Modified Tunnel Technique Combined with Enamel Matrix Derivative: A Minimally Invasive Treatment for Single or Multiple Class I Recession Defects.

PURPOSE: Gingival recession is a significant problem in the esthetic zone. Connective tissue grafts have been considered the gold standard, but they need a donor site that increases morbidity and discomfort for the patient.The purpose of the present study was to evaluate the clinical results of a modified tunnel technique that consists of replacing the connective tissue graft by enamel matrix derivative, in the treatment of Miller Class I recession defects. MATERIALS AND METHODS: Twenty-six teeth in 14 subjects with Miller Class I recessions were treated using the tunnel procedure plus enamel matrix derivative. The gingival recession, probing depth, clinical attachment level, and the width of the keratinized gingival tissue were recorded. RESULTS: There was a statistically significant reduction in gingival recession (88% of root coverage) and a gain in clinical attachment level (3.1 mm) between baseline and 24 months post-operatively, whereas the change in width of keratinized tissue and in probing depth was not statistically significant. All patients were satisfied with the esthetic appearance and would undergo the same surgery again. CONCLUSION: This technique could be successfully used as an alternative to connective tissue grafts, with the advantage of avoiding the discomfort and morbidity of connective tissue harvesting. CLINICAL SIGNIFICANCE: This modified tunnel technique using enamel matrix derivative potentially represents a clinically and esthetically satisfactory treatment of Miller Class I recession defects.

EBV infection is common in gingival epithelial cells of the periodontium and worsens during chronic periodontitis.

An amplifying role for oral epithelial cells (ECs) in Epstein-Barr Virus (EBV) infection has been postulated to explain oral viral shedding. However, while lytic or latent EBV infections of oro/nasopharyngeal ECs are commonly detected under pathological conditions, detection of EBV-infected ECs in healthy conditions is very rare. In this study, a simple non-surgical tissue sampling procedure was used to investigate EBV infection in the periodontal epithelium that surrounds and attaches teeth to the gingiva. Surprisingly, we observed that the gingival ECs of the periodontium (pECs) are commonly infected with EBV and may serve as an important oral reservoir of latently EBV-infected cells. We also found that the basal level of epithelial EBV-infection is significantly increased in chronic periodontitis, a common inflammatory disease that undermines the integrity of tooth-supporting tissues. Moreover, the level of EBV infection was found to correlate with disease severity. In inflamed tissues, EBV-infected pECs appear to be prone to apoptosis and to produce larger amounts of CCL20, a pivotal inflammatory chemokine that controls tissue infiltration by immune cells. Our discovery that the periodontal epithelium is a major site of latent EBV infection sheds a new light on EBV persistence in healthy carriers and on the role of this ubiquitous virus in periodontitis. Moreover, the identification of this easily accessible site of latent infection may encourage new approaches to investigate and monitor other EBV-associated disorders.

Intraoral metal laser welding: a case report.

The possibility of laser welding of dental prostheses offers great advantages: first, the operator has the possibility of welding on the master model, which decreases the number of passages and thus the possibility of errors and damage, and secondly, the patient attends only a few sessions, and, due to the possibility of fixing the damaged prostheses, there is no need to resort to the technician's laboratory. In a previous study we described the experimental phases of intraoral welding, from the in vitro model on animal jaws with evaluations of the temperature variations during welding through thermal chamber and type K thermocouples. In this study we describe the intraoral welding in vivo on human subjects by using, as in the previous study, a fibre-delivered neodymium:yttrium-aluminum-garnet (Nd:YAG) laser. The in vivo phase allowed a restored prosthesis to be positioned and intraorally welded in the upper central sector with optimal results both in patient's comfort and in aesthetic effects. This first in vivo test confirmed that the use of a laser technique for the intraoral welding of metal prostheses is possible, with no particular problems and risks for the biological structures close to the welding zone.

Osteotome sinus floor elevation using Choukroun's platelet-rich fibrin as grafting material: a 1-year prospective pilot study with microthreaded implants.

OBJECTIVES: The aim of this prospective study was to document, radiographically, changes in the apical bone levels on microthreaded implants placed in subsinus residual bone height, according to a bone-added osteotome sinus floor elevation technique with platelet-rich fibrin (PRF) as grafting material. STUDY DESIGN: Implants were placed using PRF as grafting material in the bone-added osteotome sinus floor elevation (BAOSFE) technique. The survival rate at abutment tightening (6 to 12 weeks of healing) and at 1 year was calculated. The radiographic analysis determined on consecutive radiographs: 1) the mean residual bone height (RBH) under the maxillary sinus at implant placement; and 2) the change in endosinus bone level. Mean and standard deviation were used to assess the endosinus bone changes in the mesial and distal implant sides at 1 year. RESULTS: Between December 2004 and June 2005, 20 consecutive patients were included in the study after bone height measurement by periapical radiographs. Patients included 14 women (70%) and 6 men (30%) with a mean age of 54.8 +/- 11.1 years, range 35 to 73 years; they were treated with 35 Astra Tech implants (Astra Tech Dental Implant System; Astra Tech, Mölndal, Sweden) fulfilling the inclusion criteria. The mean healing time before abutment tightening was 8.3 +/- 1.4 weeks (range 6-12 weeks); by this time, 1 implant was mobile and was removed. At 1 year, all implants were clinically stable and the definitive prostheses were in function, resulting in a survival rate of 97.1%. Nineteen implants (55%) were 11 mm long, 6 implants (17%) were 9 mm, 5 implants (14%) were 8 mm, and 5 implants (14%) were 13 mm. The RBH was measured after implant placement on the radiographs on both implant sides. The mean RBH was 6.5 +/- 1.7 mm: 6.3 +/- 1.3 on the mesial side and 6.7 +/- 2.0 mm on the distal side. Measurements of the changes in the endosinus level on the mesial and distal sides showed that all implants gained endosinus bone. The mean endosinus gain was 3.2 +/- 1.5 mm: 3.5 +/- 1.4 mm on the mesial side and 2.9 +/- 1.6 mm on the distal side. The lowest bone gain was 0.9 mm and 0.1 mm on the mesial and distal sides, respectively. The highest gain was 5.8 mm and 5.2 mm on the mesial and distal sides, respectively. CONCLUSIONS: The BAOSFE procedure with PRF as grafting material can lead to an endosinus bone gain. Despite a limited RBH, a healing period of 2-3 months was found to be sufficient to resist a torque of 25 N.cm applied during abutment tightening. At 1 year, formation of a new recognizable bone structure delimiting the sinus floor was identified radiologically and led to a predictable implant function.

Functional fixation of autotransplanted tooth germs by using bioresorbable membranes.

OBJECTIVE: The purpose of this study was to evaluate the contribution of a bioresorbable membrane placement to the healing of immature teeth after autotransplantation of tooth buds. STUDY DESIGN: Six cases were selected: 2 transplantations of wisdom teeth, 2 for premolar agenesis, 1 for ectopia, and 1 premolar in an incisor position. The crown of each tooth germ and the marginal alveolar bone were covered with a resorbable membrane. RESULTS: The radicular edification was nearly complete, neither ankylosis nor inflammatory resorption was observable, the pulp vitality was preserved, and the periodontal integration was identical to that of other teeth. The membrane ensured contention and stabilization of the transplant, allowed functional stimulation, permitted protection of the coagulum and periodontal cells, and kept the epithelium at a distance. CONCLUSIONS: The transplantations of immature teeth were improved by the use of a resorbable membrane, which caused an optimal functional fixation of the transplanted tooth.