Assessment of Periodontopathogens in Subgingival Biofilm of Banded and Bonded Molars in Early Phase of Fixed Orthodontic Treatment.

To assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. Subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group A comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group B of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface of the same teeth. Subgingival sample collection was performed before bands and tubes application and 4-7 weeks after attachment placement. DNA-strip tehnique was used to assess the presence of eleven putative periodontopathogens at each time point. Fusobacterium nucleatum, Eikenella corrodens and Capnocytophaga spp. were found in a large number of samples, other periodontopathogens were present in a smaller rate. The 4-7 weeks after attachment placement a slight increase of putative species was observed in both groups. The presence of orthodontic tubes and bands influence the accumulation and composition of subgingival microbiota. Higher level of oral hygiene should be achieved before and during orthodontic treatment in order to prevent any side effects on periodontal tissues.

First description of bla(NDM-1), bla(OXA-48), bla(OXA-181) producing Enterobacteriaceae strains in Romania.

We report the first isolation and characterization of several Enterobacteriaceae strains harboring bla(NDM-1), bla(OXA-48) and/or bla(OXA-181) genes in a Romanian emergency teaching hospital. Between January 2010 and September 2012 nine carbapenemase-producing Enterobacteriaceae strains were identified. The bla(NDM-1) gene was present in two Enterobacter cloacae strains, an Escherichia coli and two Klebsiella pneumoniae strains. One of these K. pneumoniae strains also harbored the bla(OXA-181) gene. Three other K. pneumoniae strains and one Serratia marcescens carried bla(OXA-48).

[Antimicrobial use and its correlations with the frequency of carbapenem-resistant Pseudomonas aeruginosa strains in a hospital setting]. / Evaluarea consumului de antibiotice si a efectului acestuia asupra frecventei tulpinilor de Pseudomonas aeruginosa carbapenem rezistente în mediul spitalicesc.

One of the major causes leading to increased antibiotic resistance is excess antimicrobial consumption. We have analysed the correlation between antibiotic use and frequency of carbapenem-resistant Pseudomonas aeruginosa (CRPA) strains in the general intensive care unit of a university hospital Antibiotic use was expressed as number DDD (daily defined doses)/100 occupied hospital bed-days (BD). CRPA incidence rates were determined by number of unique isolates reported to 100,000 BD. The average use of antibiotics between January and August 2008 was 174 DDD/100 BD. The first four most frequently prescribed antibiotics were as follows: first and second generation cephalosporins (47 DDD/100 BD), carbapenemns (29 DDD/100 BD), fluoroquinolones (26 DDD/100 BD) and glycopeptids (20 DDD/100 BD). Average monthly incidence rate of CRPA was 546/100,000 BD (319-773/100,000 BD. CI 95%). There was a correlation between CRPA incidence rate and carbapenem plus fluoroquinolone use (Pearson coefficient of correlation r = 0.7, p < 0.05). Our data showed that the evolution of CRPA incidence rates was related to carbapenem and fluoroquinolone use.

Incidence, antibiotic resistance and clonal relations of MRSA strains isolated from a Romanian university hospital.

Our aim was to estimate the frequency and characteristics ofmethicillin-resistant Staphylococcus aureus (MRSA) strains occurring in a Romanian teaching hospital. We retrospectively studied isolates from infected or colonized patients treated at the intensive care and surgical units during January 2004-December 2005. The antibiotic susceptibility of MRSA strains and the presence of mecA gene were determined. Consecutively occurring strains isolated through a three-month period were typed using pulsed field gel electrophoresis. A total of 423 S. aureus strains were identified, methicillin-resistance was detected in 211 (49.9%) strains. Most of them were multiresistant. One of the MRSA genotypes identified by PFGE was commonly recovered from patients treated in the intensive care unit. According to our results, MRSA strains were frequently isolated pathogens in our hospital and there is an urgent need to enhance infection control efforts.

[Bacterial etiology of the conditions associated with bacteremia/septicemia during the years 2006-2007]. / Etiologia bacteriana a afectiunilor asociate cu bacteriemie/septicemie in perioada 2006-2007.

PURPOSE: Evaluation of the etiology of bacterial diseases associated with bacteremia/ sepsis in the Emergency County Hospital of Tg. Mures during 2006-2007. MATERIALS AND METHODS: Blood samples of 684 patients hospitalized in Emergency County Hospital of Tg. Mures during January 2006 - December 2007 were analyzed. The isolated germs have been identified according to standard methods. The blood culture results were centralized and processed in electronic databases. RESULTS: In 2006 and 2007 the frequency of identified germs from blood cultures remained unchanged: the predominant germs are coagulase-negative staphylococci, followed by enterobacteria (Klebsiella spp prevailing), S. aureus (mostly methicillin-resistant (MRSA)) and other bacteria. CONCLUSIONS: The main bacteria isolated from blood culture are staphylococci and enterobacteria. In medical sections undertaking invasive procedures, the infections with methicillin-resistant staphylococci are common.

[Cumulative antibiogram--means of following local resistance phenomena based on material gathered in the laboratory of microbiology]. / Antibiograma cumulativa--posibilitate de urmarire a fenomenelor de rezistenta locale bazata pe materialul laboratorului de bacteiologie.

The cumulative antibiogram (CA) represents a report which summarizes the susceptibility of commonly isolated microorganisms to usual antibiotics in a defined period of time. We have evaluated the local resistance data during the year 2007 by generating CA from the bacteriology laboratory's database in Mures County Emergency Clinical Hospital for 3 categories of microorganisms: Gram-positive cocci, Gram-negative enteric rods and non-fermenters. A total of 1382 individual antibiotic susceptibility reports were analysed. Gram-positive cocci were susceptible 100% to glycopeptides and oxazolidinones. Fifty percent of S. aureus isolates were methicillin-resistant. More than 90% of S. aureus and MRSA (methicillin-resistant S. aureus) isolates were susceptible to trimethoprim-sulfamethoxazole. Gram-negative enteric rods were 100% susceptible to carbapenems. The susceptibility of Escherichia coli and Klebsiella pneumoniae to broad spectrum cephalosporins was 72% and 26%, respectively. The activity of carbapenems against Acinetobacter spp. and Pseudomonas aeruginosa was 43% and 35%, respectively. Colistin was active in 100% of the isolated nonfermenters. CA is a useful tool for evaluation of local resistance data but careful interpretation of generated data is required in order to avoid under- or overestimation of resistance.

[Intestinal parasitic diseases in children]. / Parazitozele intestinale la copii.

PURPOSE: To compare the incidence of intestinal parasitosis between children with residence in urban and rural areas: to compare the efficacy of parasitologic diagnostic methods. MATERIALS AND METHODS: In our study we included two lots of children. The first lot consisted in 74 children from rural areas from which we collected 44 samples of feces and 55 samples for the "Scotch tape" test. The second lot consisted in 214 children from urban areas from which we collected 44 samples of feces. We examined each sample of feces by three different methods. The study was performed between April to June 2006. RESULTS: The incidence of intestinal parasitosis increases in children from urban areas towards rural areas, and in children between 5 and 10 years. Ascariasis is the most frequent disease in both urban and rural areas. By examination of each fecal sample by three different methods, the number of positive cases increased. CONCLUSIONS: The residence in rural areas and age between 5 to 10 years are risk factors for intestinal parasitosis. The "Scotch tape" test was more efficient in Enterobius vermicularis infection than the methods performed from feces. We recommend using at the same time three diagnostic methods for feces examination to improve the diagnostic sensibility.

[Re-emergent etiologies: Clostridium difficile]. / Etiologii reemergente: Clostridium difficile.

Clostridium difficile-associated disease (CDAD) can range from uncomplicated diarrhea to sepsis and even death. CDAD rates and severity are increasing, possibly due to a new strain. Transmission of C. difficile occurs primarily in health care facilities via the fecal-oral route following transient contamination of the hands of health care workers and patients; contamination of the patient care environment also plays an important role. Education of hospital staff appropriate diagnostic testing, early treatment, and measures for preventing spread of the pathogen could lead to decreasing the morbidity and mortality rates.