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1.
World J Clin Cases ; 9(23): 6624-6638, 2021 Aug 16.
Artículo en Inglés | MEDLINE | ID: mdl-34447810

RESUMEN

Nonalcoholic fatty pancreas disease (NAFPD) is an emerging disease that has gained an increasing amount of attention in recent years. It describes fat accumulation in the pancreas with insignificant alcohol consumption, but the pathogenesis is largely unknown. A wide range of terms have been used to describe the phenomenon of pancreatic fat accumulation, but NAFPD remains an under-recognized and non-independent disorder. Obesity, age, sex, race, and unhealthy lifestyle are established independent risk factors for NAFPD, which is strongly associated with metabolic syndrome, type 2 diabetes, pancreatitis, pancreatic fistula, pancreatic cancer, and nonalcoholic fatty liver disease. At present, imaging techniques are common diagnostic aids, but uniform criteria and consensus are lacking. Therapeutically, healthy diet, weight loss, and exercise are the mainstays to reduce pancreatic fat accumulation. It can be seen that there is a limited understanding of NAFPD at this stage and further exploration is needed. Previous studies have revealed that NAFPD may directly affect diagnosis and clinical decision-making. Therefore, exploring the pathophysiological mechanism and clinical associations of NAFPD is a major challenge for researchers and clinicians.

2.
IEEE Trans Cybern ; 51(1): 416-426, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31831461

RESUMEN

This article focuses on the fault-tolerant constrained consensus problem for multiagent systems with communication delays. The communication graphs are first assumed to be directed and fixed. Then, a novel delay-dependent fault-tolerant controller is designed such that, in the presence of communication delays and randomly occurring actuator failures, the influence of the projections and the initial states on the closed-loop system can be attenuated with a prespecified level. Based on the provided performance requirement, the initial state of each agent does not need to be identical. The proposed control algorithms ensure that sufficient conditions are met for the fault-tolerant constrained consensus to be achieved according to the prespecified performance index. After this, the controller gains are computed by employing an iterative linear matrix inequality scheme. Finally, a numerical example is provided to show the effectiveness of the proposed method.

3.
Gut Pathog ; 11: 58, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31788031

RESUMEN

BACKGROUND: Chronic hepatitis B virus (HBV) infection-reduced liver functions are associated with intestinal microbial community dissimilarity. This study aimed to investigate the microbial community dissimilarity in patients with different grades of HBV-related liver cirrhosis. RESULTS: Serum endotoxin was increased with Child-Pugh (CP) class (A, B, and C). Veillonellaceae and Lachnospiraceae families were reduced in patients compared with controls. Megamonas and Veillonella genus was reduced and increased in patients compared with controls, respectively, especially in CPB and CPC groups. Correlation analysis showed that endotoxin content was significantly correlated with alcohol consumption (95% CI 0.100, 0.493), CP class (95% CI 0.289, 0.687) and Lachnospiraceae family level (95% CI - 0.539, - 0.122). Firmicutes/Bacteroidetes ratio was correlated with the level of Lachnospiraceae family (95% CI 0.013, 0.481), Veillonellaceae family (95% CI 0.284, 0.696), Megamonas genus (95% CI 0.101, 0.518) and Veillonella genus (95% CI 0.134, 0.545). All aforementioned bacteria were independent risk or protective factors for hepatitis. Alcohol consumption changed microbial community. CONCLUSIONS: Our study demonstrated that elevated Firmicutes/Bacteroidetes ratio, reduced Megamonas genus level and increased Veillonella genus level were indicators for HBV-related liver cirrhosis. Alcohol-related pathogenesis was associated with the changed microbial community.

5.
Chin Med J (Engl) ; 126(8): 1491-7, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23595383

RESUMEN

BACKGROUND: Many studies on periostin have focused on its role in tumors and vascular reconstruction. However, the effect of periostin on stem cell function remains unclear. The aim of this study was to enhance vitality in adipose-derived stem cells (ADSCs), the effect of periostin on the function of ADSCs was observed. METHODS: Human ADSCs (hADSCs) were isolated from human adipose tissue by collagenase I digestion and collected in multi-periods for in vitro culture. CD29, CD34, CD44, CD45 and CD105 were detected by flow cytometry. In addition, directed differentiation of hADSCs was induced using adipogenic, osteogenic and chondrogenic induction mediums. The induced morphological changes were observed using oil red O, Alizarin red and alcian blue staining. Periostin was administered to hADSCs in an acidic environment. The treatments of cells were divided into three groups: a periostin group (P); an acidic control group (A); a normal group (N). Then the resulting cell proliferation and migration were detected using a Cell Counting Kit-8 (CCK-8) and a transwell chamber assay, respectively. RESULTS: The detection rates of CD29, CD44, CD105, CD34 and CD45 were 98.89%, 93.73%, 86.99%, 0.19% and 0.16%. The specific staining of cells was positive after induction culture. The mean absorbance of the cells in group P and A at 12 hours were 16.67% and 22.22% greater than group N, respectively (P < 0.01). The mean absorbance of cells from group P was 20.00% greater than that of group A at 48 hours (P < 0.05). The mean number of migratory cells per visual field in group A was 50.38% lower than that in group N (P < 0.05). The migratory cell number in group P was 119.98% greater than that in group A (P < 0.05). CONCLUSIONS: The acidic environment impacted hADSC proliferation and inhibited cell migration. However, periostin was able to promote the proliferation and migration of hADSCs despite the acidic environment.


Asunto(s)
Tejido Adiposo/citología , Moléculas de Adhesión Celular/farmacología , Células Madre/efectos de los fármacos , Adulto , Antígenos de Superficie/análisis , Diferenciación Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Femenino , Humanos , Células Madre/fisiología
6.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 24(6): 334-7, 2012 Jun.
Artículo en Chino | MEDLINE | ID: mdl-22681660

RESUMEN

OBJECTIVE: To observe the expression of osteoblast-specific factor 2 (periostin, PN), angiopoietin-1 (Ang-1), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 [VEGFR-2/fetal liver kinase-1 (FLK-1)] in wound surface and its peripheral skin, and their effects on wound healing in rats. METHODS: Forty-eight Sprague-Dawley (SD) rats were randomly divided into six groups, with 8 rats in each group. An area of 2 cm×2 cm full-thickness skin was excised on both sides of the back of rats. Specimens from wounds were obtained on 1, 4, 7, 10, 14, 21 days after operation, and histological evaluation and immunohistochemical staining of PN, Ang-1, VEGF and FLK-1 were made to determine their expression levels. Normal skin specimens were obtained as normal controls. RESULTS: The expressions of PN, Ang-1, VEGF and FLK-1 were significantly increased in wound surface after operation. Compared with the skin of normal controls, the expression of PN in the tissues of wound increased by 234.4% on the 1st day, and then increased continuously up to 597.9% on the 7th day (reaching the peak) after operation, followed by a decrease, the increase rate was 280.9% on the 21st day, and still remained at a high level (all P < 0.05). The expression of Ang-1 in the tissue of wound increased by 128.1% on the 1st day and 327.5% on the 4th day (reaching the peak), and then, it was gradually decreased. The increase rate was only 80.5% on the 14th day and it rose slightly later (all P < 0.05). The expression of VEGF in the tissues of wound reached the peak (165.8%) on the 7th day. Then it decreased with a slight fluctuation (all P < 0.05). The expression of FLK-1 in the tissues of wound was increased by 56.1% on the 1st day, and the level remained. It reached the peak by an increase of 70.1% on the 7th day (both P < 0.05). Then, it was lowered after the 10th day (all P > 0.05). CONCLUSIONS: The expressions of PN, Ang-1, VEGF, FLK-1 were obviously increased during healing of skin wound, with different peaking time and expressing rates. The increase in expression of PN showed the longest duration and highest peak value. The PN, Ang-1, VEGF, FLK-1 all play a role in the wound healing process, and PN might play an important role during the healing process of a full-thickness cutaneous wound.


Asunto(s)
Angiopoyetina 1/metabolismo , Moléculas de Adhesión Celular/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo , Receptor 2 de Factores de Crecimiento Endotelial Vascular/metabolismo , Cicatrización de Heridas , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Piel/lesiones
7.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 27(4): 272-6, 2011 Jul.
Artículo en Chino | MEDLINE | ID: mdl-22097314

RESUMEN

OBJECTIVE: To explore the different expression of TGF-beta1 and collagen during the healing process of wound infected by Pseudomonas aeruginosa (PAO1). METHODS: 24 female Wistar rats were randomly divided into pure wound group (group A) and wound + PAO1 group (group B). The re-epithelial rate, shrinkage rate and neutrophils number on the wounds were observed on the 1st, 3rd, 7th and 10th day after operation. The expression of TGF-beta1 and collage I, Ill was also detected. RESULTS: On the 7th day, the re-epithelial rate in group A was higher than that in group B, while the shrinkage rate in group A was lower than that in group B. The neutrophils number increased to peak on the 1st day in group B, but on the 3rd day in group A. The TGF-beta1 expression increased after operation in both groups, but it decreased in group B on the 3rd day and re-increased after that. The TGF-beta1 expression was significantly different between the two groups on the 7th day (P < 0.05). The expression of collagen I and III decreased during healing. The expression of collagen III in group A was higher on the 3rd day and was lower on the 7th and 10th day than that in group B, showing a significant difference (P < 0.05). CONCLUSIONS: PAO1 infection could delay the expression of TGF-beta1 and collagen I, III on wound, which may interfere the healing process of wound.


Asunto(s)
Colágeno Tipo III/metabolismo , Colágeno Tipo I/metabolismo , Infecciones por Pseudomonas/patología , Infección de la Herida Quirúrgica/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Cicatrización de Heridas , Animales , Femenino , Pseudomonas aeruginosa , Ratas , Ratas Wistar , Trasplante de Piel , Infección de la Herida Quirúrgica/microbiología
8.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 25(4): 447-51, 2008 Aug.
Artículo en Chino | MEDLINE | ID: mdl-18683148

RESUMEN

OBJECTIVE: To study the association of the Pro12Ala and C1431T polymorphism of the PPAR gamma2 gene and their haplotypes with obesity and type 2 diabetes in Chinese population. METHODS: PCR-restriction fragment length polymorphism was used to determine the Pro12Ala and C1431T polymorphisms in 207 patients with type 2 diabetes and 101 non-diabetic control subjects. RESULTS: (1) In non-diabetic control population, the Ala allele frequency was 0.064, the T1431 allele frequency was 0.252. Haplotype analysis showed that the Pro12Ala and C1431T polymorphisms were in linkage disequilibrium (Do=0.63, r(2)=0.074), which constituted three major haplotypes Pro-C, Pro-T and Ala-T. (2) There were no significant differences of the distribution frequencies of the Pro12Ala and C1431T polymorphism and their haplotypes between the type 2 diabetes mellitus group and non-diabetic control group (P > 0.05). (3) The Pro12Ala polymorphism was associated with blood pressure and lipidemia in diabetic patients. The Ala allele significantly decreased the diastolic blood pressure of non-obese diabetic patients (P < 0.05), but it did not benefit to the obese diabetic patients for the lipidemia (P < 0.05). The C1431T polymorphism was associated with overweight and obesity in diabetic patients. The T1431 allele frequency in the body mass index > or = 25 layer was significantly higher than that in the body mass index < 25 layer (P < 0.05). CONCLUSION: The Pro12Ala and C1431T polymorphisms of the PPAR gamma2 gene might not be a major etiological factor for type 2 diabetes; the C1431T polymorphism was associated with overweight or obesity in diabetic patients.


Asunto(s)
Diabetes Mellitus Tipo 2/genética , Frecuencia de los Genes , Haplotipos , Obesidad/genética , PPAR gamma/genética , Alanina/genética , Sustitución de Aminoácidos , Pueblo Asiatico/genética , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/fisiopatología , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Obesidad/fisiopatología , Polimorfismo Genético , Prolina/genética , Triglicéridos/sangre
9.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 24(2): 129-30, 2008 Mar.
Artículo en Chino | MEDLINE | ID: mdl-18590216

RESUMEN

OBJECTIVE: To practice a more atraumatic, physiological and aesthetically valued approach of construction for neovagina. METHODS: Laparoscopically using peritoneum as neovagina lining. RESULTS: From March 2005 to September 2006, this technique was adopted to treat 10 patients whose diagnosis was congenital absence of vagina. The ages of the patients were from 19 to 32. The operation lasted average 2.34 hours. And hospitalization was about 20.5 days. Follow-up ranged from 3 - 12 months. No complication occurred. All of the patients was satisfied with their sexual life. CONCLUSIONS: Laparoscopically assisted neovaginaplasty, in which peritoneum was substituted for vaginal mucous membrane, was a kind of ideal approach of vaginal creation.


Asunto(s)
Laparoscopía , Peritoneo/trasplante , Procedimientos de Cirugía Plástica/métodos , Vagina/cirugía , Adulto , Femenino , Estudios de Seguimiento , Humanos , Vagina/anomalías , Adulto Joven
10.
Di Yi Jun Yi Da Xue Xue Bao ; 25(7): 827-32, 2005 Jul.
Artículo en Chino | MEDLINE | ID: mdl-16027079

RESUMEN

OBJECTIVE: To investigate the effect of high-salt diet on blood pressure, renal morphology and osteopontin (OPN) expression in Sprague-Dawley rat with Ang II-induced renal injury. METHODS: Thirty-six male SD rats aged 12 weeks receiving normal salt diet were rolled in the study, and received Ang II (100 ng.kg-1.min-1) for two weeks using subcutaneous minipump. Rats were then fed high sodium (n=18, 4% NaCl) or normal sodium (n=18, 0.6% NaCl) diet from third week respectively and lasted for 12 weeks. Eighteen rats in the control were sham-operated and fed normal sodium diet. Tail systolic blood pressure (SBP) was determined every two weeks. Expressions of TGF-beta1 OPN mRNA and its protein in SD rat kidney were measured with quantitative RT-PCR and immunohistochemistry respectively at the end of Ang II infusion and at the end of the experiment. Renal ultrastructure was observed by electronmicroscopy. RESULTS: SBP of the rats received Ang II increased significantly compared with controls (P?0.05). SBP returned to normal after Ang II infusion stopped. No significant difference existed in blood pressure between rats fed high salt diet and normal salt diet in the period of 12 weeks (P<0.05). Expressions of TGF-beta1, OPN mRNA and its protein in the kidney of the rats fed with high salt diet were up-regulated at the end of Ang II infusion (P>0.05) and at the end of the 12th week compared with the rats fed normal salt diet and controls (P<0.01). The kidney was damaged by Ang II, which was further aggravated by the infusion of high salt diet. CONCLUSIONS: High salt diet could aggravate the renal injury of the rats with Ang II-induced renal injury that may be independent of blood pressure change. Expressions of the TGF-beta1, OPN mRNA and its protein were up-regulated after renal injury.


Asunto(s)
Presión Sanguínea/efectos de los fármacos , Enfermedades Renales/metabolismo , Osteopontina/biosíntesis , Cloruro de Sodio Dietético/farmacología , Angiotensina II , Animales , Riñón/patología , Enfermedades Renales/inducido químicamente , Enfermedades Renales/patología , Masculino , Osteopontina/genética , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Ratas , Ratas Sprague-Dawley , Factor de Crecimiento Transformador beta1/biosíntesis
11.
Di Yi Jun Yi Da Xue Xue Bao ; 25(5): 595-7, 2005 May.
Artículo en Chino | MEDLINE | ID: mdl-15897150

RESUMEN

OBJECTIVE: To investigate the effect of high-salt diet on the expression of osteopontin (OPN) mRNA and its protein in Sprague-Dawley rat kidney. METHODS: Forty-eight male SD rats aged 10 weeks receiving normal salt diet were enrolled in study, and were divided into two groups and fed with high salt diet (4%NaCl) or normal salt diet (0.6%NaCl) for 12 weeks respectively, with 24 rats in each group. Tail systolic blood pressure and bodyweight were measured in all rats every week. At the end of 4th, 8th and 12th week, 6 rats in each group were sacrificed for detection of the expression of OPN mRNA and its protein in the kidney with quantitatine real-time (QRT)-PCR and immunohistochemistry, respectively. RESULTS: No significant difference was found in blood pressure, bodyweight and kidney weight/bodyweight between high-salt diet group and normal salt diet group (P>0.05). The expression of OPN mRNA (0.27+/-0.16 vs 0.15+/-0.13, P<0.05) and its protein (0.78+/-0.15 vs 0.61+/-0.11, P<0.01) in SD rat kidney with high-salt diet were up-regulated at the end of 12th week compared with the rats with normal salt diet. CONCLUSIONS: High-salt diet increase the expression of OPN mRNA and its protein in SD rat kidney. Renal injury induced by high salt intake might be independent of blood pressure change.


Asunto(s)
Riñón/metabolismo , Osteopontina/biosíntesis , Cloruro de Sodio Dietético/administración & dosificación , Animales , Presión Sanguínea , Masculino , Osteopontina/genética , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Ratas , Ratas Sprague-Dawley
12.
Zhonghua Wai Ke Za Zhi ; 43(23): 1537-40, 2005 Dec 01.
Artículo en Chino | MEDLINE | ID: mdl-16412293

RESUMEN

OBJECTIVE: To examine the expression of nestin and neurogenin 3 (Ngn3), the markers of pancreatic stem cells, in the human fetal pancreas. METHODS: The human fetal pancreas tissue of 12 and 14 weeks were examined for the expression of nestin and Ngn3 using the techniques of immunofluorescence dye and RT-PCR. RESULTS: Both nestin and Ngn3 expressed widely in 12 and 14 weeks before in human fetal pancreatic tissue. In these positive cells there was no co-expressing insulin or glucagon. There were nestin and Ngn3 co-expressing cells in ducts but not in the islets. The results of RT-PCR also indicated the expression of nestin and Ngn3. CONCLUSIONS: There was no expression of the markers of mature endocrine cells in the nestin and Ngn3 positive cells, and they were the marks of no-differentiation cells in the human fetal pancreatic tissue.


Asunto(s)
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/biosíntesis , Proteínas de Filamentos Intermediarios/biosíntesis , Proteínas del Tejido Nervioso/biosíntesis , Páncreas/citología , Páncreas/metabolismo , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Fluoroinmunoensayo , Humanos , Técnicas In Vitro , Proteínas de Filamentos Intermediarios/genética , Microscopía Fluorescente , Proteínas del Tejido Nervioso/genética , Nestina , Páncreas/embriología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
13.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 20(2): 94-7, 2004 Mar.
Artículo en Chino | MEDLINE | ID: mdl-15334926

RESUMEN

OBJECTIVE: To illustrate the effects of the site and method of liposuction on differentiation of human preadipocytes. METHODS: Forty-two fatty samples were obtained with liposuction, which were then divided into four groups according to operation sites (abdomen, hip or extremity) and the methods (conventional negative-pressure or syringe method). Each sample was treated with collagenase I to release preadipocytes for in vitro culture. Affected by the differentiation-induced agents for 9 days or 28 days, the cultured adipocytes were stained with Nile red and observed under a fluorescent microscope. The differentiation rates were examined with flow cytometric analysis and the quantity of intracytoplasmic lipids was determined with oil red O staining. The results were analyzed with independent samples t-test (Mann-Whitney) using SPSS 10.0. RESULTS: There was no statistical difference in differentiation at the 9th day or 28th day among the preadipocytes obtained from the abdomen, hip or extremity with the negative-pressure method. The preadipocytes from the abdomen differentiated more at the 28th day than the 9th day (P < 0.05), which was not observed in the hip or the extremity. The preadipocytes obtained from the abdomen with the negative-pressure method differentiated more than those with the syringe method (P < 0.05). CONCLUSION: No essential difference was found in preadipocyte differentiation among the liposuction sites, while the abdomen might have some superiority. The negative-pressure method of liposuction is the first option in future research of tissue engineering. The flow cytometric analysis is a convenient way to study preadipocyte differentiation.


Asunto(s)
Adipocitos/citología , Diferenciación Celular , Lipectomía/métodos , Abdomen/cirugía , Adulto , Células Cultivadas , Femenino , Citometría de Flujo , Cadera/cirugía , Humanos , Masculino , Microscopía Fluorescente , Persona de Mediana Edad , Oxazinas
14.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 19(2): 85-7, 2003 Mar.
Artículo en Chino | MEDLINE | ID: mdl-12889180

RESUMEN

OBJECTIVE: To evaluate a method to partially remove gastrocnemius for improvement of the contour of the leg. METHODS: Thirteen patients with bulked calf were undergoing the treatment. With a self-designed apparatus, the gastrocnemius was partially removed by placing the device in the muscle through an incision in the popliteal fossa. The follow-ups were carried out for 1-6 months. RESULTS: Thirteen patients were successfully treated by above mentioned technique. The removed amount of muscle was weighted between 11 g and 201 g, averaged 77 g. All of the patients could be able to walk three days after the operation. But, the normal walk had to take 1 month after of the surgery for recovering. The function of the ankle joint was not obviously influenced. CONCLUSION: The above mentioned technique is a safe and effective method for re-contouring the leg.


Asunto(s)
Técnicas Cosméticas , Pierna/cirugía , Músculo Esquelético/cirugía , Ambulación Precoz , Humanos , Pierna/anatomía & histología , Factores de Tiempo
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