[Significance of P504s in distinction between solid pseudopapillary tumor of pancreas and pancreatic neuroendocrine tumors].

OBJECTIVE: To study the significance of P504s in differential diagnosis between solid pseudopapillary tumor of the pancreas (SPTP) and pancreatic neuroendocrine tumors (PanNET). METHODS: Forty-three patients with SPTP and 41 patients with PanNET encountered during the period from 2007 to 2014 were recruited. Immunohistochemical study for vimentin, CD10, chromogranin A, synaptophysin, beta-catenin, CD99 and P504s in SPTP and PanNET was performed. The diagnostic value of P504s in differentiating SPTP from PanNET was analyzed. RESULTS: Immunohistochemical study showed that vimentin, CD10, chromogranin A, synaptophysin and CD99 were expressed both in SPTP and PanNET. All cases of SPTP showed granular cytoplasmic expression of P504s, whereas those of PanNET were negative. CONCLUSION: P504s is a sensitive and useful marker for SPTP and can be used in the distinction between SPTP and PanNET.

[Clinicopathologic analysis of 254 cases of papillary thyroid microcarcinoma].

OBJECTIVE: To evaluate the prognostic impact of tumor size, ultrasonography, central neck lymph node involvement, and age of patients in papillary thyroid microcarcinoma (PTMC). METHODS: Two hundred and fifty-four patients who underwent total thyroidectomy and central neck dissection for PTMC between 2012 and 2014 were included in this retrospective study. Statistical correlation between tumor size and various clinicopathological parameters was assessed by univariate and multivariate analyses. The ultrasound findings were also evaluated. RESULTS: A total of 254 patients (199 females and 55 males) were included in this study. PTMC showed a predilection for female patients, 41-50 years of age (43.3% of all cases, 110/254), and ultrasound showed hypoechoic nodules. Statistically significant correlation was demonstrated between central neck lymph node involvement and the following factors: age and tumor size. A tumor diameter greater than 0.5 mm (67.3% of all cases) most commonly occurred in patients older than 41 years, and was associated with a higher risk of metastatic central neck lymph node involvement (P<0.05). Hashimoto's thyroiditis was noted in the background in 39.4%(100/254) of cases. CONCLUSIONS: Tumor size appears to have a prognostic impact in PTMC, and larger size is more likely to be associated with a higher risk of central neck lymph node involvement. It is controversial whether the etiology of papillary thyroid carcinoma is related to Hashimoto's thyroiditis.

Diagnosis of a granular cell tumour at the abdominal wall using fine needle aspiration cytology and histology: Case report.

This case report describes the use of fine needle aspiration cytology to diagnose a granular cell tumour (GCT) that presented as a painless, palpable mass at the abdominal wall in a 50-year-old woman who had had the mass for ≥1 year prior to presentation. Routine haematoxylin and eosin staining of the cytological smears demonstrated that the specimen was predominantly cellular; it contained both cell clusters and single cells with abundant granular cytoplasm and indistinct cell borders. Cells were fragile and had fairly uniform naked nuclei that were scattered with vacuolated and prominent nucleoli. Background material on the smears included eosinophilic, granular cytoplasmic material and some adipose tissue. Neither mitoses nor necrosis were observed. A cytological diagnosis of a GCT was confirmed by histological examination of a surgical specimen stained with haematoxylin and eosin. This case report found that the GCT had a characteristic cytological appearance and that cells with prominent nucleoli can be present in benign GCTs.

[Tear film function in non-insulin dependent diabetics].

OBJECTIVE: To investigate whether diabetes mellitus is correlated with tear film dysfunction. METHODS: In 100 non-insulin dependent diabetic cases (200 eyes) and 80 non-diabetic controls, the tear break-up time was determined, and the staining with fluorescein sodium on the cornea and conjunctival impression cytology were performed. RESULTS: When compared with the healthy control group, the diabetic group showed decreased tear film break up time (u = 12.60, P < 0.01), increased rate of staining with fluorescein sodium on the cornea (chi(2) = 71.370, P < 0.01) and abnormal conjunctival epithelium. CONCLUSIONS: Non-insulin dependent diabetics are correlated with tear film dysfunction, so they are susceptible persons of the dry eye. The tear film break up time and staining with fluorescein sodium on the cornea should become routine tests of ophthalmology in diabetes mellitus.