Propylene Glycol Potentiates the Inhibitory Action of CTZ Paste on Antibiotic-Resistant Enterococcus faecalis Isolated from the Root Canal: An In Vitro Study.

This study aimed to evaluate if the change of vehicle for CTZ (Chloramphenicol, Tetracycline, zinc oxide, and Eugenol) paste improves the inhibition of Enterococcus faecalis in vitro. The vehicles evaluated alone and mixed with CTZ were Eugenol, propylene glycol (PG), super-oxidized solution (SOS), grapefruit-seed extract (GSE), and 0.9% saline solution as a negative control. A clinical isolate of E. faecalis was morphologically and biochemically characterized, and its antimicrobial susceptibility was tested using 20 antimicrobial agents. Once characterized, the clinical isolate was cultivated to perform the Kirby-Bauer disc diffusion method with paper discs embedded with the different vehicles mixed or used alone, and incubated at 37 °C for 24 h. Data were analyzed using one-way ANOVA, and the means were compared using Tukey test with a significance level of p < 0.05. For vehicles used alone, GSE presented the greatest inhibition showing a statistically significant difference with the rest of the vehicles. When vehicles were mixed with the CTZ paste, PG showed a greater inhibition with a statistically significant difference from the rest of the vehicles. In conclusion, the vehicle used to mix the CTZ paste plays an important role in the inhibition of E. faecalis in vitro; therefore, we consider that this can be an important factor to achieve success in the use of this technique.

Differences in erythrocytes size and shape in prediabetes and diabetes assessed by two microscopy techniques and its association with dietary patterns. Pilot study.

Hemorheology and microcirculation alterations are caused by erythrocyte size and shape (ESS) modifications. People´s diets can alter erythrocyte functions and membrane fluidity by changing cell membrane components. The aim was to identify differences in ESS obtained by scanning electron (SEM) and atomic force microscopy (AFM) in people with prediabetes and type 2 diabetes (T2DM) and assess their relationship with dietary patterns. The study population included 31 participants (14 healthy, 11 with prediabetes, and 6 with T2DM). Dietary intake was assessed by a food frequency questionnaire, and dietary patterns were obtained using principal component analysis. ESS (diameter, height, axial ratio, thickness, and concave depth) were obtained by SEM and AFM. Differences in ESS between groups were observed with SEM (height) and AFM (height, axial ratio, and concave depth). T2DM presented smaller erythrocytes, more elongated and more altered forms. Two dietary patterns were identified: (1) Unhealthy: more refined cereals, high-fat dairy, fast food, sugary beverages, and fewer fruits, fish, seafood, low-fat dairy, and water. (2) Prudent: higher consumption of refined cereals, vegetables, poultry, low-fat dairy and nuts, and lower tortillas, eggs, high-fat dairy, and legumes. Tertile 3 of the Unhealthy dietary pattern had 80% of healthy participants. A difference in diameter and height (0.44 and 0.32 µm, respectively) obtained by SEM was observed when comparing tertile 2 (smaller erythrocytes) versus tertile 3 in the Unhealthy dietary pattern. SEM and AFM are excellent tools to assess ESS. Unhealthy dietary patterns might be associated with altered ESS. HIGHLIGHTS: SEM and AFM are excellent tools to assess erythrocyte size and shape modifications. Two dietary patterns were identified: healthy and prudent. Smaller erythrocytes were observed in the second tertile of the unhealthy pattern.

Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against Serotypes C and K of Streptococcus mutans on Orthodontic Appliances.

Background and Objectives: Streptococcus mutans (S. mutans) is the main microorganism associated with the presence of dental caries and specific serotypes of this bacteria have been related to several systemic diseases limiting general health. In orthodontics, white spot lesions (WSL), represent a great challenge for clinicians due to the great fluctuation of their prevalence and incidence during conventional orthodontic treatments. Although silver nanoparticles (AgNP) have been demonstrated to have great antimicrobial properties in several microorganisms, including S. mutans bacteria, there is no available information about anti adherence and antimicrobial properties of AgNP exposed to two of the most relevant serotypes of S. mutans adhered on orthodontic materials used for conventional therapeutics. The objective of this study was to determine anti-adherence and antimicrobial levels of AgNP against serotypes c and k of S. mutans on conventional orthodontic appliances. Materials and Methods: An AgNP solution was prepared and characterized using dispersion light scattering (DLS) and transmission electron microscopy (TEM). Antimicrobial and anti-adherence activities of AgNP were determined using minimal inhibitory concentrations (MIC) and bacterial adherence testing against serotypes c and k of S. mutans clinically isolated and confirmed by PCR assay. Results: The prepared AgNP had spherical shapes with a good size distribution (29.3 ± 0.7 nm) with negative and well-defined electrical charges (−36.5 ± 5.7 mV). AgNP had good bacterial growth (55.7 ± 19.3 µg/mL for serotype c, and 111.4 ± 38.6 µg/mL for serotype k) and adherence inhibitions for all bacterial strains and orthodontic wires (p < 0.05). The serotype k showed statistically the highest microbial adherence (p < 0.05). The SS wires promoted more bacterial adhesion (149.0 ± 253.6 UFC/mL × 104) than CuNiTi (3.3 ± 6.0 UFC/mL × 104) and NiTi (101.1 ± 108.5 UFC/mL × 104) arches. SEM analysis suggests CuNiTi wires demonstrated better topographical conditions for bacterial adherence while AFM evaluation determined cell wall irregularities in bacterial cells exposed to AgNP. Conclusions: This study suggests the widespread use of AgNP as a potential anti-adherent and antimicrobial agent for the prevention of WSL during conventional orthodontic therapies and, collaterally, other systemic diseases.

Corrigendum to "In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts".

[This corrects the article DOI: 10.1155/2020/1467456.].

Comparison of the retention of conventional dentures after the use of common adhesive brands on poor denture foundations: an in vitro study / Comparación de la retención de dentaduras convencionales con el uso de marcas comerciales de adhesivos en procesos alveolares deficientes: un estudio in vitro

There is no information about the possible impact in denture retention after the use of common denture adhesives (DAs) when poor denture foundations (PDF) are present. Moreover, there is a lack of information about which current formulation provides greater retention and for how long. Twelve models from edentulous patients with different ridge shape and border height were used and complete dentures were manufactured. Four different formulation brands of DAs were tested after 10 minutes and three, six, nine, and 12 hours of DA application using a universal testing machine. The Fittydent® and Fixodent® adhesives had the highest retention at 12 hours. The PDF group increased on average its retention by 400 %. However, the group presented lower retention compared to the good denture foundation group. In conclusion, DAs significantly increased denture retention. The PDF group were the most benefited with the application of DAs. The Fixodent® paste had the highest retention.

No existe información acerca del posible impacto en la retención de dentaduras después del uso de adhesivos dentales comunes (DAs) cuando existen rebordes alveolares deficientes (PDF). Más aun, existe una falta de información acerca de cuál formula actual provee mayor retención y por cuanto tiempo. Doce modelos de pacientes edentulos con diferentes formas y alturas en sus rebordes alveolares fueron usados, y dentaduras completas les fueron realizadas. Cuatro diferentes fórmulas y marcas de DAs fueron evaluadas después de 10 minutos, tres, seis, nueve y 12 horas de que se aplicó el DA usando una maquina universal de pruebas. Los adhesivos Fittydent® y Fixodent® presentaron la retención más alta a las 12 horas. El grupo con PDF incrementó su retención hasta en un 400 %. Sin embargo, el grupo presentó menor retención cuando se comparó con el grupo que posee adecuados procesos alveolares. Los DAs incrementaron significativamente la retención de las dentaduras. El grupo PDF fue el más beneficiado con la aplicación de DAs. La pasta Fixodent® provee la más alta retención.

Dental plaque microbiota of pet owners and their dogs as a shared source and reservoir of antimicrobial resistance genes.

OBJECTIVE: This investigation aimed to detect coincidences in the antimicrobial resistance genes (ARG) profiles between members of a group living in a household and to compare them between other groups in order to establish if an exchange of ARG occurs and if dental plaque microbiota can be considered as a source and reservoir of ARG that can be shared between humans and pets. METHODS: One hundred sixty dental plaque samples were obtained from four groups: Shelter dogs group (n=20), adult pet owners and dogs group (AD group, n=40), adult pet owners, children and dogs group (ACD group, n=60), and adult non-pet owners and children group (AC group, n=40). DNA was obtained, and specific primers with polymerase chain reaction for ARG detection were used. RESULTS: The AD group exhibited the most coincidences in their ARG profiles, 14 (70%) of the 20 profiles coincided in 100% followed by the ACD group with 9 (45%) coincidences. While the AC group was the less coincident group, only 7 (35%) of the 20 profiles coincided. tetM was the most prevalent with 53.1%, followed by tetQ with 52.5% and cfxA with 51.2%, while the less prevalent were tetW with 31.8%, blaTEM-1 with 27.5%, and ermC with 18.7%. CONCLUSION: Dental plaque microbiota can be considered as a source and reservoir of ARG that can be shared between humans and dogs living in a household. The dogs seem to play an important role in the transference of ARG, and the children appear to be the most affected by carrying the most significant number of ARG.

In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts.

Orthodontic brackets release ions that can be reabsorbed in the oral mucosa, potentially causing complications, including cytotoxic effects and mutagenic alterations. The aim was to evaluate the genotoxicity induced by orthodontic appliance alloys in cultures of human gingival fibroblasts by comet assay. Eluates were obtained from the following brackets alloys: EconoLine (SS: stainless steel), MiniMirage (Ni-Ti: nickel-titanium), Nu-Edge (Co-Cr: cobalt-chromium), In-Vu (PC-polycrystals (PC) aluminum oxide), and Monocrystal IZE (monocrystalline (MC) aluminum oxide). Each bracket was sterilized and exposed to a corrosive process for 35 days. The obtained eluates were tested for genotoxicity of human gingival fibroblasts (HGFA) by the alkaline comet assay. All study groups showed genotoxic effects; there was a significant difference (p < 0.0001) among groups. The eluates obtained from Ni-Ti showed a 16-times greater genotoxic effect. There were differences in genotoxicity after comparing the Ni-Ti with SS (p < 0.01) and Co-Cr brackets (p < 0.001). The ceramic was more genotoxic than metallic brackets (SS and Co-Cr), but less than the Ni-Ti. This in vitro model will be useful for further study of early DNA damage caused by brackets and other biomaterials used in the oral cavity before their introduction into the clinical setting.

Altered erythrocyte morphology in Mexican adults with prediabetes and type 2 diabetes mellitus evaluated by scanning electron microscope.

AIM: To evaluate the erythrocyte morphology in people with prediabetes, T2DM and healthy subjects in a Mexican population and its association with biochemical parameters. METHODS: Cross-sectional study consisted of three groups: healthy (HG), people with prediabetes (PG) and with T2DM (DMG). A blood sample was obtained from all participants to assess the erythrocyte morphology, and levels of HbA1c, glucose and lipid profile. Anthropometrical parameters were also evaluated. RESULTS: It was observed that compared with healthy individuals, people with prediabetes presented a significant decrease in the diameter (-0.08 µm, P = 0.014) and height (-0.07 µm, P = 0.004), as well as people with T2DM (-0.33 µm, P < 0.001 in diameter; and -0.36 µm, P < 0.001 in height). Besides, it was found a significant difference in diameter (-0.25 µm, P < 0.001) and height (-0.29 µm, P < 0.001) between the PG and DMG. No significant differences in the axial ratio between groups. Also, HbA1c, glucose, triglycerides, cholesterol, LDL cholesterol, systolic blood pressure, weight, BMI, waist and hip circumference were significantly associated with diameter and height. CONCLUSIONS: Erythrocyte morphological alterations can serve as an indicator of early diagnosis of T2DM and a factor implicated in the course of the clinical condition, so the correction of these alterations could serve as a treatment for prediabetes and T2DM. It is essential to promote constantly checkups of biochemical and anthropometrical parameters, as well as erythrocyte morphological alterations to prevent the onset of prediabetes and T2DM and possible clinical complications.

Interproximal caries and premature tooth loss in primary dentition as risk factors for loss of space in the posterior sector: A cross-sectional study.

Dental caries-a highly prevalent public health problem in preschoolers and school children-is the main cause of premature dental loss during childhood, and this may be related to loss of space in the posterior sector. The objective of this study was to evaluate whether interproximal caries and premature tooth loss (D and/or E) are risk factors for loss of space in the posterior sector.A comparative cross-sectional study (split-mouth type) was performed in schoolchildren (6-8 years old). Seventeen gypsum models were evaluated. These children presented with unilateral loss of a dental organ or interproximal caries (teeth D and/or E) and without such affectations on the other side. Measurements were made with a digital Vernier caliper. The dependent variable was the difference (loss of space, mm) between the control and case sides. The independent variables were type of affectation (interproximal caries or tooth loss), sex, age, arcade, and number of interproximal surfaces affected.The mean age was 6.82 ±â€Š0.44 years and 64.7% were boys. The average space loss was 1.09 ±â€Š0.18 mm (control vs case; P < .0001). A greater loss of space was observed among those who lost a dental organ than those with interproximal caries (P = .0119). A correlation was observed between the variable loss of space and the number of interproximal surfaces affected (r = 0.5712, P = .0166).Interproximal caries and tooth loss were risk factors for loss of space in the posterior segment in this sample of Mexican schoolchildren.

Association of cytokines polymorphisms with chronic peridontitis and rheumatoid arthritis in a Mexican population.

OBJECTIVE: Historically, it has been shown that rheumatoid arthritis (RA) and periodontitis (PE) share pathophysiological similarities and possibly a genetic background. In order to elucidate the genetic background between both diseases, we evaluated the distributions of five SNPs genotypes and all the possible haplotypes composed in subjects with isolated RA, PE, combined diseases and healthy controls. MATERIALS AND METHODS: The study population consisted of 280 Mexican subjects. Genomic DNA was isolated from buccal epithelial cells collected by cheek scrapings and analyzed for the determination of the following SNPs: IL-1α + 4845 (rs17561), IL-1α -889 (rs1800587), IL-1ß + 3954 (rs1143634), IL-1ß -511(rs16944) and TNF-α -308 (rs1800629). RESULTS: After adjustment for age, sex and smoking status, multiple logistic regression analysis revealed a no significant association in the genotype frequencies of TNF-α -308 and IL-1α + 4845 SNPs. Otherwise a significant association was observed in IL-1ß + 3954 and IL-1ß -511 (p < 0.05) while IL-1α -889 was of borderline statistical significance (p = 0.054). Also, we found three negative associated haplotypes with PE: IL-1α + 4845 G/IL-1ß -511 A, IL-1ß + 3954 C/IL-1ß -511 A and interestingly IL-1α -889 C/IL-1ß -511 A also with a positive association with RA. CONCLUSIONS: Some genotypes and haplotypes are associated with the diseases. But it seems that the genetic background of the association between RA and PE needs to be explored deeper.

Association between periodontitis, periodontopathogens and preterm birth: is it real?

PURPOSE: To identify the association between periodontitis and periodontal pathogens with preterm birth despite the strict control of some important confounders, such as infectious processes and criteria for diagnosis of periodontitis during pregnancy. MATERIALS AND METHODS: In this cross-sectional study were included 70 healthy puerperal women between 20 and 35 years without a history of genitourinary infections during pregnancy. Based on the gestational age they were divided into two groups: 45 with term birth (>37 weeks) and 25 with preterm birth (<37 weeks). Previous informed consent, a gynecologic and dental history that included gynecologic and obstetric background, periodontal status applying different authors' criteria of periodontitis diagnosis, presence of periodontopathogens, dental caries and oral hygiene were recorded. RESULTS: There was no association between periodontitis, periodontopathogens and preterm birth. There were no statistical differences applying different authors' criteria diagnosis of periodontitis. Gingivitis status was similar, but probing depth was greater in preterm birth subjects, perhaps they are young women, and this finding could be an early sign of periodontitis. In like manner, the main periodontal bacterial species are not associated with preterm birth, general hygiene and care habits are poorer than term birth subjects. CONCLUSION: We could suggest that preterm birth is a multifactorial condition and the role of periodontitis and the periodontopathogens itself is not sufficient to trigger the preterm birth. There are factors such as infectious processes and diagnostic criteria for periodontitis that could be responsible for controversial results.

[Evaluation of dental treatment impact in a northwestern Mexican school children population]. / Evaluación del impacto del tratamiento odontológico en una población escolar del noroeste de México.

OBJECTIVE: To evaluate through functional teeth index, the impact in schoolchildren in communities with and without dental community support. MATERIAL AND METHODS: A cross-sectional study was carried out in communities with dental care provided by dental clinics of Sinaloa State University. The study sample was 2,083 schoolchildren. We used the criteria proposed by WHO for the index of decayed, missing-teeth (DMFT) for permanent dentition, index for prima- ry teeth (dmft) and the rate of functional teeth (RFT). For bivariate analysis, the nonparametric tests Pearson f and Mann-Whitney U were used. RESULTS: The comparisons of DMFT (0.20) and RFT (13.95) of children with and DMFT (0.43) and RFT (14.82) of children without community dental support showed statistical significant difference (p < 0.05). There was a statistically significant difference in DMFT and RFT between samples with and without community dental support (p < 0.05). The age showed a statistically significant difference between the DMFT and dmft (p < 0.05). Primary dentition required more dental care than permanent dentition. There was access for dental treatment, but it is not used for the population. CONCLUSIONS: The RFT provided precise information than DMFT, mainly due to register the changes of actions focused to recover the teeth function in the oral cavity.

[Predicting risk of caries in schoolchildren from northwestern Mexico: longitudinal study]. / Predicción de riesgo de caries en escolares del noroeste de México: estudio longitudinal.

OBJECTIVE: To analyze the caries risk through the Bratthall's Cariogram (BC) and the frequency of dental caries in a Mexican northwest children population for 12 months period. MATERIAL AND METHODS: A cohort of 583 scholar children between 6 and 10 years old from Sinaloa state was involved for 12 months period (2007-2008). The Bratthall's Cariogram was used to predict caries risk and the. WHO's criteria were used to obtain the caries index. The caries risk association with clinical variables was analyzed by logistic regression analysis and Sperman's Rho rank correlation test was used to evaluate the correlation between DMFT index and BC. RESULTS: The caries risk increased with respect to age (p < 0.05), the CB identified correctly children for high risk (85%) and low risk (65%) caries for a 12 months period. The baseline values of BC showed a positive correlation with DMFT index (0.86 and p = 0.0001); the diagnostic test evaluation showed the following values: positive predictive value of 87%, negative predictive value of 63%, sensitivity of 93% and specificity of 63%. CONCLUSIONS: The caries risk increased with the age in the studied population. The Bratthall's Cariogram is a useful screening test to evaluate the risk for dental caries at individual and population levels.

Clinical evaluation of the accuracy of conventional radiography and apex locators in primary teeth.

PURPOSE: The purpose of this study was to clinically evaluate the accuracy of conventional radiography and 2 apex locators (Root ZX and ProPex) in determining the working length of root canals in primary teeth and to compare the results with scanning electron microscopy (SEM). METHODS: A general medical and oral history was obtained from 1,600 children and 50 children were selected. The working lengths of the primary teeth root canals were determined with conventional radiography and 2 apex locators (Root ZX and ProPex). These results were compared with the ideal standard using SEM. To determine the difference among means of the different methods, the Wilcoxon test was applied. To determine the accuracy of the methods with the ideal standard (SEM), the Lin interclass correlation coefficient (ICC) was used. RESULTS: Sixty-one canals were evaluated and there were no significant difference in the 3 techniques in accuracy of determination of the working length of the canals. The most accurate method of determining the working length of the root canals in primary teeth was the Root ZX (ICC=0.72), followed by the ProPex (ICC=0.70), and the least accurate was conventional radiography (ICC=0.67). CONCLUSION: The apex locators were more accurate than conventional radiography in determining the working length.

Association between caries, obesity and insulin resistance in Mexican adolescents.

AIM: To determine the association among dental caries, obesity and insulin resistance in Mexican adolescents. METHODS: Body mass index, obesity (OB) blood pressure, insulin level, insulin resistance (IR), triglycerides level, serum HDL-cholesterol (cHDL), DMFT index and salivary flow were measured. RESULTS: Anthropometric measures showed a significant statistical difference (p < 0.05). Insulin level was 8.98 for healthy subjects, whereas for OB-IR group was 25.35, there was a statistical significant difference (p < 0.05). Triglycerides level was 88.50 for healthy subjects and 169.40 mg/dL for OB-IR; cHDL was 52.88 for healthy and 41.82 mg/dL for OB-IR group, both showed a statistically significant difference (p < 0.05). Salivary flow was 4.30 for healthy and for OB-IR group was 5.48 ml/min showed a significant statistical difference (p < 0.05). DMFT index was 3.02 for healthy and for OB-IR adolescents was 4.78, showed a significant statistical diference (p < 0.05). The caries component of DMFT index was 1.84 for healthy and was 3.52 for OB-IR adolescents, showed a significant statistical difference (p < 0.05). According to the multivariate analysis, DMFT (OR = 3.10; IC 95% = 0.20-1.02, p = 0.042) and decay (OR = 3.30; IC 95% = 0.19-1.0, p = 0.011) were associated with subjects with OB-IR. CONCLUSION: OB-IR Mexican adolescents showed a positive association with DMFT.

[Epidemiologic study on dental caries and treatment needs in schoolchildren aged six to twelve years from San Luis Potosi]. / Estudio epidemiológico sobre caries dental y necesidades de tratamiento en escolares de 6 a 12 años de edad de San Luis Potosí.

OBJECTIVE: To determine the experience, prevalence and severity of dental caries, as well as the Significant Caries Index (SiC) and the treatment needs (TN) in schoolchildren aged six to twelve years. Equally, to estimate the effect of caries in primary dentition on caries in permanent dentition. MATERIAL AND METHODS: A cross-sectional study was realized in 3865 schoolchildren from San Luis Potosí City, Mexico. Subjects were clinically evaluated by three standardized examiners (kappa > 0.85). For detection of caries was employed the WHO's criteria for primary (dmft) and permanent (DMFT) dentitions. In the statistical analyzes non-parametric test and logistic regression were used. RESULTS: Mean of age was 8.69 +/- 1.79 and 51.3% were women. In the primary dentition: dmft index was 1.88 +/- 2.34 and the caries prevalence (dmft >0) of 56.8%. In account to severity 22.0% and 5.6% have dmft >3 and dmft >6, respectively. In children of six years, the caries prevalence was 56.0% and the SiC of 5.45. In the permanent dentition: DMFT index was 1.11 +/- 2.03 and the caries prevalence (DMFT >0) of 36.8%. In account to severity 11.8% and 2.8% have DMFT >3 and DMFT >6, respectively. In children of twelve years, the DMFT index caries 4.14 +/- 4.15 and the SiC of 9.15. The TN index was of 88.5% for primary dentition and 93.6% for the permanent. In logistic regression we observed associated to caries prevalence in permanent dentition; presence of caries in primary dentition (OR = 6.37; p < 0.001), female sex (OR = 1.33; p < 0.001) and higher age (OR = 1.69; p < 0.001). CONCLUSIONS: Caries prevalence to six years old and DMFT index to twelve years old were highest to values established by WHO. Caries severity in the primary dentition was two times than observed in the permanent dentition. High TN in both dentitions was observed. Presence of caries in primary dentition was a strong risk indicator for to present caries in permanent dentition.