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1.
Nat Commun ; 15(1): 4578, 2024 May 29.
Artículo en Inglés | MEDLINE | ID: mdl-38811586

RESUMEN

Modulation of the cervix by steroid hormones and commensal microbiome play a central role in the health of the female reproductive tract. Here we describe organ-on-a-chip (Organ Chip) models that recreate the human cervical epithelial-stromal interface with a functional epithelial barrier and production of mucus with biochemical and hormone-responsive properties similar to living cervix. When Cervix Chips are populated with optimal healthy versus dysbiotic microbial communities (dominated by Lactobacillus crispatus and Gardnerella vaginalis, respectively), significant differences in tissue innate immune responses, barrier function, cell viability, proteome, and mucus composition are observed that are similar to those seen in vivo. Thus, human Cervix Organ Chips represent physiologically relevant in vitro models to study cervix physiology and host-microbiome interactions, and hence may be used as a preclinical testbed for development of therapeutic interventions to enhance women's health.


Asunto(s)
Cuello del Útero , Interacciones Microbiota-Huesped , Inmunidad Innata , Microbiota , Humanos , Femenino , Cuello del Útero/microbiología , Cuello del Útero/inmunología , Microbiota/inmunología , Interacciones Microbiota-Huesped/inmunología , Gardnerella vaginalis/inmunología , Lactobacillus crispatus/inmunología , Moco/inmunología , Moco/microbiología , Moco/metabolismo , Dispositivos Laboratorio en un Chip
2.
Adv Drug Deliv Rev ; 191: 114542, 2022 12.
Artículo en Inglés | MEDLINE | ID: mdl-36179916

RESUMEN

The surfaces of human internal organs are lined by a mucus layer that ensures symbiotic relationships with commensal microbiome while protecting against potentially injurious environmental chemicals, toxins, and pathogens, and disruption of this layer can contribute to disease development. Studying mucus biology has been challenging due to the lack of physiologically relevant human in vitro models. Here we review recent progress that has been made in the development of human organ-on-a-chip microfluidic culture models that reconstitute epithelial tissue barriers and physiologically relevant mucus layers with a focus on lung, colon, small intestine, cervix and vagina. These organ-on-a-chip models that incorporate dynamic fluid flow, air-liquid interfaces, and physiologically relevant mechanical cues can be used to study mucus composition, mechanics, and structure, as well as investigate its contributions to human health and disease with a level of biomimicry not possible in the past.


Asunto(s)
Modelos Biológicos , Moco , Humanos , Colon , Dispositivos Laboratorio en un Chip , Microbiota , Microfluídica , Moco/fisiología
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