Water and salt recovery from shale gas produced water by vacuum membrane distillation followed by crystallization.

A vacuum membrane distillation (VMD) followed by crystallization (VMD-C) was developed for the recovery of water and salts from shale gas produced water (SGPW). Before VMD, the pretreatment of SGPW with Fenton oxidation-flocculation is applied, with the chemical oxygen demand (COD) concentration reduction of 75% and the total removal of the total suspended solids (TSS), Ca2+, and Mg2+ in SGPW. The pretreatment of SGPW mitigated the membrane fouling in the VMD and effectively prevented the reduction of membrane flux over time. The average flux of the PTFE membrane reached 12.1 kg m-2 h-1 during the separation of the pretreated SGPW at a feed flux of 40 L h-1 and a feed temperature of 40 °C. The rejection rate of the membrane to TDS in SGPW was over 99%. Fresh water with a conductivity of below 20 µs cm-1 was produced by VMD-C. The salts concentrated upstream of the membrane were recovered by a stirring crystallization process. The VMD-C system resulted in a 61% cost savings compared to conventional SGPW treatment.

BACH1 promotes lung adenocarcinoma cell metastasis through transcriptional activation of ITGA2.

BACH1 plays an important role in promoting cancer. This study aims to further verify the relationship between the expression level of BACH1 in lung adenocarcinoma prognosis, as well as the influence of BACH1 expression on lung adenocarcinoma and the potential mechanism. The expression level of BACH1 in lung adenocarcinoma and its relationship with prognosis was evaluated by lung adenocarcinoma tissue microarray analysis combined with bioinformatics approaches. Gene knockdown and overexpression were used to investigate the functions and molecular mechanisms of BACH1 in lung adenocarcinoma cells. The regulatory downstream pathways and target genes of BACH1 in lung adenocarcinoma cells were explored by bioinformatics and RNA sequencing data analysis, real-time PCR, western blot analysis, and cell immunofluorescence and cell adhesion assays. Chromatin immunoprecipitation and dual-luciferase reporter assays were carried out to verify the target gene binding site. In the present study, BACH1 is abnormally highly expressed in lung adenocarcinoma tissues, and high BACH1 expression is negatively correlated with patient prognosis. BACH1 promotes the migration and invasion of lung adenocarcinoma cells. Mechanistically, BACH1 directly binds to the upstream sequence of the ITGA2 promoter to promote ITGA2 expression, and the BACH1-ITGA2 axis is involved in cytoskeletal regulation in lung adenocarcinoma cells by activating the FAK-RAC1-PAK signaling pathway. Our results indicated that BACH1 positively regulates the expression of ITGA2 through a transcriptional mechanism, thereby activating the FAK-RAC1-PAK signaling pathway to participate in the formation of the cytoskeleton in tumor cells and then promoting the migration and invasion of tumor cells.

Optimizing the operation strategy of a combined cooling, heating and power system based on energy storage technology.

Energy storage technology is the key to achieving a carbon emission policy. The purpose of the paper is to improve the overall performance of the combined cooling, heating and power-ground source heat pump (CCHP-GSHP) system by the battery. A new operation strategy (the two-point operation) is proposed by controlling the power generation unit work. The power generation unit has two operation modes of non-operation and rated efficiency operation by the storage electricity battery. The new operation strategy is compared with the traditional CCHP-GSHP that without a battery. The optimization goals include the primary energy saving ratio, the reduction ratio of carbon dioxide emissions, and the annual total cost saving ratio. The independent GSHP system is used as a reference system. Multipopulation genetic algorithms are selected to achieve the problem of optimization. A hotel building is selected for a case study. The optimal configuration of the coupling system is computed following the electric load strategy. Finally, the results show that the CCHP-GSHP system has a better performance under the new operation strategy compared with the traditional CCHP-GSHP (the primary energy saving ratio increases by 5.5%; the annual carbon dioxide emission reduction ratio increases by 1%; the annual total cost reduction ratio increases by 5.1%). This paper provides reference and suggestions for the integration and operation strategy of CCHP-GSHP in the future.

Long noncoding RNA CDKN2B-AS1 silencing protects against esophageal cancer cell invasion and migration by inactivating the TFAP2A/FSCN1 axis.

Esophageal cancer (EC) is the most aggressive malignancy in the gastrointestinal tract. Long noncoding RNA cyclin-dependent kinase inhibitor 2 B antisense RNA 1 (CDKN2B-AS1) is implicated in EC development. However, the specific mechanisms involved remain poorly defined. Therefore, this research aimed to explore the mechanism of action of CDKN2B-AS1 in EC. Quantitative real-time polymerase chain reaction was conducted to measure CDKN2B-AS1 expression in EC cells and western blotting was utilized to evaluate transcription factor AP-2 alpha (TFAP2A) and fascin actin-bundling protein 1 (FSCN1) expression. After gain-of-function and loss-of-function assays, cell proliferation, migration, invasion, apoptosis, and apoptosis-related protein expression were assessed using cell counting kit-8, scratch tests, Transwell assays, flow cytometry, and western blotting, respectively. The binding relationship between CDKN2B-AS1 and TFAP2A was assessed by RNA immunoprecipitation and RNA pull-down assays. The binding relationship between TFAP2A and FSCN1 was evaluated using dual-luciferase reporter and chromatin immunoprecipitation assays. Tumor xenografts from nude mice were used for in vivo verification. CDKN2B-AS1, TFAP2A, and FSCN1 were upregulated in EC cells. Mechanistically, CDKN2B-AS1 transcriptionally activated FSCN1 by recruiting TFAP2A to the FSCN1 promoter. Silencing CDKN2B-AS1 or TFAP2A suppressed EC cell proliferative, migrating, and invasive properties and augmented apoptosis. TFAP2A was bound to CDKN2B-AS1 and the FSCN1 promoter. Overexpression of TFAP2A or FSCN1 abolished the effects of CDKN2B-AS1-silencing on EC cell function. CDKN2B-AS1 silencing curtailed tumorigenesis in nude mice, which was nullified by the upregulation of TFAP2A or FSCN1. Our findings demonstrated the antioncogenic effects of silencing CDKN2B-AS1 in EC through inactivation of the TFAP2A/FSCN1 axis.

Constructing 2D/2D ultrathin Ti3C2/SnS2 Schottky heterojunctions toward efficient tetracycline degradation.

In this article, a novel 2D/2D ultrathin Ti3C2/SnS2 Schottky heterojunctions have been prepared via a facile hydrothermal process. The properties of the heterojunction were fully characterized. The photocatalytic degradation performance of composites was examined by photo-degradation of tetracycline hydrochloride (TC-HCL) under visible light irradiation. Compared with single SnS2, 3% Ti3C2/SnS2 displayed the better performance, the removal rate of TC-HCL reached 87.7% and the kinetic rate constant (k) of the optimal 3% Ti3C2/SnS2 composite was about 2.7 times of that of bare SnS2. The improved photocatalytic activity of Ti3C2/SnS2 is ascribed to the formation of 2D/2D Schottky heterojunction, which promotes the spatial charge separation and increases the surface reactive sites.

PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.

BACKGROUND: The roles of Polypyrimidine tract-binding protein 3 (PTBP3) in regulating lung squamous cell carcinoma (LUSC) cells progression is unclear. The aim of this study was to investigate the role of PTBP3 in LUSC. METHODS: Expression and survival analysis of PTBP3 was firstly investigated using TCGA datasets. Quantitative reverse transcription PCR and Western blot were performed to detect PTBP3 expression in clinical samples. Moreover, cell counting kit 8 (CCK-8) assays, colony formation assays and in vivo tumor formation assays were used to examine the effects of PTBP3 on LUSC cell proliferation. RNA-sequence and analysis explores pathways regulated by PTBP3.Flow cytology was used analyzed cell cycle. Cell cycle-related markers were analyzed by Western blot. RESULTS: PTBP3 was found to be overexpressed in LUSC tissues compared with normal tissues. High PTBP3 expression was significantly correlated with poor prognosis. In vitro and vivo experiments demonstrated that PTBP3 knockdown caused a significant decrease in the proliferation rate of cells. Bioinformatics analysis showed that PTBP3 involved in cell cycle pathway regulation in LUSC. Furthermore, PTBP3 knockdown arrested cell cycle progression at S phase via decreasing CDK2/Cyclin A2 complex. In addition, downregulation of PTBP3 significantly decreased the expression of CDC25A. CONCLUSIONS: Our results suggest that PTBP3 regulated LUSC cell proliferation via cell cycle and might be a potential target for molecular therapy of LUSC.

Hsa_circ_0005576 promotes osimertinib resistance through the miR-512-5p/IGF1R axis in lung adenocarcinoma cells.

Osimertinib is a third-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) for lung adenocarcinoma (LUAD) harboring activating mutations, but patients ultimately develop acquired resistance. Circular RNAs are involved in EGFR-TKI resistance, while the role of hsa_circ_0005576 in the osimertinib resistance of LUAD remains unknown. In this study, we demonstrated that hsa_circ_0005576 could facilitate osimertinib-resistant LUAD cells. Briefly, knockdown of hsa_circ_0005576 not only suppressed the proliferation and promoted the apoptosis of resistant LUAD cells, but also increased their sensitivity to osimertinib. Mechanistically, hsa_circ_0005576, serving as an miRNA sponge, could directly interact with miR-512-5p and subsequently upregulate the miR-512-5p-targeted insulin-like growth factor 1 receptor. Rescue assays indicated that miR-512-5p inhibition could reverse the effects of hsa_circ_0005576 knockdown in LUAD cells resistant to osimertinib. Overall, our study revealed that hsa_circ_0005576 regulates proliferation and apoptosis through miR-512-5p/IGF1R signaling, which contributes further to the resistance of LUAD cells to osimertinib. In addition, this study provides a novel insight into the mechanisms underlying osimertinib resistance of LUAD.

Preparation and characterization of myristic acid/expanded graphite composite phase change materials for thermal energy storage.

Myristic acid/expanded graphite (MA/EG) composite phase-change material (CPCM) was prepared by absorbing liquid MA (as the PCM) into EG (as the supporting material). Its chemical structure, microstructure, and thermal properties were characterized and studied. In the MA/EG CPCM, the largest mass content of MA was 93.5% by using the diffusion-exudation circle method for the first time. Fourier transform infrared spectroscopy (FTIR) analysis indicated that the MA and EG were a pure physical mixture of which the structure does not change, and they undergo no chemical reaction. Differential-scanning-calorimetry (DSC) analysis results showed that the melting and freezing temperatures of the MA/EG CPCM were 53.3 and [Formula: see text], respectively, and the melting and freezing latent heats were 189.5 and 187.8 J/g, respectively. After several heat-cycle accelerations, the material still had good thermal-energy-storage effect. MA/EG CPCM thermoconductivity was greatly enhanced after adding EG, and the results of thermal-storage/-release experiments indicated that the thermal-storage and -release ratios of the MA/EG phase-change unit was greatly improved when compared with that of MA. These results indicated that the MA/EG CPCM was a suitable low-temperature thermal-energy-storage material.

>100 W GHz femtosecond burst mode all-fiber laser system at 1.0 µm.

In this work, we report a >100 W femtosecond (fs) burst mode all-fiber laser system at 1.0 µm that operates at an intra-burst repetition rate of up to 1.2 GHz. This fiber laser system provides the highest output power that has been reported so far for GHz fs fiber lasers, to the best of our knowledge. In addition to the superior output power, this fiber laser system also shows a promising overall figure of merit, specifically in terms of pulse width (473 fs), long-term reliability (<0.67% power fluctuation) and system compactness (all-fiber configuration). We anticipate that this all-fiber laser system can be a promising ultrafast laser source for these applications requiring fs pulses with both high average power and high repetition rate, such as micromachining, bioimaging and frequency metrology.

Gain-guided soliton: Scaling repetition rate of passively modelocked Yb-doped fiber lasers to 12.5 GHz.

Fundamental repetition rates of 3.1 GHz, 7.0 GHz, and 12.5 GHz in passively modelocked Yb-doped fiber lasers are demonstrated. To the best of our knowledge, the fundamental repetition rate of 12.5 GHz is the highest value for 1.0 µm mode-locked fiber lasers. The mode-locked oscillator has a peak wavelength of 1047.5 nm and a pulse duration of 1.9 ps. The repetition rate signal has a signal-to-noise ratio of 57 dB. The peak wavelength of mode-locked spectra gradually makes a blue-shift and the modelocked threshold power increases with an increase in pulse repetition rate. Furthermore, in contrast to most of the all-normal-dispersion mode-locked fiber lasers, the present linear resonator (e.g., length < 1 cm) allows the buildup of gain-guided soliton without any filter effect. To unveil the underlying pulse shaping mechanism, a combined model comprising dynamic rate equations and the generalized nonlinear Schrödinger equation is established. Surprisingly, an essential gain filtering effect, which is contributed by a 26-nm gain bandwidth, is revealed and can verify the gain-guided pulse dynamics. Moreover, the pulse build-up in temporal and frequency domain, like spectral evolution and gain bandwidths, is numerically carried out in detail.

High-power 2 GHz fs pulsed all-fiber amplified laser system at 2.0 µm.

High-power femtosecond (fs)-pulsed all-fiber lasers operating at high repetition rates are highly demanded for various applications, including laser micromachining, nonlinear optical imaging, high-speed optical sampling, arbitrary waveform generation, and frequency metrology. However, their performance has long been limited by either the average power, repetition rate, pulse width, or compactness, which prevent practical applications. In this work, we report a high repetition rate fs-pulsed all-fiber laser at 2.0 µm that so far provides the best performance metrics, to the best of our knowledge, i.e., ∼2GHz fundamental repetition rate, 126 fs pulse width, ∼8W average power, and all-fiber configuration. We anticipate that this laser can be a promising fs-pulsed fiber laser source for applications requiring a GHz repetition rate.

Bioethanol production in vacuum membrane distillation bioreactor by permeate fractional condensation and mechanical vapor compression with polytetrafluoroethylene (PTFE) membrane.

A vacuum membrane distillation bioreactor (VMDBR) by permeate fractional condensation and mechanical vapor compression with PTFE membrane was developed for bioethanol production. Cell concentration of 11.5 g/L, glucose consumption rate of 5.2 g/L/h and ethanol productivity of 2.3 g/L/h could be obtained with fermentation continues lasting for 140 h. Membrane flux of over 10 kg/m2/h could be obtained for model solution separation. Higher temperature and flow rate could promote membrane separation. Membrane flux could be reduced to about 2000 g/m2/h with fermentation proceeding owing to the deposited cell on membrane surface. The membrane separation performance could be resumed by water rinse. High ethanol concentration of 421 g/L could be obtained by permeate fractional condensation with the process separation factor increased to 19.2. Energy of only 14 MJ/kg was required in VMDBR and the energy consumption would be reduced further if the compressed vapor could be used to heat the feed.