RESUMEN
Corynebacterium pseudotuberculosis es el agente causal de la linfadenitis caseosa en distintas especies animales. No existen reportes en Argentina de casos clínicos en camélidos. Esta comunicación describe el aislamiento y tipificación de Corynebacterium pseudotuberculosis biotipo ovis en una llama (Lama glama) en Córdoba, Argentina. (AU)
Corynebacterium pseudotuberculosis is the causal agent of caseous lymphadenitis in different animal species. There are no reports in Argentina of clinical cases in camelids. This communication describes the isolation and typing of Corynebacterium pseudotuberculosis biotype ovis in llama (Lama glama) in Córdoba, Argentina. (AU)
Asunto(s)
Animales , Camélidos del Nuevo Mundo , Corynebacterium pseudotuberculosis , Linfadenitis/diagnóstico , Infecciones por Corynebacterium/diagnóstico , AbscesoRESUMEN
The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as being of type A, types C and D were also isolated. Furthermore, a high genetic diversity was observed by PFGE among the type A isolates.
Asunto(s)
Infecciones por Clostridium/veterinaria , Clostridium perfringens/genética , Enfermedades Gastrointestinales/veterinaria , Variación Genética , Enfermedades de las Ovejas/microbiología , Animales , Argentina/epidemiología , Infecciones por Clostridium/epidemiología , Infecciones por Clostridium/microbiología , Clostridium perfringens/aislamiento & purificación , Electroforesis en Gel de Campo Pulsado/veterinaria , Femenino , Enfermedades Gastrointestinales/epidemiología , Enfermedades Gastrointestinales/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Ovinos , Enfermedades de las Ovejas/epidemiologíaRESUMEN
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase ß-subunit gene (rpoB). All available CorynebacteriumrpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies.