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Parasit Vectors ; 12(1): 119, 2019 Mar 26.
Artículo en Inglés | MEDLINE | ID: mdl-30909975

RESUMEN

BACKGROUND: Canine leishmaniosis (CanL) due to Leishmania infantum is characterized by the development of both cellular and humoral immune responses. The dysfunction of T cell-mediated immunity leads to a lack of proliferation of T cells in response to Leishmania antigens with the consequence of parasite dissemination that seems to be related to a T cell exhaustion mediated by regulatory B cells expressing immunoglobulin D (IgD). The aim of this study was to determine and compare the total serum IgD in dogs with clinical leishmaniosis and in clinically healthy dogs. RESULTS: A total of 147 dog sera were studied. All dogs were tested for L. infantum-specific antibodies by quantitative ELISA. Interferon-gamma (IFN-γ) production was also determined by sandwich ELISA after blood stimulation with L. infantum soluble antigen (LSA) or concanavalin A (ConA). The quantification of total IgD was performed using a human IgD sandwich ELISA quantification set. Dogs were classified in three different groups. Group 1 included 40 clinically healthy non-infected dogs, all serologically negative to L. infantum-specific antibodies and non-producers of IFN-γ upon LSA stimulation. Group 2 included 63 clinically healthy infected dogs that were LSA IFN-γ producers (n = 61) and/or IFN-γ non-producers (n = 2) as well as negative to medium seropositive to L. infantum antigen. Finally, Group 3 included 44 dogs with clinical leishmaniosis (IFN-γ producers, n = 23; and IFN-γ non-producers, n = 21) that were negative to highly positive to L. infantum-specific antibodies. No significant differences were observed when the total IgD concentration was compared within groups. Additionally, total IgD of sick IFN-γ producers and IFN-γ non-producers was not significantly different. Finally, total IgD concentration was not statistically related to demographic parameters such as age, sex and breed. CONCLUSIONS: The results of this study demonstrated that there were no differences between groups in total serum IgD. Total serum IgD does not appear to be a marker of disease in CanL.


Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Enfermedades de los Perros/parasitología , Inmunoglobulina D/inmunología , Leishmania infantum/inmunología , Leishmaniasis Visceral/veterinaria , Animales , Anticuerpos Antiprotozoarios/inmunología , Enfermedades de los Perros/sangre , Enfermedades de los Perros/inmunología , Perros , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Inmunoglobulina D/sangre , Leishmaniasis Visceral/inmunología , Leishmaniasis Visceral/parasitología , Masculino , Estudios Retrospectivos
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