[The antiviral activity of the adamantane derivatives against the influenza virus A (H1N1) pdm2009 model in vivo].

For the first time in vivo, the model of the viral pneumonia in mice was used to study the antiviral activity against influenza A virus (H1N1) pdm09 synthetic derivatives of adamantane series including the amino acid residues and lipoid acid. It was found that the adamantane derivatives with histidine, serine, and lipoid acid could inhibit the rimantadine-resistant strain of the influenza A (H1N1) pdm09. As a result, the lifespan of the mice infected with the virus has increased by 1.6 times with respect to viral control. Thus, the possibility of restoration of antiviral properties of rimantadine both in vitro and in vivo by introducing into its molecular structure new functionally active groups was tested.

[Ophthalmological symptoms in patients with giant pituitary adenomas].

In patients with giant pituitary adenomas, visual dysfunction is a common complaint and the chiasmatic syndrome is the major clinical sign of the disease. Reduced vision should be also considered to be a major factor that influences the quality of life. Early and timely diagnosis of a tumor in the absence of its extracellular growth makes it possible to improve visual function in the postoperative period and the quality of life.

[Invasive giant pituitary adenomas].

7 cases of pituitary adenomas with the pronounced signs of invasive growth, confirmed by MRI, clinical and intraoperative observations. Invasive growth of adenomas was related to neither the structure of the tumor, the degree of anaplasia, or the nature of a generated hormone.

[Ultrasonic examinations of thyroid newgrowths]. / Ul'trazvukovye metodiki issledovaniia ob''emnykh obrazovanii shchitovidnoi zhelezy.

The results of complex ultrasonic, histological and cytological examination of 148 patients with newgrowths thyroid were analyzed. Grey-scale mode, colored and energy Doppler scanning, three-dimensional reconstruction of vessels were used. A new classification of nodular vascular pattern types based on 3-D reconstruction is proposed. The majority of malignant formations were characterized by perinodular hypervascularization and internodular hypovascularization (82.4%), adenomas--by peri- and intranodular hypervascularization (88.9%), colloid nodes--by perinodular hypervascularization (85.5%), the blood flow was absent in simple cysts (85.3%). It was established that complex of these methods increases informative value of ultrasonic examination in diagnosis of thyroid nodular diseases: in malignant neoplasms the sensitivity is 92.9%, specificity--86.1%, diagnostic accuracy--86.9%, in adenomas--93.4%, 79.2% and 82%, in colloid nodes--75.7%, 84.1% and 79.8%, respectively. In cystic masses diagnostic value of Doppler methods didn't change: sensitivity was 90.4%, specificity--63%, diagnostic accuracy--80.5%. The combined ultrasonic examination provides a differential approach to treatment of patients with thyroid nodular masses and decreases the number of unjustified surgical interventions.

[Expression in Escherichia coli of hepatitis B virus polymerase and its functional domains]. / Ekspressiia v Escherichia coli polimerazy virusa gepatita B i ee funktsional'nykh domenov.

The plasmids have been constructed permitting expression of hepatitis B viral polymerase and its functional domains, the catalytic and end ones, as hybrid proteins containing 12 alien amino acids in the N-terminal. Immunoblotting with the rabbit antisera to N- and C-terminals of hepatitis B polymerase amino acid sequence has demonstrated that constructed plasmids determine the synthesis of the corresponding proteins in bacterial cells. HBV polymerase is processed in Escherichia coli cells. The successful expression of HBV polymerase and its functional domains makes possible the detailed study of this protein structure and function.