RESUMEN
OBJECTIVE: To evaluate the analgesic effect of Corydalis yanhusuo on trigeminal neuropathic pain.in a rat model. METHOD: Rat model of trigeminal neuralgia pain were established by inducing chronic constriction injury (CCI) of the infraorbital branch of the trigeminal nerve (ION). The effect of Corydalis yanhusuo, a traditional Chinese medicine, in ameliorating the pain was tested. Western blotting was performed to investigate the change of cannabinoid CB1 receptors in the Vc the injury of the infraorbital branch of the trigeminal nerve (ION-CCI). CB1 receptor antagonist AM 251 was applied to observe its effect on the analgesic effect of Yanhusuo. RESULT: Administration of dl-THP (2 mg/kg) intraperitoneally increased the response threshold and the cut-off threshold to the mechanical stimulation in ION-CCI rat models. ION-CCI induced an upregulation of cannabinoid CB1 receptors within the ipsilateral of Vc. The effect of Yanhusuo was antagonized by the application of AM 251. CONCLUSION: The analgesic effect of Yanhusuo involves the participation of CB1 receptors, suggesting that Yanhusuo may offer a useful therapeutic approach for trigeminal neuropathic pain.
Asunto(s)
Analgésicos no Narcóticos/uso terapéutico , Corydalis/química , Medicamentos Herbarios Chinos/uso terapéutico , Receptor Cannabinoide CB1/efectos de los fármacos , Enfermedades del Nervio Trigémino/tratamiento farmacológico , Animales , Masculino , Fitoterapia , Ratas , Ratas Sprague-Dawley , Enfermedades del Nervio Trigémino/etiologíaRESUMEN
PURPOSE: To evaluate the different expression of Cdk5 two activators, p35 and p39 throughout rats Vc development. METHODS: The changes of Cdk5 activity, expression of Cdk5 and p39 in the development in spinal trigeminal subnucleus caudalis(Vc) were studied by Western blotting, immunoprecipitation and kinase assay. Statistical analysis was performed using SPSS11.0 software package. RESULTS: Western blot showed that p39 expression was low at newborn, and highest at the 2-3 week-old rat Vc. In the adult rat Vc, expression declined to the same level as in newborn rat Vc. In contrast, the expression of Cdk5 was constant throughout the development rat Vc. Cdk5 activity in the newborn rat Vc(115.5 Kcpm),which was about 6 times higher than that in normal adult rat Vc(19.0 Kcpm). There was significant difference between each group(P<0.01). CONCLUSIONS: Expression of p35 and p39 is differentially distributed throughout rat Vc. Cdk5/p35 and Cdk5/p39 may play different roles in distinct brain regions during different states of the rat Vc development.