Interfacial crystallized oleogel emulsion with improved freeze-thaw stability and tribological properties: Influence of cooling rate.

In this study, the influence of cooling rate on the freeze-thaw stability, rheological and tribological properties of interfacial crystalized oleogel emulsion was investigated. Results showed that slower cooling rate could promote formation of larger crystals and stronger network in oleogels. Additionally, oleogel emulsions showed higher freeze-thaw stability than those stabilized solely by emulsifiers. The slower cooling rate resulted in larger crystals adsorbed at the droplet surface. This led to greater steric hindrance that prevented the migration of oil droplets with higher resistance to disruption by ice crystals. The rheological and tribological measurements suggested that with appropriate amount of crystals, the tribological properties were better maintained for emulsions prepared at slow cooling rate after freeze-thaw treatment. This strategy greatly enriched oleogel emulsion formulations and provided important clues for potential applications in food products involved with freeze-thaw treatment.

Construction of interfacial crystallized oleogel emulsion with improved thermal stability.

In this study, we reported a facile strategy to produce an interfacial crystallized oleogel emulsion for improved thermal stability. The interfacial crystallization of ceramide (non-interfacial active oleogelator) was achieved by addition of a surface active compound, which was demonstrated by interfacial rheology tests and polarized light microscopy. For successfully prepared interfacial crystallized emulsions, smaller particle size was observed when the gelator concentration was lower. However, better thermal stability was achieved when oleogelator concentration was higher than 1 wt%. Results from differential scanning calorimetry, X-ray diffraction and Fourier transform infrared spectroscopy suggested that the interfacial adsorption of ceramide was due to its co-crystallization with the emulsifier driven by hydrogen bonds formed by multiple sites. It provided appropriate crystallinity and steric repulsion for oleogel emulsions against oil droplet coalescence during heating process. This strategy greatly enriches oleogel emulsion formulations and their potential applications in food products involved with thermal treatment.

Hydraulic characteristics in channel confluence affect the nitrogen dynamics through altering interactions among multi-trophic microbiota.

Identifying the distribution of multi-trophic microbiota under the complicated hydrodynamic characteristics of channel confluences and evaluating the microbial contributions to biogeochemical processes are vital for river regulation and ecological function protection. However, relevant studies mainly focus on bacterial community distribution in confluence, neglecting the essential role of multi-trophic microbiota in the aquatic ecosystems and biogeochemical processes. To address this knowledge gap, this study investigated the distribution of multi-trophic microbiota and the underlying assembly process under the hydraulic characteristics in the confluence and described the direct and indirect effects of multi-trophic microbiota on the nitrogen dynamics. Results revealed that, in a river confluence, eukaryotic communities were governed by deterministic processes (52.4%) and bacterial communities were determined by stochastic processes (74.3%). The response of higher trophic levels to environmental factors was intensively higher than that of lower trophic microbiota, resulting in higher trophic microbiota were significantly different between regions with varied environmental conditions (P < 0.05). Flow velocity was the driving force controlling the assembly and composition of multi-trophic microbiota and interactions among multi-trophic levels, and further made a significant difference to nitrogen dynamics. In regions with lower flow velocity, interactions among multi-trophic levels were more complex. There were intense nitrate and nitrite reduction and anammox reactions via direct impacts of protozoan and metazoan and the top-down control (protozoan and metazoan prey on heterotrophic bacteria) among multi-trophic microbiota. Results and findings reveal the ecological effect on river nitrogen removal in a river confluence under complex hydraulic conditions and provide useful information for river management.

The distribution and ecological risks of antibiotics in the sediments from a diverging area of the bifurcated river: Effects of hydrological properties.

The hydrodynamics in the diverging area become complicated because of the basin hydrological conditions, making the distribution of antibiotics largely uncertain and thus bringing uncertain ecological risks of antibiotics. Through field sampling, experiments and numerical simulations, the distribution of antibiotics, its responses to hydrological conditions were studied. Antibiotics in the bifurcated river sediments was mainly distributed in the branch mouth. The hydrodynamic regions were affected by the hydrological frequency. Notably, the center of the low-velocity area moved upstream and gradually expands to the entire tributary as the hydrological frequency shifted from high to low. ENRO (enrofloxacin) and OFC (ofloxacin) were the key hazardous antibiotics affecting the ecological health in the diverging area, and their concentrations are mainly affected by sediment particle size (D < 0.15 mm) and oxygen content. The ecological risk of antibiotics in the diverging area were gradually decreased with the increase of the distance from the central area. The water physical and chemical properties, altered by the river basin hydrological conditions, play an important role in influencing the distribution of antibiotic concentrations, and ultimately posing great threat to aquatic ecosystem. The research provides a scientific basis for antibiotic risk control in the diverging area under different hydrological conditions.

Hydrodynamic zones and the influence of microorganisms on nitrogen transformation in the diverging area of branched rivers.

Diverging area is widespread in river networks, and understanding its biogeochemical process characteristics is of great significance to river ecological restoration and environmental quality improvement. Microbial communities affected by hydrodynamics play an important role in biogeochemical processes, but their relationship in diverging area is little known. Here, the composition of microbial community and its feedback to hydrodynamics and nitrogen conversion in the diverging area of river networks were first studied by coupling ecological theory, biogeochemical theory, microbial DNA sequencing and mathematical model of water environment. The results showed that there were five hydrodynamic zones with significant velocity differences in the diverging area, namely low velocity zone, maximum velocity zone, stagnant zone, separation zone, and deflection zone. According to the flow velocity grouping, there were significant differences in the microbial diversity and abundance among low velocity group, maximum velocity group and stagnant group had significant differences (p < 0.05, stress = 0.1207). In the low velocity group, Firmicutes was the dominant phylum which had a highest abundance and may promot the conversion of organic nitrogen into ammonia nitrogen. In the maximum velocity group, Bdellovibrionota was the dominant phylum which had a highest abundance and may promot the conversion of nitrate and nitric oxide to nitrogen. In the stagnant zone, Methylomirabilota was the dominant phylum which had a highest abundance and may promot the conversion of nitrogen into nitrate and ammonium. In addition, dissolved oxygen was the most sensitive environmental factor for shaping microorganisms and nitrogen conversion in the diverging area of the river networks by canonical correlation analysis. The denitrifying bacteria Rhodocyclaceae, was shown to negatively correlated with the flow velocity. This research improves the scientific basis for the study of the ecosystem in river networks, which will guide the construction of river ecological projects.