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1.
J Org Chem ; 89(7): 4503-4511, 2024 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-38502929

RESUMEN

Tetrahydroisoquinoline (THIQ) derivatives stand out as a promising class of compounds due to their diverse range of biological activities, making them particularly valuable in drug discovery. To enhance their structural diversity, an Rh-catalyzed denitrogenative annulation method has been introduced for synthesizing these derivatives. An intriguing aspect of this method is the ability of the Brønsted acid to prevent further annulation while facilitating the production of the desired THIQ derivatives, achieving impressive yields of up to 86%. This synthetic approach was subsequently leveraged to create an analogue of cyclocelabenzine, a compound showing potential as an anti-inflammatory agent.

2.
Bioconjug Chem ; 2023 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-36976658

RESUMEN

Breast cancer (BC) is one of the most common malignancies in the world with aberrantly expressed glycans. The different types and stages still limit a comprehensive method in the prediagnosis of BC patients. In this research, a synthetic boronic acid-disulfide (BASS) probe has been developed for the two steps of O → S → N acyl transfer in glycoprotein recognition and labeling. The specificity and sensitivity of this method have been carefully studied in the case of immunoglobulin G, and the labeling efficiency was determined up to 60%. The BASS-functionalized slide is a powerful platform for monitoring the alteration of glycan patterns in human sera. Compared to the samples from healthy individuals, sera of BC patients gave specific patterns to eight lectins binding. The BASS-directed glycoprotein strategy promises a rapid sensing platform for a high-throughput screening of clinical BC samples and could be easily applied to other cancer prediagnoses.

3.
Biosens Bioelectron ; 200: 113885, 2022 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-34954569

RESUMEN

Several neurodegenerative diseases are ascribed to disorders caused by the secretion of Cu ions. However, a majority of the current techniques for copper ion detection are restricted to in vivo monitoring and nonspecific interactions. Their methods are limited to the systematic analysis of Cu ions in living organisms. Thus, a synthetic molecular fluorophore, 5-amino 2,3-dihydroquinolinimine (NDQI), has been developed and successfully utilized in in vivo monitoring of the distribution of Cu(II) in zebrafish larvae. The reversible formation of the NDQI-Cu complex allows its use with high metal concentrations and in oxidative stress conditions. The NDQI-directed strategy developed here can quantitatively differentiate cells with different Cu(II) concentrations. Remarkably, dynamic distribution of Cu(II) in the intestine and liver can be observed.


Asunto(s)
Técnicas Biosensibles , Pez Cebra , Animales , Cobre , Colorantes Fluorescentes , Larva
4.
Clin Chim Acta ; 512: 106-111, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31790698

RESUMEN

BACKGROUND: Progesterone is one of the female steroid hormones and plays an important role in the menstrual cycle and during pregnancy. It is especially important in preparing the uterus for the implantation of the blastocyst and maintaining pregnancy. The concentration in human serum is measured to determine the ovarian function retroactively and the cause of abortion in early pregnancy. METHODS: A quantification assay based on isotope dilution mass spectrometry to determine the concentration of progesterone in human serum is reported. Incorporated with 13C3-progesterone, serum samples were subjected to progesterone extraction and clean-up by C4 solid-phase-extraction columns and hexane-based liquid/liquid extraction, respectively. The cleaned-up serum samples were then subjected to MALDI-TOF mass spectrometry for the quantification of progesterone. RESULTS: Progesterone and the internal standard, 13C3-progesterone, were measured in the selected reaction monitoring mode for the transitions m/z 315.4 to 108.9 and m/z 318.4 to 111.9, respectively. We calculated the peak area ratio of progesterone to 13C3-progesterone. The progesterone concentration in human serum was calculated by substituting the peak area ratio into an isotope dilution calibration curve, and then compared with the radioimmunoassay. CONCLUSIONS: In the study, the concentrations of serum progesterone were measured, and the recovered progesterone concentration determined by the assay showed good robustness and consistency in comparison to the conventional radioimmunologic assay. We concluded that the 13C3-progesterone-based quantification assay is a robust method for the measurement of serum progesterone.


Asunto(s)
Isótopos , Progesterona , Femenino , Humanos , Técnicas de Dilución del Indicador , Radioinmunoensayo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
5.
Chem Commun (Camb) ; 56(76): 11307-11310, 2020 Sep 28.
Artículo en Inglés | MEDLINE | ID: mdl-32840276

RESUMEN

This study monitors the dynamic progress of a newly developed background-free, target responsive strategy; 2,3-dihydroquinolin-4-imine (DQI) that can instantly respond to environmental changes with fluorescence enhancement, revealing a comprehensive platform for in vivo fluorescence bioimaging of mebrane-bound carbonic anhydrase II in HeLa cells and its expression during the growth of larval zebrafish.


Asunto(s)
Anhidrasa Carbónica II/biosíntesis , Colorantes Fluorescentes/análisis , Colorantes Fluorescentes/química , Quinolinas/química , Pez Cebra/crecimiento & desarrollo , Animales , Anhidrasa Carbónica II/antagonistas & inhibidores , Anhidrasa Carbónica II/metabolismo , Inhibidores de Anhidrasa Carbónica/farmacología , Teoría Funcional de la Densidad , Colorantes Fluorescentes/síntesis química , Células HeLa , Humanos , Larva/enzimología , Larva/crecimiento & desarrollo , Estructura Molecular , Imagen Óptica , Quinolinas/síntesis química , Sulfonamidas/farmacología
6.
ACS Appl Bio Mater ; 3(10): 6756-6767, 2020 Oct 19.
Artículo en Inglés | MEDLINE | ID: mdl-35019340

RESUMEN

Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation control over antibody immobilization. Here, we report a mild electrophilic phenylsulfonate (tosylate) ester-containing boronic acid affinity ligand for attaching antibodies to glass slides. A high level of antibody coupling located near the Fc region of the boronated antibody complex could be achieved by the proximal nucleophilic amino acid driven substitution reaction at the phenylsulfonate center. This enabled the full-length antibodies to be permanently tethered onto surfaces in an oriented manner. The advantages of this strategy were demonstrated through the individual and multiplex detection of protein and serum biomarkers. This strategy not only confers stability to the immobilized antibodies but also presents a different direction for the irreversible attachment of antibodies to solid supports in an orientation-controlled way.

7.
J Food Drug Anal ; 27(2): 551-564, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30987727

RESUMEN

Alzheimer's disease (AD) is the most common cause of dementia in late life. It is difficult to precisely diagnose AD at early stages, making biomarker search essential for further developments. The objective of this study was to identify protein biomarkers associated with aluminum ions toxicity (AD-like toxicity) in a human neuroblastoma cell model, SH-SY5Y and assess potential prevention by NAP (NAPVSIPQ). Complete proteomic techniques were implemented. Four proteins were identified as up-regulated with aluminum ion treatment, CBP80/20-dependent translation initiation factor (CTIF), Early endosome antigen 1 (EEA1), Leucine-rich repeat neuronal protein 4 (LRRN4) and Phosphatidylinositol 3-kinase regulatory subunit beta (PI3KR2). Of these four proteins, EEA1 and PI3KR2 were down-regulated after NAP-induced neuroprotective activity in neuroblastoma cells. Thus, aluminum ions may increase the risk for neurotoxicity in AD, and the use of NAP is suggested as a treatment to provide additional protection against the effects of aluminum ions, via EEA1 and PI3KR2, associated with sorting and processing of the AD amyloid precursor protein (APP) through the endosomal system.


Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Fármacos Neuroprotectores/farmacología , Fragmentos de Péptidos/farmacología , Bibliotecas de Moléculas Pequeñas/farmacología , Aluminio/toxicidad , Enfermedad de Alzheimer/inducido químicamente , Enfermedad de Alzheimer/patología , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Humanos , Iones/toxicidad , Fármacos Neuroprotectores/química , Neurotoxinas/toxicidad , Oxidación-Reducción , Fragmentos de Péptidos/química , Bibliotecas de Moléculas Pequeñas/química , Relación Estructura-Actividad , Células Tumorales Cultivadas
8.
ACS Appl Bio Mater ; 2(3): 1286-1297, 2019 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-35021376

RESUMEN

Protein glycosylation is one of the most complicated but significant post-translational modifications. Minor alterations in glycan structure can considerably affect the biology of a cell. Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis. In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins. To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data. The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation. Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique. Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.

9.
Adv Sci (Weinh) ; 5(9): 1800638, 2018 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30250802

RESUMEN

Hydrogels with tunable viscoelasticity hold promise as materials that can recapitulate many dynamic mechanical properties found in native tissues. Here, covalent adaptable boronate bonds are exploited to prepare hydrogels that exhibit fast relaxation, with relaxation time constants on the order of seconds or less, but are stable for long-term cell culture and are cytocompatible for 3D cell encapsulation. Using human mesenchymal stem cells (hMSC) as a model, the fast relaxation matrix mechanics are found to promote cell-matrix interactions, leading to spreading and an increase in nuclear volume, and induce yes-associated protein/PDZ binding domain nuclear localization at longer times. All of these effects are exclusively based on the hMSCs' ability to physically remodel their surrounding microenvironment. Given the increasingly recognized importance of viscoelasticity in controlling cell function and fate, it is expected that the synthetic strategies and material platform presented should provide a useful system to study mechanotransduction on and within viscoelastic environments and explore many questions related to matrix biology.

10.
Biomacromolecules ; 19(7): 3086-3095, 2018 07 09.
Artículo en Inglés | MEDLINE | ID: mdl-29890078

RESUMEN

Antibody and related antibody drugs for the treatment of malignancies have led to progress in targeted cancer therapy. Preparation of diverse antibody conjugates is critical for preclinical and clinical applications. However, precise control in tagging molecules at specific locations on antibodies is essential to preserve their native function. In this study, a synthetic boronic acid (BA)-tosyl initiator was used to trigger a glycan-directed modification of IgGs, and the obtained IgG macroinitiators allowed a growth of the poly N-isopropylacrylamide (PNIPAAm) chains specifically at Fc-domains. Therefore, the PNIPAAm chains are located away from the critical antigen-binding domains (Fab), which could reasonably prevent the loss of biological activity after the attachment of polymer chains. According to the proposed strategy, a site-selectively modified anticoncanavalin A (Con A) antibody-PNIPAAm conjugate showed 6-times higher efficiency in the binding of targeted Con A antigen to a randomly conjugated anti-Con A antibody-PNIPAAm conjugate. In this study, we developed the first chemical strategy for the site-specific preparation of IgG-polymer conjugates with conserved biological activity as well as intact glycan structures.


Asunto(s)
Inmunoglobulina G/química , Polisacáridos/química , Resinas Acrílicas/química , Concanavalina A/inmunología , Fragmentos Fab de Inmunoglobulinas/química , Inmunoglobulina G/inmunología , Polimerizacion
11.
Oncotarget ; 9(1): 442-452, 2018 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-29416626

RESUMEN

Glycine N-methyltransferase is a tumor suppressor gene for hepatocellular carcinoma, which can activate DNA methylation by inducing the S-adenosylmethionine to S-adenosylhomocystine. Previous studies have indicated that the expression of Glycine N-methyltransferase is inhibited in hepatocellular carcinoma. To confirm and identify missing proteins, the pathologic analysis of the tumor-bearing mice will provide critical histologic information. Such a mouse model is applied as a screening tool for hepatocellular carcinoma as well as a strategy for missing protein discovery. In this study we designed an analysis platform using the human proteome atlas to compare the possible missing proteins to human whole chromosomes. This will integrate the information from animal studies to establish an optimal technique in the missing protein biomarker discovery.

12.
Chemistry ; 24(5): 1112-1120, 2018 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-29044757

RESUMEN

In this study, a series of variously substituted 2,3-dihydroquinolin-4-imines (DQIs) were synthesized from N-substituted propargylanilines by copper(I)-catalyzed annulation. The approach adopted in this study under mild, effective conditions exhibited broad substrate tolerance, particularly for functional groups substituted on anilines. Most of the DQI derivatives synthesized under optimal conditions were obtained in good isolated yields of 63-88 %. 2,3-Dihydroquinolinimine thus obtained was easily converted to important structures like 2,3-dihydroquinolone and tetrahydrobenzodiazepin-5-one, confirming the importance of this strategy in constructing various heterocycles. Surprisingly, 2,3-dihydroquinolinimines thus obtained exhibited bright fluorescence with quantum yields up to 66 %. The density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations were performed for understanding the excited-state nature of DQI system. Accordingly, a tailored DQI derivative bearing methoxy group at C-6 position and acetoxy group at C-7 position was designed and synthesized to give emission at 559 nm with redshift compared to the 7-methoxy substituted DQI. A detailed study of DQI structures with their photophysical properties was performed with five control molecules and consequently demonstrated the uniqueness of the chemical structures of DQIs.

13.
Chem Asian J ; 12(12): 1326-1337, 2017 Jun 19.
Artículo en Inglés | MEDLINE | ID: mdl-28395122

RESUMEN

A copper(I)-mediated denitrogenative reaction has been successfully developed for the preparation of cyclic tetrapeptides. The key reactive intermediate, ketenimine, triggers intramolecular cyclization through attack of the terminal amine group to generate an internal ß-amino acid with an amidine linkage. The chemistry developed herein provides a new synthetic route for the preparation of cyclic α3 ß-tetrapeptide analogues that contain important biological properties and results in rich structural information being obtained for conformational studies. With the success of this copper(I)-catalyzed macrocyclization, two histone deacetylase inhibitor analogues consisting of the cyclic α3 ß-tetrapeptide framework have been successfully synthesized.


Asunto(s)
Cobre/química , Inhibidores de Histona Desacetilasas/síntesis química , Oligopéptidos/síntesis química , Péptidos Cíclicos/síntesis química , Ciclización , Inhibidores de Histona Desacetilasas/química , Estructura Molecular , Oligopéptidos/química , Péptidos Cíclicos/química
14.
Biomed Res Int ; 2017: 4051763, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28127555

RESUMEN

Micelles, with the structure of amphiphilic molecules including a hydrophilic head and a hydrophobic tail, are recently developed as nanocarriers for the delivery of drugs with poor solubility. In addition, micelles have shown many advantages, such as enhanced permeation and retention (EPR) effects, prolonged circulation times, and increased endocytosis through surface modification. In this study, we measured the critical micelle concentrations, diameters, stability, and cytotoxicity and the cell uptake of micelles against hepatic cells with two kinds of hydrophilic materials: PEG-PCL and HA-g-PCL. We used 131I as a radioactive tracer to evaluate the stability, drug delivery, and cell uptake activity of the micelles. The results showed that HA-g-PCL micelles exhibited higher drug encapsulation efficiency and stability in aqueous solutions. In addition, the 131I-lipiodol loaded HA-g-PCL micelles had better affinity and higher cytotoxicity compared to HepG2 cells.


Asunto(s)
Sistemas de Liberación de Medicamentos , Aceite Etiodizado/administración & dosificación , Radioisótopos de Yodo/administración & dosificación , Radiofármacos/administración & dosificación , Línea Celular , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Medios de Contraste/administración & dosificación , Medios de Contraste/farmacocinética , Medios de Contraste/toxicidad , Portadores de Fármacos/química , Estabilidad de Medicamentos , Aceite Etiodizado/farmacocinética , Aceite Etiodizado/toxicidad , Células Hep G2 , Hepatocitos/efectos de los fármacos , Hepatocitos/metabolismo , Hepatocitos/efectos de la radiación , Humanos , Ácido Hialurónico/análogos & derivados , Interacciones Hidrofóbicas e Hidrofílicas , Radioisótopos de Yodo/farmacocinética , Radioisótopos de Yodo/toxicidad , Micelas , Tamaño de la Partícula , Poliésteres , Radiofármacos/farmacocinética , Radiofármacos/toxicidad , Solubilidad
15.
Chem Asian J ; 11(5): 757-65, 2016 Mar 04.
Artículo en Inglés | MEDLINE | ID: mdl-26789740

RESUMEN

A rapid growth in synthetic methods for the preparation of diverse organic molecules using N-sulfonyl-1,2,3-triazoles is of great interest in organic synthesis. Transition metals are generally used to activate the α-imino diazo intermediates. Metal-free methods have not been studied in detail, but can be a good complement to transition metal catalysis in the mild reaction conditions. We herein report a novel method for the preparation of 2,3-dihydroquinolin-4-imine and chroman-4-imine analogs from their corresponding N-sulfonyl-1,2,3-triazoles in the absence of metal catalysts. To achieve intramolecular annulation, the introduction of an electron-donating group is required at the meta position of N-sulfonyl-1,2,3-triazole methyl anilines. The inclusion of tailored substituents on the aniline moieties and nitrogen atoms enhances the nucleophilicity of the phenyl π-electrons, thus allowing them to undergo a Friedel-Crafts-type reaction with the highly electrophilic ketenimines. This metal-free method was carefully optimized to generate a variety of dihydroquinolin-4-imines and chroman-4-imines in moderate-to-good yields.


Asunto(s)
Cromanos/síntesis química , Iminas/síntesis química , Quinolinas/síntesis química , Triazoles/química , Cromanos/química , Cristalografía por Rayos X , Iminas/química , Modelos Moleculares , Quinolinas/química , Compuestos de Azufre/química , Temperatura
16.
Biomed Res Int ; 2016: 1917394, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-28053978

RESUMEN

The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid (HA) biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media (PC12 CM) in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS/MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.


Asunto(s)
Adhesión Celular/efectos de los fármacos , Ácido Hialurónico/administración & dosificación , Neuroblastoma/metabolismo , Ingeniería de Tejidos , Animales , Proliferación Celular/efectos de los fármacos , Microambiente Celular/efectos de los fármacos , Chaperonina 60/biosíntesis , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Humanos , Proteínas Mitocondriales/biosíntesis , Chaperonas Moleculares/biosíntesis , Regeneración Nerviosa/genética , Neuroblastoma/tratamiento farmacológico , Neuroblastoma/patología , Neuronas/metabolismo , Neuronas/fisiología , Proteínas de Complejo Poro Nuclear/biosíntesis , Células PC12 , Ratas
17.
Int J Mol Sci ; 16(1): 1657-76, 2015 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-25588218

RESUMEN

Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 µg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.


Asunto(s)
Quitosano/metabolismo , Portadores de Fármacos/metabolismo , Fibroínas/metabolismo , Nanopartículas/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Ubiquitina/metabolismo , Materiales Biocompatibles/metabolismo , Línea Celular , Células Hep G2 , Humanos , Proteómica , Transducción de Señal
18.
Org Lett ; 16(14): 3752-5, 2014 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-24968224

RESUMEN

An efficient Cu/Rh-catalyzed method is proposed for the synthesis of 3-indolylimines from N-propargylanilines through Rh(II)-catalyzed denitrogenative annulation of N-sulfonyl-1,2,3-triazoles. Further combined with hydrolysis or reduction, a one-pot method is developed to enable the direct incorporation of an imine, aldehyde, or amine group into an indole system from an alkyne. A variety of substituted 3-indolylimines, indole-3-carboxaldehydes, and 3-Indolylmethanamines are synthesized in good yields.

19.
Biomed Res Int ; 2014: 209469, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24818131

RESUMEN

The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.


Asunto(s)
Materiales Biocompatibles/farmacología , Fibroínas/farmacología , Proteínas/metabolismo , Proteómica , Animales , Bromodesoxiuridina/metabolismo , Adhesión Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Electrodos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Ontología de Genes , Receptores de Hialuranos/metabolismo , Inmunohistoquímica , Espectrometría de Masas , Microscopía de Fuerza Atómica , Nanotubos de Carbono/química , Tecnicas de Microbalanza del Cristal de Cuarzo , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie
20.
Biomed Res Int ; 2014: 789591, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24757677

RESUMEN

Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6 ± 11.2 nm, and the average zeta potential was +14.08 ± 0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation.


Asunto(s)
Proliferación Celular/efectos de los fármacos , Quitosano/farmacología , Neoplasias Hepáticas/metabolismo , Hígado/metabolismo , Nanopartículas , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/efectos de los fármacos , Serina-Treonina Quinasas TOR/metabolismo , Quitosano/química , Células Hep G2 , Humanos , Hígado/patología , Neoplasias Hepáticas/patología , Metástasis de la Neoplasia , Proteómica/métodos
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