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1.
Br J Cancer ; 84(12): 1616-23, 2001 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-11401314

RESUMEN

High-risk human papillomaviruses (HR-HPV) are the necessary cause of cervical carcinomas. To determine whether HPR-HPV DNA detection in primary routine screening could represent a sensitive and reliable technique for the detection of high-grade squamous intraepithelial lesions (HGSIL), laboratory analysis using 2 cytologic techniques (conventional and liquid-based), HPV testing with Hybrid Capture II assay (HC-II), followed by colposcopic examination of women with abnormal cervical finding and/or persistent HR-HPV infection, was conducted in 7932 women who had routine cervical examination. The sensitivity of HPV testing for detecting a histologically proven HGSIL was 100%, higher than that of conventional (68.1%) and liquid-based (87.8%) cytology. The low specificities of 85.6% and 87.3% of HPV testing slightly increased to 88.4% and 90.1% if HPV testing was reserved for woman >30 years old. The quantitative approach provided by the HC-II assay for the assessment of the viral load was not reliable for predicting HGSIL in normal smears. HR-HPV testing could be proposed in primary screening in association with cytology. With conventional cytology it significantly improves the detection of HGSIL. With the use of the same cervical scrape for HPV testing and liquid-based cytology, HR-HPV testing would allow to select positive samples treated in a second time for cytology which gives a good specificity.


Asunto(s)
ADN Viral/análisis , Tamizaje Masivo , Papillomaviridae , Infecciones por Papillomavirus/complicaciones , Infecciones Tumorales por Virus/complicaciones , Displasia del Cuello del Útero/virología , Neoplasias del Cuello Uterino/virología , Adolescente , Adulto , Anciano , Femenino , Humanos , Persona de Mediana Edad , Infecciones por Papillomavirus/diagnóstico , Valor Predictivo de las Pruebas , Sensibilidad y Especificidad , Infecciones Tumorales por Virus/diagnóstico , Neoplasias del Cuello Uterino/diagnóstico , Neoplasias del Cuello Uterino/etiología , Frotis Vaginal , Carga Viral , Displasia del Cuello del Útero/diagnóstico , Displasia del Cuello del Útero/etiología
2.
Diagn Mol Pathol ; 9(3): 145-50, 2000 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10976721

RESUMEN

The reliability of the Hybrid Capture II (HC-II; Digene, Silver Spring, MD, U.S.A.) assay was tested in detecting 18 human Papillomavirus (HPV) types for the screening of cervical lesions. Cytology, HPV testing, colposcopy, and biopsy were used to monitor 204 women with normal smears at the first entry. The median follow-up was 15 months (range, 4-27 months). The primary endpoint was clinical progression defined as the presence of a cervical intraepithelial lesion at the biopsy. In the patient population of 204 HPV-infected women, 81 (39.7%) had a persistent HPV infection at two or three examinations with a final histologic diagnosis of 14 high-grade and 13 low-grade squamous intraepithelial lesions (SIL) within 4 to 22 months. Women with regressive HPV infection did not develop any lesion during the same period. The evaluation of the viral load of high-risk HPV by the HC-II did not represent a sensitive approach to predict the persistence or the apparition of high-grade lesions. Thus, persistent high-risk HPV infection detected with HC-II represents a reliable tool to select populations at risk for the development of high-grade cervical lesions.


Asunto(s)
Carcinoma de Células Escamosas/virología , Cuello del Útero/virología , ADN Viral/aislamiento & purificación , Hibridación de Ácido Nucleico , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/virología , Juego de Reactivos para Diagnóstico , Infecciones Tumorales por Virus/virología , Displasia del Cuello del Útero/virología , Neoplasias del Cuello Uterino/virología , Cervicitis Uterina/virología , Frotis Vaginal , Adolescente , Adulto , Carcinoma de Células Escamosas/epidemiología , Carcinoma de Células Escamosas/etiología , Enfermedad Crónica , Colposcopía , Progresión de la Enfermedad , Femenino , Estudios de Seguimiento , Humanos , Tamizaje Masivo , Persona de Mediana Edad , Reproducibilidad de los Resultados , Factores de Riesgo , Sensibilidad y Especificidad , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/etiología , Carga Viral , Displasia del Cuello del Útero/etiología
3.
Cancer ; 88(6): 1347-52, 2000 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-10717615

RESUMEN

BACKGROUND: Some human papillomaviruses (HPVs) are oncogenic in the cervix and are also associated with benign and malignant proliferations in other organs. Currently, the association of HPV with tumors of the lower respiratory tract is not so clearly defined because the studies are difficult to compare; series of cases reported from different geographic regions have used frozen or formalin fixed samples and a variety of techniques of HPV detection. METHODS: The authors studied the prevalence of HPV in a large series of 185 frozen bronchopulmonary tumor samples with a new solution hybridization technique, Hybrid Capture II assay. This test is largely applied in cervical pathology. Its sensitivity is very close to the sensitivity of PCR. It allows the detection of 18 mucosal HPV types, divided into 1 oncogenic and 1 nononcogenic group. RESULTS: Oncogenic HPV DNA was detected by the Hybrid Capture II assay in 5 cases (2.7%) of 185 (3 males and 2 females). In the rare positive cases detected, the authors could not find any consistent morphologic changes classically associated with HPV infection in anogenital lesions, such as koilocytosis. CONCLUSIONS: Oncogenic HPV DNA is detected in a small proportion of cases of bronchopulmonary carcinoma, and thus HPV infection appears to play a limited role in the tumorigenesis of most lung carcinomas.


Asunto(s)
Neoplasias de los Bronquios/virología , Carcinoma/virología , ADN Viral/análisis , Neoplasias Pulmonares/virología , Papillomaviridae/genética , Adenocarcinoma/virología , Adulto , Anciano , Enfermedades del Ano/virología , Tumor Carcinoide/virología , Carcinoma de Células Escamosas/virología , Núcleo Celular/ultraestructura , Núcleo Celular/virología , Femenino , Enfermedades Urogenitales Femeninas/virología , Humanos , Masculino , Enfermedades Urogenitales Masculinas , Persona de Mediana Edad , Membrana Nuclear/ultraestructura , Membrana Nuclear/virología , Hibridación de Ácido Nucleico/métodos , Papillomaviridae/clasificación , Infecciones por Papillomavirus/patología , Prevalencia , Sensibilidad y Especificidad , Infecciones Tumorales por Virus/patología
4.
Br J Cancer ; 80(9): 1306-11, 1999 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-10424730

RESUMEN

Hybrid Capture II (HC-II) is a commercial human papillomavirus (HPV) detection test designed to detect 18 HPV types divided into high-risk and low-risk groups. We have tested 1647 scrapes from 1518 unselected women attending routine cytological screening by this assay for the detection of histologically proven high-grade lesions. The reliability of this test was also evaluated on 117 fresh cone biopsy samples. HPV DNA has been detected in 400 scrapes (24.3%), 296 containing a high-risk HPV (18.0%). All the smears evocative of high-grade lesions were positive for high-risk HPV, and high-risk HPV were detected in all the 34 cases presenting a histologically proven high-grade lesion and in 68 (97.1%) of the 70 cone biopsy samples showing a high-grade lesion or an invasive carcinoma. Thus, the sensitivity was superior to the sensitivity of cytology (85.3%). Nevertheless, the quantitative approach provided by the HC-II assay for the assessment of the viral load could not clearly distinguish among cases with or without high-grade lesions. Thus this assay is recommended for the screening of high-grade lesions on a large scale, in association with classic cytology.


Asunto(s)
Cuello del Útero/virología , Papillomaviridae/aislamiento & purificación , Adolescente , Adulto , Anciano , ADN Viral/análisis , Femenino , Humanos , Persona de Mediana Edad , Sensibilidad y Especificidad
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