Asunto(s)
Portador Sano/transmisión , Infección Hospitalaria/microbiología , Infección Hospitalaria/transmisión , Personal de Salud , Resistencia a la Meticilina , Infecciones Estafilocócicas/transmisión , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/aislamiento & purificación , Portador Sano/microbiología , Dermatoglifia del ADN , ADN Bacteriano/genética , Brotes de Enfermedades , Electroforesis en Gel de Campo Pulsado , Genotipo , Humanos , Recién Nacido , Unidades de Cuidado Intensivo Neonatal , Italia/epidemiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/clasificaciónRESUMEN
A point-prevalence study of nosocomial infections was conducted in 10 general hospitals in northwestern Italy in June and July 2000. Infection rates were compared by type and site among the different hospitals. Urinary tract infections were most frequent, accounting for 57.8% of 128 nosocomial infections.
Asunto(s)
Infección Hospitalaria/epidemiología , Infección Hospitalaria/prevención & control , Estudios Transversales , Humanos , Incidencia , Italia/epidemiología , Prevalencia , Factores de RiesgoRESUMEN
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
Asunto(s)
Hidrolasas de Éster Carboxílico/metabolismo , Saccharomyces cerevisiae/enzimología , Carboxilesterasa , Electroforesis en Gel de Poliacrilamida/métodos , Fluoresceínas , Dodecil Sulfato de Sodio , Especificidad por SustratoRESUMEN
The A.A. studied the effectiveness of colliriums containing fibronectins aprotinin, and fibronectin associated with aprotinin compared to a placebo using 12 rabbits in which a deep corneal ulcer was induced by alkal. The results showed the same effectiveness of the three collyrium versus the placebo there was clinical recovery in all cases and restoration of corneal trasparency for 13 out of 15 treated eyes. Cellular cultures obtained from primary explants of treated corneal cells, compared with non treated groups, gave the same outcome of the clinical's ones.
