RESUMEN
Fibroblast growth factor 9 (FGF9) is a member of the fibroblast growth factor family, which is widely expressed in the central nervous system (CNS). It has been reported that deletion of FGF9 leads to defects in cerebellum development, including Purkinje cell defect. However, it is not clear how FGF9 regulating cerebellar development remains to be determined. Our results showed that in addition to disrupt Bergmann fiber scaffold formation and granule neuron migration, deletion of neuronal FGF9 led to ataxia defects. It affected development and function of Purkinje cells, and also changed the action potential threshold and excitation frequency. Mechanistically, depletion of FGF9 significantly changed neurotransmitter contents in Purkinje cells and led to preferential increase in inflammation, even downregulation in ERK signaling. Together, the data demonstrate that neuronal FGF9 is required for the development and function of Purkinje cells in the cerebellum. Insufficient FGF9 during cerebellum development will cause ataxia defects.
RESUMEN
Sustainable development of rural has become an essential global plan. Habitat sustainability assessment of rural is a critical management tool to grasp the development status of rural in real-time and enable dynamic adjustment of policies. This paper combines the 2030 Sustainable Development Goals (SDGs) with the entropy weight method, TOPSIS, and grey correlation analysis to construct a multi-criteria decision-making (MCDM) evaluation model, which is finally used to assess the sustainability of the rural human settlement environment. Finally, this paper uses the rural of 11 prefecture-level cities in Zhejiang Province in 2021 as a case study for rural human settlement environment sustainability evaluation. The results show that the overall rural human settlement environment sustainability level in Zhejiang Province is better than in most regions in China. Hangzhou has the best rural human settlement environment sustainability, and Zhoushan has the worst. In addition, the production environment factor is the critical factor that constrains sustainability. The study results provide references and guidance to policymakers for sustainable development initiatives.
