RESUMEN
INTRODUCTION: Elucidating the molecules involved in the inflammatory process of chronic Chagas disease may allow identification of treatment targets. METHODS: The ex vivo phenotypic expression of chemokine receptors CCR1, CCR3, CCR4, CCR5, CXCR2, CXCR3, CXCR4, and CXCR5 on the CD4+ and CD8+ T-cells of patients with chronic Chagas cardiomyopathy of varying severity was evaluated using flow cytometry. RESULTS: Differential expression of CD4+CCR3+ and CD8+CCR4+ T-cells was observed in patients with mild cardiac involvement compared, respectively, with patients with severe cardiac and asymptomatic forms of Chagas disease. CONCLUSIONS: These receptors are possibly involved in the pathogenesis of chronic Chagas cardiomyopathy.
Asunto(s)
Linfocitos T CD4-Positivos/química , Linfocitos T CD8-positivos/química , Cardiomiopatía Chagásica/sangre , Cardiomiopatía Chagásica/patología , Receptores CCR/sangre , Anciano , Femenino , Citometría de Flujo , Expresión Génica , Humanos , Masculino , Persona de Mediana Edad , Fenotipo , Valores de Referencia , Índice de Severidad de la Enfermedad , Estadísticas no ParamétricasRESUMEN
Abstract INTRODUCTION: Elucidating the molecules involved in the inflammatory process of chronic Chagas disease may allow identification of treatment targets. METHODS: The ex vivo phenotypic expression of chemokine receptors CCR1, CCR3, CCR4, CCR5, CXCR2, CXCR3, CXCR4, and CXCR5 on the CD4+ and CD8+ T-cells of patients with chronic Chagas cardiomyopathy of varying severity was evaluated using flow cytometry. RESULTS: Differential expression of CD4+CCR3+ and CD8+CCR4+ T-cells was observed in patients with mild cardiac involvement compared, respectively, with patients with severe cardiac and asymptomatic forms of Chagas disease. CONCLUSIONS: These receptors are possibly involved in the pathogenesis of chronic Chagas cardiomyopathy.
Asunto(s)
Humanos , Masculino , Femenino , Anciano , Linfocitos T CD4-Positivos/química , Cardiomiopatía Chagásica/patología , Cardiomiopatía Chagásica/sangre , Linfocitos T CD8-positivos/química , Receptores CCR/sangre , Fenotipo , Valores de Referencia , Índice de Severidad de la Enfermedad , Expresión Génica , Estadísticas no Paramétricas , Citometría de Flujo , Persona de Mediana EdadRESUMEN
INTRODUCTION: The relationships between monocytes and lymphocytes through MHC class II molecules and costimulatory, are of utmost importance for the production of an efficient immune response. In this work, we assessed the expression of surface molecules CD80 and CD86 on CD14+HLA-DR+ monocytes from patients with Chagas disease. METHODS:: The study population consisted of 31 patients with chronic clinical forms of Chagas disease. Patient blood samples were cultured in the presence of recombinant cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA). RESULTS:: We found considerable differences in the expression profile of surface molecules involved in antigen presentation. CONCLUSIONS:: CRA and FRA may contribute to host immune response evasion by Trypanozoma cruzi.
