Prevalence, outcomes and associated factors of SARS-CoV-2 infection in psoriasis patients of Southwest China: a cross-sectional survey.

In this study we aimed to investigate the prevalence of SARS-CoV-2 infection in psoriasis patients, and outcomes of SARS-CoV-2 infection and associated risk factors. A cross-sectional survey was conducted from February 2023 to March 2023. Information was obtained with online questionnaire about psoriasis patients on demographic characteristics, clinical characteristics, SARS-CoV-2 infection and outcomes, vaccination, and routine protection against COVID-19. Logistic regression analysis was used to explore risk factors with SARS-CoV-2 infection and exacerbation of psoriasis. A total of 613 participants were recruited. 516 (84.2%) were infected, and associated factors were sex, working status, routine protection against COVID-19, COVID-19 vaccination, impaired nail, infection exacerbate psoriasis, and severity of psoriasis. Among the patients infected with SARS-CoV-2, 30 (5.8%) required hospitalization, 122 (23.6%) had psoriasis exacerbation due to SARS-CoV-2 infection, and associated factors were subtype of psoriasis, discontinuation of psoriasis treatment during SARS-CoV-2 infection, response following COVID-19 vaccination, and severity of psoriasis. Booster dose vaccination contributed a low probability of COVID-19 sequelae. COVID-19 vaccine's effectiveness was unsatisfactory, while booster dose vaccination reduced the occurrence of COVID-19 sequelae in psoriasis patients of Southwest China. Patients treated with psoriasis shown to be safe, without a higher incidence of SARS-CoV-2 infection or COVID-19hospitalization compared to untreated patients. Stopping treatment during SARS-CoV-2 infection led to psoriasis exacerbation, so psoriasis treatment could be continued except severe adverse reaction.

An antimicrobial peptide with an aggregation-induced emission (AIE) luminogen for studying bacterial membrane interactions and antibacterial actions.

A fluorescence technique to investigate the interactions between bacterial membranes and an aggregation-induced emission (AIE) luminogen-decorated AMP (TPE-AMP) was reported. Our simple and fast method consists of mixing TPE-AMP and bacterial suspensions and recording the fluorescence signals by flow cytometry and confocal microscopy in a "non-washing" manner.

PEG-based hydrogels prepared by catalyst-free thiol-yne addition and their post-antibacterial modification.

PEG-based hydrogels possess tissue-like mechanical elasticity, solute permeability, cytocompatibility and biocompatibility. In this work, PEG-based hydrogels were prepared via nucleophilic thiol-yne addition between a 4-arm PEG functionalized with thiols (PEG10k-4-SH) and an electron-deficient alkyne (PEG10k-4-PP). The as-fabricated hydrogels still possess residual functionalities, enabling a second nucleophilic thiol-yne addition on the gel matrix. A thiol-containing fluorescent dye was conjugated with the electron-deficient alkyne appended hydrogels. A thiol-containing antimicrobial peptide (AMP-SH) was also embedded into the gel matrix via nucleophilic thiol-yne addition. The inhibition of bacterial growth in suspensions and contaminated substrate surfaces by the AMP-embedded hydrogels was studied. The cytotoxicity of unmodified and AMP-embedded PEG-based hydrogels against 3T3 fibroblasts was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) viability assay.

Breast cancer molecular subtypes of Uygur and Han in Xinjiang of China.

PURPOSE: To compare the difference between Uygur and Han patients with breast cancer in molecular subtype. METHODS: 4 immunohistochemical (IHC) markers (ER, PR, HER-2 and KI-67) were used to divide Uygur and Han breast cancer patients into 4 subtypes (Luminal A, Luminal B, HER-2 over expression and Basal-like), respectively. statistical analysis were used to evaluate difference in molecular subtype characteristics by race, tumor size, age of onset, menstruation and birth status, histological grade and lymph node metastasis. RESULTS: There is no statistical difference on the molecular subtypes between Han and Uygur. But some characteristics about four subtypes between Han and Uygur have statistical difference like age onset of the Her-2 overexpression cases, subtypes of age less than 35 years, menarche age of the Basal-like cases and tumor size of the Luminal A cases. Between Han and Uygur there is statistical difference on the menarche age, number of childbirths, and tumor size. The HER-2 overexpression and Basal-like subtypes were more likely to be grade III tumors both of Han and Uygur. Between 4 molecular subtypes of Han there have statistical difference in number of metastasis lymph nodes. CONCLUSIONS: Our result shows that there are some significant differences between Uygur and Han in the pathological features as well as molecular subtypes. Correct understanding the difference of breast cancer between Uygur and Han can provide guidance for clinical practice.

[Clinical and pathologic analysis of astrocytic tumors with loss of heterozygosity on chromosome 10q].

OBJECTIVE: To study the correlation between loss of heterozygosity (LOH) on chromosome 10q and pathologic features, pathogenesis, prognosis of astrocytic tumors. METHODS: LOH on 10q was studied by interphase fluorescence in-situ hybridization (FISH) in 85 cases of astrocytic tumor, including 35 cases of WHO grade II tumors and 50 cases of WHO grade IV tumors. RESULTS: LOH on 10q was detected in 6 cases (17.1%) of diffuse astrocytoma (WHO grade II) and 34 cases (68.0%) of glioblastoma (WHO grade IV). 10q polysomy was detected in 7 cases (20.0%) of diffuse astrocytoma and 11 cases (22.0%) of glioblastoma. The rates of LOH on 10q in young age group and elderly group were 36.4% (12/33) and 82.4% (28/34), respectively. The difference was of statistical significance (P < 0.05). The rates of LOH on 10q in the diffuse astrocytoma and glioblastoma were 21.4% (6/28) and 87.2% (34/39), respectively. The difference was also statistically significant (P < 0.05). Univariate survival analysis showed that patient age, pathologic grade and 10q on LOH correlated with duration of survival (P < 0.05). CONCLUSIONS: There are correlation between 10q LOH, patient age and pathologic grade of astrocytic tumors. LOH on 10q is also related to the pathogenesis of astrocytic tumors and is helpful in predicting prognosis.

[Blastic plasmacytoid dendritic cell neoplasm: a clinicopathologic study].

OBJECTIVE: To study the clinicopathologic features and differential diagnosis of blastic plasmacytoid dendritic cell neoplasm. METHODS: The clinical, morphology and immunophenotypic features were analyzed in 3 cases of blastic plasmacytoid dendritic cell neoplasm, with review of literature. RESULTS: The pathologic changes of these tumors accorded with that of blastic plasmacytoid dendritic cell neoplasm, and they also had new characteristics, including lineage other than T, B, myeloid and NK cells, and immunophenotypes of CD56(+) CD4(-) CD123(+) TdT(+) CD43(+) CD68(+) , CD56(+) CD4(+) CD123(-) TdT(+) CD43(+) CD68(-) and CD56(+) CD4(+) CD123(-/+) TdT(-) CD43(+) CD68(+) in the 3 cases, respectively. Bone marrow involvement was found 5 years later in case 1, and was then stable after chemotherapy; case 2 and case 3 were died 5 and 2 months after diagnosis, respectively. CONCLUSION: Blastic plasmacytoid dendritic cell neoplasm is a heterogeneous group of lymphoproliferative disorders, with different clinical, morphologic and immunophenotypic features.

[Clinicopathologic significance of ß-catenin protein and loss of heterozygosity on 10q in medulloblastoma].

OBJECTIVE: To study the expression of ß-catenin protein and the status of loss of heterozygosity (LOH) on chromsome 10q in medulloblastoma, with clinical correlation. METHODS: Immunohistochemical study for ß-catenin protein was carried out in 50 cases of medulloblastoma encountered in the First Affiliated Hospital of Xinjiang Medical University during the period from 2002 to 2011, including 32 cases of classic medulloblastoma, 13 cases of desmoplastic medulloblastoma and 5 cases of medulloblastoma with extensive nodularity. The status of LOH on 10q was also detected by fluorescence in-situ hybridization. The clinicopathologic characteristics and prognostic parameters were studied by Kaplan-Meien and Cox analysis. RESULTS: The rates of expression of ß-catenin protein in classic medulloblastoma, desmoplastic medulloblastoma and medulloblastoma with extensive nodularity were 53.1% (17/32), 4/13 and 1/5, respectively. The rate of LOH on 10q was 33.3% (8/24) in classic medulloblastoma and 2/11 in desmoplastic medulloblastoma. There was no statistically significant difference between the two tumor types. Univariate analysis showed that the expression of ß-catenin protein (P = 0.022), lack of LOH on 10q (P = 0.020), extensiveness of tumor resection (P < 0.01), radiotherapy (P = 0.002) and chemotherapy (P < 0.01) represented important prognostic factors. CONCLUSIONS: Medulloblastoma with expression of ß-catenin protein and without LOH on 10q carries a better prognosis. Assessment of these parameters is helpful in evaluating prognosis and subsequent patient management.

[Making of compound Rhizoma Acori Tatarinowii naphtha emulsion].

OBJECTIVE: To determine an ideal recipe and technique for making of compound Rhizoma Acori Tatarinowii naphtha emulsion (CRATNE) , diameter for the granule in 10 to approximately 20 microm. METHODS: CRATNE was made by two-step emulsification and the recipe was evaluated to investigate the effect of diameter for the granule on stir time and stir speed and oxygen ventilated. RESULTS: The percentages of diameter for the granule in 10 to approximately 20 microm was 72%. Emulsion was made on recipe No. 4 [ Rhizoma Acori Tatarimowii naphtha (ml): liquid paraffin (ml): Sorbian monooleate (g): 0.5% gelatin solution (ml): distilled water (ml) is 1.05: 16: 7: 1: 16] and compound emulsion is made on recipe No. 4 [fore emulsion (g): Tween-80 (g): distilled water (ml) is 10: 1: 9]. Recipe No. 4 was the best one if stiring at low speed through magnetic force mixer was used. Stir time was 1 min. The oxygen was ventilated with a flow rate of 2 L/min for CRATNE. Ventilation time was 1 h. The percentages of diameter for the granule in 10 to approximately 12 microm increased from 72% to 79%. was type W/O/W. CONCLUSION: The diameter of CRATNE for the granule is in 10 to approximately 20 microm, can be made following above-mentioned methods.

Evidence for alternative candidate genes near RB1 involved in clonal expansion of in situ urothelial neoplasia.

In this paper, we present whole-organ histologic and genetic mapping studies using hypervariable DNA markers on chromosome 13 and then integrate the recombination- and single-nucleotide polymorphic sites (SNPs)-based deletion maps with the annotated genome sequence. Using bladders resected from patients with invasive urothelial carcinoma, we studied allelic patterns of 40 microsatellite markers mapping to all regions of chromosome 13 and 79 SNPs located within the 13q14 region containing the RB1 gene. A whole-organ histologic and genetic mapping strategy was used to identify the evolution of allelic losses on chromosome 13 during the progression of bladder neoplasia. Markers mapping to chromosomal regions involved in clonal expansion of preneoplastic intraurothelial lesions were subsequently tested in 25 tumors and 21 voided urine samples of patients with bladder cancer. Four clusters of allelic losses mapping to distinct regions of chromosome 13 were identified. Markers mapping to the 13q14 region that is flanked by D13S263 and D13S276, which contains the RB1 gene, showed allelic losses associated with early clonal expansion of intraurothelial neoplasia. Such losses could be identified in approximately 32% bladder tumor tissue samples and 38% of voided urines from patients with bladder cancer. The integration of distribution patterns of clonal allelic losses revealed by the microsatellite markers with those obtained by genotyping of SNPs disclosed that the loss within an approximately 4-Mb segment centered around RB1 may represent an incipient event in bladder neoplasia. However, the inactivation of RB1 occurred later and was associated with the onset of severe dysplasia/carcinoma in situ. Our studies provide evidence for the presence of critical alternative candidate genes mapping to the 13q14 region that are involved in clonal expansion of neoplasia within the bladder antecedent to the inactivation of the RB1 gene.