RESUMEN
Ras-related protein Rab-10 (RAB10) is involved in tumorigenesis and progression of hepatocellular carcinoma (HCC). Here, we found RAB10, O-GlcNAc transferase (OGT), and O-GlcNAcylation were upregulated in HCC. In addition, RAB10 protein level was prominently positively correlated with the expression of OGT. O-GlcNAcylation modification of RAB10 was then investigated. Here we showed that RAB10 interacts directly with OGT in HCC cell lines, Meanwhile, O-GlcNAcylation enhanced RAB10 protein stability. Furthermore, knockdown of OGT suppressed aggressive behaviors of HCC in vitro and in vivo, while elevated RAB10 reversed these. Taken together, these results indicated that OGT mediated O-GlcNAcylation stabilized RAB10, thus accelerating HCC progression.
Asunto(s)
Carcinoma Hepatocelular , Neoplasias Hepáticas , Proteínas de Unión al GTP rab , Humanos , Carcinogénesis , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patología , Transformación Celular Neoplásica , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patología , Procesamiento Proteico-Postraduccional , Proteínas de Unión al GTP rab/genéticaRESUMEN
A surface plasmon resonance (SPR) sensor comprising photonic crystal fiber (PCF) is designed for magnetic field and temperature dual-parameter sensing. In order to make the SPR detection of magnetic field and temperature effectively, the two open ring channels of the proposed sensor are coated with gold and silver layers and filled with magnetic fluid (MF) and Polydimethylsiloxane (PDMS), respectively. The sensor is analyzed by the finite element method and its mode characteristics, structure parameters and sensing performance are investigated. The analysis reveals when the magnetic field is a range of 40-310 Oe and the temperature is a range of 0-60 °C, the maximum magnetic field sensitivity is 308.3 pm/Oe and temperature sensitivity is 6520 pm/°C. Furthermore, temperature and magnetic field do not crosstalk with each other's SPR peak. Its refractive index sensing performance is also investigated, the maximum sensitivity and FOM of the left channel sensing are 16820 nm/RIU and 1605 RIU-1, that of the right channel sensing are 13320 nm/RIU and 2277 RIU-1. Because of its high sensitivity and special sensing performance, the proposed sensor will have potential application in solving the problems of cross-sensitivity and demodulation due to nonlinear changes in sensitivity of dual-parameter sensing.
RESUMEN
In this paper, a dual-parameter sensor based on surface plasmon resonance (SPR)-photonic crystal fiber (PCF) is proposed, which can be applied in detecting the magnetic field and temperature. In this sensor, two elliptical channels are designed on both sides of the fiber core. The left channel (Ch 1) is coated with gold film and filled with magnetic fluid (MF) to achieve a response to the magnetic field and temperature using SPR. The right channel (Ch 2) is coated with gold film as well as Ta2O5 film to improve the SPR sensing performance. Finally, Ch 2 is filled with polydimethylsiloxane (PDMS) to achieve a response to the temperature. The mode characteristics, structural parameters and sensing performance are investigated by the finite element method. The results show that when the magnetic field is in the range of 50-130 Oe, the magnetic field sensitivities of Ch 1 and Ch 2 are 65 pm Oe-1 and 0 pm Oe-1, respectively. When the temperature is in the range of 17.5-27.5 °C, the temperature sensitivities of Ch 1 and Ch 2 are 520 pm °C-1 and 2360 pm °C-1, respectively. By establishing and demodulating a sensing matrix, the sensor can not only measure the temperature and magnetic field simultaneously but also solve the temperature cross-sensitivity problem. In addition, when the temperature exceeds a certain value, the proposed sensor is expected to achieve dual-parameter sensing without a matrix. The proposed dual-parameter SPR-PCF sensor has a unique structure and excellent sensing performance, which are important for the simultaneous sensing of multiple basic physical parameters.
