Trace metal exposure and human health consequences through consumption of market-available Oreochromis niloticus (L.) in Bangladesh.

Using Oreochromis niloticus (L.), commonly known as tilapia, as a model, this study evaluated the exposure of trace metal and their risk assessment on human health. In addition, the status of amino acids, fatty acids, vital elements, and their benefits is also studied. Estimating the nutrient composition of fish muscle is necessary to ensure that it meets the requirements for human health, food regulations, and commercial specifications. The species examined contained appreciable concentrations of amino acids, fatty acid content, and minerals, suggesting that the fish species could be a good source of protein, fat, and minerals. Hazardous heavy metals were found to be lower compared to their corresponding maximum tolerable limits. The order of trace metals is Zn (22,709 µg/kg) > Fe (19,878 µg/kg) > Cu (1261 µg /kg) > Mn (1228 µg/kg) > Cr (474 µg/kg) > Ni (152 µg/kg) As (318 µg /kg) > Pb (281 µg/kg) > Co (24 µg /kg) > Cd (13 µg/kg) > Hg (5 µg/kg); a number of health-related indices, including estimated daily intake (EDI), target hazard quotient (THQ), and hazard index (HI), as well as carcinogenic risk (CR) indices for adult and children, were calculated to evaluate the human health hazard of the heavy metals. The THQ and HI of heavy metals for tilapia are lower than 1, posing a non-carcinogenic threat to human health due to the biomagnifications of these deadly poisonous metals. Principal component, cluster, and correlation analyses delineated the common probabilistic sources of metal contamination origin and significant inter-parameter associations. Although no human health risks for the consumption of tilapia was found, more attention must be paid for the monitoring of Oreochromis niloticus before entering the market.

Fatty acid and amino acid profiles of cheese, butter, and ghee made from buffalo milk.

Objective: The objective was to assess the chemical composition, cholesterol, fatty acid (FAs), and amino acid (AAs) profiles of buffalo cheese, butter, and ghee. Materials and Methods: Buffalo milk (raw) was collected from the Bangladesh Agricultural University (BAU) Dairy Farm, BAU, Mymensingh-2202, Bangladesh. Cheese, butter, and ghee were prepared at the Dairy Chemistry and Technology Laboratory, Department of Dairy Science, BAU, Mymensingh, Bangladesh, and subjected to subsequent analyses. The gross nutritional composition and AAs profile of milk were analyzed prior to the manufacture of cheese, butter, and ghee. The gross nutritional composition of milk and dairy products was analyzed by applying an automated milk analyzer and the Association of Agricultural Chemists techniques, respectively. The cholesterol, FAs, and AAs contents of cheese, butter, and ghee were determined by the Bangladesh Council for Scientific and Industrial Research, Dhaka, Bangladesh. Furthermore, atherogenic and thrombogenic indices were also calculated using reference equations. Results: The results indicated that the buffalo milk is a good source of first-rate nutrients (dry matter: 16.50%, fat: 7.50%, protein: 3.75%). Findings indicated that the butter was significantly rich with (p < 0.05) total solids and fat where higher (p > 0.05) protein, carbohydrate, and minerals were found in cheese. The saponification, Reichert-Meissl, Polenski, and Kirschner values of buffalo ghee were found to be 225, 30, 1.2, and 25, respectively. A significant (p < 0.05) variation was found in the cholesterol content of buffalo cheese, butter, and ghee. Butter and ghee had 40.14 and 39.57 mg more cholesterol, respectively, than cheese. The results revealed identical FA profiles except for C24:0 among the three dairy products where the major FA compositions were C4:0, C14:0, C16:0, and C18:0 and C18:1 cis-9. The atherogenicity index and thrombogenicity index of cheese, butter, and ghee were statistically similar (p > 0.05). Butter was found with the most conducive anti-atherogenic and anti-thrombogenic characteristics due to lower saturated and higher polyunsaturated FAs. However, all the AAs concentrations were statistically higher (p < 0.05) in cheese than in butter and ghee. Conclusion: To conclude, buffalo cheese is superior to butter and ghee as regards nutrient density, but consumers can choose other foods based on their choice.

Variations in fatty acid and amino acid profiles of doi and rasomalai made from buffalo milk.

OBJECTIVE: This study investigated and compared the chemical composition, cholesterol content, fatty acid (FA), and amino acid (AA) profiles of doi and rasomalai made from buffalo milk. MATERIALS AND METHODS: Bangladesh Agricultural University Dairy Farm, Mymensingh-2202, Bangladesh was the source of raw buffalo milk. Then, doi and rasomalai were produced and analyzed. Prior to the production of doi and rasomalai, the gross composition and AAs of milk were evaluated. Milk and dairy products were evaluated for gross composition using an automated milk analyzer and the Association of Agricultural Chemists techniques, respectively. At the Bangladesh Council for Scientific and Industrial Research, Dhaka, Bangladesh, the cholesterol, FA, and AA levels of doi and rasomalai were determined. Additionally, atherogenic and thrombogenic indices were determined using established equations. RESULTS: The results indicated that the majority of the proximate components were significantly greater (p < 0.05) in rasomalai than in doi. Rasomalai had 3.64 mg more cholesterol (p > 0.05) than doi. The FA profile was identical across doi and rasomalai with the exception of oleic acid (C18:1cis-9), which was 1.50% greater (p < 0.05) in rasomalai. The atherogenicity index was found to be statistically higher in doi than in rasomalai (p > 0.05). Similarly, the thrombogenic index was found to be significantly higher (p > 0.05) in doi (1.98) when compared to the rasomalai (1.92). The concentrations of all AAs were found to be quantitatively higher in doi than in rasomalai (p > 0.05). CONCLUSION: The conclusion is that buffalo milk rasomalai appears to have a higher nutritional density than buffalo milk doi.