A multi-biomarker approach to verify chronic effects on Eisenia andrei earthworms exposed to tailings from one of the world's largest mining disasters.

Mining is of great relevance to the global economy, but its activities are challenging due to socio-environmental impacts. In January 2019, an iron ore tailings dam collapsed in Brumadinho (Minas Gerais, Brazil) releasing 12 × 106 m3 of tailings, causing human losses and devastation around 3.13 × 106 m2 of a watershed. In this context, the present study aimed to investigate the potential toxic effects of tailings from the collapsed dam using earthworms Eisenia andrei as a model organism for terrestrial environments. An extensive set of tests was performed, including behavioral (avoidance), acute (mortality and biomass) and chronic tests, such as biomass, reproduction and cytotoxicity (viability and cell density and change in coelomocyte pattern). The physical-chemical characterization revealed a higher density of the tailings in relation to the control soil, which can result in physical changes, such as soil compaction and surface sealing. Aluminum, Ca, Fe, Hg, Mg, Mn, K, Na and P registered higher concentrations in the tailings compared to the control soil, while Total Nitrogen, Total Organic Carbon and Organic Matter were higher in the natural soil. Based on the avoidance test, an EC50 of 27.18 ± 2.83% was estimated. No lethality was observed in the acute exposure, nor variations in biomass in the acute and chronic assays. However, there was a tendency to reduce the number of juveniles in relation to cocoons in the proportions of 3125; 12.5 and 25%. Significant changes in viability, cell density and pattern of amebocytes and eleocytes were observed up to the 35th day of exposure. A multi-biomarker approach (Integrated Biological Response version 2) indicated concentration-dependent effects and attenuation of cellular changes over time. These are the first results of chronic effects on earthworms exposed to tailings from the B1 dam. Despite being conclusive, we highlight the possible heterogeneity of the tailings and the necessary care in extrapolating the results.

Brominated flame retardants and natural organobrominated compounds in a vulnerable delphinid species along the Brazilian coast.

Guiana dolphins, Sotalia guianensis, are vulnerable to extinction along their distribution on the Brazilian coast and assessing chemical pollution is of utmost importance for their conservation. For this study, 51 carcasses of Guiana dolphins were sampled across the Brazilian coast to investigate legacy and emerging brominated flame retardants (BFRs) as well as the naturally-produced MeO-BDEs. PBDEs and MeO-BDEs were detected in all samples analyzed, whereas emerging BFRs were detected in 16 % of the samples, all in Rio de Janeiro state. PBDE concentrations varied between 2.24 and 799 ng.g-1 lipid weight (lw), emerging BFRs between 0.12 and 1.51 ng.g-1 lw and MeO-BDEs between 3.82 and 10,247 ng.g-1 lw. Concentrations of legacy and emerging BFRs and natural compounds varied considerably according to the sampling site and reflected both the local anthropogenic impact of the region and the diversity/mass of biosynthesizers. The PBDE concentrations are lower than what was found for delphinids in the Northern Hemisphere around the same sampling period and most sampling sites presented mean concentrations lower than the limits for endocrine disruption known to date for marine mammals of 460 ng.g-1 lw, except for sampled from Santa Catarina state, in Southern Brazil. Conversely, MeO-BDE concentrations are higher than those of the Northern Hemisphere, particularly close to the Abrolhos Bans and Royal Charlotte formation, that are hotspots for biodiversity. Despite the elevated concentrations reported for this group, there is not much information regarding the effects of such elevated concentrations for these marine mammals. The distinct patterns observed along the Brazilian coast show that organobrominated compounds can be used to identify the ecological segregation of delphinids and that conservation actions should be planned considering the local threats.

Trophic dynamics of methylmercury and trace elements in a remote Amazonian Lake.

Information on pollutant trophodynamics can be crucial for public health, as contaminated food consumption may lead to deleterious effects. This study was performed in Puruzinho Lake, a remote body of water in the Brazilian Amazon from which a riparian human population obtains an important part of its animal protein intake. Samples from 92 individuals, comprising 13 species and four trophic guilds (iliophagous, planktivorous, omnivorous, and piscivorous fish) were analysed for the determination of trace elements (Fe, Cr, Mn, Ni, Zn, Ca, Sr, Cd, Sn, Tl and Pb) and methylmercury concentrations. Samples from the same individuals had already been analysed for stable isotope (SI) measurements (δ13C and δ15N) in a previous investigation and the SI data have been statistically treated with those generated in this study for the evaluation of trophic dynamics of contaminants. Methylmercury was the only analyte that biomagnified, presenting TMF values of 4.65 and 4.55 for total and resident ichthyofauna, respectively. Trace elements presented either trophic dilution or independence from the trophic position, constituting a behaviour that was coherent with that found in the scientific literature. The similarity between Ni behaviour through the trophic web to that of essential elements contributes to the discussion on the essentiality of this metal to fish. Considering the Non-cancer Risk Assessment, the calculated Target Hazard Quotient (THQ) values were higher than 1.0 for all analysed individuals for methylmercury, as well as for only one individual for nickel. No other analyte rendered THQ values higher than 1.0.

First assessment of atmospheric pollution by trace elements and particulate matter after a severe collapse of a tailings dam, Minas Gerais, Brazil: An insight into biomonitoring with Tillandsia usneoides and a public health dataset.

In this study, samples of bromeliad Tillandsia usneoides (n = 70) were transplanted and exposed for 15 and 45 days in 35 outdoor residential areas in Brumadinho (Minas Gerais state, Brazil) after one of the most severe mining dam collapses in the world. Trace elements aluminum (Al), arsenic (As), chromium (Cr), copper (Cu), iron (Fe), mercury (Hg), manganese (Mn), nickel (Ni) and zinc (Zn) were quantified by atomic absorption spectrometry. Scanning electron microscope generated surface images of T. usneoides fragments and particulate matter (PM2.5, PM10 and PM > 10). Aluminum, Fe and Mn stood out from the other elements reflecting the regional geological background. Median concentrations in mg kg-1 increased (p < 0.05) between 15 and 45 days for Cr (0.75), Cu (1.23), Fe (474) and Mn (38.1), while Hg (0.18) was higher at 15 days. The exposed-to-control ratio revealed that As and Hg increased 18.1 and 9.4-fold, respectively, not showing a pattern associated only with the most impacted sites. The PM analysis points to a possible influence of the prevailing west wind on the increase of total particles, PM2.5 and PM10 in transplant sites located to the east. Brazilian public health dataset revealed increase in cases of some cardiovascular and respiratory diseases/symptoms in Brumadinho in the year of the dam collapse (1.38 cases per 1000 inhabitants), while Belo Horizonte capital and its metropolitan region recorded 0.97 and 0.37 cases, respectively. Although many studies have been carried out to assess the consequences of the tailings dam failure, until now atmospheric pollution had not yet been evaluated. Furthermore, based on our exploratory analysis of human health dataset, epidemiological studies are required to verify possible risk factors associated with the increase in hospital admissions in the study area.

Evaluating total mercury and methylmercury biomagnification using stable isotopes of carbon and nitrogen in fish from the Madeira River basin, Brazilian Amazon.

The isotopic signatures of carbon in primary consumer fish species in the Madeira River basin (Western Amazon) follow a pattern, with basal species feeding mainly on C3 (e.g., vascular plants and phytoplankton). In this study with 196 specimens of 19 fish species and six trophic guilds, significant differences were found between the two main groups of primary consumers (herbivores and detritivores) in the Madeira River basin. The mercury and δ15N data indicated that the fish specimens collected in Puruzinho and Cuniã Lakes provided greater trophic magnification factors (TMFs) for this metal than those found in the corresponding rivers. In relation to total mercury (0.09-2.07 µg g-1) and methylmercury (0.05-1.91 µg g-1) concentrations, the piscivorous/carnivorous species exceeded the values recommended by the World Health Organization, with a [MeHg:THg] ratio of about 80%. Preventive and educational practices should be adopted to reduce the possible toxic effects of Hg in the riverside population, for whom fish constitutes the main protein source. Also, biomonitoring of the fish species in the Madeira River should be conducted on an ongoing basis to detect possible alterations in mercury concentrations.

Mercury methylation upon coastal sediment resuspension: a worst-case approach under dark conditions.

Mercury behavior upon resuspension of sediments from two impacted areas of Guanabara Bay was evaluated to assess worst-case methylmercury (MeHg) responses, under dark experimental conditions to prevent demethylation by photolysis. Study areas include the Rio de Janeiro Harbor (RJH) and the chlor-alkali plant-affected Meriti River (MR) estuary. Total mercury (THg) and MeHg concentrations were determined along 24-h experiments of sediment resuspension in the bay water in dark conditions. Fine-grained Meriti River (MR) estuary sediments had 8 times higher MeHg initial concentrations than sandy Rio de Janeiro Harbor (RJH) sediments (3.4 ± 0.29 vs. 0.41 ± 0.1 ng g-1, respectively). Though THg contents were uncorrelated with resuspension time, statistically significant correlations of MeHg (rs = 0.78) and %MeHg in relation to THg (rs = 0.86) with resuspension time were observed for RJH sediments, indicating net methylation only for this study site. These positive correlation trends correspond to a 2.8 times MeHg concentration increase (ΔMeHg = 0.75 ng g-1) and 4.4 times increase in %MeHg (Δ%MeHg = 1.0%), after 24 h of resuspension. This suggests that assessments of factors affecting the MeHg spatial-temporal variability and associated toxicity risks can be limited in some sites if concentration changes due to sediment resuspension-redeposition processes are not considered. Therefore, the inclusion of MeHg evaluation before and after sediment resuspension events is recommendable for the improvement of dredging licensing and monitoring activities.

Flood-pulse and trophic position modulate mercury concentrations in fishes from an Amazon floodplain lake.

Mercury (Hg) bioaccumulation can be influenced by biological characteristics (e.g., trophic position) and environmental changes (e.g., seasonal flood-pulse), as it can create site conditions conducive to methylation and alter the availability of food to biota. This study aimed to relate total mercury (THg) and methylmercury (MeHg) concentrations in fishes of an Amazonian lake (Catalão Lake) to their dietary changes during different hydrological seasons (rising-, high-, falling-, and low-water). Levels of THg, MeHg, and stable isotopes of nitrogen and carbon (δ15N and δ13C) were determined in the muscles of six fish species: Rhytiodus microlepis, Hemiodus sp., Triportheus albus, Anodus elongatus, Pellona flavipinnis and Acestrorhynchus falcirostris. Triportheusalbus, an omnivorous fish, showed a significant relationship between Hg concentrations and δ13C signatures (negative regression; THg: p = 0.0001 and MeHg: p = 0.0002), as well as between Hg and δ15N signatures (positive regression; THg: p = 0.003 and MeHg: p = 0.021), indicating that changes in feeding habits influenced the Hg concentrations in this species. The herbivorous R. microlepis presented the highest MeHg concentrations during the high-water season, reflecting Hg methylation in its diet (macrophyte roots) during flooded season. The piscivorous A. falcirostris had the lowest THg concentrations during this season because of lower prey availability. Slopes of the regressions between the Hg concentrations and δ15N values followed the sequence of rising- < high- < falling-water seasons (THg: 0.06 < 0.11< 0.15; MeHg: 0.07 < 0.10 < 0.17), indicating that the difference between the THg and MeHg concentrations observed for lower- and higher-trophic-level fish increased during the falling-water season, resulting in trophic biomagnification. The present study shows that feeding habit and the flood-pulse influence mercury concentrations in the muscle of fishes from Catalão Lake.

Trophic position, altitudinal distribution, and water dependence as determining factors for mercury concentrations in tropical montane anurans.

Mercury (Hg) is a widespread and toxic contaminant with potential for long-range atmospheric transport. Previous work has shown that temperate and subtropical montane ecosystems have great potential for deposition of this element. However, little information exists regarding Hg dynamics in tropical mountains. In present study, we evaluated the influence of altitudinal distribution, size, trophic position, and degree of water dependence on Hg concentration in amphibians. For this purpose, we determined the mercury concentration in topsoil and amphibian samples collected at 32 points distributed between 327 and 2181 m above sea level in Serra dos Órgãos, a mountainous complex located in southeastern Brazil. We analyzed the concentration of mercury in whole body samples of 200 individuals of 30 amphibian species. Trophic position of the specimens was estimated by nitrogen stable isotope (δ15N) composition in muscle tissues. We observed a positive relationship between elevation and Hg concentration in topsoil samples from rainforest sites. However, in samples from nebular forest and campos de altitude (highland grasslands) sites, the concentration of Hg was considered lower than expected by the trend in rainforest points, indicating that the vegetation structure plays an important role in the deposition of atmospheric mercury. Mercury concentration in amphibians varies according to the functional characteristics of the species and the environment in which the individual is inserted. Elevation, trophic level and water dependence explained at least some degree of variation in Hg concentration in amphibian tissues. Thus, this community-level analysis suggests that mountainous areas in the tropical region, as recorded for temperate and subtropical mountains, act as regional convergence and deposition sites for atmospheric mercury.

Antioxidant system alterations and biological health status of earthworms following long-term exposure to antibiotic-contaminated poultry litter.

Poultry litter is widely applied as a fertilizer even though it is one of the main antibiotic sources to agricultural soils. Long-term sublethal effects (56 days) on the antioxidant system of Eisenia andrei earthworms following exposure to fluoroquinolone-contaminated poultry litter (enrofloxacin + ciprofloxacin) at 5.0, 10, and 20 g kg-1 were evaluated. The following biomarkers were assessed: superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH), and a lipid peroxidation (LPO) proxy. Significant CAT and SOD increases, and a moderate positive correlation (ρ = 0.67, p < 0.05) between these enzymes was observed. Glutathione-S-transferase levels increased significantly at 10 g kg-1, while GSH exhibited a dose-dependent response at 5.0 mg kg-1 (4-106%), 10 mg kg-1 (28-330 %), and 20 mg kg-1 (45-472%). LPO levels exhibited a decreasing trend with increasing poultry litter concentrations of 8-170% (5.0 g kg-1), 7-104% (10 mg kg-1), and 3-6% (20 mg kg-1). A principal component analysis (PCA) highlighted increased SOD and CAT activities, possibly due to increased reactive oxygen species (ROS) concentrations. Biological health status assessments based on the biomarker response index indicate major alterations in the first month of exposure and becoming moderate in the second month. These findings indicate an antioxidant system attenuation trend. It is possible, however, that successive poultry litter applications may reduce the long-term recovery capacity of the evaluated biomarkers.

Trophodynamics of trace elements in marine organisms from cold and remote regions of southern hemisphere.

Trace metals bioaccumulate in aquatic organisms and some of them biomagnify through food webs, posing a threat to the organisms or their human consumers. Although the trophodynamics of many trace metals is well known in the northern hemisphere, much less is known about metals in aquatic food webs from cold and remote coastal zones of the southern hemisphere. To fill this gap, we investigated the trophodynamics of Al, Co, Cr, Li, Mo, Ni, Sr, and V, which were measured in marine macroinvertebrates and fishes from inshore and offshore locations in each of the Chilean Patagonia and the Antarctic Peninsula area. In Patagonia, there was biodilution of these metals across the whole food web, while biomagnification of Li and Ni was significantly found across the lower food web at the offshore site. In Antarctica, significant biodilution of Al, Li, Ni, Mo, Sr and V occurred through the whole food web for the inshore site, but no tendency (biodilution or biomagnification) was found (p > 0.05) across the organisms at lower trophic levels for the offshore site. Our data suggest that the geographic location and species influences the trophodynamics of these trace elements and expand our understanding of metal fate in remote locations of the southern hemisphere.

A review on pesticides in flower production: A push to reduce human exposure and environmental contamination.

In several countries, flower import regulations are restricted to food security, by establishing maximum residue limits (MRL) for pesticides in flower-based food products and biosafety, in order to limit the circulation of vectors, pests and exotic species across borders. In this context, the lack of limits on pesticides in flower-products for ornamental purposes can influence the pesticide overuse in production areas, as well as the transfer of contaminated products between countries. Therefore, the purpose of this review was to discuss possible adverse effects on human and environmental health of pesticides used in floriculture, evaluating regulations on the use of these pesticides in the main importing and flower-producing countries. This review included 92 documents. The use of 201 compounds was identified by interviews and analytical measurements. Among them, 93 are banned by the European Union (EU), although 46.3 % of these compounds have been identified in samples from European countries. Latin American countries have a large number of scientific publications on pesticides in flower production (n = 51), while the EU and China have less studies (n = 24) and the United States and Japan have no studies. Regarding adverse health effects, poorer neurobehavioral development, reproductive disorders, congenital malformations and genotoxicity have been reported for residents of flower production areas and workers throughout the flower production cycle. Studies including water samples show overuse of pesticides, while environmental impacts are related to water and air contamination, soil degradation and adverse effects on the reproduction and development of non-target organisms. This review points out that the absence of MRL for non-edible flowers can be crucial for the trade of contaminated products across borders, including pesticides banned in importing countries. Furthermore, setting limits on flowers could reduce the use of pesticides in producing countries.

Mercury Concentrations in Four Marine Fishery Resources from Rio de Janeiro Coast, SW Atlantic, and Potential Human Health Risk Via Fish Consumption.

Mercury (Hg) contamination has caused ecological and socioeconomic problems worldwide. One of the main Hg contamination routes by the human populations is through fish ingestion. Herein, we analyzed the total Hg concentrations (THg) in highly consumed marine fish species: Brazilian flathead Percophis brasiliensis, Atlantic bigeye Priacanthus arenatus, Stripped weakfish Cynoscion guatucupa, and Jamaica weakfish C. jamaicensis. The specimens were collected from fishing landings off the Rio de Janeiro, SW Atlantic. Additionally, we calculate the estimated weekly intakes (EWI) and the maximum amount of fish (MAF) that can be safely ingested, both based on the provisional tolerable weekly intake (PTWI). The highest THg concentrations were recorded in P. brasiliensis with a mean of 340.59 µg/kg (dry weight) and the lowest in P. arenatus (115.75 µg/kg). THg concentrations were positively related to the δ15N ratios indicating an increase in contamination with trophic level. All samples showed THg concentrations below the established limits by Brazilian and international regulation. Hg intake via human fish consumption does not exceed the PTWI. The EWI varied from 1.41% (P. arenatus size class I) to 11.52% (P. brasiliensis class II) of the PTWI, while the estimated EWI for "fish consumers" varied between 8.35 and 68.07% PTWI. The MAF estimated for an adult (70 kg) could safely consume between 1277.85 and 7075.50 g/week. This study is an important baseline for monitoring and future comparisons. Therefore, it is important to maintain monitoring of mercury levels in fish species in different marine regions, especially those species most consumed by humans.

Bacterial diversity changes in agricultural soils influenced by poultry litter fertilization.

Poultry litter is widely applied as agricultural fertilizer and can affect the soil microbiome through nutrient overload and antibiotic contamination. In this study, we assessed changes in soil bacterial diversity using high-throughput sequencing approaches. Four samples in triplicate were studied: soils with short- and long-term fertilization by poultry litter (S1 = 10 months and S2 = 30 years, respectively), a soil inside a poultry shed (S3), and a forest soil used as control (S0). Samples S0, S1, and S2 revealed a relatively high richness, with confirmed operational taxonomic units (OTUs) in the three replicates of each sample ranging from 1243 to 1279, while richness in S3 was about three times lower (466). The most abundant phyla were Proteobacteria, Bacteroidetes, and Actinobacteria. Acidobacteria, Planctomycetes, and Verrucomicrobia were also abundant but highly diminished in S3, while Firmicutes was less abundant in S0. Changes in bacterial communities were very evident at the genera level. The genera Gaiella, Rhodoplanes, Solirubacter, and Sphingomonas were predominant in S0 but strongly decreased in the other soils. Pedobacter and Devosia were the most abundant in S1 and were diminished in S2, while Herbiconiux, Brevundimonas, Proteiniphilum, and Petrimonas were abundant in S2. The most abundant genera in S3 were Deinococcus, Truepera, Rhodanobacter, and Castellaniella. A predictive analysis of the metabolic functions with Tax4Fun2 software suggested the potential presence of enzymes associated with antibiotic resistance as well as with denitrification pathways, indicating that the S3 soil is a potential source of nitrous oxide, a powerful greenhouse gas.

Persistence, bioaccumulation and vertical transfer of pollutants in long-finned pilot whales stranded in Chilean Patagonia.

Long-finned pilot whales (LFPW) are cetaceans with strong social groups often involved in mass strandings worldwide. However, these beachings occur for reasons that are not fully understood. In 2016, 124 LFPW were stranded on the Chilean Patagonian islands, offering a unique opportunity to obtain crucial information on the ecology, biology, and genetics of this population. In addition, we examined whether persistent organic pollutants (POPs) and trace elements (TEs) were responsible for this mass mortality. Stable isotopes (δ13C & δ15N) and genetic analyses were used to reconstruct the trophic ecology, social structure, and kinship of LFPW and compared to POPs and TEs levels found in LFPW. Mitochondrial DNA analyses on 71 individuals identified four maternal lineages within the stranded LFPW. Of these animals, 32 individuals were analyzed for a suite of POPs, TEs, and lipid content in blubber. The highest levels were found for ΣDDXs (6 isomers) (542.46 ± 433.46 ng/g, lw) and for total Hg (2.79 ± 1.91 mg/kg, dw). However, concentrations found in these LFPW were lower than toxicity thresholds and those reported for LFPW stranded in other regions. Evidence was found of ΣDDX, Σ7PCBs, and Cd bioaccumulation and maternal transfer of POPs in mother/offspring groups. Nevertheless, no clear relationship between contaminant concentrations and LFPW mortality was established. Further research is still needed to assess LFPW populations including conservations status and exposure to chemicals in remote areas such as Patagonia.

Biomonitoring mercury contamination using fur from roadkilled giant anteaters.

The giant anteater (Myrmecophaga tridactyla) can occur in both preserved and anthropogenic environments in Brazilian biomes. The Cerrado biome, where the samples were collected, is considered one of the world's biodiversity hotspots; however, a large part of it has been converted to pasture and agricultural land. In this environment, animals may be exposed to contaminants, such as mercury (Hg). Despite its immense biodiversity, there are few studies investigating Hg contamination in terrestrial mammals in Brazil. This study aimed to create a baseline for Hg levels in giant anteater fur and establish which biotic and abiotic variables can influence its concentration. Total Hg (THg) concentrations were determined by cold vapor atomic absorption spectrometry in 141 individuals sampled on highways between January and October 2017, in the state of Mato Grosso do Sul, Brazil. THg concentrations in fur ranged from 0.27 to 4.77 µg g -1. The decomposition stage of the carcasses and vehicular traffic volume on highways did not influence the THg concentrations. Juveniles presented lower THg concentrations than adults. A spatial pattern of higher concentrations in the eastern-southeastern region of the state was found, which could be related to anthropogenic impacts; however, no clear links have been established. The THg concentrations in giant anteaters' fur seems higher than expected considering the trophic position of the species. Despite this, the range was in accordance with studies of other Brazilian terrestrial mammals and within safe threshold levels. This study indicates the potential of utilizing roadkilled fauna to monitor large-scale contamination in wildlife.

First year after the Brumadinho tailings' dam collapse: Spatial and seasonal variation of trace elements in sediments, fishes and macrophytes from the Paraopeba River, Brazil.

On January 2019, the B1 iron ore tailings' dam collapsed in Brumadinho, Brazil, being one of the worst mining-related disasters, with 270 human deaths (11 of them still missing) and 12.106 m3 of tailings released to the environment. The tailings devastated the Córrego do Feijão brook and reached the adjacent Paraopeba River, the region's main watercourse and a major tributary of the São Francisco basin. Although physicochemical parameters of the river were strongly impacted, and acute toxicological effects have been reported from exposure experiments, contamination of aquatic biota had not yet been assessed. Therefore, the aim of this study was to evaluate contamination by trace elements (As, Al, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb and Zn) in sediment, fish and macrophytes along the Paraopeba River, upstream and downstream from the dam failure site, during the dry and wet season. With the exception of Cd and Hg, all elements in sediment samples had lower median concentrations downstream. An inverse pattern was observed for the aquatic biota, with significant higher concentrations of Fe, Mn, Ni and Zn in fishes, and increased concentrations of most elements in macrophytes, indicating an increase in element bioavailability. A significant seasonal variation was observed with increased concentrations of As (dry season) and Pb (wet season) in fish samples, with the same trend occurring in macrophytes. Concentrations of potentially toxic elements in fish samples in wet weight (Cr: 1.80 ± 1.31 mg kg-1, Hg: 0.21 ± 0.11 mg kg-1 and Pb: 0.79 ± 0.80 mg kg-1) were lower than those reported before the disaster. Furthermore, As and Pb concentrations exceeded the safety threshold for fish consumption in 3% and 41% of samples, respectively, representing a matter of concern for public health.

An upwelling area as a hot spot for mercury biomonitoring in a climate change scenario: A case study with large demersal fishes from Southeast Atlantic (SE-Brazil).

Data concerning the monomethylmercury (MeHg) bioaccumulation in marine biota from Southeast Atlantic Ocean are scarce. This study purchased large specimens of demersal fishes from an upwelling region: Warsaw grouper (Epinephelus nigritus), Dusky grouper (Epinephelus marginatus) and Namorado sandperch (Pseudopercis numida). The authors addressed the bioaccumulation and toxicokinetic of mercury in fish organs, and the toxicological risk for human consumption of this metal in the muscle tissues accessed. Additionally, the present study discussed the possible implications of shifts in key variables of the environment related to a climate-changing predicted scenario, to the mercury biomagnification in a tropical upwelling system. The muscle was the main stock of MeHg, although the highest THg concentrations have been found in liver tissue. Regarding the acceptable maximum level (ML = 1 mg kg-1), E. nigritus and E. marginatus showed 22% of the samples above this limit. Concerning P. numida, 77% were above 0.5 mg kg-1, but below the ML. The %MeHg in liver and muscle showed no significative correlations, which suggest independent biochemical pathways to the toxicokinetic of MeHg, and constrains the indirect assessment of the mercury contamination in the edible tissue by the liver analyses. The present study highlights the food web features of a tropical upwelling ecosystem that promote mercury biomagnification. Additionally, recent studies endorse the enhancement of upwelling phenomenon due to the climate global changes which boost the pumping of mercury enriched water to the oceanic upper layer. Therefore, the upwelling areas might be hot spots for MeHg monitoring in marine biota.

Fluoroquinolone-contaminated poultry litter strongly affects earthworms as verified through lethal and sub-lethal evaluations.

Poultry litter is one of the main sources of fluoroquinolones (FQs) in agricultural soils. In this study, our main goal was to investigate FQ-contaminated poultry litter effects on Eisenia andrei earthworms. To achieve this, acute and chronic tests covered several endpoints, such as avoidance, biomass, lethality, reproduction and changes to immune cells. FQs (enrofloxacin and ciprofloxacin) were determined in a poultry litter sample through high performance liquid chromatography with a fluorescence detector. The avoidance test indicates that poultry litter strongly repels earthworms, even at the lowest concentration (50 g kg-1). In the acute test, the lethal concentration of poultry litter to 50% of the earthworms (LC50), was estimated at 28.5 g kg-1 and a significant biomass loss (p < 0.05) occurred at 40 g kg-1. In the chronic test, a significant reproduction effect was observed at 20 g kg-1. Cell typing, density and feasibility indicated significant effects ranging from 5 to 20 g kg-1. A high risk quotient was estimated based on recommended poultry litter applications in field studies. Although FQ contamination in poultry litter and soils has been widely reported in previous studies, this is, to the best of our knowledge, the first toxicological assessment concerning earthworms exposed to FQ-contaminated poultry litter.

Dynamics of Hg and MeHg in the Madeira River basin (Western Amazon) before and after impoundment of a run-of-river hydroelectric dam.

The impact of a hydroelectric run-of-river (RoR) dam construction on the dynamics of total mercury (THg) and methylmercury (MeHg) is of interest to the environment and health of human and wild life. We monitored (May 2010 to October 2018) THg and MeHg in the waters and in the suspended particulate matter (SPM) of the Madeira River and its tributaries (before and after dam construction) to evaluate changes upstream and downstream from the Santo Antonio Hydroelectric Dam (SAHD). We collected 2826 samples of water and SPM at sampling stations upstream (UPMD-01, UPMD-02 and UPMD-03) and downstream the Madeira River (DWMD-04, DWMD-05 and DWMD-06), and Tributaries upstream (Branco River, Jaci-Parana River, Jatuarana-I Igarapé, Contra River, Caripunas Igarapé, Ceara Igarapé, and Teotonio Igarapé) and downstream (Jatuarana-II Igarapé and Belmont Igarapé) from the SAHD and monitored water and the total load of SPM. SPM was significantly higher in the Madeira River (median: 178.63 mg.L-1) than in upstream and downstream tributaries (median: 15.30 mg.L-1); however, the THg and MeHg concentrations were significantly higher in the tributaries (median: 161.14 ng g-1 and 9.03 ng g-1, respectively) than in the mainstream Madeira River (median = 57.06 ng g-1 and 1.63 ng g-1, respectively). THg concentration in the water was significantly higher in the mainstream Madeira River (median = 6.51 ng.L-1) than in the tributaries (median = 2.57 ng.L-1). However, the percentage of methylation in the tributaries (median = 4.9%) was 4-times higher than in the Madeira River (median: 1.3%). The significantly higher MeHg percentages in the tributaries may indicate natural (hydro-bio-geochemical factors) still predominant in this changing landscape of the Western Amazon. So far, the data suggest that this RoR has not yet impacted the THg and MeHg concentrations.

Fluoroquinolones and trace elements in poultry litter: estimation of environmental load based on nitrogen requirement for crops.

Poultry litter soil application contributes to sustainability of agricultural systems and is in accordance with the United Nations Sustainable Development Goals (UN-SDG). Poultry litter recommended rates are based on crop nitrogen (N) needs, however, their application can be a potential source of antibiotics and trace elements overload. The aim of the study was to estimate the role of poultry litter application on soil contamination by fluoroquinolones [enrofloxacin (ENR) and ciprofloxacin (CIP)] and trace elements, based on N requirements for crops. Analytical and sampling techniques were used to estimate the loads from poultry litter application. Only CIP was found in poultry litter samples (283 ± 124 µg kg-1) and its load was estimated to be of 9.89 ± 4.33 g ha-1, for the poultry litter application (35 t ha-1). The estimated loads (g ha-1) of trace elements were: Cr 9.19 ± 3.26, Ni 12.3 ± 4.93, Pb 22.0 ± 8.26, Cu 229 ± 85.6, Mn 691 ± 259 and Zn 1,011 ± 378. These estimates were 900% higher than those recommended by the technical guidance, while N exceeded 600% the recommended application. In order to achieve UN-SDGs, local policies to disseminate knowledge and technologies are required for consolidating sustainable agricultural practices.