RESUMEN
Diatom testing is considered a useful method for providing supportive evidence for the diagnosis of drowning in forensic pathology. However, various factors remain controversial for recognizing diatoms, such as being time-consuming and laborious and influencing the consistency of the results. Given the absence of precise and well-defined studies on this subject, this study aimed to determine the relationship between the ability to identify diatoms and researchers with different technical backgrounds. A total of 55 samples from 18 cases, including water, lungs, liver, and kidneys, were treated using the microwave digestion-vacuum filtration-automated scanning electron microscopy (MD-VF-Auto SEM), which was used to compare diatom analyses among three groups of well-trained forensic pathologists (FPs), trained junior employees (JEs), and new trainees (TEs). In addition to achieving similar accuracy of positive findings from drowning cases, counting efficiency was evaluated based on taxonomy records and counting time after viewing more than 5500 diatom images. In contrast to the higher counting efficiency of the JE group than that of the TE group, we observed a statistically significant difference (p < 0.05) in the diatom classification between these two groups. Based on our experiments, an efficient analysis for automatically identifying and classifying diatoms is urgently required.
Asunto(s)
Diatomeas , Ahogamiento , Humanos , Ahogamiento/diagnóstico , Ahogamiento/patología , Microscopía Electrónica de Rastreo , Patologia Forense/métodos , Hígado , Pulmón/patologíaRESUMEN
Two new magnesium phosphite-oxalates were prepared under solvent-free conditions using different amines as structure-directing agents. They feature noncentrosymmetric structures with sql and dia topologies, respectively. The two compounds show moderate second-harmonic generation (SHG) responses under 1064 nm laser irradiation. Theoretical calculations were performed to reveal the origin of their SHG responses.