RESUMEN
Magnesium oxychloride cement (abbreviated as MOC) was prepared using magnesium residue obtained from Li2CO3 extraction from salt lakes as raw material instead of light magnesium oxide. The properties of magnesium residue calcined at different temperatures were researched by XRD, SEM, LSPA, and SNAA. The preparation of MOC specimens with magnesium residue at different calcination temperatures (from 500 °C to 800 °C) and magnesium chloride solutions with different Baume degrees (24 Baume and 28 Baume) were studied. Compression strength tests were conducted at different curing ages from 3 d to 28 d. The hydration products, microstructure, and porosity of the specimens were analyzed by XRD, SEM, and MIP, respectively. The experimental results showed that magnesium residue's properties, the BET surface gradually decreased and the crystal size increased with increasing calcination temperature, resulting in a longer setting time of MOC cement. Additionally, the experiment also indicated that magnesium chloride solution with a high Baume makes the MOC cement have higher strength. The MOC specimens prepared by magnesium residue at 800 °C and magnesium chloride solution Baume 28 exhibited a compressive of 123.3 MPa at 28 d, which met the mechanical property requirement of MOC materials. At the same time, magnesium oxychloride cement can be an effective alternative to Portland cement-based materials. In addition, it can reduce environmental pollution and improve the environmental impact of the construction industry, which is of great significance for sustainable development.
RESUMEN
Staphylococcus aureus is a major pathogen associated to bovine mastitis and has the ability to form a slow-growing population termed the small colony variants (SCVs). From 20 samples of 5 chronic S. aureus cases, 1 SCV isolate (SCV102) was recovered simultaneously with 1 of 8 S. aureus isolates. SCV102 showed auxotrophy for thymidine and had a slow growth rate. Intracellular persistence in human mammary epithelial cells (HBL100cell line) monolayer revealed that SCV102 isolate had minimal cytopathological effects compared with its parent strains. SCV102 isolate and its parent strain S. aureus 101 indicate similar resistant pattern to four antibiotics. On the contrary, the minimal inhibitory concentrations values for chloramphenicol and sulfadimethoxine were much higher in SCV102 than that of S. aureus 101. To the best of our knowledge this is the first time the isolation of S. aureus SCV102 from a persistent bovine mastitis has been reported in Beijing (China). This study suggests that SCV102 isolate may be an important contributor to persistent bovine mastitis.
Asunto(s)
Antibacterianos/farmacología , Mastitis Bovina/microbiología , Infecciones Estafilocócicas/veterinaria , Staphylococcus aureus/crecimiento & desarrollo , Animales , Antibacterianos/uso terapéutico , Bovinos , China , Cloranfenicol/farmacología , Cloranfenicol/uso terapéutico , Enfermedad Crónica , Recuento de Colonia Microbiana/veterinaria , Células Epiteliales/efectos de los fármacos , Células Epiteliales/microbiología , Femenino , Humanos , Mastitis Bovina/tratamiento farmacológico , Pruebas de Sensibilidad Microbiana/veterinaria , Infecciones Estafilocócicas/tratamiento farmacológico , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/aislamiento & purificación , Sulfadimetoxina/farmacología , Sulfadimetoxina/uso terapéutico , Timidina/metabolismoRESUMEN
The molecular diversity, antibiotic resistance patterns and presence of resistance genes were determined in Staphylococcus aureus isolates from cases of bovine mastitis in a dairy cattle herd in China. Multiple locus variable number tandem repeat analysis was used for molecular typing. Resistance was determined through minimum inhibitory concentrations and resistance genes were detected by PCR. There was low molecular diversity; one predominant strain (type I) accounted for the majority of cases of S. aureus mastitis in the herd and this strain had a high frequency of resistance to penicillin and tetracycline. The most prevalent resistance genes were blaZ, ermC and tetM.