Uptake and elimination of 4-nonylphenol by the clam Tapes philippinarum.

The present study is the first dealing with the accumulation and elimination of 4-nonylphenol (NP) by the Manila clam, Tapes philippinarum. Specimens of T. philippinarum were exposed to NP-spiked seawater, and the NP contents in whole soft tissue, gills, digestive gland, and haemolymph were measured. Actual NP concentrations in seawater and microalgae (Isochrysis galbana) used for feeding were also determined, and the algal bioconcentration factor (BCF) value (640 ml/g) was calculated. Volatilisation was the main cause of dissipation of NP from experimental tanks, attaining up to 78% of the NP added. NP accumulated by algae used for feeding was negligible with respect to the total added NP, and we concluded that in our experiments, clams accumulated NP mainly from water and not food intake. Accumulation followed a two-compartment, first-order rate coefficient model, with an uptake rate coefficient of 13.8 +/- 0.6 mL g(-1)h(-1)(fresh weight [fw]) and an elimination coefficient of 0.0070 +/- 0.0005 h(-1). Ninety percent of the steady state was reached after 14 days of exposure, and the BCF value at the steady state was 1958 +/- 158 mL g(-1) fw (1.8 +/- 0.2 x 10(5) based on lipid weight). Slightly less than 50% of NP bioaccumulated through water was allocated into the gills, whereas the rest was found in the digestive gland. In the first 8 hours, clams eliminated 51% of the NP accumulated, and only 2% of the NP accumulated was detected in the clams at the end of the elimination phase (day 14). Two-compartment first-order decay model described the elimination of the accumulated NP by considering the clam as two compartments each with a different elimination rate. The sudden elimination of NP in the initial hours can be attributed to the elimination of NP accumulated into the gills and readily available for excretion (k ( e ) = 0.30 +/- 0.07 h(-1)). The slower step of the elimination process should be the mobilisation of NP accumulated in internal organs, which must be carried into the haemolymph for excretion (k(e) = 0.0091 +/- 0.0002 h(-1)). Because T. philippinarum has been demonstrated to accumulate NP dissolved in water, high NP levels can be hypothesised in clams from highly contaminated environments. This research was performed according to all national and international guidelines for animal welfare.

[Measurement of venous pressure by Doppler sonography: usefulness and limitations in saphenectomy]. / Misurazione della pressione venosa con la Doppler sonografia: utilità e limiti nell'intervento di safenectomia.

After a short mention on the many last methods for venous pressure measurement, the Authors presents a relatively new method of this measurement with the Doppler ultrasonography. They point out its utility with regard to safenectomy by a study on 42 patients whose, at the present, only 10 preliminary results are available. This results allow to verify the opportunity of to value preoperatory and precisely the pressure drop obtainable with the safenectomy.