Plasmoblastic lymphoma associated with human immunodeficiency virus.

Plasmoblastic lymphoma (PBL) is a subtype of the diffuse large B-cell lymphoma, typically present as extranodal disease associated with human immune deficiency virus (HIV) infection. PBLs are often the initial manifestation of AIDS. Here we present a case of PBL concerning the oral cavity. A 34-year-old woman presented a tumor in the oral cavity that involved the maxilla and gingiva (confirmed by CT-scan). The gingival biopsy showed a massive infiltration by large lymphoid cells with round, vesicular nuclei, prominent nucleoli, fine chromatin and an significant amount of basophilic cytoplasm which express CD79a, CD138, cytoplasmic lambda light chain and LCA, without staining for CD20, CD38, CD3 and CTK. Serological analysis confirmed HIV positivity. PBLs lack most B-lineage markers, but many express CD79a in at least some of the cells, therefore generate difficulties in differential diagnosis. Overall assessment and correlation of the histopathological and immunohistochemical features with the clinical findings and serology investigation are the most helpful diagnostic tools and can lead to the final diagnosis.

[The incidence of influenzal, para-influenzal and adenoviral antigens in keratoconjunctivitis patients during immunization with NIVGRIP vaccine]. / Incidence des antigènes grippal, paragrippal et adénoviral chez des sujets à kérato-conjonctivite durant l'immunisation avec du vaccin NIVGRIP.

Repeated intranasal administrations of NIVGRIP, influenza inactivated vaccine, to patients with chronic recurrent keratoconjunctivitis, led to a significant reduction of respiratory virus incidence, as revealed by the immunofluorescence test in the conjunctive epithelial cells. The effect is probably due to endogenous interferon stimulation by the vaccine, at the nasopharyngeal level.

[Simultaneous detection by immunofluorescence of several viral infections and Mycoplasma, Chlamydia and Rickettsia burneti infections localized at the level of the conjunctival, nasal and pharyngeal mucosae of patients with recurring chronic keratoconjunctivitis]. / Détection simultanée par l'immunofluorescence de quelques infections à virus, Mycoplasma, Chlamydia et Rickettsia burneti, localisées au niveau des muqueuses conjonctivale, nasale et pharyngienne, chez des malades atteints de kérato-conjonctivite chronique récidivante.

A study was conducted of the viral and inframicrobial populations present in the conjunctival, nasal and pharyngeal mucous membranes by immunofluorescence techniques, using a twelve hyperimmune serum kit, in 120 patients with chronic, relapsing keratoconjunctivitis. Qualitative and quantitative differences were noted in the antigen levels in the three investigated mucous membranes. The number of antigens in the conjunctival and nasal epithelial cells was higher than in the pharyngeal ones: 117 and 113 positive IF tests for the first two against 95 for the third. Incidence of each of the individual antigens was two or three times higher in the conjunctival and nasal mucous membranes, excepting, however, the parainfluenza viruses which were equally frequent in all the three mucous membranes.

[Detection of type 1 and 2 herpetic antigens by immunofluorescence in patients with chronic or recurrent keratoconjunctivitis receiving therapy based on etiology]. / Incidence des antigènes herpétiques des types 1 et 2 décelés en immunofluorescence chez des malades souffrant de kérato-conjonctivite chronique ou récidivante, soumis à une thérapeutique étiologique.

Immunofluorescence (IF) techniques were used to evaluate the therapeutical efficiency of moroxidine hydrochloride against herpes virus (HSV) induced keratoconjunctivitis. In 56 out of the 77 followed-up and treated patients, the IF reactions revealed the presence of both types 1 and 2 of herpes virus, while in 21 only type 1 was found. The treatment with moroxidine hydrochloride led to a reduction of 63% and 92%, respectively, of the incidence of herpes virus type 1 and 2 antigens in the conjunctival cells, coincident with the improvement of clinical symptoms or persistent recovery.

Associated respiratory and herpes virus infections in patients with acute keratitis.

Viral antigens were detected by immunofluorescence in exfoliated conjunctival cells from 100 out of 110 keratitis patients investigated. The overall prevalence of respiratory virus antigens (parainfluenza, influenza, adenovirus) was twice as high as that of herpes antigens. The ratio between respiratory and herpes virus antigens was of 4:1 in the patients with acute respiratory syndromes in the past history, as against 2:1 in those who had had no respiratory illness.

Rapid detection by immunofluorescence of multiple viral infections in patients with keratitis.

The presence of 12 viral (herpes 1 and 2, influenza A(H1N1), A(H3N2) and B, parainfluenza 1, 2, 3, adenovirus 5) and inframicrobial (Chlamydia, mycoplasma, Rickettsia burneti) antigens was investigated by direct or indirect immunofluorescence (IF) reactions in exfoliated conjunctival cells from 110 patients with nonbacterial keratitis and keratoconjunctivitis. A rapid etiological diagnosis could be obtained in 101 (92%) of the cases, parainfluenza and herpes antigens being the most frequently detected. In most of the cases the simultaneous presence of several antigens was made evident. Encouraging results were obtained by the application of a specific treatment based on the diagnosis provided by the IF reaction.