RESUMEN
A novel alphaproteobacterium was isolated from the well water of a thermal bath at Budapest, Hungary. Phylogenetic analysis of the novel strain showed that this bacterium belongs to a distinct lineage among the genus Brevundimonas. Based on the 16S rRNA gene sequence strain FDRGB2bT showed the highest sequence similarity values to Brevundimonas naejangsanensis BIO-TAS2-2T (97.35â%), Brevundimonas viscosa F3T (97.28â%), Brevundimonas vesicularis LMG 2350T (97.27â%), Brevundimonas nasdae GTC 1043T (97.14â%), Brevundimonas vancanneytii LMG 2337T (97.13â%) and Brevundimonas aurantiaca DSM 4731T (97.13â%). The newly isolated bacterium was strictly aerobic, and its optimum growth occurred at 20-30 °C, between pH 8-9 and without NaCl. Movement was with a single polar flagellum, but the cells could also produce stalks. The major isoprenoid quinone of strain FDRGB2bT was Q-10, the major cellular fatty acids were C18â:â1ω7c and C16â:â0, and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and four unknown glycolipids. The characteristic diamino acid in its cell wall is meso-diaminopimelic acid. The G+C content of DNA of the type strain was 69.8 mol%. Strain FDRGB2bT (=DSM 29841T=NCAIM B.02621T) is proposed as the type strain of a novel species with the proposed name Brevundimonas balnearis sp. nov.
Asunto(s)
Caulobacteraceae/clasificación , Filogenia , Microbiología del Agua , Pozos de Agua , Técnicas de Tipificación Bacteriana , Composición de Base , Caulobacteraceae/genética , Caulobacteraceae/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Hungría , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Temperatura , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
18S rDNA sequences of six Naviculaceae species [Amphora montana, Gomphonema parvulum, Eolimna minima (syn. Navicula minima), Eolimna subminuscula (syn. Navicula subminuscula), Navicula veneta and Phaeodactylum tricornutum] were determined in order to assess the monophyly of this important group of diatoms, to date not included in 18S rDNA databases, and also that of the recently described genus Eolimna. Phylogenetic trees were constructed using other known diatom 18S rDNA sequences, and best tree topologies obtained were tested against alternative trees for their reliability. The analyses do not reject the monophyly of Naviculaceae and strongly support the separation of the genus Eolimna from Navicula sensu lato. The two species of Eolimna, however, do not appear to be each other's closest relatives among the species investigated: rather, E. subminuscula shows affinities to A. montana, and E. minima to P. tricornutum. A. montana, a species which it has been proposed should be transferred into a separate taxon from the other five species, was found to have grouped well within them in all analyses.
