Waardenburg syndrome type 2 with a de novo variant of the SOX10 gene: a case report.

BACKGROUND: Waardenburg syndrome type 2 (WS2) has been reported to be a rare hereditary disorder, which is distinguished by vivid blue eyes, varying degrees of hearing impairment, and abnormal pigment deposition in the skin and hair. Variants in the sex-determining region Y-box containing gene 10 (SOXl0) gene may cause congenital deafness and have been demonstrated to be important during the development of WS2. METHODS: Complete clinical data of the proband and her family members (her parents and 2 sisters) was collected and physical examinations were performed in the hospital. The laboratory examination including hemoglobin, Coomb's test, urine protein, ENA, autoimmune hepatitis-related autoantibodies and ultrasonography were all conducted. We obtained the peripheral blood samples from all the participants and performed whole exome sequencing and sanger sequencing validation. RESULTS: The present study identified a family of 5 members, and only the proband exhibited typical WS2. Beyond the characteristics of WS2, the proband also manifested absence of puberty. The proband and her younger sister manifested systemic lupus erythematosus (SLE). Whole exome sequencing revealed a de novo variant in the SOX10 gene. The variant c.175 C > T was located in exon 2 of the SOX10 gene, which is anticipated to result in early termination of protein translation. CONCLUSION: The present study is the first to report a case of both WS2 and SLE, and the present findings may provide a new insight into WS2.

Lysimachia capillipes Hemsl. saponins ameliorate colorectal cancer in mice via regulating gut microbiota and restoring metabolic profiles.

Lysimachia capillipes Hemsl., a traditional Chinese medicine (TCM), is commonly prescribed for its anti-inflammatory and anti-tumor properties. Pharmacological studies have demonstrated that Lysimachia capillipes Hemsl. saponins (LCS) are the primary bioactive component. However, its mechanism for treating colorectal cancer (CRC) is still unknown. Increasing evidence suggests a close relationship between CRC, intestinal flora, and host metabolism. Thus, this study aims to investigate the mechanism of LCS amelioration of CRC from the perspective of the gut microbiome and metabolome. As a result, seven gut microbiotas and fourteen plasma metabolites were significantly altered between the control and model groups. Among them, one gut microbiota genera (Monoglobus) and six metabolites (Ureidopropionic acid, Cytosine, L-Proline, 3-hydroxyanthranilic acid, Cyclic AMP and Suberic acid) showed the most pronounced callback trend after LCS administration. Subsequently, the correlation analysis revealed significant associations between 68 pairs of associated metabolites and gut microbes, with 13 pairs of strongly associated metabolites regulated by the LCS. Taken together, these findings indicate that the amelioration of CRC by LCS is connected to the regulation of intestinal flora and the recasting of metabolic abnormalities. These insights highlight the potential of LCS as a candidate drug for the treatment of CRC.

Moving stimuli enhance beat timing and sensorimotor coupling in vision.

Vision has long been known for its inefficiency in beat perception and synchronization. However, this has been challenged by the finding that moving stimuli (bouncing ball or moving bar) can significantly improve visual beat synchronization. The present study examined two possible mechanisms for this phenomenon: visual motion facilitates temporal processing or promotes sensorimotor coupling. Instead of a single visual object (such as a ball or bar), random-dot kinematograms (RDKs) were used to construct visual motion sequences to avoid confounding factors, such as changes in trajectory and velocity. Experiment 1 showed that RDKs improved beat-timing discrimination compared with visual flashes, but auditory tones were still superior to RDKs. In Experiment 2, synchronized movements improved auditory-tone beat timing but impaired visual-flash beat timing, with no effect on RDK beat timing. Experiment 3 indicated that the regression slope of the phase correction response in RDKs was higher than that in visual flashes but still lower than that in auditory tones. The results showed that moving stimuli enhances both temporal processing (Experiment 1) and sensorimotor coupling (Experiments 2 and 3) in vision, but to a lesser degree, with audition retaining an advantage. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Cognitive style of field dependence-independence modulates the working memory storage of biological motion.

The biological motion refers to the continuous configuration movement of live agents in space. The perceptual processing of biological motion has the specificity of the dissociation between body form and body motion. However, there is limited evidence for whether such specificity continues when holding biological motion in working memory. We explored this question from the perspective of field dependence (FD) and field independence (FI) cognitive styles in the current study. Three categories of biological motion have been developed: intact movement, motion feature, and form feature. We examined the working memory capacity of motion features, form features, intact movements (Experiments 1-3), and the recognition of three categories of biological motion when remembering intact movements (Experiment 4). The results showed that for the motion features, FI individuals had better memory performance when remembering five items and showed greater working memory capacity and recognition compared with FD individuals, whereas the opposite pattern was observed between FI and FD individuals for the form features. The cognitive style could modulate the working memory storage of biological motion when the task becomes demanding, suggesting that body form and body motion are dissociable in working memory. Our study provided additional evidence for the specificity of biological motion processing in working memory, extending the hierarchical neural model. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Neural manifestation of L2 novel concept acquisition from multi-contexts via both episodic memory and semantic memory systems.

This study aims to examine the process of L2 novel word learning through the combination of episodic and semantic memory, and how the process differs between the formation of thematic and taxonomic relations. The major approach adopted was observing the neural effects of word learning, which is manifested in the N400 from event-related potentials (ERPs). Eighty-eight participants were recruited for the experiment. In the learning session, L2 contextual discourses related to novel words were learned by participants. In the testing session, discourses embedded with incongruous and congruous novel words in the final position were used for participants to judge the congruency which affected the N400 neural activity. The results showed that both recurrent and new-theme discourses elicited significant N400 effects, while taxonomic sentences did not. These results confirmed the formation of episodic and semantic memory during L2 new word learning, in which semantic memory was mainly supported by thematic relations.

The Reactivation of working memory representations affects attentional guidance.

Recent studies have suggested that the neural activity that supported working memory (WM) storage is dynamic over time and this dynamic storage decides memory performance. Does the temporal dynamic of the WM representation also affect visual search, and how does it interact with distractor suppression over time? To address these issues, we tracked the time course of the reactivation of WM representations during visual search by analyzing the electroencephalogram (EEG) and event-related optical signals (EROS) in Experiments 1 and 2, respectively, and investigated the interaction between the representation reactivation and distractor suppression in Experiment 3. Participants had to maintain a color in WM under high- or low-precision requirement and perform a subsequent search task. The reactivation of WM representations was defined by the above-chance decoding accuracy. The EEG results showed that compared with the low-precision requirement, WM-matching distractors captured more attention and the WM representation were reactivated more frequently under high-precision requirement. The EROS results showed that compared with the low-precision requirement, the increased activity in occipital cortex in the WM-matching versus WM-mismatching conditions was observed at 224 ms during visual search under high-precision requirement. Regression analysis showed that the representation reactivation during visual search directly predicted the behavioral WM-based attentional capture effect, while the representation reactivation before visual search impacted the WM-based attentional capture effect through the mediation of distractor suppression during visual search. These results suggest that the reactivation of WM representations and distractor suppression collectively determine WM-based attentional capture.

Deciphering the metabolic profile and anti-colorectal cancer mechanism of Capilliposide A using ultra performance liquid chromatography mass spectrometry combined with non-targeted metabolomics studies.

Colorectal cancer is a highly prevalent malignancy that threatens human health worldwide. Despite the availability of chemotherapy as a primary treatment option, individuals with CRC undergoing frequent chemotherapy are susceptible to developing drug resistance, which can result in poor treatment outcomes. Consequently, there is an urgent need to discover new bioactive compounds for the treatment of CRC. Capilliposide A is a triterpenoid saponin that is extracted from Lysimachia capillipes Hemsl. Although it has been reported that LC-A exhibits good bioactivity, its metabolic profile and potential mechanism underlying its anti-CRC effects remain unknown. In this study, the metabolic products of LC-A in rat plasma, feces, and urine were identified using an LC-MS platform. In addition, LC-MS-based metabolomics was employed to investigate the mechanism of LC-A against CRC. The results showed that LC-A significantly inhibited CRC cell proliferation, attenuated tumor growth, and alleviated metabolic abnormalities in CRC-bearing mice. Furthermore, the levels of p-cresol sulfate and phenylacetylglycine in CRC model plasma decreased, with an increment in sphingosine 1-phosphate, D-tryptophan, and L-2-aminoadipic acid. These metabolite levels can be reversed by LC-A treatment. These metabolite alterations were related to the sphingolipid and amino acid metabolic pathways, demonstrating that LC-A anti-CRC effects were regulated through the modulation of underlying metabolism. Additionally, seven metabolites of LC-A were characterized in rat feces, plasma, and urine. This study offers a scientific foundation for elucidating the metabolism of LC-A and its treatment of colorectal cancer.

Capilliposide B inhibits the migration of prostate cancer by inducing autophagy through the ROS/AMPK/mTOR pathway.

Capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, is a potent anticancer agent. However, its anticancer mechanism remains elusive. In the present study, we demonstrated the potent anti-tumor activity and molecular mechanism of CPS-B both in vitro and in vivo. Proteomic analysis using isobaric tags for relative and absolute quantitation techniques suggested that CPS-B modulated autophagy in prostate cancer (PC). Moreover, Western blotting showed that both autophagy and epithelial-mesenchymal transition occurred place after CPS-B treatment in vivo, which was also proven in PC-3 cancer cells. We deduced that CPS-B inhibited migration by inducing autophagy. We examined the accumulation of reactive oxygen species (ROS) in cells, and in downstream pathways, LKB1 and AMPK were activated while mTOR was inhibited. Transwell experiment results showed that CPS-B inhibited the metastasis of PC-3 cells and that this effect was significantly attenuated after pretreatment with chloroquine, indicating that CPS-B inhibited metastasis via autophagy induction. Altogether, these data suggest that CPS-B is a potential therapeutic agent for cancer treatment that acts by inhibiting migration through the ROS/AMPK/mTOR signaling pathway.

Modulation of similarity on the distraction resistance of visual working memory representation.

Controversy exists regarding the distraction resistance of priority items in visual working memory (VWM). The protection account proposes that high-priority items resist distraction more than low-priority items, while the vulnerability account proposes that distractors impair high-priority items more. We proposed another available resource threshold account: distraction will not impair items if available resources exceed their threshold needed for sufficient processing. Using a change-detection paradigm, we manipulated item priority by color similarity and inserted distractors during VWM retention. We investigated the effect of similarity on distraction resistance of relevant (color) and irrelevant (shape) feature representations (Experiments 1-2), and the neural mechanism of this effect using event-related potentials (ERPs; Experiment 3). Behavioral results showed distractors impaired the accuracy of dissimilar items when relevant features were memorized and of similar items when irrelevant features were memorized under simultaneous presentation of similar and dissimilar items. Moreover, distractors impaired the accuracy of dissimilar items when relevant features were memorized and of both similar and dissimilar items when irrelevant features were memorized under separate presentation of items. ERP results showed a smaller negative slow wave and P2 but larger N2 under the similar condition. Similarity protected relevant features of similar items against distraction by reducing memory load, decreasing attentional resources allocated to distractors, and strengthening inhibition of distractors. However, similarity did not protect irrelevant features of similar items. Our results support the available resource threshold account, suggesting that VWM is a flexible and intelligent system despite its limited capacity.

Optimization of Thermo-Mechanical Fatigue Life for Eutectic Al-Si Alloy by the Ultrasonic Melt Treatment.

The eutectic cast Al-Si alloys with excellent high-temperature and casting performance are widely used in engine pistons. During frequent starts and stops, the thermo-mechanical fatigue (TMF) is the most important failure cause. Ultrasonic melt treatment (UT) was chosen to compare and investigate the influence of micro-structures on fatigue life and damage mechanisms of as-cast (AC) eutectic Al-Si alloys under TMF loading. After UT, the grain size, primary Si, and intermetallic particles are reduced significantly in the alloy; fatigue life increases obviously. As a result of pilling-up of dislocations, the competitive effects of the critical strain/stress for fatigue crack nucleation can be found. There are two different crack initiation mechanisms under TMF: one is primary Si fracture for AC alloys with limited critical strain/stress for fatigue crack nucleation at fractured Si particles, and the other is primary Si debonding for UT alloys with increasing critical fracture strain/stress. After the crack initiation, the fractured or debonded primary phases provide the advantages for the further development of main cracks for both alloys. The UT alloy (805 ± 253 cycles) has about twice the TMF life of the AC alloy (403 ± 98 cycles). The refinement of micro-structures is instrumental in improving the fatigue resistance and life of TMF for the UT alloy.

Guangsangon E triggers mitochondria dysfunction and mitophagy in triple-negative breast cancer and leads to non-apoptotic cell death.

Guangsangon E (GSE) is a natural product separated from Morus alba L. It has been reported to treat lung cancer through autophagy. However, whether GSE is effective in repressing triple-negative breast cancer (TNBC) cells is yet to be elucidated. In the present study, GSE inhibited cell growth of MDA-MB-231, MDA-MB-453, and MDA-MB-468 cells. Moreover, GSE induced mitochondrial dysfunction, including membrane potential loss, mitochondria fission, and reactive oxygen species accumulation, and finally led to mitophagy-related non-apoptotic cell death. In the xenograft tumor nude mice, GSE treatment significantly reduced the size and weight of MDA-MB-231 tumors. The tumor inhibition rates of GSE treatment were 49.68% (low-dose) and 48.73% (high-dose). In summary, GSE is a potential anticancer drug available for treating TNBC with apoptosis resistance.

Heavy metals intoxication in a patient with POEMS-like symptoms: a case report.

BACKGROUND: POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes) is a kind of plasma cell disease with complex clinical manifestations involving multiple systems. Metal poisonings through a mucocutaneous are rare in clinic and reported less in the literature. People may exposure to toxic metals through air, food, water, or inappropriate use of drugs. Acute or chronic poisonings can lead to various toxic effects on body tissues and organs. Both POEMS syndrome and heavy metal intoxication are uncommon with multifarious and nonspecific clinical manifestations. Here we describe a case of a 54-year-old man with polyarticular pain and IgA lambda type monoclonal protein in his serum. The diagnosis was confirmed by heavy metals testing in his urine and the herbal mixtures he took. This is the first available report of arsenic and mercury intoxication mimicking POEMS syndrome.

Identification of Potential Diagnoses Based on Immune Infiltration and Autophagy Characteristics in Major Depressive Disorder.

Background: Major depressive disorder (MDD) is a serious mental illness characterized by mood changes and high suicide rates. However, no studies are available to support a blood test method for MDD diagnosis. The objective of this research was to identify potential peripheral blood biomarkers for MDD and characterize the novel pathophysiology. Methods: We accessed whole blood microarray sequencing data for MDD and control samples from public databases. Biological functions were analysed by GO and KEGG pathway enrichment analyses using the clusterprofile R package. Infiltrated immune cell (IIC) proportions were identified using the CIBERSORT algorithm. Clustering was performed using the ConsensusClusterPlus R package. Protein-protein interactions (PPI) were assessed by constructing a PPI network using STRING and visualized using Cytoscape software. Rats were exposed to chronic unpredictable mild stress (CUMS) for 6 weeks to induce stress behaviour. Stress behaviour was evaluated by open field experiments and forced swimming tests. Flow cytometry was used to analyse the proportion of CD8+ T cells. The expression of the corresponding key genes was detected by qRT-PCR. Results: We divided MDD patients into CD8H and CD8L clusters. The functional enrichment of marker genes in the CD8H cluster indicated that autophagy-related terms and pathways were significantly enriched. Furthermore, we obtained 110 autophagy-related marker genes (ARMGs) in the CD8H cluster through intersection analysis. GO and KEGG analyses further showed that these ARMGs may regulate a variety of autophagy processes and be involved in the onset and advancement of MDD. Finally, 10 key ARMGs were identified through PPI analysis: RAB1A, GNAI3, VAMP7, RAB33B, MYC, LAMP2, RAB11A, HIF1A, KIF5B, and PTEN. In the CUMS model, flow cytometric analysis confirmed the above findings. qRT-PCR revealed significant decreases in the mRNA levels of Gnai3, Rab33b, Lamp2, and Kif5b in the CUMS groups. Conclusion: In this study, MDD was divided into two subtypes. We combined immune infiltrating CD8+ T cells with autophagy-related genes and screened a total of 10 ARMG genes. In particular, RAB1A, GNAI3, RAB33B, LAMP2, and KIF5B were first reported in MDD. These genes may offer new hope for the clinical diagnosis of MDD.

More Expensive, More Attractive? The Effect of Pricing on Gift Evaluation: Differences Between Giver and Receiver.

The present research explored differences in gift evaluation between gift givers and receivers. Three studies were conducted to test how the pricing influenced the gift evaluations of givers and receivers, and whether the price-quality and price-monetary sacrifice inferences were the underlying mechanisms. The results showed that: givers evaluated high-priced gifts as better than low-priced gifts, whereas receivers evaluated low-priced gifts as better than high-priced gifts; price-quality inference mediated the effect of pricing on gift evaluations, but only for givers. Furthermore, the effect of pricing on gift evaluation was moderated by the gift type: givers evaluated the high-priced gift as better only for the desirable gift (but not for the feasible gift); receivers evaluated the low-priced gift as better only for the feasible gift (but not for the desirable gift). The results demonstrate the effect of pricing on gift evaluation and could contribute to understanding the differences between givers' and receivers' perception of what a "good gift" is, and the underlying psychological mechanisms.

Decoding Color Visual Working Memory from EEG Signals Using Graph Convolutional Neural Networks.

Color has an important role in object recognition and visual working memory (VWM). Decoding color VWM in the human brain is helpful to understand the mechanism of visual cognitive process and evaluate memory ability. Recently, several studies showed that color could be decoded from scalp electroencephalogram (EEG) signals during the encoding stage of VWM, which process visible information with strong neural coding. Whether color could be decoded from other VWM processing stages, especially the maintaining stage which processes invisible information, is still unknown. Here, we constructed an EEG color graph convolutional network model (ECo-GCN) to decode colors during different VWM stages. Based on graph convolutional networks, ECo-GCN considers the graph structure of EEG signals and may be more efficient in color decoding. We found that (1) decoding accuracies for colors during the encoding, early, and late maintaining stages were 81.58%, 79.36%, and 77.06%, respectively, exceeding those during the pre-stimuli stage (67.34%), and (2) the decoding accuracy during maintaining stage could predict participants' memory performance. The results suggest that EEG signals during the maintaining stage may be more sensitive than behavioral measurement to predict the VWM performance of human, and ECo-GCN provides an effective approach to explore human cognitive function.

Mitochondrial proteomic analysis reveals the regulation of energy metabolism and reactive oxygen species production in Clematis terniflora DC. leaves under high-level UV-B radiation followed by dark treatment.

Clematis terniflora DC. is an important medicinal plant from the family Ranunculaceae. A previous study has shown that active ingredients in C. terniflora, such as flavonoids and coumarins, are increased under ultraviolet B radiation (UV-B) and dark treatment and that the numbers of genes related to the tricarboxylic acid cycle and mitochondrial electron transport chain (mETC) are changed. To uncover the mechanism of the response to UV-B radiation and dark treatment in C. terniflora, mitochondrial proteomics was performed. The results showed that proteins related to photorespiration, mitochondrial membrane permeability, the tricarboxylic acid cycle, and the mETC mainly showed differential expression profiles. Moreover, the increase in alternative oxidase indicated that another oxygen-consuming respiratory pathway in plant mitochondria was induced to minimize mitochondrial reactive oxygen species production. These results suggested that respiration and mitochondrial membrane permeability were deeply influenced to avoid energy consumption and maintain energy balance under UV-B radiation and dark treatment in C. terniflora leaf mitochondria. Furthermore, oxidative phosphorylation was able to regulate intracellular oxygen balance to resist oxidative stress. This study improves understanding of the function of mitochondria in response to UV-B radiation and dark treatment in C. terniflora. SIGNIFICANCE: C. terniflora was an important traditional Chinese medicine for anti-inflammatory. Previous study showed that the contents of coumarins which were the main active ingredient in C. terniflora were induced by UV-B radiation and dark treatment. In the present study, to uncover the regulatory mechanism of metabolic changes in C. terniflora, mitochondrial proteomics analysis of leaves was performed. The results showed that photorespiration and oxidative phosphorylation pathways were influenced under UV-B radiation and dark treatment. Mitochondria in C. terniflora leaf played a crucial role in energy mechanism and regulation of cellular oxidation-reduction to maintain cell homeostasis under UV-B radiation followed with dark treatment.

A two-step strategy for quality control of Xin-Yu-Tie-Pian, an ointment patch.

Xin-Yu-Tie-Pian, an ointment patch which is composed of Tetradium ruticarpum and Asarum sieboldii Miquel, can be used for curing recurrent oral ulcers owing to its good bioactivities. Currently, the lack of a method for its quality evaluation hinders the development and clinical application of Xin-Yu-Tie-Pian. Thus, it is necessary to perform research on quality control. The chromatographic fingerprint, as an identification method, and the simultaneous determination method for two bioactive constituents, evodiamine and rutecarpine, can be used to evaluate the quality of traditional medicine. In this study, a two-step strategy including fingerprint analysis for identification and a simultaneous determination method for two bioactive constituents was performed for Xin-Yu-Tie-Pian quality control. The fingerprint analysis was validated by stability, precision and repeatability tests and a similarity evaluation was performed with 10 selected characteristic fingerprint peaks of 10 batches of Xin-Yu-Tie-Pian patch. Meanwhile, the simultaneous determination method was evaluated by methodological experiments, including linearity, accuracy, repeatability, stability and feasibility. Finally, the results indicate that this two-step strategy, including HPLC fingerprint analysis and simultaneous determination method, can be successfully applied for the assessment of the quality and quantity of Xin-Yu-Tie-Pian.

Investigation of phytochemical composition and metabolite profiling in vivo of Beta vulgaris L.

RATIONALE: Beta vulgaris L. has attracted increasing attention because of its broad application. The root of B. vulgaris L. (beetroot) possesses many excellent biological properties such as antianemic, anti-inflammatory, antihypertensive, antioxidant, anticarcinogenic, antipyretic, antibacterial, detoxicant, and diuretic. The chemical constituents of beetroot play a major role in the research on beetroot application and development. At present, no systematic identification study that focuses on the chemical constituents of beetroot has been reported. METHODS: This study investigated a three-step strategy comprising phytochemical profiling, prototype profiling, and metabolism of its correlative metabolites in vivo using ultra-performance liquid chromatography tandem quadruple time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). RESULTS: UPLC-QTOF-MS/MS technique proved to be a rapid, sensitive, and reliable method for monitoring the specific ingredients as well as the whole chemical constituents in beetroot. In sum, 95 phytochemical compounds, 51 prototype compounds, and 37 derived metabolites in vivo were found in beetroot. CONCLUSIONS: The main metabolic pathways were sulfonation, glucuronidation, methylation/sulfonation, and methylation. The present findings provided the phytochemical basis both in vitro and in vivo for future application.

New Determination Method of Two Bioactive Saponins in Lysimachia capillipes Hemsl. by Quantitative Analysis of Multi-Components by Single-Marker.

Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.

DHOK Exerts Anti-Cancer Effect Through Autophagy Inhibition in Colorectal Cancer.

DHOK (14,15ß-dihydroxyklaineanone) is a novel diterpene isolated from roots of Eurycoma longifolia Jack, a traditional herb widely applied in Southeast Asia. It is reported that DHOK has cytotoxic effect on cancer cells, but its anti-cancer mechanism has still been not clear. In our study, we first observed that DHOK inhibits cell proliferation of colorectal cancer cells in a time- and dose-dependent manner. Next, we performed transcriptome sequencing to identify the targets of DHOK and found that autophagy-related signaling pathways are involved under DHOK treatment. Indeed, in DHOK-treated cells, the level of autophagosome marker LC3 and the formation of GFP-LC3 puncta were decreased, indicating the reduction of autophagy. Moreover, confocal microscopy results revealed the lysosomal activity and the formation of autolysosomes are also inhibited. Our western blotting results demonstrated the activation of mammalian target of rapamycin (mTOR) signaling pathway by DHOK, which may be attributed to the enhancement of ERK and AKT activity. Functionally, activation of autophagy attenuated DHOK-caused cell death, indicating that autophagy serves as cell survival. In xenograft mouse model, our results also showed that DHOK activates the mTOR signaling pathway, decreases autophagy level and inhibits the tumorigenesis of colon cancer. Taken together, we revealed the molecular mechanism of DHOK against cancer and our results also demonstrate great potential of DHOK in the treatment of colorectal cancer.