RESUMEN
OBJECTIVE: To analyse a special kind of Schisandra chinensis with the white fruit using ITS2 barcode at molecular levels. METHOD: ITS2 regions were sequenced bidirectionally. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner, MEGA 5.0 software was used to align the sequences. The ITS2 secondary structure was predicted using ITS2 web server, BLAST 1 method was used to identify the S. chinensis with the white fruit. RESULT: The length of the ITS2 sequence was 231 bp. And the sample was identified as S. chinensis using the method of BLAST 1. Their mean interspecific genetic distance (K2P distance) among the populations of the S. chinensis with the white fruit and S. chinensis was far lower than the mean interspecific genetic distance between the S. chinensis and S. sphenanthera. CONCLUSION: By using ITS2 the S. chinensis with the white fruit was identified as S. chinensis, and the ITS2 barcode could be used to identify S. chinensis and S. sphenanthera.
Asunto(s)
ADN de Plantas/genética , ADN Espaciador Ribosómico/genética , Schisandra/química , Schisandra/genética , ADN de Plantas/química , ADN Espaciador Ribosómico/química , Frutas/química , Frutas/clasificación , Frutas/genética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Schisandra/clasificación , Análisis de Secuencia de ADN , Programas InformáticosRESUMEN
OBJECTIVE: The characters of Schisandra chinensis with white fruit were represented at molecular levels and the genetic diversity were investigated using RAPD and ISSR. METHODS: 12 primers of RAPD randomized markers and 8 primers of ISSR markers were used to test 21 samples of white fruit Schisandra chinensis, and POPGENE 32 software were used to analyze the results. RESULTS: One or more unique bands were produced to distinguish white fruit Schisandra chinensis from normal Schisandra chinensis using the primers of S83, S180 and S300. RAPD:66 discernible DNA fragments were generated with 52 (78.79%) polymorphic fragments; ISSR: 42 discernible DNA fragments were generated with 25 (59.52%) polymorphic fragments. The genetic variation of white fruit Schisandra chinensis was more unstable than normal Schisandra chinensis, but the genetic distance of them was small at the species level. CONCLUSION: RAPD and ISSR markers can be used to put up the characteristics of Schisandra chinensis with white fruit at molecular levels. Also they can indicate the genetic relationship of the Schisandra chinensis germplasm resource.
Asunto(s)
Frutas/genética , Variación Genética , Repeticiones de Microsatélite/genética , Técnica del ADN Polimorfo Amplificado Aleatorio/métodos , Schisandra/genética , Cartilla de ADN/genética , ADN de Plantas/genética , Frutas/clasificación , Datos de Secuencia Molecular , Filogenia , Plantas Medicinales/clasificación , Plantas Medicinales/genética , Schisandra/clasificación , Análisis de Secuencia de ADNRESUMEN
OBJECTIVE: To establish an effective and convenient method for HPLC fingerprints of Xuezhiling tablets and new Xuezhiling tablets, and to observe the changes of fingerprint with crude and processed Cassiae Semen in Xuezhiling tablets. METHODS: The HPLC with Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) column was used for the gradient elution of acetonitrile-0.1% phosphoric acid solution, at the flow rate of 1.0 mL/min. Detection wavelength was set at 284 nm and the column temperature was 30 degrees C. RESULTS: Ten batches of Xuezhiling tablets and new Xuezhiling tablets were tested and gained HPLC fingerprint containing 20 common peaks, respectively. CONCLUSION: This method is stable and reliable. The number of common peaks of fingerprint had little change after the crude and processed Cassiae Semen in Xuezhiling tablets interchangeably, but the contents of some components had significant changes.
Asunto(s)
Antraquinonas/análisis , Cassia/química , Medicamentos Herbarios Chinos/química , Hipolipemiantes/química , Plantas Medicinales/química , Alisma/química , Cromatografía Líquida de Alta Presión , Combinación de Medicamentos , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/normas , Emodina/análisis , Hipolipemiantes/aislamiento & purificación , Hipolipemiantes/normas , Control de CalidadRESUMEN
The effect of plant growth regulator forchlorfenuron (CPPU) 1 x 10(-6), 0.67 x 10(-6), 0.5 x 10(-6) on fruit morphology and effective components lignans was studied. Those morphologies were the combination of four basic morphological changes. The result showed, diametre were increased and longitudinal diametre of fruits were inhibited by foliage fertilizers including CPPU. At the same time, 1 000-grain weight and yield showed the varying degrees increase under CPPU. The order of the degree was 0.5 x 10(-6) > 1 x 10(-6) > 0.67 x 10(-6). Six lignans content of Schisandra chinensis of different harvest time and different CPPU processing groups were determined, the results showed that lignans accumulation occurred mainly in periods of premature the half mature fruiting stages. Under the 0.67 x 10(-6) CPPU treatment, schisandrol B, schisandrin B, schisandrin C content of S. chinensis showed different increase.
Asunto(s)
Frutas/efectos de los fármacos , Lignanos/metabolismo , Compuestos de Fenilurea/farmacología , Piridinas/farmacología , Cromatografía Líquida de Alta Presión , Ciclooctanos/análisis , Ciclooctanos/metabolismo , Dioxoles/análisis , Dioxoles/metabolismo , Relación Dosis-Respuesta a Droga , Frutas/crecimiento & desarrollo , Frutas/metabolismo , Lignanos/análisis , Compuestos Policíclicos/análisis , Compuestos Policíclicos/metabolismoRESUMEN
OBJECTIVE: To compare the content of six lignans of different parts of Schisandra chinensis. METHOD: Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water gradient system as mobile phase. Wave length was 250 nm. The flow rate was 1 mL x min(-1). Column temperature was 30 degrees C. RESULT: The total lignans content of wild Schisandra chinensis was higher than that of the cultivated varieties. The total lignans content of different parts varied significantly, wherein the root > main branch > side branches > leaf. CONCLUSION: This method is stable, reliable, can be used for the quality evaluation of different parts of Schisandra.
