Shear bond strength of orthodontic brackets bonded with a new self-adhering flowable resin composite.

OBJECTIVES: This study aims to assess the shear bond strength (SBS) to enamel and the distribution of failure modes of brackets bonded using a new self-adhering flowable resin composite (Vertise Flow, VF), with or without preliminary phosphoric acid etching (PAE). MATERIALS AND METHODS: Eighty extracted premolars were randomly divided into four groups (n = 20): (1) etch-and-rinse adhesive (E&R), PAE/Transbond XT Primer/Transbond XT Paste (3M Unitek); (2) self-etch adhesive (SE), Transbond Plus Self-Etching Primer (3M Unitek)/Transbond XT Paste; (3) VF; (4) PAE/VF. In each group, 10 bracketed teeth were debonded within 30 min, while the remaining teeth were subjected to thermocycling before testing. SBS and adhesive remnant index were recorded. RESULTS: SE measured significantly lower early SBS than PAE/VF. Early SBSs recorded by VF were slightly higher yet statistically similar to those of E&R. Such levels of adhesion were achieved by VF regardless of preliminary PAE. After thermocycling, VF measured the lowest SBS. When debonded early, VF and SE tended to leave less residues on enamel surface than E&R. After thermocycling, the failure pattern changed significantly for VF and PAE/VF specimens that all exhibited adhesive failures at the tooth-bracket interface. CONCLUSIONS: VF achieved early bracket SBSs similar to E&R. Following thermocycling, VF and PAE/VF manifested a significant decrease in SBS. CLINICAL RELEVANCE: Although the simplified handling and the satisfactory early SBS of VF may prompt its use for bracket bonding, the decrease in retention noted after thermocycling warns that the issue of bond durability should be thoroughly addressed prior to endorsing this clinical application of VF.

Bonding and sealing ability of a new self-adhering flowable composite resin in class I restorations.

OBJECTIVES: The aim of this study is to assess by means of shear bond strength tests (SBS), microleakage analysis (µLKG), and scanning electron microscopy (SEM) the bonding potential and sealing ability of a new self-adhering composite resin. MATERIALS AND METHODS: SBS and µLKG of Vertise Flow (VF, Kerr) were measured and compared to the all-in-one adhesive systems G-Bond (GB, GC), AdheSE One (AO, Ivoclar Vivadent), Adper Easy Bond (EB, 3M ESPE), Xeno V (XV, Dentsply), and iBOND (iB, Heraeus Kulzer). For each system, 20 molars were tested for SBS on dentin (n = 10) and enamel (n = 10). For µLKG assessment, 12 premolars per group were selected and small, box-shaped cavities were made. After restoration, the teeth were immersed in 50 wt% silver nitrate solution for 24 h. For each group, 10 randomly selected specimens were processed for leakage calculations, while two of the specimens were examined under SEM. Between-group differences in SBS to dentin and µLKG were assessed using Kruskal-Wallis analysis of variance followed by the Dunn's Multiple Range test. Enamel SBS data were analyzed with one-way ANOVA, followed by the Tukey test. RESULTS: On dentin and enamel, VF recorded the lowest SBS values that were statistically comparable to those measured by GB, iB, and AO. µLKG analysis showed the lowest percentage of stained interface for VF. Significantly greater extent of infiltration was seen for iB and EB. CONCLUSIONS: Although VF resulted in lower bond strengths values on either dental substrate, better marginal sealing ability was visualized in comparison with all-in-one adhesive systems. CLINICAL RELEVANCE: The results of the present study demonstrated satisfactory in vitro outcome of the self-adhering flowable composite resin VF when used to restore class I cavities.

Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.

Human induced pluripotent stem (hiPS) cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES) cells remain unclear. We derived fibroblast-like cells from two hiPS cell lines and show that their phenotypic properties and patterns of DNA methylation were similar to that of mature fibroblasts and to fibroblasts derived from hES cells. iPS-derived fibroblasts (iPDK) and their hES-derived counterparts (EDK) showed similar cell morphology throughout differentiation, and patterns of gene expression and cell surface markers were characteristic of mature fibroblasts. Array-based methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles. DNA methylation analysis of promoter regions associated with extracellular matrix (ECM)-production (COL1A1) by iPS- and hESC-derived fibroblasts and fibroblast lineage commitment (PDGFRß), revealed promoter demethylation linked to their expression, and patterns of transcription and methylation of genes related to the functional properties of mature stromal cells were seen in both hiPS- and hES-derived fibroblasts. iPDK cells also showed functional properties analogous to those of hES-derived and mature fibroblasts, as seen by their capacity to direct the morphogenesis of engineered human skin equivalents. Characterization of the functional behavior of ES- and iPS-derived fibroblasts in engineered 3D tissues demonstrates the utility of this tissue platform to predict the capacity of iPS-derived cells before their therapeutic application.

Influence of resin composite mechanical properties on adhesive microtensile bond strength to dentin.

PURPOSE: To determine the influence of mechanical properties of resin-based composites on the microtensile bond strength to dentin of all-in-one adhesives. MATERIALS AND METHODS: Microtensile bond strengths were measured with the non-trimming technique for the experimental groups: 1) Bond Force/Estelite Σ (Tokuyama); 2) G-Bond Plus (GC)/Estelite Σ; 3) Bond Force/Gradia Direct Anterior (GC);4) G-Bond Plus/Gradia Direct Anterior; 5) Bond Force/Gradia Direct LoFlo (GC); 6) G-Bond Plus/Gradia Direct LoFlo. The following mechanical properties of the resin-based composites were assessed: tensile strength, flexural strength, tensile elastic modulus, shear elastic modulus, Poisson's ratio, Vicker's hardness, contraction stress. Three-dimensional models of microtensile beams were created for finite element analysis of the first principal stress values and distribution in the adhesive layer during microtensile testing. Statistical tests were applied to microtensile bond strength values (two-way ANOVA) and to data from mechanical tests (one-way ANOVA). In all the analyses, the level of significance was set at p < 0.05. RESULTS: While the adhesive did not significantly influence microtensile bond strength, the composite was a significant factor. Regardless of the adhesive, Estelite Σ yielded significantly higher bond strengths than Gradia Direct Anterior and Gradia Direct LoFlo, that were comparable. Adhesive-composite interactions were not statistically significant. Estelite Σ had the highest Vicker's hardness, the highest tensile and shear elastic moduli, the lowest and closest to dentin Poisson's ratio. A more favorable stress distribution in the adhesive layer occurred in the Estelite Σ model. CONCLUSIONS: When comparing the bonding potential of several adhesives with the microtensile technique, the same resin composite should be used in all the experimental groups for building up the coronal portion.

Microtensile vs microshear bond strength of all-in-one adhesives to unground enamel.

PURPOSE: to determine the bond strength to unground enamel of all-in-one adhesives in comparison with an etch-andrinse system and to compare the reliability of microtensile and microshear methods in providing such measurements. MATERIALS AND METHODS: the bonding procedure was performed on enamel of 64 extracted molars. The tested all-inone adhesives were: Bond Force (Tokuyama), AdheSE One (Ivoclar-Vivadent), and Xeno V (Dentsply). Prime&Bond NT (Dentsply) served as control. Microtensile specimens were obtained from 4 teeth per group. Twelve teeth per group were used for microshear testing. Microtensile specimens that failed prior to testing were included in statistical calculations; they were assigned the lowest value measured in the respective group. Failure modes were observed under light microscope and classified (cohesive within substrates, adhesive, mixed). Statistically significant differences in bond strength were assessed among the adhesives within each testing method and between microshear and microtensile data for each adhesive. Failure mode distributions were compared using the chi-square test. RESULTS: all-in-one adhesives had similar microshear and microtensile bond strengths. In both testing methods, the etch-and-rinse system achieved the strongest bond. For all adhesives, significantly higher bond strengths were measured with the microshear test. In microtensile testing, specimens bonded with the etch-and-rinse adhesive exhibited a significantly different distribution of failure modes. The coefficients of variation were extremely high for microtensile bond strength data, particularly of all-in-one adhesives. CONCLUSION: the adhesive potential to intact enamel of recently introduced all-in-one adhesives was inferior to that of an etch-and-rinse system. When testing bond strength to enamel of all-in-one adhesives, microshear testing may be a more accurate method than microtensile.

In vitro evaluation of bonding effectiveness to dentin of all-in-one adhesives.

OBJECTIVES: The study was aimed at assessing the bonding potential of all-in-one adhesives to dentin. METHODS: The microtensile bond strength of the all-in-one adhesives Bond Force (Tokuyama), AdheSE One (Ivoclar-Vivadent), and Xeno V (Dentsply) was measured in comparison with the etch-and-rinse system XP Bond (Dentsply). The ultrastructural characteristics of dentin-adhesive interface were observed under scanning electron microscope (SEM). Twenty human extracted third molars had their mid-coronal dentin exposed and ground with wet 600-grit SiC paper in order to create a standardized smear layer. Bonding procedures were performed according to the manufacturers' instructions and microtensile beams were obtained with the "non-trimming" technique. The bond strengths in MPa were statistically analyzed including pre-test failures as "zero" values (Kolmogorov-Smirnov test, Levene's test, One-Way ANOVA, Tukey's test p<0.05). RESULTS: The following bond strengths were recorded in MPa (mean+/-standard deviation): AdheSE One 31.7+/-21.3; Xeno V 42.8+/-26.4; Bond Force 43.3+/-22.1; XP Bond 51.9+/-18.6. The statistical analysis demonstrated that the bond strengths achieved by Bond Force and Xeno V were similar to that of the etch-and-rinse adhesive, whereas the bond strength of AdheSE One was significantly lower. A distinct hybrid layer with resin tags was seen only in XP Bond specimens. All-in-one adhesives demonstrated a rather superficial interaction with the dentin substrate. CONCLUSIONS: Although the strongest bond to dentin was established by the etch-and-rinse system, however the all-in-one adhesives containing organic solvents reached bond strength levels that were comparable from a statistical point of view.