Sub-millimeter resolution and high-precision φ-OFDR using a complex-domain denoising method.

Phase noise is one of the main obstacles to achieve high spatial resolution, high precision, and large measurement range in φ-OFDR. Here, we proposed a complex-domain denoising method to achieve unwrapping of phase signals. In this method, the wrapped phase was used to construct a complex signal, and then both real and imaginary parts are denoised by using a wavelet packet. The two sets of denoised signals are reconstructed into a complex form, allowing to obtain an unwrapped phase. Additionally, the spatial position correction algorithm addresses the phase decoherence from strain accumulation. Finally, a high numerical aperture optical fiber is used to enhance the Rayleigh scattering intensity by 15 dB. The comprehensive approach yields remarkable results: a sensing resolution of 0.89 mm, a root mean square error of 1.5 µÎµ, and a maximum strain sensing capability of 2050 µÎµ.

Time-optimal trajectory planning based on event-trigger and conditional proportional control.

Trajectory planning is an important issue for manipulators and robots. To get a optimal trajectory, many constraints including actuators specifications, motion range of joints, workspace limitations, etc, and many objectives including the shortest time, the shortest distance, the lowest energy consumption, the minimum oscillations, obstacles-avoiding, etc, should be considered both. In this paper, firstly, the forward kinematics and inverse kinematics of a five axis manipulator are deduced. And, a simple method to choose one appropriate solution from multi solutions of inverse kinematics is proposed. Secondly, an easy-implemented optimization method of trajectory planning is proposed based on seventh order polynomial interpolation, event-trigger mechanism and conditional proportional control (P control). The proposed optimization method can capture the time optimal trajectory, and the actuators specifications including velocity, acceleration of motor can be guaranteed as well. Thirdly, comparative simulations and experiments validate the effectiveness and efficiency of proposed optimization method. The research provides an insight for the application of trajectory optimization on the micro controller with low computing capability and high real-time performance requirement.

Design and simulation analysis of a bionic ostrich robot.

To look for the reason why the biped animal in nature can run with such high speed and to design a bionic biped prototype which can behave the high speed running and jumping ability, this paper takes the fastest bipedal animal in nature: ostrich as the research subject. Firstly, the body structure and motion characteristics of ostrich are investigated. Secondly, a simple mechanical structure of bionic ostrich robot is designed based on the above biological investigated results. The robot is under-actuated with one actuator each leg, with a spring on the tarsometatarsus and a torsion spring on the metatarsophalangeal joint at the foot end. And then the mechanical design of leg structure is optimized. Finally, the high-speed running and jumping running gait is planned, and comparative simulations are implemented with different design requirements among pure rigid and rigid-flexible coupling scheme, which are rigid, only with spring, only with torsion spring, and with spring and torsion spring both, in detail. Simulation results show that the rigid-flexible coupling design scheme and whole body motion coordination can achieve better high speed performance. It provides an insight for the design and control of legged robots.

Label-free and specific detection of soluble programmed death ligand-1 using a localized surface plasmon resonance biosensor based on excessively tilted fiber gratings.

Programmed death ligand-1 (PD-L1) plays an important role in tumor evasion from the host immune system. The level of soluble PD-L1 (sPD-L1) in serum is closely related to tumor aggressiveness and outcomes. This study aimed to propose a localized surface Plasmon resonance (LSPR) biosensor based on excessively tilted fiber grating (ExTFG) coated with large-sized (∼160 nm) gold nanoshells for label-free and specific detection of sPD-L1. The experimental results showed that the limit of detection (LOD) of the immunosensor for sPD-L1 in buffer solutions was ∼1 pg/mL due to the enhanced LSPR effect resulting from the interaction between sPD-L1 molecules and anti-sPD-L1 monoclonal antibodies. The detection of sPD-L1 in complex serum media, such as fetal bovine serum, confirmed that the label-free immunosensor was extremely specific to sPD-L1 and could identify it at a concentration as low as 5 pg/mL. Therefore, it can be potentially applied in clinic for the fast and early diagnosis of cancer.

Immunosensing platform with large detection range using an excessively tilted fiber grating coated with graphene oxide.

We report an immunosensing platform with a large detection range using an excessively tilted fiber grating (ExTFG) coated with graphene oxide (GO). ExTFG was inscribed in standard single-mode fiber; GO film was coated on the fiber surface through hydrogen bond. The effectiveness and uniformity of GO deposited on the ExTFG surface were investigated by field emission scanning electron microscopy and energy spectrum method. Bovine serum albumin (BSA) monoclonal antibodies (MAbs) were used as biometric units to link the GO film through a covalent bond for the specific detection of BSA, so as to evaluate the performances of the proposed biosensor. The whole dynamic immobilization process of BSA MAbs and BSA detection were observed by the spectral evolution of the sensor. Experimental results show that the fabricated GO-coated ExTFG biosensor has a large detection range from 1.5 nM-75 nM and fast response for BSA antigen; the limit of detection is ∼0.88 nM by using an optical spectrum analyzer with a resolution of 0.03 nm, and the dissociation constant KD and the affinity constant KA are calculated to be ∼6.66×10-9 M and ∼1.5×108 M-1, respectively. The proposed GO-coated ExTFG immunosensing platform could lay a foundation for the specific detection of other biomolecules.

Study on spectral and refractive index sensing characteristics of etched excessively tilted fiber gratings.

We investigated the spectral and refractive index (RI) sensing characteristics of the excessively tilted fiber grating (Ex-TFG) with different cladding diameters. The Ex-TFG is inscribed in standard single-mode fiber, and the cladding reduces from 125 µm to around 15 µm by the chemical etching method. Experimental results show that the number of cladding modes decreases, and the spacing of adjacent resonance peaks becomes larger and larger with the reduction of the cladding diameter in the observed wavelength range of 1250-1650 nm. The average RI sensitivity in the index region of 1.33-1.38, the one near 1.33, and the one at around 1.38 of the etched Ex-TFG with a diameter of 15 µm is ∼6.3, ∼5.3, and ∼6.67 fold compared to those of the no-etched Ex-TFG, respectively. Also, the RI sensing performances of the etched Ex-TFG with a diameter smaller than 30 µm are better than those of the Ex-TFG inscribed in SM1500 (4.2 µm/80 µm) fiber in the index region of 1.33. The proposed micronano Ex-TFG has higher RI sensitivity and a more compact structure in biosensing applications, compared to the standard Ex-TFGs and Ex-TFGs inscribed in SM1500 fiber.

Study on vibration sensing performance of an equal strength cantilever beam based on an excessively tilted fiber grating.

An equal strength cantilever beam vibration sensor based on an excessively tilted fiber grating (Ex-TFG) with light intensity demodulation is proposed. The basic principles and sensing characteristics of vibration sensing of an equal strength cantilever beam and Ex-TFG, the combination of which is applied into vibration sensing, are analyzed. An Ex-TFG is attached to the middle axis of an equal strength cantilever beam. As the vibration of a piezoelectric ceramic causes the equal strength cantilever beam to deform, the same and uniform deformation also occurs on the Ex-TFG. Experimental results show that when the thickness of the equal strength cantilever beam is 0.3 mm and the Ex-TFG is at transverse electric (TE) polarization state, the sensing performance is the best, with the maximum acceleration sensitivity reaching 81.065 mv·m-1·s2, and the fast Fourier transform (FFT) main frequency components of the sensing signal accounting for more than 80%. In addition, this sensor is stable in sensing performance, easy in demodulation, simple in structure, high in sensitivity, and easy in manufacture, applicable for the sensing and on-line monitoring of low-frequency vibration signals.

C-terminal antibodies (CTAbs): a simple and broadly applicable approach for the rapid generation of protein-specific antibodies with predefined specificity.

Recent advances in proteomic techniques have resulted in an ever-increasing need to produce antibodies. Here, to address this problem, a technically simple approach of targeting the extreme C-termini of proteins with antibodies (CTAbs) was investigated in proteins secreted by the human pathogen Streptococcus pyogenes. Target proteins were identified by a conventional proteomic approach and CTAbs produced against synthetic five amino acid peptides representing the C-terminus of each target protein. In every case where protein secretion was demonstrated (n = 20), CTAbs were successfully produced and bound specifically to the target protein (100% success rate). The apparent specificity was consistent with the structural heterogeneity of the C-termini of S. pyogenes proteins. The global specificity of CTAb binding was defined using a combinatorial library of synthetic peptides representing structural variants of the original synthetic immunogen. This is a systematic and comprehensive approach for the development of antibodies with defined specificity that can be used in a range of applications.