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BACKGROUND: Cerebral echinococcosis is relatively rare, and it is important to distinguish cerebral cystic echinococcosis (CCE) from cerebral alveolar echinococcosis (CAE) in terms of pathological diagnosis. We aim to describe the different clinicopathological features among patients with CCE and CAE. METHODS: We collected 27 cases of cerebral echinococcosis which were diagnosed in the Department of Pathology of the First Affiliated Hospital of Xinjiang Medical University from January 1, 2012, to June 30, 2023. We compared the patients' clinical characteristics, MRI features, and pathologic manifestations of CCE and CAE. RESULTS: Among 27 cases of cerebral echinococcosis, 23 cases were CAE and 4 cases were CCE. The clinical manifestations of both CCE and CAE patients mainly included headache (21 patients, 77.78%), limb movement disorders (6 patients, 22.22%), epileptic seizures (4 patients, 14.81%) and visual disturbances (2 patients, 7.41%). The average onset age of CAE cases was 34.96 ± 11.11 years, which was 9.00 ± 7.26 years in CCE cases. All CAE patients presented with multiple involvements in the brain and extracranial organs while all CCE patients observed a solitary lesion in the brain and 3 CCE cases had no extracranial involvement. Lesions of CCE in MRI showed a single isolated circular, which was well demarcated from the surrounding tissues and with no obvious edema around the lesions, whereas CAE lesions presented as multiple intracranial lesions, with blurred edges and edema around the lesions, and multiple small vesicles could be observed in the lesions. The edge of CAE lesions could be enhanced, while CCE lesions have no obvious enhancement. CCE foci were clear cysts with a wall of about 0.1 cm. Microscopically, the walls of the cysts were characterized by an eosinophilic keratin layer, which was flanked on one side by basophilic germinal lamina cells, which were sometimes visible as protocephalic nodes. While the CAE lesion was a nodular structure with a rough and uneven nodule surface, and the cut section was cystic and solid; microscopically, the CAE lesion had areas of coagulative necrosis, and the proto-cephalic nodes were barely visible. Inflammatory cell areas consisting of macrophages, lymphocytes, epithelioid cells, plasma cells, eosinophils, and fibroblasts can be seen around the lesion. Brain tissues in the vicinity of the inflammatory cell areas may show apoptosis, degeneration, necrosis, and cellular edema, while brain tissues a little farther away from the lesion show a normal morphology. CONCLUSIONS: With the low incidence of brain echinococcosis, the diagnosis of echinococcosis and the differential diagnosis of CAE and CCE are challenging for pathologists. Grasping the different clinical pathology characteristics of CAE and CCE is helpful for pathologists to make accurate diagnoses.
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Equinococosis , Humanos , Adulto , Masculino , Femenino , Persona de Mediana Edad , China/epidemiología , Equinococosis/patología , Adulto Joven , Imagen por Resonancia Magnética , Diagnóstico Diferencial , Encefalopatías/parasitología , Encefalopatías/patología , Adolescente , Encéfalo/patología , Encéfalo/parasitologíaRESUMEN
The development of photocatalysts with a wide spectral response and effective carrier separation capability is essential for the green degradation of tetracycline hydrochloride. In this study, a magnetic recyclable Z-scheme ZnO/ZnFe2O4 heterojunction (ZZF) was successfully constructed via the solid phase method, using MIL-88A(Fe)@Zn as the precursor. An appropriate band gap width and Z-scheme charge transfer mechanism provide ZZF with excellent visible light absorption performance, efficient charge separation, and a strong redox ability. Under visible light irradiation, the degradation efficiency of tetracycline hydrochloride for the optimal sample can reach 86.3% within 75 min in deionized water and 92.9% within 60 min in tap water, exhibiting superior stability and reusability after five cycles. Moreover, the catalyst in the water can be conveniently recovered by magnetic force. After visible light irradiation for 70 min, the temperature of the reaction system increased by 21.9 °C. Its degradation constant (35.53 × 10-3 min-1) increased to 5.1 times that at room temperature (6.95 × 10-3 min-1). Using thermal energy enhances the kinetic driving force of the reactants and facilitates carrier migration, meaning that more charge is available for the production of â¢O2- and â¢OH. This study provides a potential candidate for the efficient degradation of tetracycline hydrochloride by combining thermal catalysis with a photocatalytic heterojunction.
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The organic piezoelectric polymer polyvinylidene fluoride (PVDF) has attracted extensive research because of its excellent flexibility and mechanical energy-harvesting properties. Here, the electrospinning technique was taken to fabricate synthesized fiber membranes of a PVDF/cellulose acetate (CA) composite. The obtained PVDF/CA electrospun fiber membranes (EFMs) were employed to prepare a flexible nanogenerator. XRD and FTIR spectroscopy revealed the enhancement of piezoelectric behavior due to an increase in ß-phase in PVDF/CA EFMs compared with cast films. The PVDF/CA fibers (mass ratio of PVDF to CA = 9:1) showed an output voltage of 7.5 V and a short-circuit current of 2.1 µA under mechanical stress of 2 N and frequency of 1 Hz, which were 2.5 and two times greater than those of the pure PVDF fibers, respectively. By charging a 4.7 µF capacitor for 15 min with the voltage generated by the PVDF/CA EFMs, nine LED lamps could be lit. The work provides an effective approach to enhancing the piezoelectric effects of PVDF for low-power electronic loading of macromolecule polymers.
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BACKGROUND: Alveolar echinococcosis (AE) can cause severe liver fibrosis and could be fatal if left untreated. Currently, there are no effective therapeutic options for AE-induced liver fibrosis. In view of the therapeutic potential of adipose-derived stem cells (ADSCs), we investigated whether ADSCs transplantation has the ability to control or reverse fibrosis progression in the liver of Echinococcus multilocularis (E. multilocularis) infected mice. METHODOLOGY/PRINCIPAL FINDINGS: C57BL/6 mice infected with E. multilocularis through portal vein inoculation were intravenously injected with ADSCs isolated from inguinal adipose tissues of 6-8 weeks old mice. Histopathological analysis including heamatoxylin & eosin staining as well as Masson's trichrome staining, and Sirius red staining were performed to access the degree of liver fibrosis. Histopathological examination 30 days after ADSCs transplantation revealed that ADSCs significantly decreased the degree of liver fibrosis in E. multilocularis infected mice by inhibiting the expressions of α-SMA and type 1 collagen deposition. In addition, compared to the non-transplanted group, ADSCs transplantation reduced fibrotic areas in E. multilocularis infected mice. We also found that ADSCs transplantation significantly down-regulated TGF-ß1 and TGF-ßR expressions, while up-regulating Smad7 expression in the TGF-ß/Smad signaling pathway. CONCLUSIONS: ADSCs can alleviate Echinococcus multilocularis infection-induced liver fibrosis by modulating the activity level of the TGF-ß/Smad7 signaling pathway and provide a potential therapeutic approach for E. multilocularis-induced fibrosis.
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Equinococosis/complicaciones , Cirrosis Hepática/terapia , Trasplante de Células Madre Mesenquimatosas , Animales , Colágeno/metabolismo , Equinococosis/terapia , Echinococcus multilocularis/patogenicidad , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/fisiología , Ratones Endogámicos C57BLRESUMEN
BACKGROUND: Alveolar echinococcosis (AE) is a chronic disease caused by the larval stage of Echinococcus multilocularis. Assessing the metabolic activity of AE lesions is critical to evaluate disease progression and survey treatment options. There is an urgent need to identify more rapid, convenient, and non-invasive clinical detection methods to substitute the current techniques. Herein, we evaluated the viability of platelet-derived growth factor-BB (PDGF-BB) as a biomarker for detecting the metabolic activity of AE patients and their correlations with clinicopathological features of AE patients. METHODS: Sera were collected from 28 AE patients and a homogenous cohort of 28 healthy individuals. The concentration of serum PDGF-BB homodimers (sPDGF-BB) was assessed via an enzyme-linked immunosorbent assay (ELISA). Liver tissue samples were obtained from a consecutive series of 28 AE patients who underwent surgical resection. Thereafter, we determined the expression levels of local PDGF-BB and platelet-derived growth factor receptor-ß (PDGFR-ß) through immunohistochemistry (IHC). Correlations of PDGF-BB expression levels with clinicopathological features of AE patients were analyzed using SPSS. RESULTS: The concentrations of sPDGF-BB were significantly lower in AE patients (p < 0.0001), particularly in High Metabolically Active AE patients (HMAE) patients (p < 0.05). The expression levels of PDGF-BB and its receptor were both significantly higher in close liver tissue (CLT) in AE patients (p < 0.0001). We also found that metabolically active AE and sPDGF-BB are significantly negatively correlated (r = -0.624, p = 0.0004). Beside, the local expression levels of PDGF-BB was positively correlated with metabolic activity, PNM stage, and lesion size. Notably, the sPDGF-BB levels were proposed as a potential biomarker for assessing metabolic activity of AE, with 81.0% sensitivity and 85.7% specificity (95% confidence interval, p = 0.003). CONCLUSIONS: Serum levels of PDGF-BB could be a simple, non-invasive, and quick biomarker for assessing the metabolic activity of lesions in AE patients.
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Equinococosis , Echinococcus multilocularis , Animales , Becaplermina , Biomarcadores , Equinococosis/diagnóstico , HumanosRESUMEN
Piezocatalysis, driven by mechanical energy and piezoelectric effect, is of great potential in addressing the environmental issues. In this work, a piezoelectric catalyst was fabricated by growing few-layer MoS2 nanosheets onto CuS, for the piezocatalytic degradation of Rhodamine B (RhB), methylene blue (MB) and hexavalent chromium (Cr (VI)). The excellent removal efficiency of Cr (VI) and RhB can be reached 100% within 180 s, through the piezocatalysis of CuS/MoS2-0.6 driven by mechanical stirring in the dark. Impressively, the piezoelectric current of CuS/MoS2-0.6 is 48 and 35.7 times higher than that of pure CuS and MoS2, respectively. The significantly enhanced piezocatalytic performance can be ascribed to the formation of CuS/MoS2 heterojunction and the piezoelectric field generated by MoS2 nanosheets, which promotes the efficient separation of electrons and holes. This study provides insights into strategies to improve catalytic performance through utilizing mechanical energy and opens a new horizon for environmental remediation.
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BACKGROUND: Alveolar echinococcosis (AE) is a zoonotic disease caused by the larval stage of Echinococcus multilocularis parasitizing in the human liver, causing local pathological changes in the liver and manifesting as hyperplasia, liver fibrosis, atrophy, degeneration, and necrosis. Here, we report a method that can simultaneously isolate hepatocytes and hepatic stellate cells (HSCs) from mice infected with Echinococcus multilocularis. METHODS: A mouse model of AE was established. Hepatocytes and HSCs were isolated from mouse liver using a two-step method combining in situ collagenase perfusion and gradient centrifugation. Expressions of Alb, Desmin, and α-SMA were detected with immunofluorescence to identify the isolated hepatocytes and HSCs. RESULTS: The viability and purity of hepatocytes and HSCs both reached 90% or above. For hepatocytes, clear cell boundaries were observed, and the nuclei were round or oval, with clear nucleoli. There was a homogeneous distribution of the hepatocyte marker Alb in the cytoplasm of hepatocytes. Lipid droplets and Desmin expression were observed in the cytoplasm of freshly isolated HSCs. During the activation of HSCs, the lipid droplets gradually decreased and disappeared with a high expression of α-SMA. CONCLUSION: Hepatocytes and HSCs are simultaneously isolated. This may provide a research tool to investigate the interaction between hepatocytes and HSCs and to investigate the mechanism of Echinococcus multilocularis infection-induced liver pathological changes.
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Separación Celular/métodos , Equinococosis Hepática/patología , Células Estrelladas Hepáticas , Hepatocitos , Hígado/patología , Actinas/metabolismo , Animales , Biomarcadores/metabolismo , Técnicas de Cultivo de Célula , Supervivencia Celular , Desmina/metabolismo , Modelos Animales de Enfermedad , Equinococosis/patología , Echinococcus multilocularis/patogenicidad , Femenino , Células Estrelladas Hepáticas/metabolismo , Células Estrelladas Hepáticas/parasitología , Hepatocitos/metabolismo , Hepatocitos/parasitología , Hígado/parasitología , Ratones Endogámicos BALB CRESUMEN
Thrombosis and infections are the main causes of implant failures (e.g., extracorporeal circuits and indwelling medical devices), which induce significant morbidity and mortality. In this work, an endothelium-mimicking surface is engineered, which combines the nitric oxide (NO)-generating property and anti-fouling function of a healthy endothelium. The released gas signal molecules NO and the glycocalyx matrix macromolecules hyaluronic acid (HA) jointly combine long- and short-distance defense actions against thrombogenicity and biofouling. The biomimetic surface is efficiently fabricated by cografting a NO-generating species (i.e., Tri-tert-butyl 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetate-chelated Cu2+ , DTris@Cu) and the macromolecular HA on an aminated tube surface through one-pot amide condensation chemistry. The active attack (i.e., NO release) and zone defense (i.e., HA tethering) system endow the tubing surface with significant inhibition of platelets, fibrinogen, and bacteria adhesion, finally leading to long-term anti-thrombogenic and anti-fouling properties over 1 month. It is envisioned that this endothelium-mimicking surface engineering strategy will provide a promising solution to address the clinical issues of long-term blood-contacting devices associated with thrombosis and infection.
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Incrustaciones Biológicas , Trombosis , Adhesión Bacteriana , Incrustaciones Biológicas/prevención & control , Plaquetas , Endotelio , Humanos , Propiedades de SuperficieRESUMEN
Forests and wetlands, as two important ecosystems, play a crucial role in reducing the concentration of particulate matters. The main purpose of this study is to reveal the contribution of forest and wetland ecosystems to the reduction of particulate matter. We collected the concentration and meteorological data during the daytime in a forest and a wetland in the Olympic Park in Beijing. The main results are as follows: daily variation in the PM10 and PM2.5 concentration had the similar trend with the lowest value at midday and relatively high values in the morning and at nightfall. In the forest ecosystem, the trend of PM10 concentration at three heights followed the order: 6â m > 10â m > 1.5â m, while that of the PM2.5 followed the order 1.5â m > 10â m > 6â m. In the wetland, PM10 and PM2.5 concentrations at the three heights exhibited the same trend: 10â m > 1.5â m > 6â m. It is a comprehensive impact on concentration which may include vegetation collection rate, meteorological conditions and some kind of human activities. The PM deposition velocity of wetland was higher than that of forest, and showed the highest values in winter both in PM2.5 and PM10. The PM deposition flux in wetland was lower than forest only in autumn, and the value of deposition flux was higher than forest in other seasons. PM concentrations was positively correlated with relative humidity but negatively correlated with temperature and wind velocity.
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Contaminantes Atmosféricos , Humedales , Beijing , China , Ciudades , Ecosistema , Monitoreo del Ambiente , Bosques , Humanos , Material Particulado , Estaciones del AñoRESUMEN
To understand the effect of forests and wetlands on air quality, the PM10 and PM2.5 concentration and meteorological data were collected in the forest and wetland in the Beijing Olympic Forest Park in China from May 2106 to May 2017. The blocking rates of forest and wetland to PMs were calculated under different air quality grades which were divided into six levels base on a technical regulation. And we have got three main conclusions. (1) The diurnal variations of PMs were different in the forest and wetland. It showed a first decrease and then an increase in the forest; the lowest value (PM10 = 40.00â µg/m3, PM2.5 = 5.37â µg/m3) was at approximately 12:00. In the wetlands, the lowest values were recorded at 16:00 (PM10 = 39.63â µg/m3 and PM2.5 = 15.89â µg/m3). (2) Another result showed that the blocking in the forest were significantly higher than that at the wetlands (P < .05), and the blocking effects were much better under lower air quality grades. The blocking rate of PM10 and PM2.5 was the highest when the air quality is excellent in the forest. When it comes to wetland, the highest blocking rate of PM10 appears at good air quality, and the highest of PM2.5 was at serious polluted. (3) In addition, there was negative correlation between PM concentrations and temperature, whereas the correlation between PM concentrations and relative humidity is positive. However, the correlation between blocking and meteorological parameters is weak.
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Contaminantes Atmosféricos , Contaminación del Aire , Beijing , China , Monitoreo del Ambiente , Bosques , Material Particulado , Estaciones del Año , HumedalesRESUMEN
Atmospheric particulate matter (PM) deposition which involves both dry and wet processes is an important means of controlling air pollution. To investigate the characteristics of dry and wet deposition in wetlands, PM concentrations and meteorological conditions were monitored during summer at heights of 1.5 m, 6 m and 10 m above ground level at Cuihu Wetland (Beijing, China) in order to assess the efficiency of PM2.5 (particles with an aerodynamic size of <2.5 µm) and PM10 (particles with an aerodynamic size of <10 µm) removal. The results showed: Daily concentrations of PM, dry deposition velocities and fluxes changed with the same variation trend. The daily average deposition velocity for PM10 (3.19 ± 1.18 cm·s-1) was almost 10 times that of PM2.5 (0.32 ± 0.33 cm·s-1). For PM2.5, the following dry deposition fluxes were recorded: 10 m (0.170 ± 0.463 µg·m-2·s-1) > 6 m (0.007 ± 0.003 µg·m-2·s-1) > 1.5 m (0.005 ± 0.002 µg·m-2·s-1). And the following deposition fluxes for PM10 were recorded: 10 m (2.163 ± 2.941 µg·m-2·s-1) > 1.5 m (1.565 ± 0.872 µg·m-2·s-1) > 6 m (0.987 ± 0.595 µg·m-2·s-1). In the case of wet deposition, the relative deposition fluxes for PM2.5 and PM10 were 1.5 m > 10 m > 6 m, i.e. there was very little difference between the fluxes for PM2.5 (0.688 ± 0.069 µg·m-2·s-1) and for PM10 (0.904 ± 0.103 µg·m-2·s-1). It was also noted that rainfall intensity and PM diameter influenced wet deposition efficiency. Dry deposition (63%) was more tilted towards removing PM10 than was the case for wet deposition (37%). In terms of PM2.5 removal, wet deposition (92%) was found to be more efficient.
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Desecación , Humedad , Material Particulado/química , Estaciones del Año , Agua/química , China , Geografía , Lluvia , Propiedades de Superficie , Factores de TiempoRESUMEN
Urban forests have been shown to be efficient for reducing air pollutants especially for particulate matters (PMs). This study aims to reveal the PM blocking capacity of two common artificial landscape species, Sabina chinensis and Liriodendron chinense and to investigate spatial-temporal heterogeneities by estimating the vegetation collection velocity of coarse (PM10) and fine particles (PM2.5) during different seasons and heights. PM concentration and meteorological data were collected on both leeward and windward sides of trees during the daytime in both summers and winters from 2013 to 2015. Concentration and meteorological monitors were installed at three heights, bottom (1.5 m), middle (3.5 m), and top (5.5 m) of the canopy. The results showed: During daytime, the collection velocity changed and PM2.5 collection velocity was much higher than that of PM10. Furthermore, the maximum collection velocities of L. chinense and S. chinensis occurred at 14:00-16:00 both in summer and winter. Moreover, the collection velocity had a positive correlation with wind speed and temperature. The blocking capacities of L. chinense and S. chinensis varied from season to season, and the concentrations of particulate matter indicate the middle canopy of both species as the most effective part for TSP blocking. Furthermore, these two species are more effective blocking in PM2.5 than PM10. The blocking capacity of S. chinensis is generally better. The vegetation collection is the major process of PM removal near the ground and sedimentation was not taken into consideration near the ground.
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Contaminación del Aire , Bosques , Liriodendron/metabolismo , Beijing , Modelos Teóricos , Material Particulado/análisisRESUMEN
In recent years, the rapid development of industrialization and urbanization has caused serious environmental pollution, especially particulate pollution. As the "Earth's kidneys," wetland plays a significant role in improving the environmental quality and adjusting the climate. To study how wetlands work in this aspect, from the early autumn of 2014 to 2015, we implemented a study to measure the PM concentration and chemical composition at three heights (1.5, 6, and 10 m) during different periods (dry, normal water, and wet periods) in the Cuihu wetland park in Beijing for analyzing the dry deposition flux and the effect of meteorological factors on the concentration. Results indicated that (1) the diurnal variations of the PM2.5 and PM10 concentrations at the three heights were similar in that the highest concentration occurred at night and the lowest occurred at noon, and the daytime concentration was lower than that at night; (2) the PM2.5 and PM10 concentrations also varied between different periods that wet period > normal water period > wet period, and the concentration at different heights during different periods varied. In general, the lowest concentration occurred at 10 m during the dry and normal water periods, and the highest concentration occurred at 1.5 m during the wet period. (3) SO42-, NO3-, and Cl- are the dominant constituents of PM2.5, accounting for 42.22, 12.6, and 21.56%, respectively; (4) the dry depositions of PM2.5 and PM10 at 10 m were higher than those at 6 m, and the deposition during the dry period was higher than those during the wet and normal water periods. In addition, the deposition during the night-time was higher than that during the daytime. Moreover, meteorological factors affected the deposition, the temperature and wind speed being negatively correlated with the deposition flux and the humidity being positively correlated. (5) The PM10 and PM2.5 concentrations were influenced by meteorological factors. The PM2.5 and PM10 concentrations were negatively correlated with temperature and wind speed but positively correlated with relative humidity.