Application of LAMP coupled with NALF for precise detection of mycoplasma pneumoniae.

Mycoplasma pneumoniae (MP),as the most commonly infected respiratory pathogen in community-acquired pneumonia in preschool children,has becoming a prominent factor affecting children's respiratory health.Currently, there is a lack of easy, rapid, and accurate laboratory testing program for MP infection, which causes comparatively difficulty for clinical diagnostic.Here,we utilize loop-mediated isothermal amplification (LAMP) to amplify and characterize the P1 gene of MP, combined with nucleic acid lateral flow (NALF) for fast and visuallized detection of MP.Furthermore, we evaluated and analyzed the sensitivity, specificity and methodological consistency of the method.The results showed that the limit of detection(LoD) of MP-LAMP-NALF assay was down to 100 copys per reaction and there was no cross-reactivity with other pathogens infected the respiratory system. The concordance rate between MP-LAMP-NALF assay with quantitative real-time PCR was 94.3 %,which exhibiting excellent testing performance.We make superior the turnaround time of the MP-LAMP-NALF assay, which takes only about 50 min. In addition, there is no need for precision instruments and no restriction on the laboratory site.Collectively, LAMP-NALF assay targeting the P1 gene for Mycoplasma pneumoniae detection was a easy, precise and visual test which could be widely applied in outpatient and emergency departments or primary hospitals.When further optimized, it could be used as "point-of-care testing" of pathogens or multiple testing for pathogens.

[Study on the Distinguishment of Camouflage Paints Based on Depolarization Characteristics].

As an important part in the modern warfare, camouflage technology plays a critical role in the battlefield, and the results of detection of camouflage target directly affect the results of war. However, there is little paper to detect camouflage paint by depolarization characteristics, so it is of great significance to use the depolarization technology to study the distinguishment of camouflage paints. To address this issue, we studied the mechanism of the scattering of electromagnetic wave, and analysed the relationship between the characteristics of depolarization and mechanism of scattering. Jones Matrix and Mueller Matrix were used to set up the physical model, and the Mueller-Jones Matrix was decomposed with the characteristics of polarization, then the depolarization coefficients(ωd) of the surfaces of the samples was acquired. In this experiment, we measured soil and three kinds of camouflage yellow paints in seven different incident angles to analyze the characteristics of depolazation of the soil and three kinds of camouflage yellow paints' surfaces. Finally, we applied the theory of Fresnel formulas to verify the theoretical model. The results showed that: the depolarization coefficients of the samples' surfaces were related to the scattering, and with the increase of the incident angles, the depolarization coefficients were decline. But in the whole measurement process, the depolarization coefficients of the soil were far above the camouflage paints'. Research indicated that: this article was the first paper which used the depolarization coefficients as an important parameter to identify the camouflage targets, and could identify the camouflage yellow paints in the soil-background accurately and effectively. The processes of the experiments were simpler, and the time was shorter. In modern battlefield, it could identify the camouflage targets quickly and easily, and furnish the precious time for the victory of the war. Therefore, the depolarization technology had a great application value, and the paper had very important significance on the development of camouflage recognition technology.