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Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in May 2024.
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Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in April 2024.
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We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types.
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Epidermólisis Ampollosa Distrófica , Células Madre Pluripotentes Inducidas , Humanos , Masculino , Epidermólisis Ampollosa Distrófica/genética , Epidermólisis Ampollosa Distrófica/metabolismo , Células Madre Pluripotentes Inducidas/metabolismo , Colágeno Tipo VII/genética , Colágeno Tipo VII/metabolismo , Piel/metabolismo , Queratinocitos/metabolismoRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in September 2023.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in August 2023.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in July 2023.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in June 2023.
RESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in April 2023.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non academic institutions in December 2022.
Asunto(s)
Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in September 2022.
Asunto(s)
Medicina Regenerativa , Investigación con Células MadreRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non academic institutions in April 2022.
Asunto(s)
Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in December 2021.
Asunto(s)
Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in November 2021.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in October 2021.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
We examined keratin aggregate formation and the possible mechanisms involved. With this aim, we observed the effect that different ratios between mutant and wild-type keratins expressed in cultured keratinocytes may have on aggregate formation in vitro, as well as how keratin aggregate formation affects the mechanical properties of cells at the cell cortex. To this end we prepared clones with expression rates as close as possible to 25%, 50% and 100% of the EGFP-K14 proteins (either WT or R125P and V270M mutants). Our results showed that only in the case of the 25% EGFP-K14 R125P mutant significant differences could be seen. Namely, we observed in this case the largest accumulation of keratin aggregates and a significant reduction in cell stiffness. To gain insight into the possible mechanisms behind this observation, we extended our previous mathematical model of keratin dynamics by implementing a more complex reaction network that considers the coexistence of wild-type and mutant keratins in the cell. The new model, consisting of a set of coupled, non-linear, ordinary differential equations, allowed us to draw conclusions regarding the relative amounts of intermediate filaments and aggregates in cells, and suggested that aggregate formation by asymmetric binding between wild-type and mutant keratins could explain the data obtained on cells grown in culture.
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Queratinocitos/metabolismo , Queratinas/química , Proteínas Mutantes/química , Agregado de Proteínas , Línea Celular , Simulación por Computador , Colorantes Fluorescentes/química , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Queratinocitos/efectos de los fármacos , Modelos Biológicos , Inhibidores de Proteasoma/farmacología , Agregado de Proteínas/efectos de los fármacosRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in July 2021.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
The latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in June 2021.
Asunto(s)
Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
The latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in May 2021.
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Medicina Regenerativa , Investigación con Células Madre , Industria FarmacéuticaRESUMEN
We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.
Asunto(s)
Epidermólisis Ampollosa Distrófica , Células Madre Pluripotentes Inducidas , Colágeno Tipo VII/genética , Epidermólisis Ampollosa Distrófica/genética , Femenino , Fibroblastos , Humanos , Mutación , PielRESUMEN
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in April 2021.