Perioperative changes of serum orphanin in diabetic patients and its relationship with sympathetic nervous system.

The occurrence of cardiovascular events in diabetic patients during the perioperative period is related to the activation of sympathetic nerves. Basic research shows that serum nociceptin/orphanin FQ (N/OFQ) levels in diabetic neuropathy rats increased, and N/OFQ reduces the release of norepinephrine (NE). We hypothesize that N/OFQ will affect the sympathetic nervous system during perioperative myocardium of diabetic patients. 66 patients with unilateral knee arthroplasty were divided into diabetes group (D group) and non-diabetes group (N group). Measured blood glucose, serum NE, N/OFQ concentrations at the 30 min before anesthesia (T0), 1 h after surgery (T1), 24 h after surgery (T2) and the cardiac troponinI (cTnI) concentration at T0 and T2. Compared with N group, the concentration of blood glucose, N/OFQ and cTnI in D group was higher and the NE was lower at T0 (P < 0.05). At T1, the blood glucose, N/OFQ, NE concentrations of D group increased, only the blood glucose increased in N group (P < 0.05). Serum N/OFQ of D group from T0 to T1 was correlated with the change trend of blood glucose, NE concentration from T0 to T1 and cTnI from T0 to T2(r = 0.386, P = 0.027; r = 0.350, P = 0.046; r = 0.363, P = 0.038). The outcomes demonstrated that the preoperative serum N/OFQ concentration in diabetic patients was increased, and the increase in N/OFQ concentration during the operation was related to the increase in NE and cTnI concentrations, perioperative N/OFQ may mediate myocardial injury through sympathetic nervous system.

Expression and significance of MMP-9 and MDM2 in the oncogenesis of lung cancer in rats

OBJECTIVE@#To observe the expression of matrix metalloproteinase-9 (MMP-9) and mouse double minute 2 homolog (MDM2) in the oncogenesis of lung cancer in rats and to explore their clinical value.@*METHODS@#A total of 140 rats were selected, of which 20 were selected randomly as the control group; and the remaining 120 as the observation group. The observation group was injected with benzopyrene to establish diseases model such as tissue proliferation, abnormal proliferation and lung cancer. Detected the MMP-9 levels of lung tissue by enzyme-linked assay, detected the MDM2 levels of lung tissue by immunochemistry assay.@*RESULTS@#The MMP-9 and MDM2 expression of the lung cancer group and the abnormal proliferation group were significantly higher than that in the tissue proliferation group and the control group, the difference was significant (P0.05). The MDM2 and MMP-9 expression were increased in turn in the small cell carcinoma, squamous cell carcinoma and adenocarcinoma, the difference was statistically significant (P0.05), and there is no significant difference of the MMP-9 and MDM2 expressions between stage I and stage II(P>0.05).@*CONCLUSIONS@#The expression of MMP-9 and MDM2 in lung tissue was associated with lung disease and lung cancer, both of them may be involved in the development and metastasis of lung cancer. Combined detection can be used as therapy and prognostic indicators for lung cancer.