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1.
Braz J Med Biol Res ; 51(9): e7602, 2018 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-30020320

RESUMEN

The NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is the most frequently studied in the central nervous system and has been linked to neuropathic pain. In this study, a post-translational mechanism of microRNA (miR)-186 via regulating the expression of NLRP3 in the complete Freund's adjuvant (CFA)-treated mice was investigated. The injection of CFA was used to induce trigeminal neuropathic pain in mice. miRs microarray chip assay was performed in trigeminal ganglions (TGs). CFA treatment significantly increased the mRNA expression of NLRP3, interleukin (IL)-1ß, and IL-18 in TGs compared to the control group. Moreover, 26 miRs were differentially expressed in TGs from trigeminal neuropathic pain mice, and the expression of miR-186 showed the lowest level of all the miRs. Further examination revealed that NLRP3 was a candidate target gene of miR-186. We delivered miR-186 mimics to CFA-treated mice. The head withdrawal thresholds of the CFA-treated mice were significantly increased by miR-186 mimics injection compared with CFA single treatment. The mRNA and protein expression of NLRP3, IL-1ß, and IL-18 in TGs from trigeminal neuropathic pain mice were significantly inhibited by miR-186 mimics treatment compared to the CFA group. miR-186 was able to suppress the neuropathic pain via regulating the NLRP3 inflammasome signaling.


Asunto(s)
Inflamasomas/fisiología , MicroARNs/farmacología , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Neuralgia del Trigémino/tratamiento farmacológico , Neuralgia del Trigémino/genética , Animales , Western Blotting , Modelos Animales de Enfermedad , Ensayo de Inmunoadsorción Enzimática , Adyuvante de Freund , Estudios de Asociación Genética , Inflamasomas/análisis , Interleucina-18/análisis , Interleucina-18/metabolismo , Interleucina-1beta/análisis , Interleucina-1beta/metabolismo , Luciferasas , Masculino , Ratones Endogámicos C57BL , Análisis por Micromatrices , Proteína con Dominio Pirina 3 de la Familia NLR/análisis , Distribución Aleatoria , Reacción en Cadena en Tiempo Real de la Polimerasa , Valores de Referencia , Factores de Tiempo , Neuralgia del Trigémino/inducido químicamente , Neuralgia del Trigémino/metabolismo
2.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;51(9): e7602, 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-951757

RESUMEN

The NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is the most frequently studied in the central nervous system and has been linked to neuropathic pain. In this study, a post-translational mechanism of microRNA (miR)-186 via regulating the expression of NLRP3 in the complete Freund's adjuvant (CFA)-treated mice was investigated. The injection of CFA was used to induce trigeminal neuropathic pain in mice. miRs microarray chip assay was performed in trigeminal ganglions (TGs). CFA treatment significantly increased the mRNA expression of NLRP3, interleukin (IL)-1β, and IL-18 in TGs compared to the control group. Moreover, 26 miRs were differentially expressed in TGs from trigeminal neuropathic pain mice, and the expression of miR-186 showed the lowest level of all the miRs. Further examination revealed that NLRP3 was a candidate target gene of miR-186. We delivered miR-186 mimics to CFA-treated mice. The head withdrawal thresholds of the CFA-treated mice were significantly increased by miR-186 mimics injection compared with CFA single treatment. The mRNA and protein expression of NLRP3, IL-1β, and IL-18 in TGs from trigeminal neuropathic pain mice were significantly inhibited by miR-186 mimics treatment compared to the CFA group. miR-186 was able to suppress the neuropathic pain via regulating the NLRP3 inflammasome signaling.


Asunto(s)
Animales , Masculino , Neuralgia del Trigémino/tratamiento farmacológico , MicroARNs/farmacología , Inflamasomas/fisiología , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Valores de Referencia , Factores de Tiempo , Ensayo de Inmunoadsorción Enzimática , Distribución Aleatoria , Adyuvante de Freund , Western Blotting , Interleucina-18/análisis , Interleucina-18/metabolismo , Análisis por Micromatrices , Modelos Animales de Enfermedad , Interleucina-1beta/análisis , Interleucina-1beta/metabolismo , Estudios de Asociación Genética , Inflamasomas/análisis , Proteína con Dominio Pirina 3 de la Familia NLR/análisis , Luciferasas , Ratones Endogámicos C57BL
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