RESUMEN
BACKGROUND: Serum procalcitonin (PCT) has been recognized as a primary biomarker in bacterial infections, and monitoring its concentration could help to evaluate the prognosis of sepsis and guide the antibiotic administration. We aimed to establish a fast and accurate immunoassay for PCT quantitation. METHODS: Our newly developed monoclonal antibodies (mAbs) against human PCT were preliminarily evaluated by enzyme-linked immunosorbent assay and then used to develop a chemiluminescence enzyme immunoassay (CLEIA). The proposed CLEIA was assessed in analytical performance and applied to measurement of serum PCT. RESULTS: mAb 2D3 and mAb 8F6 were selected as capture and detection antibody respectively, due to the highest sensitivity for PCT detection with no cross reaction to calcitonin gene-related peptides. The proposed CLEIA based on mAb pair of 2D3/8F6-AP was characterized for a working range from 0.03 to 100 µg/L. An excellent correlation was observed between our proposed assay and the VIDAS BRAHMS PCT assay (r: 0.9825). CONCLUSION: Our newly developed mAbs and CLEIA can serve as important diagnostic tools for measurement of human PCT in serum.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Inmunoensayo , Magnetismo , Polipéptido alfa Relacionado con Calcitonina/sangre , Biomarcadores/sangre , Ensayo de Inmunoadsorción Enzimática , Humanos , Límite de Detección , Mediciones Luminiscentes , Valor Predictivo de las Pruebas , Polipéptido alfa Relacionado con Calcitonina/inmunología , Reproducibilidad de los ResultadosRESUMEN
In this work, a facile and catalyst-free method is developed to synthesize CdSe quantum wires (QWs) in organic solvent. Analysis on the intermediate products reveals that assembly of the initially-formed magic-sized CdSe clusters via oriented attachment plays a crucial role for generation of the well-defined QWs. Due to its simplicity, this method can be extended to production of many types of semiconductor QWs.