Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats.

Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.

Clinical Implications of Epigenetic Dysregulation in Perinatal Hypoxic-Ischemic Brain Damage.

Placental and fetal hypoxia caused by perinatal hypoxic-ischemic events are major causes of stillbirth, neonatal morbidity, and long-term neurological sequelae among surviving neonates. Brain hypoxia and associated pathological processes such as excitotoxicity, apoptosis, necrosis, and inflammation, are associated with lasting disruptions in epigenetic control of gene expression contributing to neurological dysfunction. Recent studies have pointed to DNA (de)methylation, histone modifications, and non-coding RNAs as crucial components of hypoxic-ischemic encephalopathy (HIE). The understanding of epigenetic dysregulation in HIE is essential in the development of new clinical interventions for perinatal HIE. Here, we summarize our current understanding of epigenetic mechanisms underlying the molecular pathology of HI brain damage and its clinical implications in terms of new diagnostic, prognostic, and therapeutic tools.

A translational murine model of sub-lethal intoxication with Shiga toxin 2 reveals novel ultrastructural findings in the brain striatum.

Infection by Shiga toxin-producing Escherichia coli causes hemorrhagic colitis, hemolytic uremic syndrome (HUS), acute renal failure, and also central nervous system complications in around 30% of the children affected. Besides, neurological deficits are one of the most unrepairable and untreatable outcomes of HUS. Study of the striatum is relevant because basal ganglia are one of the brain areas most commonly affected in patients that have suffered from HUS and since the deleterious effects of a sub-lethal dose of Shiga toxin have never been studied in the striatum, the purpose of this study was to attempt to simulate an infection by Shiga toxin-producing E. coli in a murine model. To this end, intravenous administration of a sub-lethal dose of Shiga toxin 2 (0.5 ηg per mouse) was used and the correlation between neurological manifestations and ultrastructural changes in striatal brain cells was studied in detail. Neurological manifestations included significant motor behavior abnormalities in spontaneous motor activity, gait, pelvic elevation and hind limb activity eight days after administration of the toxin. Transmission electron microscopy revealed that the toxin caused early perivascular edema two days after administration, as well as significant damage in astrocytes four days after administration and significant damage in neurons and oligodendrocytes eight days after administration. Interrupted synapses and mast cell extravasation were also found eight days after administration of the toxin. We thus conclude that the chronological order of events observed in the striatum could explain the neurological disorders found eight days after administration of the toxin.

Intracerebroventricular Shiga toxin 2 increases the expression of its receptor globotriaosylceramide and causes dendritic abnormalities.

Neurological damage caused by intoxication with Shiga toxin (Stx) from enterohemorrhagic Escherichia coli is the most unrepairable and untreatable outcome of Hemolytic Uremic Syndrome, and occurs in 30% of affected infants. In this work intracerebroventricular administration of Stx2 in rat brains significantly increased the expression of its receptor globotriaosylceramide (Gb(3)) in neuronal populations from striatum, hippocampus and cortex. Stx2 was immunodetected in neurons that expressed Gb(3) after intracerebroventricular administration of the toxin. Confocal immunofluorescence of microtubule-associated protein 2 showed aberrant dendrites in neurons expressing increased Gb(3). The pro-apoptotic Bax protein was concomitantly immunodetected in neurons and other cell populations from the same described areas including the hypothalamus. Confocal immunofluorescence showed that Gb(3) colocalized also with glial fibrillary acidic protein only in reactive astrocytic processes, and not in vehicle-treated normal ones. Rats showed weight variation and motor deficits as compared to controls. We thus suggest that Stx2 induces the expression of Gb(3) in neurons and triggers neuronal dysfunctions.

Intracerebroventricular administration of Shiga toxin type 2 altered the expression levels of neuronal nitric oxide synthase and glial fibrillary acidic protein in rat brains.

Shiga toxin (Stx) from enterohemorrhagic Escherichia coli (STEC) is the main cause of hemorrhagic colitis which may derive into Hemolytic Uremic Syndrome (HUS) and acute encephalopathy, one of the major risk factors for infant death caused by the toxin. We have previously demonstrated that intracerebroventricular administration of Stx2 causes neuronal death and glial cell damage in rat brains. In the present work, we observed that the intracerebroventricular administration of Stx2 increased the expression of glial fibrillary acidic protein (GFAP) leading to astrogliosis. Confocal microscopy showed reactive astrocytes in contact with Stx2-containing neurons. Immunocolocalization of increased GFAP and Stx2 in astrocytes was also observed. This insult in the brain was correlated with changes in the expression and activity of neuronal nitric oxide synthase (nNOS) by using the NADPH-diaphorase histochemical technique (NADPH-d HT). A significant decrease in NOS/NADPH-d-positive neurons and NOS/NADPH-d activity was observed in cerebral cortex and striatum, whereas an opposite effect was found in the hypothalamic paraventricular nucleus. We concluded that the i.c.v. administration of Stx2 promotes a typical pattern of brain injury showing reactive astrocytes and an alteration in the number and activity of nNOS/NADPH-d. According to the functional state of nNOS/NADPH-d and to brain cell morphology data, it could be inferred that the i.c.v. administration of Stx2 leads to either a neurodegenerative or a neuroprotective mechanism in the affected brain areas. The present animal model resembles the encephalopathy developed in Hemolytic Uremic Syndrome (HUS) patients by STEC intoxication.

Cambios estructurales y ultraestructurales en el cartilago frente a la modulacion del oxido nitrico y la utilizacion de factores de crecimiento / Structural and ultrastructural changes in the cartilage opposite to the nitric oxide modulation and the use of growth factors

La patología del cartílago articular es frecuente en la traumatología, por eso es de nuestro interés dilucidar e investigar la ultraestructura y estructura de la lesión, la reparación de este tejido, con el fin de conseguir avances clínicos en la evolución de estas lesiones frecuentes. Los traumatismos, las lesiones por sobreuso y sobrecarga producen alteraciones en el cartílago, cuyos mecanismos de producción y reparación no han sido aún bien estudiados. El conocimiento de los mismos podría ser de gran aporte para evaluar si la aplicación de factores de crecimiento y el estudio de la modulación del oxido nítrico pueden tener una proyección terapéutica. Existen pocos trabajos que reportan el valor del óxido nítrico y los factores de crecimiento en el desarrollo y la reparación de estas lesiones, y debido al costo económico que provoca la abundancia de estas lesiones a los sistemas de salud, y las secuelas que provocan con el desarrollo tórpido y los tratamientos equívocos que se realizan hoy en día, es importante el estudio y mejora de su evolución para el bienestar del paciente y para disminuir el tiempo de regreso a la actividad laboral que el paciente ejercía.(AU)

Cambios estructurales y ultraestructurales en el cartilago frente a la modulacion del oxido nitrico y la utilizacion de factores de crecimiento / Structural and ultrastructural changes in the cartilage opposite to the nitric oxide modulation and the use of growth factors

La patología del cartílago articular es frecuente en la traumatología, por eso es de nuestro interés dilucidar e investigar la ultraestructura y estructura de la lesión, la reparación de este tejido, con el fin de conseguir avances clínicos en la evolución de estas lesiones frecuentes. Los traumatismos, las lesiones por sobreuso y sobrecarga producen alteraciones en el cartílago, cuyos mecanismos de producción y reparación no han sido aún bien estudiados. El conocimiento de los mismos podría ser de gran aporte para evaluar si la aplicación de factores de crecimiento y el estudio de la modulación del oxido nítrico pueden tener una proyección terapéutica. Existen pocos trabajos que reportan el valor del óxido nítrico y los factores de crecimiento en el desarrollo y la reparación de estas lesiones, y debido al costo económico que provoca la abundancia de estas lesiones a los sistemas de salud, y las secuelas que provocan con el desarrollo tórpido y los tratamientos equívocos que se realizan hoy en día, es importante el estudio y mejora de su evolución para el bienestar del paciente y para disminuir el tiempo de regreso a la actividad laboral que el paciente ejercía

Cambios estructurales y ultraestructurales en el cartilago frente a la modulacion del oxido nitrico y la utilizacion de factores de crecimiento / Structural and ultrastructural changes in the cartilage opposite to the nitric oxide modulation and the use of growth factors

La patología del cartílago articular es frecuente en la traumatología, por eso es de nuestro interés dilucidar e investigar la ultraestructura y estructura de la lesión, la reparación de este tejido, con el fin de conseguir avances clínicos en la evolución de estas lesiones frecuentes. Los traumatismos, las lesiones por sobreuso y sobrecarga producen alteraciones en el cartílago, cuyos mecanismos de producción y reparación no han sido aún bien estudiados. El conocimiento de los mismos podría ser de gran aporte para evaluar si la aplicación de factores de crecimiento y el estudio de la modulación del oxido nítrico pueden tener una proyección terapéutica. Existen pocos trabajos que reportan el valor del óxido nítrico y los factores de crecimiento en el desarrollo y la reparación de estas lesiones, y debido al costo económico que provoca la abundancia de estas lesiones a los sistemas de salud, y las secuelas que provocan con el desarrollo tórpido y los tratamientos equívocos que se realizan hoy en día, es importante el estudio y mejora de su evolución para el bienestar del paciente y para disminuir el tiempo de regreso a la actividad laboral que el paciente ejercía.

Neostriatal cytoskeleton changes following perinatal asphyxia: effect of hypothermia treatment.

Long-term changes of different types of neurofilaments (NF) and glial fibrillar acid protein (GFAP) were studied in neostriatal rat subjected to perinatal asphyxia (PA) under normothermic and hypothermic (15 degrees C) conditions, using immunohistochemistry for light and electron microscopy. Neostriatal neurons of 6-month-old rats that were subjected to 19 and 20 min of PA, showed an increase of NF 200 kDa immunostaining mainly in the axon fascicles in comparison with the control and hypothermia groups. In contrast, no alterations were seen with NF68 and NF160 neurofilament antibodies. Furthermore, the same PA groups showed astroglial cells with enhanced GFAP immunoreactivity, evidencing a typical astroglial reaction with a clear hypertrophy of these cells. A quantitative image analysis confirmed these observations. Hypothermic treated animals did show neither astroglial nor neuronal cytoskeletal changes in comparison to the control group. These findings showed that PA produces chronic cytoskeletal alterations in the neostriatum cells that can be prevented by hypothermia.

Striatal cytomegalic neurons containing nitric oxide are associated with experimental perinatal asphyxia: implication of cold treatment

Neuropathological mechanisms triggered by excitatory aminoacids are Known to involve nitric oxide (NO). Neurons containing NO are histochemically reactive to nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), which labels NO synthase in CNS, Sprague-Dawley male rats subjected to perinatal asphyxia (PA) at 37 degreese Celsius, and PA plus 15 degreese Celsius hypothermia were evaluted when 6 months old by NADPH-d histochemical reaction. Computarized image analysis was used for quantification of stained sections. NADPH-d neurons in striatum from subsevere and severe PA showed a significant increment in soma size and dendritic process length versus control and hypothermic treated rats. Post-ischemic damage reurons are therefore involved in NO changes induced by PA that may be prevented by hypothermia treatment. (AU)

Striatal cytomegalic neurons containing nitric oxide are associated with experimental perinatal asphyxia: implication of cold treatment

Neuropathological mechanisms triggered by excitatory aminoacids are Known to involve nitric oxide (NO). Neurons containing NO are histochemically reactive to nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), which labels NO synthase in CNS, Sprague-Dawley male rats subjected to perinatal asphyxia (PA) at 37 degreese Celsius, and PA plus 15 degreese Celsius hypothermia were evaluted when 6 months old by NADPH-d histochemical reaction. Computarized image analysis was used for quantification of stained sections. NADPH-d neurons in striatum from subsevere and severe PA showed a significant increment in soma size and dendritic process length versus control and hypothermic treated rats. Post-ischemic damage reurons are therefore involved in NO changes induced by PA that may be prevented by hypothermia treatment.