Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 36
Filtrar
1.
Int J Dermatol Venereol ; 5(3): 132-139, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-36199677

RESUMEN

By assessing and comparing the phenotypic changes on the stepwise acquisition of fluconazole resistant Candida albicans isolates, we could find and describe the relationship between drug resistance and biofilm formation ability in a series of clonal strains. Methods: We performed antifungal susceptibility of five drugs (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) to further verify the antifungal activity of the six isolates in vitro. Then we combined hyphal formation assay, cell surface hydrophobicity test positively related to adherence ability, and biofilm assays in vitro to observe and compare the phenotypic characteristics of our six clonal strains. Results: Biofilm capability is enhanced for four drug- intermediate strains, whereas the initial susceptible strain and the final resistant strain are both poor in adherence, hyphal growth and biofilm formation. Conclusions: It was suggested that the biofilm formation ability were not absolutely related to the degree of fluconazole resistance.

2.
Front Cell Infect Microbiol ; 12: 895329, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35770068

RESUMEN

Background: Due to more attentions paid to melanized fungi over the past few decades and under the background of the global coronavirus disease 2019 pandemic (COVID-19) the fact that the virus itself and the immunosuppressive agents such as glucocorticoids can further increase the risk of infections of deep mycoses, the number of patients with phaeohyphomycosis (PHM) has a substantial increase. Their spectrum is broad and the early diagnosis and treatments are extremely sticky. This study aims to more comprehensively understand the clinical features of phaeohyphomycosis in China over 35 years and to establish a more applicable systematical classification and severity grades of lesions to guide treatments and prognosis. Methods: We reviewed 174 cases of proven phaeohyphomycosis reported in Chinese and English language literature from 1987 to 2021 and we also made the accurate classification definitions and detailed information about the epidemiology, species of clinical dematiaceous fungi, minimum inhibitory concentration values, clinical features, treatments, and prognosis. Results: The mortality of cerebral, disseminated and pulmonary phaeohyphomycosis are 55%, 36%, and 25%. Nearly 19% of patients had poor quality of life caused by the complications such as disability, disfigurements, and blindness. The overall misdiagnosis rate of phaeohyphomycosis was 74%. Moderate to severe rashes are accounting for 82% of subcutaneous phaeohyphomycosis. The areas of the head and face are mostly affected accounting for 16% of severe rashes. Nearly 30% of invasive infections of phaeohyphomycosis are triggered by recurrent lesions. Voriconazole, itraconazole, amphotericin B deoxycholate (AmB-DOC), and terbinafine were most commonly used but diagnosis and treatments of phaeohyphomycosis remain challenging in reality. Conclusions: Our classifications are likely to be more practical and easier to popularize, and there are still also plenty of characteristics in these non-specific lesions. There're no significant variations in cure rates, or death rates between three grades of lesions. But patients with severe rashes have longer courses and lower effective rates.


Asunto(s)
COVID-19 , Feohifomicosis , Antifúngicos/uso terapéutico , Hongos , Humanos , Feohifomicosis/diagnóstico , Feohifomicosis/tratamiento farmacológico , Feohifomicosis/epidemiología , Calidad de Vida , Voriconazol
3.
Mycoses ; 64(6): 624-633, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33586267

RESUMEN

BACKGROUND: Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high-quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. OBJECTIVES: The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. METHODS: Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA-seq) technology. RESULTS: Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH-responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. CONCLUSION: In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.


Asunto(s)
Genoma Fúngico , Trichophyton/genética , Virulencia/genética , Arthrodermataceae/genética , Arthrodermataceae/aislamiento & purificación , Perfilación de la Expresión Génica , Genes Fúngicos , Humanos , Queratinas/metabolismo , Tiña Favosa/microbiología , Trichophyton/metabolismo
4.
Mycoses ; 63(11): 1235-1243, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33090565

RESUMEN

BACKGROUND: In China, the prevalence of superficial fungal infections of the foot is high and recurrence is common. However, a prospective, large-scale and multicentre study on the aetiology of superficial fungal infections of the foot is still lacking. OBJECTIVES: To study the epidemiology of aetiological agents of superficial fungal infections of the foot in urban outpatients in mainland China, as well as to understand the aetiology features of the pathogenic agent. METHODS: The study was designed as a multicentre, prospective epidemiological survey. A total of 1704 subjects were enrolled from seven geographical areas in mainland China. For each subject, one mycological sample and one bacterial sample were collected. KOH wet mount examination and culture were performed at local laboratories. The bacterial results were only reported in those with positive mycology. Further morphological identification and, if necessary, molecular biological identification were conducted in a central laboratory. RESULTS: Of 1704 enrolled subjects, 1327 (77.9%) subjects had positive fungal culture results. The incidence of dermatophytes, yeasts and moulds was 90.1%, 8.1% and 1.1%, respectively. The most frequently isolated aetiological agent (fungus) was Trichophyton rubrum. Moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections. The most frequently isolated bacterial genus in patients was Staphylococcus. CONCLUSION: This study prospectively investigated the clinical and mycological features of human dermatophytosis in mainland China. T rubrum was the most frequently isolated fungus, and moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections.


Asunto(s)
Dermatomicosis , Pie/microbiología , Adulto , Arthrodermataceae/aislamiento & purificación , Arthrodermataceae/patogenicidad , China/epidemiología , Dermatomicosis/epidemiología , Dermatomicosis/etiología , Dermatomicosis/patología , Femenino , Pie/patología , Hongos/aislamiento & purificación , Hongos/patogenicidad , Humanos , Incidencia , Masculino , Micosis/epidemiología , Micosis/etiología , Micosis/patología , Pacientes Ambulatorios , Prevalencia , Estudios Prospectivos , Levaduras/aislamiento & purificación , Levaduras/patogenicidad
5.
Med Mycol Case Rep ; 27: 72-76, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32071849

RESUMEN

Onychomycosis has been reported to be mainly caused by dermatophytes. Recently, more attention has been paid to yeast for its increasing morbidity, especially the candida specices. Here we reported a fingernail infection caused by Pichia guilliermondii, the sexual reproduction period of Candida guilliermondii. Itraconazole was used for three courses, and the patient achieved improvement without any significant side-effects. This might be the first onychomycosis case of Candida guilliermondii.

6.
Ying Yong Sheng Tai Xue Bao ; 31(8): 2793-2803, 2020 Aug.
Artículo en Chino | MEDLINE | ID: mdl-34494803

RESUMEN

To understand the relationships among dominant species of cephalopods in offshore nor-thern South China Sea, we examined the niche characteristics of these dominant species from both spatial and temporal dimensions using the index of relative importance (IRI), the niche breadth and overlap indices based on fishery resources data from the bottom-trawl survey for four seasons during 2014-2015. The results showed that five dominant species of cephalopods were recorded for four seasons, including Loligo edulis, L. chinensis, L. beka, Sepia esculenta, and L. duvaucelii. The first two species were shared by all seasons. Compared with historical data, the composition of dominant cephalopods species had changed. The cephalopods resource exhibited obvious temporal and spatial variations. Stock density was higher in the sea area extending from the southern Hainan Island to eastern Guangdong Province than that in Beibu Gulf. The seasonal variation was characte-rized by the largest in summer but the smallest in winter. The temporal and spatial niche analysis showed that there was inconsistent in the order between temporal and spatial niche breadths for domi-nant species. L. edulis (1.32) and L. chinensis (3.90) occupied the largest temporal and spatial niche breadths, respectively. The smallest of temporal and spatial niche breadths were shown for S. esculenta (0.98) and L. duvaucelii (2.04), respectively. Though the temporal niche overlap was numerically larger than the spatial niche overlap, both of them had higher values in interspecies among L. edulis, L. chinensis, L. beka, and the lower overlap for the species pairs between L. duvaucelii and other species. The result of correlation analysis suggested that niche breadth exhibited a significant negative correlation with variation in abundance on both temporal and spatial scales. The ecological niche could reflect the tempo-spatial changes of species resource, which enriched the traditional methods of fishery communities.


Asunto(s)
Cefalópodos , Animales , China , Ecosistema , Explotaciones Pesqueras , Estaciones del Año
7.
Mol Biol Rep ; 46(6): 5867-5874, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31407245

RESUMEN

In the last two decades, with the wide use of azoles, antifungal resistance among Candida parapsilosis has considered a matter of concern worldwide. The aim of this study is to evaluate the antifungal potentials of tetrandrine (TET) alone and in combination with fluconazole (FLC)/voriconazole (VRC) against C. parapsilosis. Susceptibility tests were performed by microdilution method, checkerboard assay, time-kill test, spot assay. Subsequently, rhodamine 6G efflux test and the expressions of transporter related genes, namely CDR1 and MDR1 for C. parapsilosis were analyzed by qRT-PCR. The susceptibility test showed that TET presented strong synergism with FLC and VRC with fractional inhibitory concentration index (FICI) in a range of 0.094-0.562. The susceptibility results were also confirmed by spot assay and time-kill studies. With TET treatment, a vast quantity of rhodamine 6G could not be pumped out from the cells as considerably intracellular red fluorescence was accumulated. Meanwhile, the expressions of efflux-associated genes presented varying degrees of inhibition. These results indicated that TET was a decent antifungal synergist to promote the antifungal efficacy of FLC/VRC, and the underlying antifungal mechanism might be associated with the inhibition of efflux pump and the elevation of intracellular drug content.


Asunto(s)
Antifúngicos/farmacología , Bencilisoquinolinas/farmacología , Candida parapsilosis/efectos de los fármacos , Fluconazol/farmacología , Voriconazol/farmacología , Farmacorresistencia Fúngica , Sinergismo Farmacológico , Proteínas Fúngicas , Pruebas de Sensibilidad Microbiana
9.
Mycopathologia ; 184(2): 309-313, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30666543

RESUMEN

We report a case of eczema-like cutaneous mucormycosis caused by Rhizopus arrhizus. A 4-year-old child was presented to our hospital with a history of gradually enlarging papule and plaque in the periumbilical area for nearly 4 years since 2 weeks after his birth, and it has been misdiagnosed as eczema for nearly 3 years. Based on histopathology examination, the fungus culture test and DNA sequencing, it was revealed that R. arrhizus should be the responsible fungus for skin infection. The patient was successfully cured by combination of intravenous drip and percutaneous injection amphotericin B for nearly 3 months, and no recrudescence was seen during a follow-up of 6-month observation.


Asunto(s)
Anfotericina B/administración & dosificación , Antifúngicos/administración & dosificación , Dermatomicosis/diagnóstico , Dermatomicosis/patología , Mucormicosis/diagnóstico , Mucormicosis/patología , Rhizopus/aislamiento & purificación , Preescolar , Dermatomicosis/tratamiento farmacológico , Eccema/patología , Histocitoquímica , Humanos , Infusiones Intravenosas , Inyecciones , Masculino , Técnicas Microbiológicas , Mucormicosis/tratamiento farmacológico , Análisis de Secuencia de ADN , Resultado del Tratamiento
10.
Front Microbiol ; 9: 1025, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29875745

RESUMEN

Previous work has explored link between mitochondrial biology and fungal pathogenicity in F1Fo-ATP synthase in Candida albicans. In this work we have detailed the more specific roles of the F1Fo-ATP synthase ß subunit, a key protein subunit of F1Fo-ATP synthase. The ability to assimilate alternative carbons in glucose-limited host niches is known to be a critical factor for infection caused by opportunistic pathogens including C. albicans. The function of the F1Fo-ATP synthase ß subunit was characterized through the construction of an ATP2 gene null mutant (atp2Δ/Δ) and the gene-reconstituted strain (atp2Δ/ATP2) in order to understand the link between carbon metabolism and C. albicans pathogenesis. Cell growth, viability, cellular ATP content, mitochondrial membrane potential (ΔΨm), and intracellular ROS were compared between null mutant and control strain. Results showed that growth of the atp2Δ/Δ mutant in synthetic medium was slower than in complex medium. However, the synthetic medium delayed the onset of reduced cell viability and kept cellular ATP content from becoming fully depleted. Consistent with these observations, we identified transcriptional changes in metabolic response that activated other ATP-generating pathways, thereby improving cell viability during the initial phase. Unlike glucose effects, the atp2Δ/Δ mutant exhibited an immediate and sharp reduction in cell viability on non-fermentable carbon sources, consistent with an immediate depletion of cellular ATP content. Along with a reduced viability in non-fermentable carbon sources, the atp2Δ/Δ mutant displayed avirulence in a murine model of disseminated candidiasis as well as lower fungal loads in mouse organs. Regardless of the medium, however, a decrease in mitochondrial membrane potential (ΔΨm) was found in the atp2Δ/Δ mutant but ROS levels remained in the normal range. These results suggest that the F1Fo-ATP synthase ß subunit is required for C. albicans pathogenicity and operates by affecting metabolic flexibility in carbon consumption.

11.
Mycopathologia ; 183(2): 445-449, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29086142

RESUMEN

We report a case of primary cutaneous mucormycosis caused by Mucor irregularis. A 66-year-old man was presented to our hospital with a history of gradually enlarging plaque on the right leg for about a year. The identification of pathogen based on the fungus morphology and DNA sequencing revealed M. irregularis as the responsible fungus for skin lesion. The lesion was removed incidentally by a surgery procedure, and no recrudescence was seen during a follow-up of 24-month observation.


Asunto(s)
Dermatomicosis/diagnóstico , Dermatomicosis/cirugía , Mucor/aislamiento & purificación , Mucormicosis/diagnóstico , Mucormicosis/cirugía , Anciano , Dermatomicosis/patología , Histocitoquímica , Humanos , Masculino , Técnicas Microbiológicas , Microscopía , Mucor/clasificación , Mucor/citología , Mucor/genética , Mucormicosis/patología , Análisis de Secuencia de ADN , Resultado del Tratamiento
12.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-666463

RESUMEN

OBJECTIVE To investigate the protective effect of Codonopsis Pilosula Polysaccharide (CPPS) on improving of the memory consolidation disorder induced by Cycloheximide and its possible mechanisms in mice. METHODS The mice was divided into five groups, as normal control group, cycloheximid model group, piracetam positive control group, CPPS 300 mg · kg- 1 group, and CPPS 150 mg·kg-1 group. The mice respectively were given saline, piracetam, and CPPS for 15 d. The memory consolidation disorder model in mice was established by ip. Cyclohexylamine, and orally administered CPPS(300 mg·kg-1 or 150 mg·kg-1) every day. Then experimental groups were subjected Morris Water Maze test. Western blotting analysis were used to analysis the expression of CaMKⅡ/CREB signaling pathways. RESULTS Morris water maze experiment showed that cyclohexylamine can cause memory consolidation disorder(P<0.01), and giving piracetam and CPPS (300 mg · kg- 1) can improve spatial memory impairment in mice(P<0.05, P<0.01). Western blotting experiment results show that compared with normal control group, CaMKⅡ and CREB contents of brain in model group mice had significant decreased(P<0.001); Compared with model group, CaMK Ⅱ and CREB contents of brain tissue in piracetam and CPPS groups increased significantly(P<0.05,P<0.01,P<0.001). CONCLUSION Cyclo?heximide can induce the memory consolidation disorder, and its effect in mice related to CaMK/CREB signaling pathways. CPPS can improved this memory disorder by influence CaMKⅡ/CREB signaling pathways.

13.
Drug Des Devel Ther ; 7: 669-80, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23930073

RESUMEN

PURPOSE: The aim of the present study was to investigate the immune response induced by Mycobacterium marinum infection in vitro and the potential of M. marinum as an immunotherapy for M. tuberculosis infection. METHODS: The potential human immune response to certain bacillus infections was investigated in an immune cell-bacillus coculture system in vitro. As a potential novel immunotherapy, M. marinum was studied and compared with two other bacilli, Bacillus Calmette-Guérin (BCG) and live attenuated M. tuberculosis. We examined the changes in both the bacilli and immune cells, especially the time course of the viability of mycobacteria in the coculture system and host immune responses including multinuclear giant cell formation by Wright-Giemsa modified staining, macrophage polarization by cell surface antigen expression, and cytokines/chemokine production by both mRNA expression and protein secretion. RESULTS: The M. marinum stimulated coculture group showed more expression of CD209, CD68, CD80, and CD86 than the BCG and M. tuberculosis (an attenuated strain, H37Ra) groups, although the differences were not statistically significant. Moreover, the M. marinum group expressed more interleukin (IL)-1B and IL-12p40 on day 3 (IL-1B: P = 0.003 and 0.004, respectively; IL-12p40: P = 0.001 and 0.011, respectively), a higher level of CXCL10 on day 1 (P = 0.006 and 0.026, respectively), and higher levels of chemokine (C-X-C motif) ligand (CXCL) 8 and chemokine (C motif) ligand (XCL) 1 on day 3 (CXCL8: P = 0.012 and 0.014, respectively; XCL1: P = 0.000 and 0.000, respectively). The M. marinum stimulated coculture group also secreted more tumor necrosis factor (TNF)-α, IL-1ß, and IL-10 on day 1 (TNF-α: P = 0.000 and 0.000, respectively; IL-1ß: P = 0.000 and 0.000, respectively; IL-10: P = 0.002 and 0.019, respectively) and day 3 (TNF-α: P = 0.000 and 0.000, respectively; IL-1ß: P = 0.000 and 0.001, respectively; IL-10: P = 0.000 and 0.000, respectively). In addition, the colony-forming units (an index of viability) of M. marinum in the M. marinum stimulated coculture group was significantly less than that of BCG and H37Ra in their corresponding bacillus stimulated groups (P = 0.037 and 0.013, respectively). CONCLUSION: Our results indicated that M. marinum could be a potentially safe and effective immunotherapy.


Asunto(s)
Mycobacterium marinum/inmunología , Tuberculosis/terapia , Agregación Celular , Quimiocinas/genética , Preescolar , Técnicas de Cocultivo , Citocinas/genética , Granuloma/microbiología , Humanos , Inmunoterapia , Lactante , Recién Nacido , ARN Mensajero/análisis
14.
PLoS One ; 8(6): e65065, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23798999

RESUMEN

BACKGROUND: In recent years, the fungal infectious disease zygomycosis has increased in incidence worldwide, especially among the immunodeficient population. Despite the rates of zygomycosis-related death and deformation being very high, the mechanism(s) by which the fungal pathogens cause these severe manifestations remain unknown. METHODS: Using the associated Rhizomucor variabilis species, which can selectively induce cutaneous zygomycosis in otherwise healthy individuals, we investigated the host mechanisms of infection-related responses, including cytokine and chemokine expression as well as contributions of particular T cell subsets. siRNA specifically targeting IL-22,IL-17 and IFN-γ were used to down-regulate expression of those molecules. RESULTS: In mouse models of infection, IL-22 was implicated in development of Rhizomucor spp.-induced skin lesions. In cultured human peripheral blood monocytes, R. pusilluscan, which is often found in immunodeficient patients, induced the production of IL-22, while R. variabilis did not. Moreover, Rhizomucor spp.-induced secretion of Il-22 from CCR6(+)CCR4(+)CCR10(+) cells was down-regulated by knockdown of IL-22 related signaling receptors, RORC and ARH. CONCLUSION: Our data strongly suggest that avoidance of IL-22 may be one mechanism by which mucor species produce morbidity and mortality in infected individuals.


Asunto(s)
Interleucinas/fisiología , Mucormicosis/inmunología , Rhizomucor/inmunología , Animales , Secuencia de Bases , Linfocitos T CD4-Positivos/inmunología , Citocinas/biosíntesis , Cartilla de ADN , Modelos Animales de Enfermedad , Citometría de Flujo , Interleucinas/biosíntesis , Interleucinas/genética , Ratones , Ratones Endogámicos BALB C , Mucormicosis/microbiología , ARN Interferente Pequeño/genética , Interleucina-22
16.
Mycopathologia ; 176(1-2): 101-5, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23591621

RESUMEN

We present the first case of phaeohyphomycosis caused by Rhinocladiella basitona (R. basitona) in China and describe the mycological characteristics of this pathogen. A 11-year-old girl was presented with plaque on her face for 3 years. Diagnosis was based on histopathology, mycology, and molecular identification. The patient was treated with terbinafine and itraconazole. This case is the second of phaeohyphomycosis caused by R. basitona in the world (previously belonging to Geniculosporium).


Asunto(s)
Ascomicetos/clasificación , Ascomicetos/aislamiento & purificación , Feohifomicosis/diagnóstico , Feohifomicosis/microbiología , Antifúngicos/uso terapéutico , Niño , China , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Cara/patología , Femenino , Histocitoquímica , Humanos , Itraconazol/uso terapéutico , Técnicas Microbiológicas , Técnicas de Diagnóstico Molecular , Datos de Secuencia Molecular , Naftalenos/uso terapéutico , Feohifomicosis/tratamiento farmacológico , Feohifomicosis/patología , Análisis de Secuencia de ADN , Terbinafina
17.
Braz. j. microbiol ; 43(3): 873-879, July-Sept. 2012. ilus, tab
Artículo en Inglés | LILACS | ID: lil-656647

RESUMEN

Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were differentiated. We evaluated 10 reference strains representing 10 yeast species, among which C. albicans and C. dubliniensis were successfully identified. A total of 56 phenotypically characterized clinical isolates (42 C. albicans isolates and 14 C. dubliniensis isolates) were also investigated for intra-species variability. All tested isolates produced identical RFLP patterns to their respective reference strains except one initially misidentified isolate. Our method offers a simple, rapid and reliable molecular method for the identification of C. albicans and C. dubliniensis.


Asunto(s)
Humanos , Candidiasis , Candida albicans/genética , Candida albicans/aislamiento & purificación , Fenotipo , Polimorfismo Genético , Reacción en Cadena de la Polimerasa/métodos , Métodos , Pacientes , Virulencia
18.
Immunopharmacol Immunotoxicol ; 34(2): 196-204, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21721923

RESUMEN

Many attempts have been made to develop in vitro sensitization tests that employ dendritic cells (DCs), DC-like cell lines or keratinocytes. The aim of the present investigation was to establish a co-culture of THP-1 cells and keratinocytes for evaluation of skin sensitization potential of chemicals. Co-cultures were constructed by THP-1 cells cultured in lower compartments and keratinocytes cultured in upper compartments of cell culture inserts. After 24 h exposure to sensitizers (2, 4-dinitrochlorobenzene, p-phenylenediamine, formaldehyde, nickel sulfate, isoeugenol and eugenol) and non-sensitizers (sodium lauryl sulfate, benzalkonium chloride and lactic acid), the expression of CD86 and CD54 on THP-1 cells were evaluated by flow cytometry, and cell viabilities were determined. The sensitizers induced the augmentation of CD86 and CD54 expression, but the non-sensitizers had no significant effect. Compared with mono-cultures of THP-1 cells, the augmentation of CD86 and CD54 could be detected even at a non-toxic concentration of sensitizers in THP-1 cell/keratinocyte co-cultures. Moreover, isoeugenol was distinguished as a sensitizer in co-cultures, but failed to be identified in mono-cultures. These results revealed that the co-cultures of THP-1 cells and keratinocytes were successfully established and suitable for identifying sensitizers using CD86 and CD54 expression as identification markers.


Asunto(s)
Alternativas a las Pruebas en Animales/métodos , Dermatitis Alérgica por Contacto/inmunología , Haptenos/inmunología , Queratinocitos/inmunología , Monocitos/inmunología , Antígeno B7-2/metabolismo , Compuestos de Benzalconio/farmacología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Técnicas de Cocultivo , Dinitroclorobenceno/inmunología , Dinitroclorobenceno/farmacología , Eugenol/análogos & derivados , Eugenol/inmunología , Eugenol/farmacología , Formaldehído/inmunología , Formaldehído/farmacología , Haptenos/farmacología , Humanos , Molécula 1 de Adhesión Intercelular/metabolismo , Queratinocitos/citología , Ácido Láctico/inmunología , Ácido Láctico/farmacología , Monocitos/citología , Monocitos/metabolismo , Níquel/inmunología , Níquel/farmacología , Fenilendiaminas/inmunología , Fenilendiaminas/farmacología , Sensibilidad y Especificidad , Pruebas Cutáneas/métodos , Dodecil Sulfato de Sodio/farmacología
19.
Braz J Microbiol ; 43(3): 873-9, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24031901

RESUMEN

Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were differentiated. We evaluated 10 reference strains representing 10 yeast species, among which C. albicans and C. dubliniensis were successfully identified. A total of 56 phenotypically characterized clinical isolates (42 C. albicans isolates and 14 C. dubliniensis isolates) were also investigated for intra-species variability. All tested isolates produced identical RFLP patterns to their respective reference strains except one initially misidentified isolate. Our method offers a simple, rapid and reliable molecular method for the identification of C. albicans and C. dubliniensis.

20.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 33(4): 371-4, 2011 Aug.
Artículo en Chino | MEDLINE | ID: mdl-21906443

RESUMEN

OBJECTIVE: To investigate whether Candida albicans-native phospholipomannan (PLM) induce an inflammation response through Toll-like receptor(TLRé2 in human acute monocytic leukemia cell line (THP-1) cells. METHODS: Human THP-1 monocytes were challenged with PLM in vitro. The mRNA expressions of TLR2, TLR4, proinflammatory cytokine [interleukin(IL)-6], and chemokine (IL-8) were assayed by real time reverse transcription polymerase chain reaction. The secretions of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay. The expression of TLR2 was analyzed with Western blot. RESULTS: PLM increased the mRNA expressions and secretions of proinflammatory cytokines (IL-6) and chemokines (IL-8) in THP-1 cells (all P=0.0000). PLM up-regulated the mRNA and protein levels of TLR2 (P=0.0000), whereas the mRNA level of TLR4 was not altered. PLM hydrolyzed with ß-D-mannoside manno hydrolase failed to induce gene and protein expressions of TLR2, IL-6, and IL-8. Anti-TLRS-neutralizing antibody blocked the PLM-induced secretions of IL-6 and IL-8 in THP-1 cells (P = 0.0003, P = 0.0010). CONCLUSION: Canidada albicans-native PLM may contribute to the inflammatory responses during Candida infection in a TLR2-dependent manner.


Asunto(s)
Candida albicans/química , Glucolípidos/farmacología , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Monocitos/efectos de los fármacos , Células Cultivadas , Humanos , Monocitos/inmunología , Monocitos/metabolismo , Receptor Toll-Like 2/metabolismo , Receptor Toll-Like 4/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...