Enzyme stability in polymer hydrogel-enzyme hybrid nanocarrier containing phosphorylcholine group.

Enzymes are biological catalysts with good biocompatibility and high efficiency and have been widely used in many fields, such as wastewater treatment, biosensors, and the medical industry. However, their inherently low stability under conditions of practical use limits further applications. Zwitterionic polymers possessing a pair of oppositely charged groups in their repeating units can increase protein stability because of their good biocompatibility and high water content. In this study, zwitterionic copolymer nanogels comprising poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-methacrylic acid-N-hydroxy succinimide ester (MNHS)) (PMS) were synthesized via reversible addition-fragmentation chain-transfer polymerization (RAFT). ß-Galactosidase (ß-gal) was post-modified within zwitterionic polymer nanogels with a covalently-bound spacer and the activity was compared with that of directly immobilized ß-gal and free ß-gal. Compared with direct immobilization, covalent immobilization with a spacer could reduce the structural change of ß-gal, as confirmed by the circular dichroism spectra. Although the activity of ß-gal decreased after immobilization, the hybrids of the ß-gal immobilized nanogels, termed hybrid nanogel-enzymes, demonstrated superior stability compared to the free enzymes. The hybrid nanogel-enzymes maintained their function against inactivation by organic solvents and proteinases owing to their high water content, anti-biofouling properties, and limited mass transfer. They can also withstand protein aggregation at high temperatures and maintain their activity. Compared to direct immobilization, immobilization with a spacer resulted in a dramatic increase in the enzyme activity and a slight decrease in the stability. These results indicate that polymer nanogels containing phosphorylcholine units are promising materials for enzyme immobilization, expanding the scope of enzyme applications.

Lactiplantibacillus sp. G6 isolated from goose intestine as starter culture for degrading nitrite and improving quality in Chinese pickle fermentation.

Animal intestines is considered as a source of lactic acid bacteria (LAB) that have potential to decrease the nitrite level during fermentation of food such as pickles. It was hypothesized that optimized level of LAB has a high capacity to degrade nitrite during Chinese pickle fermentation and benefit a higher acceptability of the Chinese pickle product. This study aims to investigate the performance of a goose intestine-isolated LAB strain G6 under the species Lactiplantibacillus plantarum as a starter culture of Chinese pickles. The results showed that Lactiplantibacillus sp. G6 had a nitrite degradation rate close to 100% under the MRS broth condition of 25 °C, 2% inoculum volume and pH at 5. As a starter culture for Chinese pickle, this strain was able to achieve a higher LABs amount, lower nitrite residue after fermentation, compared with the group without the starter, which implicates its feasibility of applying on fermented food for reducing nitrite level. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-023-01433-8.

Bacterial community succession and the enrichment of antibiotic resistance genes on microplastics in an oyster farm.

Microplastics can be colonized by microorganisms and form plastisphere. However, knowledge of bacterial community succession and the enrichment of antibiotic resistance genes (ARGs) and pathogens on microplastics in aquaculture environments is limited. Here, we conducted a 30-day continuous exposure experiment at an oyster farm. Results showed that the alpha-diversity of communities on most microplastics continuously increased and was higher than in planktonic communities after 14 days. Microplastics could selectively enrich certain bacteria from water which can live a sessile lifestyle and promote colonization by other bacteria. The composition and function of plastisphere communities were distinct from those in the surrounding water and influenced by polymer type and exposure time. Microplastics can enrich ARGs (sul1, qnrS and blaTEM) and harbor potential pathogens (e.g., Pseudomonas aeruginosa). Therefore, microplastic pollution may pose a critical threat to aquaculture ecosystems and human health. Our study provides further insight into the ecological risks of microplastics.

Effects of Spraying KH2PO4 on Flag Leaf Physiological Characteristics and Grain Yield and Quality under Heat Stress during the Filling Period in Winter Wheat.

As one of the most important wheat-producing areas in China, wheat is prone to heat stress during the grain filling period in the Huang-Huai-Hai Plain (3HP), which lowers yields and degrades the grain quality of wheat. To assess the effects of spraying potassium dihydrogen phosphate (KH2PO4) on the physiological traits in flag leaves and grain yield (GY) and quality under heat stress during the filling period, we conducted a two-year field experiment in the winter wheat growing seasons of 2020-2022. In this study, spraying water combined with heat stress (HT), 0.3% KH2PO4 (KDP), and 0.3% KH2PO4 combined with heat stress (PHT) were designed, and spraying water alone was used as a control (CK). The dates for the spraying were the third and eleventh day after anthesis, and a plastic film shed was used to impose heat stress on the wheat plants during the grain filling period. The results showed that spraying KH2PO4 significantly improved the chlorophyll content and net photosynthesis rate (Pn) in flag leaves compared with the non-sprayed treatments. Compared with CK, the Pn in HT decreased by 8.97% after heat stress, while Pn in PHT decreased by 7.44% compared to that of KDP. The activities of superoxide dismutase, catalase, and peroxidase in flag leaves were significantly reduced when the wheat was subjected to heat stress, while malonaldehyde content increased, and the enzyme activities were significantly enhanced when KH2PO4 was sprayed. Heat stress significantly decreased the contribution rate of dry matter accumulation (DM) after anthesis of wheat to grain (CRAA), whereas spraying KH2PO4 significantly increased the CRAA and harvest index. At maturity, the DM in CK was significantly higher than that in HT, KDP was significantly higher than PHT, and KDP had the highest DM. Compared with CK, the GY in KDP significantly increased by 9.85% over the two years, while the GY in HT decreased by 11.44% compared with that of CK, and the GY in PHT decreased by 6.31% compared to that of KDP. Spraying KH2PO4 after anthesis primarily helped GY by maintaining a high thousand grain weight to lessen the negative effects of heat stress on wheat. Moreover, heat stress significantly reduced protein concentration, wet gluten content, dough development time, and hardness index in grains of mature, while spraying KH2PO4 maintained a sufficient grain quality under the conditions of achieving higher yields. Overall, spraying KH2PO4 after anthesis could enhance the heat stress resistance of wheat and maintain the photosynthetic capacity of flag leaves, ensuring the dry matter production and reducing the negative effects on grain yield and quality in the 3HP.

Stilbene-based derivatives as potential inhibitors of trimethylamine (TMA)-lyase affect gut microbiota in coronary heart disease.

Coronary heart disease (CHD) is defined by atherosclerosis, which may result in stenosis or blockage of the arterial cavity, leading to ischemic cardiac diseases such as angina and myocardial infarction (MI). Accumulating evidence indicates that the gut microbiota play a critical role in the initiation and progression of CHD. The gut microbial metabolite trimethylamine N-oxide (TMAO) is intimately linked to the pathophysiology of CHD. The hepatic flavin-containing monooxygenases (FMOs) convert trimethylamine (TMA) to TMAO. As a result, it is critical to prevent TMA generation. Stilbenes could reduce cardiovascular disease mortality. Twelve stilbenes with inhibitory activity against TMA-lyase were compiled and evaluated in this study. Docking results showed Resveratroloside had the highest Vina score, indicating that it was the most active and might be employed as a lead molecule for further structural modification.

[Determination of Dichloromethane in Blood with Headspace Gas Chromatography and Gas Chromatography-Mass Spectrometry].

Objective: To establish a method for qualitative determination of dichloromethane (DCM) in blood by gas chromatography-mass spectrometry (GC-MS) and quantitative determination of DCM in blood by headspace gas chromatography (HS-GC), and to provide reliable support for forensic examination and analysis of poisoning or deaths caused by DCM. Methods: 0.5 mL blood sample was collected, added into headspace vial with chloroform as the internal standard, and processed by heating at 65 °C and evacuation treatment. The intermediate gas in the headspace vial was analyzed by GC-MS for qualitative validation of the method and by HS-GC for quantitative validation of the method. The method was then applied in forensic case analysis. Results: Qualitative validation of the examination method by GC-MS found that the chromatographic peak and mass spectral characteristic ions were specific in samples added with DCM, and that no interference was observed in the blank negative samples. The limit of detection (LOD) was 5 µg/mL. Quantitative method validation by HS-GC found that the chromatographic peak of DCM was well separated from those of eight other volatile compounds, with the resolution>1.5 in all cases; the lower limit of quantification (LOQ) was 20 µg/mL and good linearity was shown within the range of 20 and 1000 µg/mL, R>0.999; the intra-day test precision and inter-day test precision were good (relative standard deviation, or RSD<15% for both) and test accuracy was high (relative error, or δ<15%). With the method established in the study, DCM was detected successfully in the blood of two fatal cases caused by DCM poisoning, with the blood concentration being 470 µg/mL and 915 µg/mL, respectively. Conclusion: This method is shown to be a rapid, stable and accurate approach to the qualitative and quantitative forensic and toxicological analysis of DCM in blood in DCM poisoning cases or deaths caused by DCM.

Overcoming Reproductive Compromise Under Heat Stress in Wheat: Physiological and Genetic Regulation, and Breeding Strategy.

The reproductive compromise under heat stress is a major obstacle to achieve high grain yield and quality in wheat worldwide. Securing reproductive success is the key solution to sustain wheat productivity by understanding the physiological mechanism and molecular basis in conferring heat tolerance and utilizing the candidate gene resources for breeding. In this study, we examined the performance on both carbon supply source (as leaf photosynthetic rate) and carbon sink intake (as grain yields and quality) in wheat under heat stress varying with timing, duration, and intensity, and we further surveyed physiological processes from source to sink and the associated genetic basis in regulating reproductive thermotolerance; in addition, we summarized the quantitative trait loci (QTLs) and genes identified for heat stress tolerance associated with reproductive stages. Discovery of novel genes for thermotolerance is made more efficient via the combination of transcriptomics, proteomics, metabolomics, and phenomics. Gene editing of specific genes for novel varieties governing heat tolerance is also discussed.

Dry pick-and-flip assembly of van der Waals heterostructures for microfocus angle-resolved photoemission spectroscopy.

We present a dry pick-and-flip assembly technique for angle-resolved photoemission spectroscopy (ARPES) of van der Waals heterostructures. By combining Elvacite2552C acrylic resin and 1-ethyl-3-methylimidazolium ionic liquid, we prepared polymers with glass transition temperatures (Tg) ranging from 37 to 100 â„ƒ. The adhesion of the polymer to the 2D crystals was enhanced at [Formula: see text]. By utilizing the difference in [Formula: see text], a 2D heterostructure can be transferred from a high-[Formula: see text] polymer to a lower-[Formula: see text] polymer, which enables flipping its surface upside down. This process is suitable for assembling heterostructures for ARPES, where the top capping layer should be monolayer graphene. The laser-based micro-focused ARPES measurements of 5-layer WTe2, 3-layer MoTe2, 2-layer WTe2/few-layer Cr2Ge2Te6, and twisted double bilayer WTe2 demonstrate that this process can be utilized as a versatile sample fabrication method for investigating the energy spectra of 2D heterostructures.

Comparative Physiology and Transcriptome Analysis of Young Spikes in Response to Late Spring Coldness in Wheat (Triticum aestivum L.).

Late spring coldness (LSC) is critical for wheat growth and development in the Huang-Huai valleys of China. However, little is known about the molecular mechanisms for young spikes responding to low temperature (LT) stress during anther connective tissue formation phase (ACFP). To elucidate the molecular mechanisms associated with low temperature, we performed a comparative transcriptome analysis of wheat cultivars Xinmai26 (XM26: cold-sensitive) and Yannong19 (YN19: cold-tolerant) using RNA-seq data. Over 4000 differently expressed genes (DEGs) were identified under low temperature conditions (T1: 4°C) and freezing conditions (T2: -4°C) compared with control (CK: 16°C). The number of DEGs associated with two cultivars at two low temperature treatments (T1: 4°C and T2: -4°C) were 834, 1,353, 231, and 1,882 in four comparison groups (Xinmai26-CK vs. Xinmai26-T1, Xinmai26-CK vs. Xinmai26-T2, Yannong19-CK vs. Yannong19-T1, and Yannong19-CK vs. Yannong19-T2), respectively. Furthermore, to validate the accuracy of RNA-seq, 16 DEGs were analyzed using quantitative real-time RT-PCR. Several transcriptome changes were observed through Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway functional enrichment analysis in plant hormone signal transduction, circadian rhythm-plant, and starch and sucrose metabolism under low temperature. In addition, 126 transcription factors (TFs), including AP2-ERF, bHLH, WRKY, MYB, HSF, and members of the bZIP family, were considered as cold-responsive. It is the first study to investigate DEGs associated with low temperature stress at the transcriptome level in two wheat cultivars with different cold resistance capacities. Most likely, the variations in transcription factors (TFs) regulation, and starch and sucrose metabolism contribute to different cold resistance capacities in the two cultivars. Further, physiological activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzymes, malondialdehyde (MDA), soluble sugar (SS), and sucrose contents were evaluated to investigate the negative impacts of low temperature in both cultivars. These findings provide new insight into the molecular mechanisms of plant responses to low temperature and potential candidate genes that required for improving wheat's capacity to withstand low temperature stress.

Effects of Low Temperature Stress on Source-Sink Organs in Wheat and Phosphorus Mitigation Strategies.

The 21st century presents many challenges to mankind, including climate change, fast growing human population, and serious concerns over food security. Wheat is a leading cereal crop that largely fulfills the global food needs. Low temperature stress accompanied by nutrient-starved soils is badly disrupting the source-sink relationship of wheat, thus causing an acute decline in final yield and deteriorating the grain quality. This review paper aimed to understand how low temperature stress affects wheat source-sink organs (i.e., leaves, roots, and spikes) and how phosphorus application reliefs in alleviating its harmful consequences. Also, we discussed mitigation strategies to enhance wheat capacity to adapt to varying temperature extremes and made rational recommendations based on modern agronomic and breeding approaches. Therefore, this study is likely to establish a solid foundation for improving the tolerance to low temperature stress and to improve its phosphorus utilization efficiency in wheat.

Rapid assembly of colorless antimicrobial and anti-odor coatings from polyphenols and silver.

The development of antimicrobial fabrics and textiles that can sustainably inhibit a broad spectrum of microbes is crucial for protecting against pathogens in various environments. However, engineering antimicrobial textiles is challenging due to issues with discoloration and inhibited breathability, the use of harmful or harsh reagents and synthesis conditions, and complex and/or time-consuming processing. Herein, we develop a facile and rapid approach to deposit antimicrobial coatings using universally adherent plant polyphenols and antimicrobial silver ions. Importantly, the coatings are colorless, thin (< 10 nm), rapidly assembled (< 20 min), and can be deposited via immersion or spraying. We demonstrate that these metal-phenolic coatings on textiles can inhibit lipid-enveloped viruses over one thousand times more efficiently than coatings composed of other metal ions, while maintaining their efficacy even after 5 washes. Moreover, the coatings also inhibit Gram positive and negative bacteria, and fungi, and can prevent odors on clothes for at least 10 washes. Collectively, the ease of synthesis, use of simple and safe precursors, and amenability to at-home and industrial application suggests that the coatings will find practical application in various settings.

Effects of Low-Temperature Stress during the Anther Differentiation Period on Winter Wheat Photosynthetic Performance and Spike-Setting Characteristics.

Climate change has caused frequent extreme low-temperature events to threaten global food security. Spring low-temperature stress is one of the major limiting factors for high and stable yields of wheat. We used two wheat varieties differing in spring cold-sensitivity (cold-tolerant variety Yannong 19 and cold-sensitive variety Xinmai 26) to examine the effects of low-temperature stress during the anther differentiation period on wheat photosynthetic performance and spike-setting characteristics. Low-temperature stress was simulated in a climate box at -2 °C, 0 °C or 2 °C (night) and 15 °C (day) for 24 h, 48 h or 72 h. With the extension of the treatment time and the decrease of temperature, the photosynthetic rate, stomatal conductance and transpiration rate of wheat leaves gradually decreased. All treatments except -2 °C for 72 h recovered slowly within 7-15 days after treatment. Low-temperature stress greatly reduced grains per spikelet, 1000-grain weight and yield per plant. By analyzing the spikelets in different stalk locations (upper, middle and lower), we found that the number of upper spikelets was significantly less than lower and middle spikelets after low-temperature stress. The sterile grain of upper spikelets (Xinmai 26, for example) can reach 100% at -2 °C for 48 h and 72 h, and the yield loss rate was 90.52% at 2 °C for 24 h, which was much higher than for the lower spikelets (60.73%) and middle spikelets (50.94%). Overall, these findings suggest that low-temperature stress during the anther differentiation period alters the photosynthetic activity involved in the accumulation of dry matter in wheat, which leads to delaying young spike growth, especially for upper spikelets, and ultimately in a decrease in yield.

Synthesis of Dithiocatechol-Pendant Polymers.

Although the synthesis of thiophenol-pendant polymers was reported in the 1950s, the polymers generally suffered from oxidation and became insoluble in organic solvents, hampering detailed characterization and further applications. Dithiocatechol-pendant polymers, which have one additional ortho-thiol group than thiophenol-pendant polymers, have never been synthesized, despite their promise in various applications due to their analogous molecular structure with catechol-pendant polymers. Herein, we report the first synthesis of dithiocatechol-pendant polymers using a novel protection-deprotection strategy. We carefully examined the synthetic routes and identified the deprotection conditions that do not cause cross-linking of the dithiocatechol moieties. Because the resulting dithiocatechol-pendant polymers were soluble in common organic solvents (e.g., tetrahydrofuran and N,N-dimethylformamide), the polymers can be fully characterized by standard spectroscopic methods, providing valuable data for future researchers. We also showed that besides free-radical polymerization, reversible addition-fragmentation chain-transfer polymerization can also be adopted to synthesize dithiocatechol-pendant polymers. This work paves the way for the exploitation of dithiocatechol-containing polymers for the fabrication of novel functional materials.

[Current status and research advances of late spring coldness in wheat]. / å°éº¦å€’æ˜¥å¯’ç ”ç©¶çŽ°çŠ¶ä¸Žè¿›å±•.

Low temperature disasters in wheat occur frequently in recent years, due to global warming. Late spring coldness in wheat during jointing-booting stage is one of the important factors limiting the yield and quality of wheat. Here, we summarized the occurrence characteristics (identification, classification, and space-time characteristics) of late spring coldness in wheat, and the effects of late spring coldness on physiological characteristics (leaf, stem, spike, and root), yield and quality of wheat. Furthermore, we reviewed the research progresses on the breeding of late spring coldness tolerant wheat, molecular biology mechanism, the monitoring, early warning and risk assessment of late spring coldness. Finally, future research work on genetic basis of wheat resistance to late spring coldness, evaluation system for wheat damaged by late spring coldness, prevention and control technology system were prospected to provide a theoretical basis for genetic improvement and new cultivation regulation measures of the resistance of wheat varieties to late spring coldness.

Synthesis, antitumor and DNA cleavage activities of a novel class of dehydroabietylamine derivatives.

Previous studies have reported higher biological activity of dehydrorosinamine derivatives. In order to further synthesize novel compounds with higher biological activity, a series of novel compounds containing benzo-azepine structures were synthesized from dehydroabietylamine in good yields in this study. The structures of synthesized compounds were identified by infra red (IR), 1H-NMR, 13C-NMR, and mass spectra (MS) analysis. The antitumor activities of the target compounds against L02 and HepG2 cells were studied. Furthermore, the dehydroabietylamine derivatives were studied on plasmid DNA cleavage activities. The results showed that the synthesized target compound exhibit antitumor and DNA cleavage activities against plasmid DNA (Escherichia coli). Our results further demonstrate the relationship between the chemical structure and biological function of the synthesized compounds.

Modeling Maize Canopy Morphology in Response to Increased Plant Density.

Increased plant density markedly affects canopy morphophysiological activities and crop productivity. This study aims to model maize canopy final morphology under increased interplant competition by revising a functional-structural plant model, i.e., ADEL-Maize. A 2-year field experiment was conducted at Mengcheng, Anhui Province, China, in 2016 and 2018. A randomized complete block design of five plant densities (PDs), i.e., 4.5, 6, 7.5, 9, and 15 plants m-2, with three replications was applied using a hybrid, i.e., Zhengdan 958. Canopy morphology at different PDs was measured with destructive samplings when maize canopy was fully expanded. The relationship of changes of organ morphology in relation to increased plant density was analyzed based on 2016 data. The ADEL-Maize was first calibrated for the hybrid at 4.5 plants m-2 and then revised by introducing relationships identified from 2016 data, followed by independent validation with 2018 field data. A heatmap visualization was shown to clearly illustrate the effects of increased plant density on final morphology of laminae, sheaths, and internodes. The logarithmic + linear equations were found to fit changes for the organ size versus increased plant density for phytomers excluding ear position or linear equations for the phytomer at ear position based on 2016 field data. The revision was then further tested independently by having achieved satisfactory agreements between the simulations and observations in canopy size under different PDs with 2018 field data. In conclusion, this study has characterized the relationship between canopy morphology and increased interplant competition for use in the ADEL-Maize and realized the simulations of final size of laminae, sheaths, and internodes, as affected by increased plant density, laying a foundation to test an ideotype for maize withstanding high interplant competition.

Effect of extruded adzuki bean flour on the quality and α-glucosidase inhibitory activity of Chinese steamed bread.

In order to improve the commercial and nutritional quality of Chinese steamed bread (CSB), effect of extruded adzuki bean flour (EABF) substitution (10%, 20%, 30%, and 40%) for wheat flour on the quality properties of blended flour and CSB was studied. Results showed that the development time, stability time, and farinograph quality number of blended flours were decreased with EABF addition, although water absorption and softening degree were increased. Pasting properties of them were also degraded with the addition. EABF substitution lowered the lightness and strengthened the hardness of CSB. The sensory evaluation total scores of CSB were both improved with 10% and 20% EABF addition, because slight red color of them was favored by the panelist. Nutritional analysis showed that CSB with 10% and 20% EABF ranked higher protein and essential amino acids content than that of WF-CSB, especially lysine and threonine. Addition of EABF contributed to the superior α-glucosidase inhibitory activity of protein isolated from CSBs. α-Glucosidase inhibition rate of CSB made from EABF (39.88%) was improved nearly five times compared with that of WF. It might be concluded that CSB with addition of 10% and 20% EABF could be used as a nutritional and healthy staple food.

Optimal nitrogen regimes compensate for the impacts of seedlings subjected to waterlogging stress in summer maize.

A field experiment was performed to explore the compensation effects of different nitrogen (N) regimes on the growth and photosynthetic capacity in different leaf layers of the summer maize hybrid of LuYu9105 under waterlogging at the seedling stage. The results showed that waterlogging significantly decreased the maximum green leaf area (gLA) by 10.0~15.3% and 9.3~22.5%, mainly due to the reduction in the below-ear layer leaves at the silking stage in 2014 and 2015, respectively. Waterlogging also significantly decreased the ear leaf photosynthetic rate (PN), and Fv/Fm, Fv/Fo, ΦPSII and qP at the below-ear layer leaves at the mid- and late-filling stages, which was accompanied by a reduction in the duration of grain-filling (T) by 2.6~5.9%, thus resulting in a loss of grain yield by 7.0~18.5%. Interestingly, a shift in N from basal application to topdressing at the big flare stage was shown to compensate the adverse effects of waterlogging by through increased gLA and leaf photosynthetic capacity at the ear layer and the above-ear layer, as well as a greater grain-filling rate, resulting in an increase in grain yield by 9.9~27.0% and 17.8~25.8% compared to other N treatments. Therefore, this study showed that optimal nitrogen regimes during maize growth are capable of compensating for the impacts caused by waterlogging at the seedling stage.

What is in a name?: The development of cross-cultural differences in referential intuitions.

Past work has shown systematic differences between Easterners' and Westerners' intuitions about the reference of proper names. Understanding when these differences emerge in development will help us understand their origins. In the present study, we investigate the referential intuitions of English- and Chinese-speaking children and adults in the U.S. and China. Using a truth-value judgment task modeled on Kripke's classic Gödel case, we find that the cross-cultural differences are already in place at age seven. Thus, these differences cannot be attributed to later education or enculturation. Instead, they must stem from differences that are present in early childhood. We consider alternate theories of reference that are compatible with these findings and discuss the possibility that the cross-cultural differences reflect differences in perspective-taking strategies.

Magnetic anisotropy and high-frequency property of flexible FeCoTa films obliquely deposited on a wrinkled topography.

We investigated the magnetic anisotropy and the high-frequency property of flexible Fe60Co26Ta14 (FeCoTa) thin films obtained by oblique sputtering onto a wrinkled surface. The sinuously wrinkled topography is produced by growing Ta layer on a pre-strained polydimethylsiloxane (PDMS) membrane. Due to the enhanced effect of shadowing, the oblique deposition of FeCoTa layer gives rise to a shift of wrinkle peak towards the incident atomic flux. With increasing the PDMS pre-strain or increasing the oblique sputtering angle, both the uniaxial magnetic anisotropy and the ferromagnetic resonance frequency of FeCoTa films are enhanced, but the initial permeability decreases. The magnetization reversal mechanism of wrinkled FeCoTa films can be interpreted by a two-phase model composed of both coherent rotation and domain wall nucleation. With the enhancement of uniaxial magnetic anisotropy, the domain wall nucleation becomes pronounced in FeCoTa films.