Image processing tools for the validation of CryoEM maps.

The number of maps deposited in public databases (Electron Microscopy Data Bank, EMDB) determined by cryo-electron microscopy has quickly grown in recent years. With this rapid growth, it is critical to guarantee their quality. So far, map validation has primarily focused on the agreement between maps and models. From the image processing perspective, the validation has been mostly restricted to using two half-maps and the measurement of their internal consistency. In this article, we suggest that map validation can be taken much further from the point of view of image processing if 2D classes, particles, angles, coordinates, defoci, and micrographs are also provided. We present a progressive validation scheme that qualifies a result validation status from 0 to 5 and offers three optional qualifiers (A, W, and O) that can be added. The simplest validation state is 0, while the most complete would be 5AWO. This scheme has been implemented in a website https://biocomp.cnb.csic.es/EMValidationService/ to which reconstructed maps and their ESI can be uploaded.

[Analysis of prognostic factors for mortality in bacteremia and fungemia at a university hospital. Experience of 10 years]. / Análisis de factores pronósticos de mortalidad de las bacteriemias y fungemias en un Hospital Universitario. Evolución en 10 años.

OBJECTIVE: The objective of this study was to determine the prognostic factors in relationship to evolution to death of bacteremia-fungemia (BF) episodes occurred in 1986 and to compare them with the results obtained ten years later in 1996. PATIENTS AND METHODS: Prospective study of all BF episodes observed at Hospital Universitario La Princesa, Madrid, during the 1985-1986 and 1996-1997 periods. The same definitions were used for the two study periods. The univariate analysis of results was performed with the chi square test and variables with statistical significance with p < 0.10 in the multivariate analysis with the logistic regression model. RESULTS: A total of 984 episodes were analyzed. There was an increased incidence per 1,000 admissions from 23.58 to 28.44. A change in the relationship of nosocomial acquisition (55.5%-42.6%) to community-acquired episodes (44.1%-57.4%) and an increase in gram-positive organisms (39%-48.6%) compared with gram-negative organisms (53.4%-41.8%) was observed. The organisms recovered most frequently in both periods were Escherichia coli and coagulase-negative Staphylococcus. An overall decrease of mortality rate from 26.2% down to 15.9% (OR: 4.52) was noted. Independent factors with poor prognosis in the first period included age over 60 years (OR: 4.52), underlying disease (OR: 2.79; more than one OR: 6.53), respiratory source (OR: 3.86), DIC (OR: 4.79), hypotension (OR: 3.19); as for the second period, the corresponding independent factors included age > 60 years (OR: 6.48), nosocomial acquisition (OR: 2.62), DIC (OR: 18.7), hypotension (OR: 3.07), and inadequate surgical treatment (OR: 7.61). CONCLUSIONS: In the last ten years the incidence of BF episodes has increased. In contrast, mortality rate has decreased. Factors with poor prognosis, including age > 60, DIC, and hypotension, still persist.

Análisis de factores pronósticos de mortalidad de las bacteriemias y fungemias en un Hospital Universitario. Evolución en 10 años / Analysis of prognostic factors of mortality from bacteremia and fungemia in a University Hospital. A ten-year evolution

Introducción. El objetivo de este estudio ha sido determinar los factores pronósticos, en relación con la evolución a muerte, de las bacteriemias-fungemias (BF) ocurridas en 1986 y comparar con los resultados obtenidos 10 años después, en 1996.Pacientes y métodos. Estudio prospectivo de todos los episodios de BF en el Hospital Universitario de La Princesa de Madrid en los períodos de 1985-1986 y 1996-1997. Se utilizaron las mismas definiciones en los dos períodos de estudio. El análisis univariado de los resultados se realizó con la prueba de Chi cuadrado y las variables con significación estadística con p 60 años (OR: 6,48), adquisición intrahospitalaria (OR: 2,62), CID (OR: 18,7), hipotensión (OR: 3,07) y el tratamiento quirúrgico inadecuado (OR: 7,61). Conclusiones. En los últimos 10 años la incidencia de episodios de BF ha aumentado con disminución de la mortalidad y persistiendo como factores de mal pronóstico la edad > 60 años, CID e hipotensión (AU)