A Multi-Criteria Framework for Identification of Gully Developmental Stages Based on UAV Data-A Case Study in Yuanmou County, Yunnan Province, SW China.

Gully erosion is a common form of soil erosion in dry-hot valleys, and it often brings serious land degradation. A multi-criteria method integrating the characteristics of the longitudinal profile (LP), the cross profile (CP) and the knickpoints of gullies was applied to identify the development stage of gullies in Yuanmou County, Yunnan Province, in southwestern China. Firstly, based on the high-resolution data sources produced by an unmanned aerial vehicle (UAV), 50 gullies were selected as the typical ones in Tutujiliangzi and Shadi village. The LPs were extracted, and their morphological indices, information entropy and fitting functions were calculated. The morphological characteristics of the CPs and the presence or absence of knickpoints were recorded. The results show that the period of the gullies in Tutujiliangzi and Shadi is dominated by the deep incision period and the equilibrium adjustment period, which means that most gullies are in the period of the severe erosion stage. Among the gullies, 13 LPs' morphological index is between 0.636 and 0.933, and the morphology of the LP presents an upward convex shape; the cross profiles are mainly V-shaped and U-shaped. Thirty-two LPs' morphological index is between 1.005~2.384, which presents a slightly concave shape; the cross profiles are mainly repeated U-shapes. The remaining five LPs have a morphological index of 0.592, 0.462, 1.061, 1.344 and 0.888, respectively; the LPs of upstream and downstream are different. The LPs of the Tutujiliangzi gullies are nearly straight lines and slightly concave, while those of the Shadi village gullies are convex and nearly straight lines. The knickpoints and step-pools in Shadi village are more developed, while the gullies in Tutujiliangzi develop more rapidly. This study shows that in counties with similar conditions, these conditions such as temperature and precipitation, local topographic changes, soil properties and vegetation conditions have obvious effects on the development of gullies.

Reactive Astrocytes Display Pro-inflammatory Adaptability with Modulation of Notch-PI3K-AKT Signaling Pathway Under Inflammatory Stimulation.

Astrocytes are major glial cells critical in assisting the function of the central nervous system (CNS), but the functional changes and regulation mechanism of reactive astrocytes are still poorly understood in CNS diseases. In this study, mouse primary astrocytes were cultured, and inflammatory insult was performed to observe functional changes in astrocytes and the involvement of Notch-PI3K-AKT signaling activation through immunofluorescence, PCR, Western blot, CCK-8, and inhibition experiments. Notch downstream signal Hes-1 was clearly observed in the astrocytes, and Notch signal inhibitor GSI dose-dependently decreased the cleaved Notch-l level without an influence on cell viability. Inflammatory insult of lipopolysaccharide plus interferon-γ (LPS+IFNγ) induced an increase in pro-inflammatory cytokines, that is, iNOS, IL-1ß, IL-6, and TNF, at the protein and mRNA levels in activated astrocytes, which was reduced or blocked by GSI treatment. The cell viability of the astrocytes did not show significant differences among different groups. While an increase in MyD88, NF-кB, and phosphor-NF-кB was confirmed, upregulation of PI3K, AKT, and phosphor-AKT was observed in the activated astrocytes with LPS+IFNγ insult and was reduced by GSI treatment. Inhibitor experiments showed that inhibition of Notch-PI3K-AKT signaling activation reduced the pro-inflammatory cytokine production triggered by LPS+IFNγ inflammatory insult. This study showed that the reactive astrocytes displayed pro-inflammatory adaptability through Notch-PI3K-AKT signaling activation in response to inflammatory stimulation, suggesting that the Notch-PI3K-AKT pathway in reactive astrocytes may serve as a promising target against CNS inflammatory disorders.