RESUMEN
INTRODUCTION: Human hydatidosis is a prevalent zoonotic disease in the Castilla y León region of Spain. The aim of this study is to investigate the seroprevalence of Echinococcus granulosus infection in this region. METHODS: We studied 4824 serum samples from a random, representative population of healthy individuals from each province of Castilla y León, obtained over one year. An indirect enzyme-immunoassay developed in our laboratory was used to determine the presence of IgG antibodies against Echinococcus granulosus in these samples. RESULTS: IgG antibodies against Echinococcus granulosus were detected in 3.4% (164/4824) of samples studied, with a range of 1.26% to 7.10%, depending on the province. Antibody seroprevalence increased significantly with age, but there was no significant sex-related difference (3.66% men vs. 3.14% women). CONCLUSION: The seroprevalence of Echinococcus granulosus infection in Castilla y León is still high. These data contribute to hydatidosis surveillance within the control program for this disease.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Equinococosis/epidemiología , Echinococcus/inmunología , Inmunoglobulina G/sangre , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Equinococosis/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Seroepidemiológicos , España/epidemiologíaRESUMEN
INTRODUCCIÓN. La hidatidosis es una de las zoonosis más importantes en la Comunidad Autónoma de Castilla y León. Este estudio pretende conocer la seroprevalencia de infección por Echinococcus granulosus en dicha comunidad autónoma. MÉTODOS. Se han estudiado 4.824 muestras de suero pertenecientes a 4.824 personas seleccionadas de forma aleatoria y que constituían una muestra representativa de la población de las provincias de Castilla y León. En cada suero se estudió la presencia de anticuerpos de clase IgG frente a Echinococcus granulosus mediante una prueba de enzimoinmunoanálisis indirecto de fabricación propia. RESULTADOS. Se detectaron anticuerpos de clase IgG frente a Echinococcus granulosus en el 3,40 por ciento de los sueros estudiados (164 positivos de 4.824), oscilando entre el 1,26 y el 7,10 por ciento según la provincia de origen. La seroprevalencia de anticuerpos aumentaba significativamente con la edad. No se observaron diferencias estadísticamente significativas entre las seroprevalencias halladas en mujeres y en varones (3,14 por ciento frente a 3,66 por ciento). CONCLUSIÓN. La seroprevalencia de infección por E. granulosus en la Comunidad Autónoma de Castilla y León es todavía alta. Estos datos de seroprevalencia contribuyen a la vigilancia de la hidatidosis dentro de un programa control de esta enfermedad (AU)
Asunto(s)
Persona de Mediana Edad , Animales , Adolescente , Adulto , Anciano de 80 o más Años , Anciano , Masculino , Femenino , Humanos , España , Estudios Seroepidemiológicos , Anticuerpos Antihelmínticos , Equinococosis , Echinococcus , Inmunoglobulina GRESUMEN
The aim of this study was to evaluate the usefulness of a commercial immunoblot (IgG and IgM BAG-Borrelia blot) in the serologic diagnosis of the early stages of Lyme disease. A total of 42 sera from patients with Lyme disease (24 patients with localized early stage (LES) and 18 patients with disseminated early stage (DES)) and 129 sera from patients with non-Lyme diseases (specificity control sera) were studied. IgG anti-p41 from Borrelia burgdorferi s.l. was present in 95.2% of patients followed by anti-p41/I PBi (16.7%), anti-p100 (9.5%) and anti-OspA (9.5%). IgM anti-p41 was present in 66.7% of patients, p41/iPBi (54.8%) and OspC (33.3%). IgM against p100, OspA and OspC were more frequent in DES patients (16.7%, 27.8% and 44.4%) than in LES patients (0.0%, 4.2% and 25.0%). In 4.8% of the cases no IgG bands were present and in 26.2% no IgM bands were present. With the exception of isolated p41 bands (59.5%), no band pattern exceeded 17%. Using manufacturer's instructions, test sensitivity in diagnosis of the early stage of Lyme disease is 61.9%, specificity 98.4% and positive and negative predictive values 92.8% and 88.8% respectively. Applying the EUCALB 5, 6 or 7 rules sensitivity increased to 73.8% although specificity decreased to 89.9%. Of the 129 specific control sera, 41.8% presented IgG anti-p41 and 10.8% IgM anti-p41. Patients with non-Lyme diseases that presented more IgG and IgM bands were those patients with syphilis (88.2%), patients with anti-HIV antibodies (57.8%) and patients with anti-nuclear antibodies (ANA) (52.3%).
