[Correlation of EBV Infection with Expression of TNF-α-Inducing Protein 3 Gene and A20 Protein in Hodgkin's Lmphoma].

OBJECTIVE: To analyze the correlation of EBV infection with expression of TNF-α-inducing protein 3 gene and A20 protein in Hodgkin lmphoma. METHODS: The clinical data and pathological specimens of 65 cases of Hodgkin's lymphoma in our hospital were analyzed retrospectively, and the tissue chips were made for the rich area of the tumor cells. The latent membrane protein 1 encoded by EBV was measured by immunohistochemical staining, and the RNA encoded by EBV was measured by in situ hybridization to analyze the infection state. The gene expression of tumor necrosis factor.α-induced protein 3 was detected by fluorescence in situ hybridization, and the expression of A20 protein encoded by EBV was detected by immunohistochemical staining. The obtained data were processed by SPSS 23.0 version statistical software. RESULTS: The positive rate of latent membrane protein 1 was 26.15% (17/65), the positive rate of EBV encoded RNA was 26.15% (17/65), and the coincidence rate was 100.00%. In 65 patients, A20 protein expression was lost in 18 cases (27.69%), and 14 cases (21.54%) showed homozygous or heterozygous deletion of tumor necrosis factorα protein 3 gene. Only 1 case showed A20 loss combined with homozygous deletion of TNFα inducible protein 3. Correlation analysis showed that EBV infection did not significantly relate with expression loss of A20 protein and the gene deletion of TNF-α inducing protein 3 (P>0.05). CONCLUSION: The expression loss of A20 protein and gene detection of TNFα inducing protein 3 are found in both EBV negative and positive patients with Hodgkin's lymphoma, however the results of immunohistochemical staining and fluorescence in situ hybridization are not complete consistant, the reason may closely relate with the technical factors.

Three new coordination polymers based on bis(4-(4H-1,2,4-triazol-4-yl)phenyl)methane: syntheses, structures, multiresponsive luminescent sensitive detection for antibiotics and pesticides, and antitumor activities.

Three novel coordination polymers (CPs), namely, {[Ag2(L)2(Mo4O13)·(CH3CN)]} n (1), {[Zn(L)(1,4-bdc)2·2(1,4-H2bdc)]} n (2), {[Cd(L)(1,4-bdc)0.5]} n (3) have been synthesized under solvothermal conditions by the reaction of bis(4-(4H-1,2,4-triazol-4-yl)phenyl)methane (L) and varied metal salts. Their structures are determined by single X-ray crystal diffraction, and further characterized by elemental analysis, IR, TGA and PXRD. CP 1 with ammonium molybdate as a secondary ligand displays a 2D network with (2,3,3,3,4)-connected net topology and the point symbol of {4·82}6{4·84·10}2{8}, CP 2 and CP 3 with 1,4-H2bdc as a secondary ligand demonstrate 3D structures with different topologies. CP 2 exhibits high sensibility and low detection limit in the recognition of antibiotics (NZF, NFT and FZD) and pesticide (DCN) identification. CP 1 demonstrates good anti-tumor activity toward the tested glioma cells. The possible luminescent sensitivity and anti-tumor mechanisms are also discussed.

[Clinical Study of Patients with Myelodysplastic Syndrome Transformed into Acute Myeloid Leukemia].

OBJECTIVE: To study the biological characteristics and treatment response of patients with myelodysplastic syndrome (MDS) transformed into acute leukemia(AL). METHODS: Using WHO standard method, the clinical characteristics of patients with MDS into AML were retrospectively analyzed, the related factors influencing the MDS into AML and the treatment response of patients were analyzed. RESULTS: Twenty-six cases (17%) of MDS were transformed into AL among 153 cases of middle and high risk MDS, all of which were AML. The median time of transformation from MDS into AML was 4 months (1-29),and these cases transformed into AML-M2, M4, M5 and M6. In these 26 cases of AML patients, the varying degrees of anemia symptom appeared, 2 cases were with marrow infiltration, 18 cases (69.2%) were with abnormal chromosome karyotype. Compared with karyotype before transformation into AML, the abnormal karyotype in 9 cases had been conversed (new karyotype or disappear once of existing karyotype). Total efficiency of individualized treatment for MDS transformed into AML was 80%. This treatment could improve the patients quality of life. CONCLUSION: Middle and high risk MDS patients are prone to be tranformed into AML. Multiple factors are involved in the transformation of MDS into AML. These patients showed the special biological characteristics and poorer prognosis. Demethylation treatment is helpful to achieve a good near-term curative effect.

3-Benzyl-sulfanyl-1H-1,2,4-triazol-5-amine.

In the title mol-ecule, C(9)H(10)N(4)S, the dihedral angle between the benzene and triazole rings is 81.05 (5)°. In the crystal, N-H⋯N hydrogen bonds link the mol-ecules into infinite zigzag chains along [010].

2-(4-Chloro-phen-yl)acetamide.

In the title compound, C(8)H(8)ClNO, the acetamide group is twisted out the benzene plane with a dihedral angle of 83.08 (1)°. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds, forming layers parallel to the ab plane.

5-(4-Chloro-benz-yl)-1H-tetra-zole.

In the title compound, C(8)H(7)ClN(4), the phenyl and tetra-zole rings are inclined at a dihedral angle of 67.52 (6)°. In the crystal, mol-ecules are linked by an N-H⋯N hydrogen bond into a chain structure along [010]. π-π inter-actions with centroid-centroid distances of 3.526 (1) Šbetween adjacent tetra-zole rings further link the chains, forming a ribbon structure.

Copper-catalyzed N-arylation of amides using (S)-N-methylpyrrolidine-2-carboxylate as the ligand.

(S)-N-methylpyrrolidine-2-carboxylate, a derivative of natural L-proline, was found to be an efficient ligand for the copper-catalyzed Goldberg-type N-arylation of amides with aryl halides under mild conditions. A variety of N-arylamides were synthesized in good to high yields.

Bis(acetato-κO)[1,2-bis-(2-pyridylmeth-oxy)benzene-κN,O,O',N']copper(II) monohydrate.

In the title compound, [Cu(CH(3)COO)(2)(C(18)H(16)N(2)O(2))]·H(2)O, the Cu(II) ion is six-coordinated in a typically Jahn-Teller distorted octa-hedral environment defined by two O and two N atoms from the ligand and two O atoms from acetate anions. A linear chain structure propagating in [010] is built up by inter-molecular O-H⋯O hydrogen bonds involving the uncoordinated water mol-ecules.

Bis(acetato-κO)[1,2-bis-(2-pyridyl-meth-oxy)benzene-κN,O,O',N']copper(II) tetra-hydrate.

In the title compound, [Cu(CH(3)COO)(2)(C(18)H(16)N(2)O(2))]·4H(2)O, the Cu(II) ion is six-coordinated in a Jahn-Teller-distorted octa-hedral geometry environment defined by four O atoms and two N atoms. A chain structure along [100] is built up by inter-molecular O-H⋯O hydrogen bonds involving the uncoordinated water mol-ecules.

2,2'-[(4,6-Dinitro-1,3-phenyl-ene)dioxy]diacetic acid hemihydrate.

The skeletons of both independent mol-ecules of the carboxylic acid hemihydrate, C(10)H(8)N(2)O(10)·0.5H(2)O, are approximately planar [maximum deviations 0.642 (3) and 0.468 (1) Å]. The deviations arise from the twisting of the nitro groups with respect to the aromatic rings [dihedral angles = 3.24 (2) and 27.01 (1), and 7.87 (1) and 16.37 (2)° in the two molecules]. The crystal structure features inter-molecular O-H⋯O hydrogen bonds, which the link the dicarboxylic acid and water mol-ecules into a supra-molecular layer network.

2,2'-(4,6-Dinitro-1,3-phenyl-enedi-oxy)diacetic acid dihydrate.

In the title compound, C(10)H(8)N(2)O(10)·2H(2)O, the skeleton of the dicarboxylic acid mol-ecule is approximately planar, the largest deviation being 0.477 (1) Šfor an O atom of a nitro group; this nitro group is twisted out of the plane of the ring by 24.6 (1)°. Adjacent mol-ecules are linked by O-H⋯O hydrogen bonds, which connect the dicarboxylic acid and water mol-ecules into a three-dimensional supra-molecular network.

Poly[[triaqua-[µ(4)-N-(4-carboxyl-ato-phenyl)-iminodiacetato]sodium(I)zinc(II)] dihydrate].

In the title coordination polymer, {[NaZn(C(11)H(8)NO(6))(H(2)O)(3)]·2H(2)O}(n), the Zn atom is coordinated in a distorted tetra-hedral environment by three carboxyl-ate O atoms from two (4-carboxyl-atophenyl-imino)diacetate ligands and one water mol-ecule; the Na atom is in an distorted octa-hedral coordination environment formed by four carboxyl-ate O atoms from three (4-carboxyl-atophenyl-imino)diacetate ligands and two water mol-ecules. The Zn atoms and Na atoms are linked by (4-carboxyl-atophenyl-imino)diacetate ligands into a three-dimensional framework; the uncoordinated water mol-ecules fill the voids of the skeleton and stabilize it by O-H⋯O hydrogen bonds.

1-Methyl-sulfon-yl-4-nitro-benzene.

In the title compound, C(7)H(7)NO(4)S, the nitro group is twisted by 10.2 (5) ° out of the plane of the benzene ring. Inversion-related mol-ecules are linked by non-classical C-H⋯O hydrogen bonds into dimers featuring an R(2) (2)(10) motif.