Transcription factor StWRKY1 is involved in monoterpene biosynthesis induced by light intensity in Schizonepeta tenuifolia Briq.

Menthone-type monoterpenes are the main active ingredients of Schizonepeta tenuifolia Briq. Previous studies have indicated that light intensity influences the synthesis of menthone-type monoterpenes in S. tenuifolia, but the mechanism remains unclear. WRKY transcription factors play a crucial role in plant metabolism, yet their regulatory mechanisms in S. tenuifolia are not well understood. In this study, transcriptome data of S. tenuifolia leaves under different light intensities were analyzed, identifying 57 candidate transcription factors that influence monoterpene synthesis. Among these, 7 members of the StWRKY gene family were identified and mapped onto chromosomes using bioinformatics methods. The physicochemical properties of the proteins encoded by these StWRKY genes, their gene structures, and cis-acting elements were also studied. Comparative genomics and phylogenetic analyses revealed that Sch000013479 is closely related to AaWRKY1, AtWRKY41, and AtWRKY53, and it was designated as StWRKY1. Upon silencing and overexpressing the StWRKY1 transcription factor in S. tenuifolia leaves, changes in the expression of key genes in the menthone-type monoterpene synthesis pathway were observed. Specifically, when StWRKY1 was effectively silenced, the content of (-)-pulegone significantly decreased. These results enhance our understanding of the impact of StWRKYs on monoterpene synthesis in S. tenuifolia and lay the groundwork for further exploration of the regulatory mechanisms involved in the biosynthesis of menthone-type monoterpenes.

[Accumulation and biosynthesis mechanism of volatile oils in glandular scale of Agastache rugosa].

The volatile oils are the effective components of Agastache rugosa, which are stored in the glandular scale. The leaves of pulegone-type A. rugosa were used as materials to observe the leaf morphology of A. rugosa at different growth stages, and the components of volatile oils in gland scales were detected by GC-MS. At the same time, qRT-PCR was used to determine the relative expression of key enzyme genes in the biosynthesis pathway of monoterpenes in volatile oils. The results showed that the density of A. rugosa glandular scale decreased first and then tended to be stable. With the growth of leaves, the relative content of pulegone decreased from 79.26% to 3.94%(89.97%-41.44%), while that of isomenthone increased from 2.43% to 77.87%(0.74%-51.01%), and the changes of other components were relatively insignificant. The correlation analysis between the relative content of monoterpenes and the relative expression levels of their key enzyme genes showed that there was a significant correlation between the relative content of menthone and isomenthone and the relative expression levels of pulegone reductase(PR)(r>0.6, P<0.01). To sum up, this study revealed the accumulation rules of the main components of the contents of the glandular scale of A. rugosa and the expression rules of the key enzyme genes for biosynthesis, which provided a scientific basis and data support for determining the appropriate harvesting period and quality control of the medicinal herbs. This study also initially revealed the biosynthesis mechanism of the monoterpenes mainly composed of pulegone and isomenthone in A. rugosa, laying a foundation for further research on the molecular mechanism of synthesis and accumulation of monoterpenes in A. rugosa.