 11 intestinal microflora were significantly different between the two groups at the baseline time point. Further analyses revealed that the decrease of Ruminococcaceae abundance and the increase of Megamonas abundance were significantly correlated with the progression of MCI. Also, the decreased Ruminococcaceae was independently associated with several factors such as P-tau181, and the increased Megamonas was independently associated with diabetes, low-density lipoprotein, median cell count. CONCLUSION: The decrease of Ruminococcaceae and the increase of Megamonas could act as predictive markers for the rapidly progressive MCI.

LncRNA SOCS2-AS1 promotes the progression of papillary thyroid cancer by destabilizing p53 protein.

Long noncoding RNAs (lncRNAs) have been shown to contribute to tumorigenesis and cancer progression. However, neither the dysregulation nor the functions of anti-sense lncRNAs in papillary thyroid carcinoma (PTC) have been exhaustively studied. In this study, we accessed The Cancer Genome Atlas (TCGA) database and discovered that the natural antisense lncRNA SOCS2-AS1 is highly expressed in PTC and that patients with a higher level of SOCS2-AS1 had a poor prognosis. Furthermore, loss- and gain-function assays demonstrated that SOCS2-AS1 promotes PTC cell proliferation and growth both in vitro and in vivo. In addition, we demonstrated that SOCS2-AS1 regulates the rate of fatty acid oxidation (FAO) in PTC cells. Analysis of the mechanism revealed that SOCS2-AS1 binds to p53 and controls its stability in PTC cell lines. Overall, our findings showed that the natural antisense lncRNA SOCS2-AS1 stimulates the degradation of p53 and enhances PTC cell proliferation and the FAO rate.

Multifunctional cellulose-based aerogel for intelligent fire fighting.

Multifunctional biomass-based aerogels with mechanically robust and high fire safety are urgently needed for the development of environmentally-friendly intelligent fire fighting but challenging. Herein, a novel polymethylsilsesquioxane (PMSQ)/cellulose/MXene composite aerogel (PCM) with superior comprehensive performance was fabricated by ice-induced assembly and in-situ mineralization. It exhibited light weight (16.2 mg·cm-3), excellent mechanical resilience, and rapidly recovered after being subjected to the pressure of 9000 times of its own weight. Moreover, PCM demonstrated outstanding thermal insulation, hydrophobicity and sensitive piezoresistive sensing. In addition, benefiting from the synergism of PMSQ and MXene, PCM displayed good flame retardancy and improved thermostability. The limiting oxygen index of PCM was higher than 45.0 %, and it quickly self-extinguished after being removed away from fire. More importantly, the rapid electrical resistance reduction of MXene at high temperature endowed PCM with sensitive fire-warning capability (trigger time was less than 1.8 s), which provided valuable time for people to evacuate and relief. This work provides new insights for the preparation and application of the next-generation high performance biomass-based aerogels.

Low half-wave voltage polymeric electro-optic modulator using CLD-1/PMMA for electrocardiogram (ECG) signal acquisition.

Electro-optic (EO) modulators are typically made of inorganic materials such as lithium niobate; the replacement of these modulators with organic EO materials is a promising alternative due to their lower half-wave voltage (Vπ), ease of handling, and relatively low cost. We propose the design and fabrication of a push-pull polymer electro-optic modulator with voltage-length parameters (VπL) of 1.28 V·cm. The device uses a Mach-Zehnder structure and is made of a second-order nonlinear optical host-guest polymer composed of a CLD-1 chromophore and PMMA polymer. The experimental results show that the loss is 1.7 dB, Vπ drops to 1.6 V, and the modulation depth is 0.637 dB at 1550 nm. The results of a preliminary study show that the device is capable of efficiently detecting electrocardiogram (ECG) signals with performance on par with that of commercial ECG devices.

Complex-amplitude modulation of surface waves based on a metasurface coupler.

Simultaneous and independent modulation of the amplitude and phase of surface waves (SWs) is critical in photonics and plasmonics. Here, we propose a method for flexible complex-amplitude modulation of SWs based on a metasurface coupler. Benefiting from the full range complex-amplitude modulation ability of the meta-atoms over the transmitted field, the coupler can convert the incident wave into a driven surface wave (DSW) with an arbitrary combination of amplitude and initial phase. By placing a dielectric waveguide that supports guided SWs below the coupler, the DSWs can resonantly couple to SWs while preserving complex-amplitude modulation. The proposed scheme provides a practical way for freely tailoring the phase and amplitude profiles of SWs wavefronts. As verification, meta-devices for normal and deflected SW Airy beam generation and SW dual focusing are designed and characterized in the microwave regime. Our findings may stimulate various advanced surface optical meta-devices.

A novel pharmacological mechanism of anti-cancer drugs that induce pyroptosis.

Pyroptosis is an inflammasome-induced lytic form of programmed cell death, and its main effect involves the release of inflammatory mediators when a cell dies, resulting in an inflammatory response in the body. The key to pyroptosis is the cleavage of GSDMD or other gasdermin families. Some drugs can cause cleavage GSDMD or other gasdermin members cause pyroptosis and suppress cancer growth and development. This review explores several drugs that may induce pyroptosis, thereby contributing to tumor treatment. Pyroptosis-inducing drugs, such as arsenic, platinum, and doxorubicin, were used originally in cancer treatment. Other pyroptosis-inducing drugs, such as metformin, dihydroartemisinin, and famotidine, were used to control blood glucose, treat malaria, and regulate blood lipid levels and are effective tumor treatments. By summarizing drug mechanisms, we provide a valuable basis for treating cancers by inducing pyroptosis. In future, the use of these drugs may contribute to new clinical treatments.

Mitochondria-associated endoplasmic reticulum membranes and cardiac hypertrophy: Molecular mechanisms and therapeutic targets.

Cardiac hypertrophy has been shown to compensate for cardiac performance and improve ventricular wall tension as well as oxygen consumption. This compensatory response results in several heart diseases, which include ischemia disease, hypertension, heart failure, and valvular disease. Although the pathogenesis of cardiac hypertrophy remains complicated, previous data show that dysfunction of the mitochondria and endoplasmic reticulum (ER) mediates the progression of cardiac hypertrophy. The interaction between the mitochondria and ER is mediated by mitochondria-associated ER membranes (MAMs), which play an important role in the pathology of cardiac hypertrophy. The function of MAMs has mainly been associated with calcium transfer, lipid synthesis, autophagy, and reactive oxygen species (ROS). In this review, we discuss key MAMs-associated proteins and their functions in cardiovascular system and define their roles in the progression of cardiac hypertrophy. In addition, we demonstrate that MAMs is a potential therapeutic target in the treatment of cardiac hypertrophy.

Silicon-photonics-based waveguide Bragg grating sensor for blood glucose monitoring.

We demonstrated the design of two different structures, a two-sided structure and a top-surface structure, of glucose waveguide Bragg grating (WBG) sensors in a single-mode silicon-on-insulator (SOI) chip. A two-sided WBG structure was fabricated, and chip preparation was realized by lithography and other processes. A photonic platform for testing the two-sided WBG using glucose was built and completed. When the blood glucose concentration changed by 1 mg/mL, the two-sided WBG had a wavelength offset of 78 pm. The experimental results show that the two structures can achieve the sensing of different blood glucose concentrations. The two-sided WBG had better sensing performance and thus has a wide range of application prospects.

Defect chemistry of electrocatalysts for CO2 reduction.

Electrocatalytic CO2 reduction is a promising strategy for converting the greenhouse gas CO2 into high value-added products and achieving carbon neutrality. The rational design of electrocatalysts for CO2 reduction is of great significance. Defect chemistry is an important category for enhancing the intrinsic catalytic performance of electrocatalysts. Defect engineering breaks the catalytic inertia inherent in perfect structures by imparting unique electronic structures and physicochemical properties to electrocatalysts, thereby improving catalytic activity. Recently, various defective nanomaterials have been studied and show great potential in electrocatalytic CO2 reduction. There is an urgent need to gain insight into the effect of defects on catalytic performance. Here, we summarized the recent research advances on the design of various types of defects, including carbon-based materials (intrinsic defects, heteroatom doping and single-metal-atom sites) and metal compounds (vacancies, grain boundaries, and lattice defects). The major challenges and prospects of defect chemistry in electrocatalytic CO2 reduction are also proposed. This review is expected to be instructive in the development of defect engineering for CO2 reduction catalysts.

Generation of 2D Airy beams with switchable metasurfaces.

Airy beams exhibit intriguing characteristics, such as diffraction-free propagation, self-acceleration, and self-healing, which have aroused great research interest. However, the spatial light modulator that generates Airy beams has problems such as narrow operational bandwidth, high cost, poor phase discretization, and single realization function. In the visible region (λ∼532 nm), we proposed a switchable all-dielectric metasurface for generating transmissive and reflective two-dimensional (2D) Airy beams. The metasurface was mainly composed of titanium dioxide nanopillars and vanadium dioxide substrate. Based on the Pancharatnam-Berry phase principle, a high-efficient Airy beam can be generated by controlling the phase transition of vanadium dioxide and changing the polarization state of the incident light. The optimized optical intensity conversion efficiencies of the transmissive and reflective metasurfaces were as high as 97% and 70%, respectively. In the field of biomedical and applied physics, our designed switchable metasurface is expected to offer the possibility of creating compact optical and photonic platforms for efficient generation and dynamic modulation of optical beams and open up a novel path for the application of high-resolution optical imaging systems.

Functional Epoxy Elastomer Integrating Self-Healing Capability and Degradability for a Flexible Stretchable Strain Sensor.

With the rapid development of flexible electronics and the increasing deterioration of the natural environment, functional and environmentally friendly flexible strain sensors have become one of the frontier research hotspots. Here, we propose a novel strategy to synthesize a functional epoxy elastomer integrating self-healing capability and degradability for flexible stretchable strain sensors. A carboxyl-terminated epoxy prepolymer was first synthesized using carboxyl-terminated PEG (PEG-COOH), 2,2'-dithiodibenzoic acid (DTSA), and 1,4-butanediol diglycidyl ether (BDDE), and then crosslinked by epoxidized soybean oil (ESO) to yield an epoxy elastomer. The obtained elastomer exhibited not only high tensile stress (5.07 MPa), large stretchability (477%), and high healing efficiency (92.5%) but also superior degradability in alkaline aqueous solution. The elastomer-based stretchable strain sensor with microstructure showed high sensitivity (GF = 176.71) and was successfully applied for detecting human motions and recognizing objects with various shapes. Moreover, the healed sensor could restore stable sensing ability. The prepared functional epoxy elastomer is of great significance for the preparation of environmentally friendly and high-performance sensors and is promising for applications in the fields of healthcare monitoring, intelligent robots, and wearable electronics.