Exploring organic matter conversion pathway and its effect on nitrogen removal in tidal flow constructed wetlands.

Achieving effective nitrogen removal remains a significant challenge faced by constructed wetlands. Although organic matter is a crucial factor influencing nitrogen removal, little attention has been paid to the impact of organic matter conversion pathways on nitrogen removal in constructed wetlands. Here, we showed that endogenous microorganisms performing carbon internalization could be easily enriched in tidal flow constructed wetlands (TFCWs) under its special rhythmic cycle of anaerobic/aerobic operational mode. Endogenous microorganisms could translate influent carbon sources into intracellular carbons during the anaerobic stage and supply the carbon source for endogenous denitrification after the aerobic stage (rest period). Based on these findings, an innovative combined TFCW and Nitrifying-CW system was developed, and robust total nitrogen (TN) removal (82% on average) was achieved even under carbon source limiting conditions. This performance was a substantial improvement compared to the conventional single bed TFCW with multiple "tides" (corresponding to the multiple contact/rest periods) with TN removal of only 54% on average. Simultaneous nitrification-endogenous denitrification (SNED) was found to be the major nitrogen removal pathway in the proposed system. Compared with classical nitrification-denitrification, simultaneous nitrification-endogenous denitrification brings high nitrogen conversion rates and significantly reduces the demand for oxygen and organic carbon. Furthermore, microbial community analysis indicated that endogenous microorganisms such as Candidatus_Competibacter and Defluviicoccus were successfully enriched, accounting for 50.73% and 3.46% in CW1, and 25.25% and 1.76% in CW2, respectively. Together, these mechanisms allow the proposed system to achieve efficient TN removal.

The effect of novel aquaculture mode on phosphorus sorption-release in pond sediment.

The emergence of aquaculture modes has brought considerable changes to the aquaculture landscape and profoundly influenced environmental processes. However, there is limited research on nutrient cycling in emerging aquaculture modes. This study investigated the characteristics and mechanisms of sediment phosphorus (P) sorption-release in traditional earthen pond culture (TEP) and pond-tank culture mode (PTC), which represents novel aquaculture modes. The results showed that under higher nutrient load, the PTC did not show significant differences in nutrient concentration in water and sediments compared to TEP. Although there are no significant differences in overlying water P concentration between the modes throughout the entire aquaculture period, the trends of its variation over time are different, which significantly affected the P sorption-release characteristics of sediment. Additionally, correlation analysis suggested that calcium-bound P and hot NaOH-extractable organic P may affect the sorption-release characteristics of sediment as active P fractions. The change in redox condition caused by enzyme-mediated organic matter decomposition (such as protein and lipids) is also an important reason for sediment P release. However, the P fractions and organic matter content showed no significant differences between the two modes. Sediment microbial analysis showed that TEP exhibited a significant dominance of inorganic P-solubilizing bacteria, especially Actinobacteria and Bacilli classes. PTC had a higher proportion of organic P-solubilizing bacteria, primarily in the Bacteroidia class. The quantitative results of the key functional gene phoD in organic P decomposition also showed that the abundance in PTC was significantly higher than that in TEP. This suggested that microbial differences may be another reason for differences in P sorption-release behavior. This study revealed the differences in P sorption-release characteristics and mechanisms between the TEP and PTC, which holds positive implications for water quality and pollution management in novel aquaculture modes.

Formation of microorganism-derived dissolved organic nitrogen in intermittent aeration constructed wetland and its stimulating effect on phytoplankton production: Implications for nitrogen mitigation.

To control eutrophication in aquatic ecosystems, enhancing nitrogen removal in the constructed wetland (CW) by upgrading conventional CW to aeration CW is commonplace. However, regulatory efforts have only focused on reducing dissolved inorganic nitrogen (DIN) discharge and disregarding dissolved organic nitrogen (DON). Here, we used experimental mesocosms to investigate the effect of aeration mode on the characteristics of effluent DON in CW. The results showed that intermittent aeration is prone to introduce large amounts of DON and bioavailable DON (ABDON) in the effluents, although it greatly decreases effluent total nitrogen (TN). Analysis of DON fluorescent components and molecular characteristics indicated that suddenly shifting the environment from anoxic condition to aerobic condition in the intermittent aeration CW (IACW) would stimulate microorganisms to release tryptophan and simple aromatic proteins-like substances, which does not occur in the limited continuous aeration CW (CACW). Consequently, the abundance of DON resembling lipids, proteins/amino sugars, and carbohydrates-like molecules in IACW were about 2.1 times higher than that in CACW. Bioassay results showed that Selenastrum capricornutum and Microcystis aeruginosa incubated with effluent from IACW both generate larger phytoplankton biomass than that with CACW effluent, even though IACW effluent contains less TN than its counterpart. Moreover, Microcystis aeruginosa can simultaneously utilize DON and DIN, while Selenastrum capricornutum seem to utilize the DON only when DIN was not available. This result implies that increasing DON discharge may also influence phytoplankton composition and stimulate harmful phytoplankton species. Overall, this study indicates that upgrading CW solely depending on DIN removal level cannot ensure a mitigation of nitrogen-related eutrophication, and more efforts should be paid to curb DON discharge.

Strengthening anoxic glycogen consumption in SNEDPR-CW as a strategy to control PAO-GAO competition under carbon limited condition.

Cooperation between Phosphate and Glycogen Accumulating Organisms (PAOs and GAOs) plays a pivotal role in nutrients removal in simultaneous nitrification endogenous denitrification and phosphorous removal (SNEDPR) systems. Recent findings have expanded the application of SNEDPR from activated sludge system to constructed wetland (CW). However, how to regulate competition between PAOs and GAOs in SNEDPR-based CW is still unclear. Here we showed that, GAOs could easily gain dominance over PAOs in SNEDPR-CW under alternating anaerobic/aerobic (A/O) operational mode. Shortening aerobic hydraulic retention time (HRT) at low oxygen concentration was benefit for simultaneous nitrification endogenous denitrification (SNED) and denitrifying dephosphatation but would reduce the overall phosphorus uptake rate and lead to high phosphorus effluent concentrations. Extended aerobic HRT promoted the proliferation of aerobic GAOs over PAOs, decreasing both enhanced biological phosphorus removal (EBPR) and SNED performance. Surprisingly, by switching the operation of system to alternating anaerobic/aerobic/anoxic (A/O/A) mode, an extraordinary nutrients removal performance with mean nitrogen and phosphorus removal efficiency of 84.57% and 89.37% was achieved under carbon sources limited condition. Stoichiometric analysis demonstrated that adding anoxic stage strengthened the intracellular glycogen oxidization of GAOs for denitrification which compromised its subsequent anaerobic carbon sources uptake and PHA storage and provided sufficient carbon sources for PAOs. Microbial community analysis showed that numerical ratio of GAOs to PAOs decreased from 6.67 under A/O to 4.89 under A/O/A mode, which further indicated strengthening glycogen denitrification of GAOs should be an effective way to regulate microbial competition in order to obtain a desired nutrients removal performance in SNEDPR-CW.